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1. 发明公开

EP0222869A1 EXPRESSION VECTOR 失效
标题翻译：表达载体。
公开(公告)号：EP0222869A1
公开(公告)日：1987-05-27
申请号：EP86903492.0
申请日：1986-05-30
申请人： Kingsman, Alan John , Kingsman, Susan Mary
发明人： Kingsman, Alan John , Kingsman, Susan Mary
IPC分类号： C07K14 , C12N15
CPC分类号： C12N15/81 , C07K14/56
摘要： Vecteur d'expression de levure comprenant un promoteur et une séquence d'activateur en aval, la séquence d'activateur en aval étant positionnée par rapport au promoteur de manière à en stimuler l'activité, cette même séquence étant positionnée dans le vecteur de sorte que la translation de la séquence d'activateur en aval n'a pas lieu. La séquence d'activateur en aval de levure de phosphoglycérate kinase (PGK) est illustrée en combinaison avec une séquence d'activateur en aval d'oligonucléotide synthétique possédant la séquence de nucléotide ACCAAACGGTGAAAGAAACA. Le promoteur et la séquence d'activateur en aval peuvent être homologues (par exemple promoteur PGK/séquence d'activateur en aval PGK ou hétérologues (par exemple promoteur TRP/séquence d'activateur en aval PGK.

2. 发明公开

EP0073635A3 Expression vectors 失效
标题翻译：表达向量
公开(公告)号：EP0073635A3
公开(公告)日：1984-05-16
申请号：EP82304460
申请日：1982-08-24
申请人： Kingsman, Alan John , Kingsman, Susan Mary
发明人： Kingsman, Alan John
IPC分类号： C12N15/00 , C12P21/00 , C12N01/18 , C12R01/865
CPC分类号： C07K14/56 , C12N15/81 , Y10S930/142
摘要： There are described a number of plasmid vectors suitable for the expression of genetic material, at various levels in yeasts. The plasmids each comprise a yeast selective marker, a yeast replication origin and a yeast promoter positioned relative to a unique restriction site in such a way that expression may be obtained of a polypeptide coding sequence inserted at the restriction site. The promoters used are derived from the 5' region of a gene coding for a yeast glycolytic enzyme e.g. phosphoglycerate kinase (PGK), or from the 5' region of the yeast TRP1 gene. In one Example a plasmid contains a promoter derived from both the 3' and 5' regions of the PGK gene. The replication systems used involve the yeast 2µ replication origin or an autonomous replicating sequence (ARS) stabilised with an ARS stabilising sequence (ASS). The replication systems allow for a choice of high or low copy number per cell. The promoter sequences allow for a choice of high or low expression level. A kit including vectors having a combination of these alternative features is described. Yeast expression vectors including a gene for coding for human interferon-a are described.

3. 发明公开

EP0144408A1 DNA SEQUENCE 失效
标题翻译： DNA序列。
公开(公告)号：EP0144408A1
公开(公告)日：1985-06-19
申请号：EP84902353.0
申请日：1984-05-31
申请人： Kingsman, Alan John , Kingsman, Susan Mary
发明人： Kingsman, Alan John , Kingsman, Susan Mary
IPC分类号： C12N15 , C12N1
CPC分类号： C12N15/81
摘要： Séquence d'activateur en amont dérivée du gène PGK de levure. La séquence d'activateur en amont est contenue dans la région 5' du gène PGK de levure entre les nucléotides -324 et -455. La séquence d'activateur en amont possède des éléments de liaison synthétiques liés à chaque extrémité pour faciliter la liaison d'un vecteur. Des vecteurs comprenant la séquence d'activateur en amont et une séquence de promoteur hétérologue sont décrits. La séquence d'activateur en amont est utilisée dans un procédé permettant d'accroître le niveau d'expression d'un vecteur d'expression de levure.

4. 发明公开

EP0156851A1 YEAST HYBRID VECTORS 审中-实审
标题翻译：酵母双杂交载体。
公开(公告)号：EP0156851A1
公开(公告)日：1985-10-09
申请号：EP84903492.0
申请日：1984-09-19
申请人： Kingsman, Alan John , Kingsman, Susan Mary
发明人： Kingsman, Alan John , Kingsman, Susan Mary
IPC分类号： C12N15 , C12N1 , C12P21 , C12R1
CPC分类号： C12N15/81
摘要： Un vecteur de levure comprend l'un ou l'autre ou les deux gènes REP1, REP2 dérivés du plasmide 2mu de levure, situé par rapport à une séquence de régulation capable d'assurer l'expression de l'un ou des deux gènes REP1, REP2 où la séquence de régulation comprend un activeur qui agit sensiblement indépendamment du nombre de reproductions du vecteur dans un organisme hôte transformé avec le vecteur. La séquence de régulation comprend un activeur contrôlable tel que l'activeur PGK de levure qui permet le contrôle du nombre de reproductions des vecteurs d'expression transformés en commun avec le vecteur de levure. Le vecteur de levure, qui peut être intégré dans le génome chromosomique de levure, est utilisé dans un procédé de production d'un polypeptide dans lequel la production du polypeptide est maintenue à un faible niveau jusqu'à atteindre une densité cellulaire de culture prédéterminée; à ce point on augmente le nombre de reproductions du vecteur d'expression et/ou le niveau d'expression en modifiant les conditions de culture.

