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1. 发明申请

US20100062091A1 PROCESSED PRODUCT OF FENUGREEK SEEDS AND METHOD FOR PRODUCING THE SAME 有权
标题翻译：植物种子的加工产品及其生产方法
公开(公告)号：US20100062091A1
公开(公告)日：2010-03-11
申请号：US12596406
申请日：2008-04-17
申请人： Yuki Nakano , Shohei Hoshino , Jinji Shono , Nobuaki Tsuge
发明人： Yuki Nakano , Shohei Hoshino , Jinji Shono , Nobuaki Tsuge
IPC分类号： A61K36/48 , A61P3/04 , A61P3/10 , C12N9/99
CPC分类号： C12P1/00 , A23L11/33 , A23L33/105 , A61K36/48
摘要： It is an object of the present invention to enhance the α-amylase inhibitory activity of a fenugreek seed component. According to the present invention, the bitter taste of fenugreek seeds can be collaterally reduced.The present invention relates to a processed product of fenugreek seeds obtained by allowing β-glucosidase to act on a fenugreek seed-derived material and the like, and the method for producing the same.
摘要翻译：本发明的目的是提高葫芦巴种子成分的α-淀粉酶抑制活性。根据本发明，葫芦巴种子的苦味可以相应减少。本发明涉及通过使β-葡糖苷酶作用于葫芦巴种子衍生物质等而得到的葫芦巴种子的加工品及其制造方法。

2. 发明申请

US20050022263A1 DNA and vector for repressing expression of gene of lachrymatory factor-producing enzyme, method for repressing expression of gene of lachrymatory factor-producing enzyme with them and vegetables having repressed expression of gene of lachrymatory factor-producing enzyme 失效
标题翻译：用于抑制产生Lachrymatory因子的酶基因表达的DNA和载体，用它们抑制产生lachrymative因子的酶基因表达的方法和产生产生Lachrymatory因子的酶的基因的表达的蔬菜
公开(公告)号：US20050022263A1
公开(公告)日：2005-01-27
申请号：US10932950
申请日：2004-09-01
申请人： Shinsuke Imai , Nobuaki Tsuge , Yasuhiro Kamata , Noriya Masamura , Jinji Shono , Kentaro Horie
发明人： Shinsuke Imai , Nobuaki Tsuge , Yasuhiro Kamata , Noriya Masamura , Jinji Shono , Kentaro Horie
IPC分类号： C12N9/90 , C12N15/29 , C12N15/82 , A01H1/00 , C07H21/04 , C12N5/04 , C12N9/02
CPC分类号： C12N15/8243 , C12N9/90 , C12N15/8242
摘要： The object of the present invention is to provide DNA and RNA designed on the basis of the sequence of an enzyme gene for repressing the expression of the enzyme gene generating a lachrymatory factor from a precursor of the lachrymatory factor, and also a vector required for introducing DNA for repressing the expression of the gene of the lachrymatory factor-producing enzyme into a vegetable. The present invention relates to DNA comprising at least one sequence selected from the following sequences and a regulatory sequence connected to said sequence so as to make the transcription possible: (a) a DNA sequence encoding a protein or a polypeptide of a lachrymatory factor-producing enzyme or a part of the DNA sequence in a sense orientation, an antisense orientation or both the orientations, wherein the protein or the polypeptide has an effect of converting 1-propenylsulfenic acid into the lachrymatory factor; (b) a regulatory sequence of a DNA in a vegetable genome determined on the basis of the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the regulatory sequence in a sense orientation, an antisense orientation or both the orientations; and (c) a DNA sequence located between the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme and the regulatory sequence of the DNA in the vegetable genome DNA determined on the basis of the DNA encoding the protein or the polypeptide of the lachrymatory factor-producing enzyme or a part of the DNA sequence in a sense orientation, an antisense orientation or both the orientations.
摘要翻译：本发明的目的是提供基于酶基因序列设计的DNA和RNA，其用于抑制来自产生泪液因子的前体的产生泪液因子的酶基因的表达，以及引入所需的载体用于将产生致命菌素的酶的基因的表达抑制成植物的DNA。本发明涉及包含至少一个选自以下序列的序列的DNA，以及与所述序列连接的调控序列，以使转录成为可能：（a）编码产生产生泪液的因子的蛋白质或多肽的DNA序列酶或一部分DNA序列在正义取向，反义方向或取向两者中，其中所述蛋白质或多肽具有将1-丙烯基亚磺酸转化为变应原因子的作用; （b）基于编码产生产生泪液因子的酶的蛋白质或多肽的DNA或有义方向，反义方向或两者的一部分调节序列确定的植物基因组DNA的调节序列方向; 和（c）位于编码产生致命菌素的酶的蛋白质或多肽的DNA与植物基因组DNA中的DNA的调节序列之间的DNA序列，其基于编码所述蛋白质或多肽的DNA 衍生因子产生酶或部分DNA序列在正向，反义方向或取向上。

