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1. 发明申请

WO2009018537A2 NOVEL FUNGAL ENZYMES 审中-公开
标题翻译：新的真菌酶
公开(公告)号：WO2009018537A2
公开(公告)日：2009-02-05
申请号：PCT/US2008/071982
申请日：2008-08-01
申请人： DYADIC INTERNATIONAL, INC. , NEDWIN, Glenn , GUSAKOV, Alexander, Vasilievich , PUNT, Peter, J. , VERDOES, Jan, Cornelis , SINITSYN, Arkady, Panteleimonovich , VLASENKO, Elena , HINZ, Sandra Wihelmina, Agnes , GOSINK, Mark , JIANG, Zhijie
发明人： NEDWIN, Glenn , GUSAKOV, Alexander, Vasilievich , PUNT, Peter, J. , VERDOES, Jan, Cornelis , SINITSYN, Arkady, Panteleimonovich , VLASENKO, Elena , HINZ, Sandra Wihelmina, Agnes , GOSINK, Mark , JIANG, Zhijie
IPC分类号： C12N9/88
CPC分类号： C12Y302/01021 , C11D3/38645 , C12N9/2437 , C12N9/2442 , C12N9/2445 , C12N9/2477 , C12N9/248 , C12P7/10 , C12P19/14 , C12Y302/01003 , C12Y302/01004 , C12Y302/01014 , C12Y302/01032 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, deinking and biobleaching of paper and pulp, and treatment of waste streams.
摘要翻译：本发明涉及新型酶及其制备方法。更具体地说，本发明涉及各种真菌酶。描述了编码这些酶的核酸分子，组合物，重组和基因修饰的宿主细胞以及使用方法。本发明还涉及将木质纤维素生物质转化为可降解木质纤维素材料的酶和可发酵糖的新方法，包括提供植物生物质协同释放糖的那些酶的新组合。本发明还涉及使用这些酶的新型酶和组合物以各种其他方法，包括衣物洗涤，洗涤剂方法，纸和纸浆的脱墨和生物漂白以及废物流的处理。

2. 发明申请

WO0179507A3 EXPRESSION-REGULATING SEQUENCES AND EXPRESSION PRODUCTS IN THE FIELD OF FILAMENTOUS FUNGI CHRYSOSPORIUM 审中-公开
标题翻译：真菌真菌菌株表达调控序列及表达产物
公开(公告)号：WO0179507A3
公开(公告)日：2002-02-07
申请号：PCT/NL0100301
申请日：2001-04-17
申请人： EMALFARB MARK AARON , PUNT PETER JAN , ZEIJL CORNELIA MARIA JOHANNA V
发明人： EMALFARB MARK AARON , PUNT PETER JAN , VAN ZEIJL CORNELIA MARIA JOHAN
IPC分类号： C12N15/09 , C12N1/15 , C12N1/21 , C12N9/02 , C12N9/24 , C12N9/42 , C12N15/53 , C12N15/56 , C12N15/80 , C12P19/14 , C12Q1/68
CPC分类号： C12N9/248 , C12N9/0008 , C12N9/24 , C12N9/2437 , C12Y302/01004 , C12Y302/01008 , C12Y302/01091
摘要： The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75 %, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86 % amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.
摘要翻译：本发明涉及对应于家族7和10的Chrysosporium糖基水解酶的新型蛋白质，其分别与SEQ ID No.2和4的氨基酸序列具有70和75％的最小氨基酸同一性，并且与对应于丙三酸甘油醛磷酸脱氢酶与SEQ ID No.6的部分氨基酸序列呈现至少86％的氨基酸同一性。本发明还涉及编码这些蛋白质的核酸序列，特别是涉及调控相应基因表达的启动子序列。用于表达这些基因的优选宿主是真菌，特别是Chrysosporium菌株。

