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1. 发明授权

US08067192B2 Methods for detection of botulinum neurotoxin 有权
标题翻译：肉毒杆菌神经毒素检测方法
公开(公告)号：US08067192B2
公开(公告)日：2011-11-29
申请号：US12134092
申请日：2008-06-05
申请人： Markus Kalkum , Karine Bagramyan
发明人： Markus Kalkum , Karine Bagramyan
IPC分类号： G01N33/573
CPC分类号： G01N33/573 , C07K7/08 , C07K14/705 , G01N33/5308 , G01N33/542 , G01N33/569 , G01N2333/32 , G01N2333/33 , G01N2333/38
摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.
摘要翻译：本文提供了用于快速和灵敏检测毒素或酶活性的大型免疫吸附剂表面积测定（ALISSA）。该测定被设计为捕获低数量的毒素或酶分子，并通过荧光或发光底物的转化来测量它们的内在蛋白酶活性。 ALISSA比本领域常规使用的方法显着更快和更灵敏。该测定适用于检测各种具有蛋白水解活性的毒素或酶，如肉毒杆菌神经毒素，炭疽杆菌致死因子，人几丁质酶和烟曲霉蛋白酶。还提供了用于构建和鉴定适用于ALISSA方法的新型发光或荧光底物的方法。

2. 发明授权

US06467328B1 Process and device for detecting microparticle movement 失效
标题翻译：用于检测微粒运动的方法和装置
公开(公告)号：US06467328B1
公开(公告)日：2002-10-22
申请号：US09555068
申请日：2000-07-24
申请人： Markus Kalkum , Markus Kietzmann , Martin Müller
发明人： Markus Kalkum , Markus Kietzmann , Martin Müller
IPC分类号： G01N100
CPC分类号： B01L3/0241 , G01N2035/00237 , G01N2035/1034
摘要： For the detection of the position and/or velocity of microparticles which move from dispensers of a dispensing head (30) to a target, a detector target with two linear bound, electro-acoustic or electro-optic interactive areas which fix a target plane is used. When microparticles impact on the interactive areas, detector signals are determined from which along with the dispensing head position motion parameters of the microparticles are obtained.
摘要翻译：为了检测从分配头（30）的分配器移动到目标的微粒的位置和/或速度，具有固定目标平面的两个线性束缚，电声或电光交互区域的检测器目标是用过的。当微粒冲击交互区域时，确定检测器信号与其一起获得微粒的分配头位置运动参数。

3. 发明申请

US20080085286A1 ASPERGILLUS VACCINE PREPARATION AND METHODS OF MAKING AND USING THEREOF 有权
标题翻译： ASPERGILLUS VACCINE制备及其制备和使用方法
公开(公告)号：US20080085286A1
公开(公告)日：2008-04-10
申请号：US11754252
申请日：2007-05-25
申请人： Markus Kalkum , Diana Diaz-Arevalo , James Ito , Joseph Lyons , Teresa Hong
发明人： Markus Kalkum , Diana Diaz-Arevalo , James Ito , Joseph Lyons , Teresa Hong
IPC分类号： A61K39/00 , A61P43/00
CPC分类号： A61K39/0002
摘要： The present invention relates to compositions and methods in preventing and/or treating diseases caused by Aspergillus. In particular, the present invention is directed to Aspergillus vaccine preparations and methods of making and using thereof.
摘要翻译：本发明涉及预防和/或治疗由曲霉菌引起的疾病的组合物和方法。特别地，本发明涉及曲霉疫苗制剂及其制备和使用方法。

4. 发明授权

US07109479B2 Method and system for mass spectroscopy 有权
公开(公告)号：US07109479B2
公开(公告)日：2006-09-19
申请号：US10952529
申请日：2004-09-28
申请人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
发明人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
IPC分类号： B01D59/44 , H01J49/00
CPC分类号： H01J49/063 , H01J49/0481 , H01J49/161 , H01J49/164
摘要： A system for determining the ratio of mass to charge of an ion including a pulsed ionizer, a high pressure co-linear ion guide/accelerator, and a mass analyzer. The pulsed ionizer generates intact analyte ions from a sample of matter to be analyzed. The high pressure co-linear ion guide/accelerator is interfaced with the ion source for receipt of the intact ions of the sample. The ion guide/accelerator simultaneously dampens and linearly accelerates the intact ions in the substantial absence of fragmentation of the ions to provide a substantially continuous beam of the intact ions for mass analysis. The mass analyzer is connected to the ion guide/accelerator for receipt of the beam of ions and determines the mass to charge ratio of the intact ions.