3. 发明申请

US20100055241A1 FENUGREEK SEED HAVING REDUCED BITTER TASTE AND METHOD FOR PRODUCING THE SAME 审中-公开
标题翻译：具有减少叮咬味道的柚子种子及其生产方法
公开(公告)号：US20100055241A1
公开(公告)日：2010-03-04
申请号：US12450906
申请日：2008-04-18
申请人： Yuki Nakano , Shohei Hoshino , Jinji Shono , Nobuaki Tsuge
发明人： Yuki Nakano , Shohei Hoshino , Jinji Shono , Nobuaki Tsuge
IPC分类号： A23L1/36 , A23L1/211
CPC分类号： A23L25/20 , A23L11/33
摘要： It is an object of the present invention to obtain fenugreek seeds having reduced bitter taste without causing significant changes in non-bitter components contained in fenugreek seeds.The present invention relates to a method for producing fenugreek seeds having reduced bitter taste, comprising allowing β-glucosidase to act on an eluate in which the components of fenugreek seeds have been eluted and then allowing the fenugreek seeds to absorb the eluate and β-glucosidase, and a food containing such fenugreek seeds.
摘要翻译：本发明的目的在于获得葫芦巴种子具有降低的苦味，而不会引起葫芦巴种子中所含的非苦味成分的显着变化。本发明涉及一种生产具有降低苦味的葫芦巴种子的方法，其特征在于包括使葡萄糖苷酶作用于洗脱液中，其中胡芦巴种子的组分已被洗脱，然后使胡芦巴种子吸收洗脱液和葡萄糖苷酶和含有这种葫芦巴种子的食物。

4. 发明授权

US07452988B2 Vector for suppression of lachrymatory factor synthase (LFs) in transgenic onions 失效
标题翻译：用于抑制转基因洋葱中的泪液因子合成酶（LFs）的载体
公开(公告)号：US07452988B2
公开(公告)日：2008-11-18
申请号：US10932950
申请日：2004-09-01
申请人： Shinsuke Imai , Nobuaki Tsuge , Tsunchiro Kamata , Noriya Masamura , Jinji Shono , Kentaro Horie
发明人： Shinsuke Imai , Nobuaki Tsuge , Tsunchiro Kamata , Noriya Masamura , Jinji Shono , Kentaro Horie
IPC分类号： C07H21/04 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8243 , C12N9/90 , C12N15/8242
摘要： The present invention provides DNA and RNA designed on the basis of the sequence of an enzyme gene for repressing the expression of the enzyme gene generating a lachrymatory factor from a precursor of the lachrymatory factor, and also a vector for introducing DNA for repressing the expression of the gene of the lachrymatory factor-producing enzyme into a vegetable.
摘要翻译：本发明提供了基于用于抑制来自产生泪液因子的前体的产生泪液因子的酶基因的表达的酶基因的序列设计的DNA和RNA，以及用于引入DNA以抑制表达的表达的载体将产生Lachrymatory因子的酶的基因转变成植物。

5. 发明授权

US08287927B2 Processed product of fenugreek seeds and method for producing the same 有权
标题翻译：葫芦巴种子的加工产品及其生产方法
公开(公告)号：US08287927B2
公开(公告)日：2012-10-16
申请号：US12596406
申请日：2008-04-17
申请人： Yuki Nakano , Shohei Hoshino , Jinji Shono , Nobuaki Tsuge
发明人： Yuki Nakano , Shohei Hoshino , Jinji Shono , Nobuaki Tsuge
IPC分类号： A61K36/00
CPC分类号： C12P1/00 , A23L11/33 , A23L33/105 , A61K36/48
摘要： It is an object of the present invention to enhance the α-amylase inhibitory activity of a fenugreek seed component. According to the present invention, the bitter taste of fenugreek seeds can be collaterally reduced.The present invention relates to a processed product of fenugreek seeds obtained by allowing β-glucosidase to act on a fenugreek seed-derived material and the like, and the method for producing the same.
摘要翻译：本发明的目的是提高葫芦巴种子成分的α-淀粉酶抑制活性。根据本发明，葫芦巴种子的苦味可以相应减少。本发明涉及通过使β-葡糖苷酶作用于葫芦巴种子衍生物质等而得到的葫芦巴种子的加工品及其制造方法。