5. 发明专利

GB2157295A YEAST HYBRID VECTORS 未知
公开(公告)号：GB2157295A
公开(公告)日：1985-10-23
申请号：GB8511107
申请日：1984-09-19
申请人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
发明人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
IPC分类号： C12N15/00 , C12N1/16 , C12N1/19 , C12N15/09 , C12N15/81 , C12P21/02 , C12R1/865
摘要： A yeast vector including either or both of the REP1 and/or REP2 gene(s) derived from the yeast 2 mu plasmid, located relative to a control sequence capable of directing expression of either or both of the REP1 and/or REP2 gene(s) in which the control sequence includes a promoter which operates substantially independently of vector copy number in a host organism transformed with the vector. The control sequence includes a controllable promoter such as the yeast PGK promoter which allows for copy number control of expression vectors cotransformed with the yeast vector. The yeast vector, which may be integrated into the yeast chromosomal genome, is used in a process for producing a polypeptide in which the production of polypetide is maintained at a low level until a predetermined culture cell density is achieved at which point the expression vector copy number and/or the expression level are caused to increase by altering the culture conditions.

6. 发明专利

GB2150577A DNA SEQUENCE 未知
公开(公告)号：GB2150577A
公开(公告)日：1985-07-03
申请号：GB8502503
申请日：1984-05-31
申请人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
发明人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
IPC分类号： C12N15/00 , C12N1/21 , C12N15/09 , C12N15/81
摘要： PCT No. PCT/GB84/00189 Sec. 371 Date Jan. 30, 1985 Sec. 102(e) Date Jan. 30, 1985 PCT Filed May 31, 1984 PCT Pub. No. WO84/04757 PCT Pub. Date Dec. 6, 1984.An upstream activator sequence derived from the yeast PGK gene. The upstream activator sequence is contained in the 5' region of the yeast PGK gene between nucleotides -324 and -455. The upstream activator sequence has synthetic linkers attached to either end to facilitate attachment to a vector. Vectors including the upstream activator sequence and a heterologous promoter sequence are described. The upstream activator sequence is used in a method for increasing the expression level of a yeast expression vector.

7. 发明专利

GB9703802D0 Anti-hiv peptides and proteins 未知
公开(公告)号：GB9703802D0
公开(公告)日：1997-04-16
申请号：GB9703802
申请日：1997-02-24
申请人： KINGSMAN SUSAN M , KINGSMAN ALAN J
IPC分类号： C12N15/09 , A61K31/711 , A61K39/00 , A61K48/00 , A61P31/18 , C07K14/16 , C12N15/48
摘要： This invention relates to novel peptides and proteins and nucleic acids encoding them, which are useful against HIV infection. The peptides comprise an amino acid sequence of a part of the HIV-1 p17 protein or of the HIV-2 p16 protein, from amino acid residues 31 to 45 or from amino acid residues 41 to 55. The proteins are recombinant p16 and p17 proteins having an alteration in helix A which is defined by amino acid residues 31 to 46, or the A-B loop which is defined by amino acid residues 47 to 52.

8. 发明专利

DK368882A 未知
公开(公告)号：DK368882A
公开(公告)日：1983-02-26
申请号：DK368882
申请日：1982-08-17
申请人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
发明人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
IPC分类号： C12N15/09 , C07H21/04 , C07K14/56 , C12N1/16 , C12N15/00 , C12N15/81 , C12P21/00 , C12R1/865 , C12N
摘要： There are described a number of plasmid vectors suitable for the expression of genetic material, at various levels in yeasts. The plasmids each comprise a yeast selective marker, a yeast replication origin and a yeast promoter positioned relative to a unique restriction site in such a way that expression may be obtained of a polypeptide coding sequence inserted at the restriction site. The promoters used are derived from the 5' region of a gene coding for a yeast glycolytic enzyme e.g. phosphoglycerate kinase (PGK), or from the 5' region of the yeast TRP1 gene. In one Example a plasmid contains a promoter derived from both the 3' and 5' regions of the PGK gene. The replication systems used involve the yeast 2µ replication origin or an autonomous replicating sequence (ARS) stabilised with an ARS stabilising sequence (ASS). The replication systems allow for a choice of high or low copy number per cell. The promoter sequences allow for a choice of high or low expression level. A kit including vectors having a combination of these alternative features is described. Yeast expression vectors including a gene for coding for human interferon-a are described.

9. 发明专利

GB2157295B YEAST HYBRID VECTORS 未知
公开(公告)号：GB2157295B
公开(公告)日：1987-10-07
申请号：GB8511107
申请日：1984-09-19
申请人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
发明人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
IPC分类号： C12N15/00 , C12N1/16 , C12N1/19 , C12N15/09 , C12N15/81 , C12P21/02 , C12R1/865

10. 发明专利

GB2150577B DNA SEQUENCE 未知
公开(公告)号：GB2150577B
公开(公告)日：1987-02-11
申请号：GB8502503
申请日：1984-05-31
申请人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
发明人： KINGSMAN ALAN JOHN , KINGSMAN SUSAN MARY
IPC分类号： C12N15/00 , C12N1/21 , C12N15/09 , C12N15/81
摘要： PCT No. PCT/GB84/00189 Sec. 371 Date Jan. 30, 1985 Sec. 102(e) Date Jan. 30, 1985 PCT Filed May 31, 1984 PCT Pub. No. WO84/04757 PCT Pub. Date Dec. 6, 1984.An upstream activator sequence derived from the yeast PGK gene. The upstream activator sequence is contained in the 5' region of the yeast PGK gene between nucleotides -324 and -455. The upstream activator sequence has synthetic linkers attached to either end to facilitate attachment to a vector. Vectors including the upstream activator sequence and a heterologous promoter sequence are described. The upstream activator sequence is used in a method for increasing the expression level of a yeast expression vector.

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