3. 发明专利

CA2155451A1 DNA SEQUENCES ENCODING NOVEL BIOSYNTHETIC INSULIN PRECURSORS AND PROCESS FOR PREPARATION OF INSULIN 未知
公开(公告)号：CA2155451A1
公开(公告)日：1996-02-06
申请号：CA2155451
申请日：1995-08-04
申请人： MESTRIC SILVIJA , PUNT PETER J , VALINGER RADOVAN , DEL HONDEL CEES A M J J VAN
发明人： MESTRIC SILVIJA , PUNT PETER J , VALINGER RADOVAN , VAN DEL HONDEL CEES A M J J
IPC分类号： A61K38/00 , C07H21/04 , C07K2/00 , C07K14/62 , C07K14/65 , C12N15/11 , C12N15/17 , C12N15/80 , C12P21/04 , C12N1/15
摘要： The present invention relates to DNA sequences encoding insulin precursors of formula B-Pg-A, wherein B and A represent B- and A-chains, respectively, and Pg represents modified C-peptide or any number of amino acids comprising at least one glycosylation consensus site. The invention also relates to a process for the preparation of insulin by culturing suitable cells, preferably fungal cells, transformed with a vector comprising DNA sequence encoding insulin precursor of given formula. The invention further relates to DNA sequences encoding pharmaceutical peptides and proteins in which N-glycosylation consensus site is introduced in a non-essential site in any spacer region for higher expression and secretion of proteins.

4. 发明申请

WO2010107303A3 CHRYSOSPORIUM LUCKNOWENSE PROTEIN PRODUCTION SYSTEM 审中-公开
标题翻译：木质素蛋白质蛋白质生产系统
公开(公告)号：WO2010107303A3
公开(公告)日：2010-12-16
申请号：PCT/NL2010000045
申请日：2010-03-16
申请人： DYADIC NEDERLAND B V , PUNT PETER J , BURLINGAME RICHARD PAUL , PYNNONEN CHRISTINE M , VERDOES JACOBA , OLSON PHILLIP T , WERY JAN , VISSER JOHANNES HEINRICH , EMALFARB MARK A , VISSER JACOB
发明人： PUNT PETER J , BURLINGAME RICHARD PAUL , PYNNONEN CHRISTINE M , OLSON PHILLIP T , WERY JAN , VISSER JOHANNES HEINRICH , EMALFARB MARK A , VISSER JACOB , VERDOES JAN CORMELIS
IPC分类号： C12N15/00 , C12N15/09 , C12P21/02 , C12R1/645
CPC分类号： C12N15/80 , C12N1/14 , C12N9/1205 , C12N9/2402 , C12N9/2428 , C12N9/2437 , C12N9/2442 , C12N9/248 , C12N15/01 , C12P21/02 , C12Q1/40 , C12R1/645 , C12Y302/01003 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01014 , C12Y302/01015 , C12Y302/01091 , C12Y304/21062 , C12Y304/23025 , G01N2333/93
摘要： The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20 % of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ß- glucanase and endogenous cellobiohydrolase is less than 20 % of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75 %, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.
摘要翻译：本发明提供了一种新的真菌生产系统，其包含真菌宿主菌株Chrysosporium lucknowense，其中内源性纤维素酶分泌少于Chrysosporium lucknowense菌株UV18-25的内源性纤维素酶分泌的20％。优选地，内源性蛋白酶，内源性β-葡聚糖酶和内源性纤维二糖水解酶的分泌也小于Chrysosporium lucknowense菌株UV 18-25的分泌的20％。此外，提供真菌宿主菌株，其中几个基因已被破坏。根据本发明的另一方面，已经描述了用于纯化高于75％的纯蛋白质的同源和/或异源产生方法，其包括在本发明的菌株中表达编码所述蛋白质的基因。此外，已经公开了生产人造蛋白质混合物的方法，其包括在根据本发明的菌株中表达编码每种所述蛋白质的基因。已经提供了通过应用所述菌株来功能性表达所需酶的菌株的简化筛选的方法。