5. 发明授权

US06599479B1 Device and procedure for the electrically triggered microdrop release with a dispensing head 有权
标题翻译：带有分配头的电触发微滴释放的装置和程序
公开(公告)号：US06599479B1
公开(公告)日：2003-07-29
申请号：US09586199
申请日：2000-06-02
申请人： Markus Kietzmann , Markus Kalkum , Thomas Przewieslik , Holger Eickhoff , Carsten Arold
发明人： Markus Kietzmann , Markus Kalkum , Thomas Przewieslik , Holger Eickhoff , Carsten Arold
IPC分类号： B01L302
CPC分类号： G01N35/1074 , B01J2219/00605 , B01J2219/00659 , B01L3/0268 , B01L2200/02 , B01L2200/025 , B01L2200/14 , B01L2400/0439 , B01L2400/0487 , B01L2400/0666 , G01N35/1065 , G01N35/1072 , G01N2015/0088 , G01N2035/1039 , Y10S73/04 , Y10T436/2575
摘要： A multi-channel dispensing head including a plurality of micropipettes, each micropipette having an electrically actuatable trigger device with a ground and signal terminal; and a shared carrier having a plurality of receptacles located in a one- or two-dimensional arrangement and sized and shaped to receive said micropipettes, each receptacle having a ground and signal contact, wherein the ground and signal contacts on the carrier are spaced apart in the direction of a longitudinal axis extending through the respective micropipettes and each of the ground and signal contacts on the carrier contacting the ground and signal terminals of the trigger devices, respectively.
摘要翻译：一种多通道分配头，其包括多个微量移液管，每个微量移液管具有带有接地和信号端子的电致动触发装置; 以及共享载体，其具有位于一维或二维排列中的多个容器，其尺寸和形状适于容纳所述微量移液器，每个插座具有接地和信号接触，其中载体上的接地和信号接触间隔开延伸穿过相应的微量移液管的纵向轴线的方向和载体上的接地和信号触点中的每一个分别接触触发装置的接地端子和信号端子。

6. 发明授权

US08753831B2 Methods for detection of botulinum neurotoxin 有权
标题翻译：肉毒杆菌神经毒素检测方法
公开(公告)号：US08753831B2
公开(公告)日：2014-06-17
申请号：US13600186
申请日：2012-08-30
申请人： Markus Kalkum , Karine Bagramyan
发明人： Markus Kalkum , Karine Bagramyan
IPC分类号： G01N21/76 , G01N21/64 , G01N33/53
CPC分类号： G01N33/5308 , C07K7/08 , C07K14/705 , G01N33/5306 , G01N33/542 , G01N33/569 , G01N33/573 , G01N2333/32 , G01N2333/33 , G01N2333/38 , G01N2333/90241
摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.
摘要翻译：本文提供了用于快速和灵敏检测肉毒杆菌神经毒素（BoNT）和炭疽毒素的大型免疫吸附剂表面积测定（ALISSA）。该测定设计用于捕获少量的毒素分子并通过转化荧光底物或发光底物测量其内在的蛋白酶活性。本文还提供了可以被BoNT特异性切割的新型肽和对BoNT切割具有抗性的新型肽。这些可切割和对照肽的组合可用于实施用于特异性检测BoNT酶活性的示例性ALISSA。此外，如本文所述的ALISSA也可以以基于列的格式使用，以用于高通量系统中用于测试大量样品。