5. 发明申请

WO2009136791A3 NOVEL PHYTASES 审中-公开
标题翻译：新的植物
公开(公告)号：WO2009136791A3
公开(公告)日：2009-12-30
申请号：PCT/NL2009050246
申请日：2009-05-08
申请人： TNO , PUNT PETER JAN , VAN ZEIJL CORNELIA MARIA JOHAN
发明人： PUNT PETER JAN , VAN ZEIJL CORNELIA MARIA JOHANNA
IPC分类号： A23K1/165 , C12N1/15 , C12N9/16 , C12N15/55
CPC分类号： C12N9/16 , A23K20/189 , A23L29/06
摘要： The present invention provides novel polypeptides having phytase activity, recombinant nuclei acid molecules and vectors encoding said novel phytases, and to host cells transformed with said recombinant nuclei acid molecules and vectors. Also provided is a method of transforming a suitable host cell with polynucleotides encoding the phytases, expression of the transformed polynucleotides and the use of the expressed phytase in feed as a supplement.
摘要翻译：本发明提供了具有肌醇六磷酸酶活性的新型多肽，重组核酸分子和编码所述新型肌醇六磷酸酶的载体，以及用所述重组核酸分子和载体转化的宿主细胞。还提供了用编码肌醇六磷酸酶的多核苷酸转化合适的宿主细胞的方法，转化的多核苷酸的表达以及作为补充物在饲料中使用表达的肌醇六磷酸酶。

6. 发明申请

WO2009136791A2 NOVEL PHYTASES 审中-公开
标题翻译：新的植物
公开(公告)号：WO2009136791A2
公开(公告)日：2009-11-12
申请号：PCT/NL2009/050246
申请日：2009-05-08
申请人： NEDERLANDSE ORGANISATIE VOOR TOEGEPAST- NATUURWETENSCHAPPELIJK ONDERZOEK TNO , PUNT, Peter Jan , VAN ZEIJL, Cornelia Maria Johanna
发明人： PUNT, Peter Jan , VAN ZEIJL, Cornelia Maria Johanna
IPC分类号： A23K1/165 , C12N9/16 , C12N15/55 , C12N1/15
CPC分类号： C12N9/16 , A23K20/189 , A23L29/06
摘要： The present invention provides novel polypeptides having phytase activity, recombinant nuclei acid molecules and vectors encoding said novel phytases, and to host cells transformed with said recombinant nuclei acid molecules and vectors. Also provided is a method of transforming a suitable host cell with polynucleotides encoding the phytases, expression of the transformed polynucleotides and the use of the expressed phytase in feed as a supplement.
摘要翻译：本发明提供具有植酸酶活性的新型多肽，重组核酸分子和编码所述新型植酸酶的载体，以及用所述重组核酸分子和载体转化的宿主细胞。还提供了用编码植酸酶的多核苷酸，转化的多核苷酸的表达和在饲料中使用表达的植酸酶作为补充来转化合适的宿主细胞的方法。

7. 发明申请

WO2009104958A1 PRODUCTION OF ITACONIC ACID 审中-公开
标题翻译：生产酸性酸
公开(公告)号：WO2009104958A1
公开(公告)日：2009-08-27
申请号：PCT/NL2009/050069
申请日：2009-02-16
申请人： NEDERLANDSE ORGANISATIE VOOR TOEGEPAST-NATUURWETENSCHAPPELIJK ONDERZOEK TNO , JORE, Johannes Petrus Maria , PUNT, Peter Jan , VAN DER WERF, Maria Johanna
发明人： JORE, Johannes Petrus Maria , PUNT, Peter Jan , VAN DER WERF, Maria Johanna
IPC分类号： C07K14/38 , C12N15/31 , C12P7/44
CPC分类号： C12N15/80 , C07K14/38 , C12P7/44
摘要： The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporterthat can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbours other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.
摘要翻译：本发明涉及编码线粒体三羧酸转运蛋白的核酸序列，其可用于微生物中衣康酸的生产。所述转运蛋白优选是由位于A.rereus的染色体片段上的核酸编码的蛋白质，其还含有参与衣康酸生物合成和洛伐他汀生物合成的其它基因。而且，载体，宿主和转化的微生物是本发明的一部分。

8. 发明申请

WO0109352A3 USE OF ASPERGILLUS SOJAE AS HOST FOR RECOMBINANT PROTEIN PRODUCTION 审中-公开
标题翻译：用作重组蛋白生产的“ASPERGILLUS”SOJAE作为主机的使用
公开(公告)号：WO0109352A3
公开(公告)日：2001-11-08
申请号：PCT/NL0000544
申请日：2000-07-28
申请人： TNO , HOFFMANN LA ROCHE , HEERIKHUISEN MARGREET , DEN HONDEL CORNELIS VAN , PUNT PETER , BIEZEN NICK VAN , ALBERS ALWIN , VOGEL KURT
发明人： HEERIKHUISEN MARGREET , VAN DEN HONDEL CORNELIS , PUNT PETER , VAN BIEZEN NICK , ALBERS ALWIN , VOGEL KURT
IPC分类号： C12N15/09 , C12N1/14 , C12N1/15 , C12N9/16 , C12N9/58 , C12N15/10 , C12N15/67 , C12N15/80 , C12R1/66
CPC分类号： C12N15/80 , C12N9/58
摘要： A recombinant Aspergillus sojae comprising an introduced acetamidase S ( amdS ) gene as selectable marker is disclosed. An Aspergillus sojae exhibiting growth with medium comprising uracil and fluoro-orotic acid, said Aspergillus sojae further not exhibiting growth on medium comprising uridine and fluoro-orotic acid i.e. said Aspergillus sojae exhibiting uracil auxotrophy, said Aspergillus sojae being unable to utilize uridine, said Aspergillus sojae being pyrG negative, said Aspergillus sojae exhibiting resistance to fluoro-orotic acid, said uracil auxotrophy and said fluoro-orotic acid resistance being relievable upon complementation with an active introduced pyrG gene, is described. The Aspergillus sojae further comprises a nucleic acid sequence encoding a phytase or a protein having phytase activity or any other heterologous protein or polypeptide and can be used for the biotechnological production of said phytase or said other heterologous proteins or polypeptides. Additional mutants exhibiting amended morphology are also disclosed. Methods of producing such expression hosts are described.
摘要翻译：公开了包含引入的乙酰胺酶S（amdS）基因作为选择标记的重组曲霉大肠杆菌。表达与包含尿嘧啶和氟代乳清酸的培养基一起生长的曲霉菌，所述的曲霉大肠杆菌在包含尿苷和氟 - 乳清酸的培养基上进一步不显示生长，即所述的< 表现出尿嘧啶营养缺陷型的曲霉大肠杆菌（Aspergillus sojae），所说的曲霉菌（Aspergillus sojae）不能利用尿苷，所说的曲霉菌（Aspergillus sojae）是pyrG阴性的，所说的Aspergillus sojae 表现出对氟乳清酸的抗性，所述尿嘧啶营养缺陷型和所述氟 - 乳清酸抗性在与活性引入的pyrG基因互补时可缓解。曲霉菌还包含编码植酸酶或具有植酸酶活性的蛋白质或任何其它异源蛋白质或多肽的核酸序列，并可用于生物技术生产所述植酸酶或所述其它异源蛋白质或多肽。还公开了表现出修饰形态的另外的突变体。描述了产生这种表达宿主的方法。