7. 发明授权

US08449894B2 Aspergillus vaccine preparation and methods of making and using thereof 有权
标题翻译：曲霉疫苗制剂及其制备和使用方法
公开(公告)号：US08449894B2
公开(公告)日：2013-05-28
申请号：US11754252
申请日：2007-05-25
申请人： Markus Kalkum , Diana Diaz-Arevalo , James I. Ito , Joseph M. Lyons , Teresa B. Hong
发明人： Markus Kalkum , Diana Diaz-Arevalo , James I. Ito , Joseph M. Lyons , Teresa B. Hong
IPC分类号： A61K39/00 , C07K1/00 , C07K14/00 , C07K17/00
CPC分类号： A61K39/0002
摘要： The present invention relates to compositions and methods in preventing and/or treating diseases caused by Aspergillus. In particular, the present invention is directed to Aspergillus vaccine preparations and methods of making and using thereof.
摘要翻译：本发明涉及预防和/或治疗由曲霉菌引起的疾病的组合物和方法。特别地，本发明涉及曲霉疫苗制剂及其制备和使用方法。

8. 发明授权

US07105357B1 Method and device for processing extremely small substance quantities 有权
标题翻译：用于加工极少量物质的方法和装置
公开(公告)号：US07105357B1
公开(公告)日：2006-09-12
申请号：US09701203
申请日：1999-05-27
申请人： Markus Kalkum , Martin Müller , Eckhardt Nordhoff , Holger Eickhoff , Holger Rauth , Richard Reinhardt
发明人： Markus Kalkum , Martin Müller , Eckhardt Nordhoff , Holger Eickhoff , Holger Rauth , Richard Reinhardt
IPC分类号： G01N1/10 , G01N35/553 , B01L3/02 , B01L11/00
CPC分类号： G01N35/0098 , G01N2035/1039 , G01N2035/1041 , G01N2035/1053 , Y10T436/2575
摘要： For processing of substances in the reservoir (3) of a microdroplet dosing device (1), movement of a solid carrier material with a binding-active surface takes place in the reservoir, and binding of the substance takes place on the surface of the carrier material which comprises magnetic particles (7) or a carrier pad.
摘要翻译：为了处理微滴计量装置（1）的储存器（3）中的物质，在储存器中发生具有结合活性表面的固体载体材料的移动，并且物质的结合发生在载体的表面上材料包括磁性颗粒（7）或载体垫。

9. 发明申请

US20090176259A1 METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN 有权
标题翻译：检测嗜碱性粒细胞毒素的方法
公开(公告)号：US20090176259A1
公开(公告)日：2009-07-09
申请号：US12134092
申请日：2008-06-05
申请人： Markus Kalkum , Karine Bagramyan
发明人： Markus Kalkum , Karine Bagramyan
IPC分类号： G01N33/573 , G01N33/569
CPC分类号： G01N33/573 , C07K7/08 , C07K14/705 , G01N33/5308 , G01N33/542 , G01N33/569 , G01N2333/32 , G01N2333/33 , G01N2333/38
摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorigenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.
摘要翻译：本文提供了用于快速和灵敏检测毒素或酶活性的大型免疫吸附剂表面积测定（ALISSA）。该测定被设计为捕获少量的毒素或酶分子，并通过转化荧光或发光底物来测量它们的内在蛋白酶活性。 ALISSA比本领域常规使用的方法显着更快和更灵敏。该测定适用于检测各种具有蛋白水解活性的毒素或酶，如肉毒杆菌神经毒素，炭疽杆菌致死因子，人几丁质酶和烟曲霉蛋白酶。还提供了用于构建和鉴定适用于ALISSA方法的新型发光或荧光底物的方法。

10. 发明授权

US06822081B2 Process for the isolation and/or purification of a proteinaceous material 有权
标题翻译：用于分离和/或纯化蛋白质材料的方法
公开(公告)号：US06822081B2
公开(公告)日：2004-11-23
申请号：US09760379
申请日：2001-01-16
申请人： Holger Rauth , Richard Reinhardt , Eckhard Nordhoff , Markus Kalkum
发明人： Holger Rauth , Richard Reinhardt , Eckhard Nordhoff , Markus Kalkum
IPC分类号： C12Q100
CPC分类号： C07K1/20 , C07K1/30
摘要： The invention relates to a process for the isolation and/or purification of a proteinaceous material, wherein a solid phase comprising a mixture of hydrophobic and hydrophilic groups is used.
摘要翻译：本发明涉及一种用于分离和/或纯化蛋白质物质的方法，其中使用包含疏水基团和亲水基团的混合物的固相。

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