9. 发明申请

WO2010107303A2 NEW FUNGAL PRODUCTION SYSTEM 审中-公开
标题翻译：新的真菌生产系统
公开(公告)号：WO2010107303A2
公开(公告)日：2010-09-23
申请号：PCT/NL2010/000045
申请日：2010-03-16
申请人： DYADIC NEDERLAND B.V. , PUNT, Peter, J. , BURLINGAME, Richard, Paul , PYNNONEN, Christine, M. , VERDOES, Jacoba , OLSON, Phillip,T. , WERY, Jan , VISSER, Johannes, Heinrich , EMALFARB, Mark, A. , VISSER, Jacob
发明人： PUNT, Peter, J. , BURLINGAME, Richard, Paul , PYNNONEN, Christine, M. , OLSON, Phillip,T. , WERY, Jan , VISSER, Johannes, Heinrich , EMALFARB, Mark, A. , VISSER, Jacob
IPC分类号： C12N15/00
CPC分类号： C12N15/80 , C12N1/14 , C12N9/1205 , C12N9/2402 , C12N9/2428 , C12N9/2437 , C12N9/2442 , C12N9/248 , C12N15/01 , C12P21/02 , C12Q1/40 , C12R1/645 , C12Y302/01003 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01014 , C12Y302/01015 , C12Y302/01091 , C12Y304/21062 , C12Y304/23025 , G01N2333/93
摘要： The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20 % of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous β- glucanase and endogenous cellobiohydrolase is less than 20 % of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75 %, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.
摘要翻译：本发明提供了一种新的真菌生产系统，其包含真菌宿主菌株Chrysosporium lucknowense，其中内源性纤维素酶分泌少于Chrysosporium lucknowense菌株UV18-25的内源性纤维素酶分泌的20％。优选地，内源性蛋白酶，内源性β-葡聚糖酶和内源纤维二糖水解酶的分泌也小于Chrysosporium lucknowense菌株UV 18-25的分泌的20％。此外，提供真菌宿主菌株，其中几个基因已被破坏。根据本发明的另一方面，已经描述了用于纯化高于75％的纯蛋白质的同源和/或异源产生方法，其包括在本发明的菌株中表达编码所述蛋白质的基因。此外，已经公开了生产人造蛋白质混合物的方法，其包括在根据本发明的菌株中表达编码每种所述蛋白质的基因。已经提供了通过应用所述菌株来功能性表达所需酶的菌株的简化筛选的方法。

10. 发明申请

WO2009033071A3 NOVEL FUNGAL ENZYMES 审中-公开
标题翻译：新的真菌酶
公开(公告)号：WO2009033071A3
公开(公告)日：2009-04-30
申请号：PCT/US2008075464
申请日：2008-09-05
申请人： DYADIC INTERNATIONAL INC , MEIJ JACOBA V D , EMALFARB MARK , GUSAKOV ALEXANDER VASILIEVICH , PUNT PETER J , SINITSYN ARKADY PANTELEIMONOVI , VLASENKO ELENA , HINZ SANDRA WIHELMINA AGNES , GOSINK MARK , JIANG ZHIJIE
发明人： EMALFARB MARK , GUSAKOV ALEXANDER VASILIEVICH , PUNT PETER J , SINITSYN ARKADY PANTELEIMONOVICH , VLASENKO ELENA , HINZ SANDRA WIHELMINA AGNES , GOSINK MARK , JIANG ZHIJIE , VERDOES JAN CORNELIS
IPC分类号： C07H21/00 , D06L4/40
CPC分类号： C12N9/248 , C07H21/00 , C12N9/2402 , C12N9/2408 , C12N9/2434 , C12N9/2471 , C12Y301/01072 , C12Y302/01023 , C12Y302/01037 , C12Y302/01055 , C12Y302/01139 , C12Y302/01151 , C12Y302/01155 , Y02E50/16 , Y02E50/17
摘要： This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefming, deinking and biobleaching of paper and pulp, and treatment of waste streams.
摘要翻译：本发明涉及新型酶及其制备方法。更具体地说，本发明涉及各种真菌酶。描述了编码这些酶的核酸分子，组合物，重组和基因修饰的宿主细胞以及使用方法。本发明还涉及将木质纤维素生物质转化为可降解木质纤维素材料的酶和可发酵糖的新方法，包括提供植物生物质协同释放糖的那些酶的新组合。本发明还涉及通过细胞壁降解释放细胞内容物的方法。本发明还涉及使用这些酶的新型酶和组合物以各种其他方法，包括衣物洗涤，洗涤剂方法，纸和纸浆的生物制备，脱墨和生物漂白以及废物流的处理。

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