6. 发明申请

US20110256283A1 Method for Preventing Decomposition/Deterioration of Lipophilic Component in the Presence of Water 审中-公开
标题翻译：防止亲水性成分在水中分解/变质的方法
公开(公告)号：US20110256283A1
公开(公告)日：2011-10-20
申请号：US13141285
申请日：2009-12-24
申请人： Yasuharu Hashimoto , Jinji Shono , Akiko Kamoi , Nobuaki Tsuge , Masaki Nakamura , Tadashi Hamajima , Morihiro Aoyagi , Nobuhide Nakagawa
发明人： Yasuharu Hashimoto , Jinji Shono , Akiko Kamoi , Nobuaki Tsuge , Masaki Nakamura , Tadashi Hamajima , Morihiro Aoyagi , Nobuhide Nakagawa
IPC分类号： A23L3/3454
CPC分类号： A61K8/738 , A23L27/18 , A23L27/75 , A23V2002/00 , A61K8/361 , A61K8/63 , A61K2800/52 , A61Q19/00 , A23V2250/5112 , A23V2250/2136 , A23V2250/18
摘要： Provided is a method for preventing decomposition/deterioration of a lipophilic component due to interaction with water, or due to interaction with light, enzymes, oxygen, or heat in the presence of water. The method for preventing decomposition/deterioration of the lipophilic component in the presence of water is characterized in that a complex comprising a lipophilic component, a phytosterol ester, and a cyclodextrin is formed, and the aforementioned lipophilic component is preserved in the form of said complex in the presence of water.
摘要翻译：本发明提供一种防止由于与水的相互作用而引起的亲油性成分的分解/变质，或者由于与光，酶，氧或热在水的存在下的相互作用而引起的分解/劣化的方法。在水存在下防止亲油性成分的分解/变质的方法的特征在于，形成包含亲油性成分，植物甾醇酯和环糊精的配合物，上述亲油性成分以所述配合物的形式保存在水的存在下。

7. 发明申请

US20080064028A1 Quantitative Pcr Method of Detecting Specific Plant Genus in Food or Food Ingredient 审中-公开
标题翻译：检测食品或食品成分中特定植物属的定量Pcr方法
公开(公告)号：US20080064028A1
公开(公告)日：2008-03-13
申请号：US10556903
申请日：2004-05-14
申请人： Takashi Hirao , Masayuki Hiramoto , Satoshi Watanabe , Jinji Shono
发明人： Takashi Hirao , Masayuki Hiramoto , Satoshi Watanabe , Jinji Shono
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6895 , C12Q1/686 , C12Q2561/113
摘要： Provided is a method of quantifying a specific plant genus in a food or a food ingredient by a PCR method, comprising: (i) preparing a sample for correction where a sample derived from the specific plant genus to be detected and a standard plant sample are mixed in a predetermined ratio, and extracting genomic DNA from the sample for correction; (ii) preparing a test sample where a known amount of the standard plant sample is added to the food or the food ingredient to be examined, and extracting genomic DNA from the test sample; (iii) practicing a quantitative PCR method using the genomic DNAs and primers; and (iv) conducting correction with a standard value for correction determined for the sample for correction to calculate the amount of the specific plant ingredient contained in the test sample.
摘要翻译：本发明提供了一种通过PCR方法对食品或食品成分中的特定植物属进行定量的方法，其特征在于，包括：（i）制备用于校正的样品，其中来源于要检测的特定植物属的样品和标准植物样品为以预定比例混合，并从样品中提取基因组DNA进行校正; （ii）制备测试样品，其中将已知量的标准植物样品加入到待检查的食品或食品成分中，并从测试样品中提取基因组DNA; （iii）使用基因组DNA和引物进行定量PCR方法; 和（iv）进行用于校正的样品的校正标准值的校正，以计算测试样品中包含的特定植物成分的量。

8. 发明申请

US20050026210A1 Protein or polypeptide having lachrymatory factor-producing enzymatic activity, DNA coding for said protein or polypeptide, method for preparation of the protein or polypeptide having lachrymatory factor-producing enzymatic activity by using said DNA and nucleic acid molecule having a function of repressing translation of mRNA with respect to said protein or polypeptide 审中-公开
标题翻译：具有产生产生lachrymative因子的酶活性的蛋白质或多肽，编码所述蛋白质或多肽的DNA，通过使用所述DNA和具有抑制mRNA翻译功能的核酸分子来制备具有产生lachrymatory因子的酶活性的蛋白质或多肽的方法相对于所述蛋白质或多肽
公开(公告)号：US20050026210A1
公开(公告)日：2005-02-03
申请号：US10932970
申请日：2004-09-01
申请人： Shinsuke Imai , Nobuaki Tsuge , Yasuhiro Kamata , Noriya Masamura , Jinji Shono
发明人： Shinsuke Imai , Nobuaki Tsuge , Yasuhiro Kamata , Noriya Masamura , Jinji Shono
IPC分类号： C12N9/90 , C12N15/29 , C12N15/82 , C12Q1/68 , A01H1/00 , C07H21/04 , C12N5/04 , C12N9/02
CPC分类号： C12N15/8243 , C12N9/90 , C12N15/8242
摘要： The present invention relates to protein or polypeptide capable of converting 1-propenylsulfenic acid concerning the formation of lachrymatory factors generated when plants such as onions are broken into pieces or cut into lachrymatory factors, and also to DNA (lachrymatory factor-producing enzyme genes) encoding the protein or polypeptide; a primer usable for isolating a gene of a lachrymatory factor-producing enzyme of the genus allium and a method for isolating the gene with the primer; a recombinant vector containing the DNA; a transformant containing the recombinant vector containing the DNA; a method for preparing protein or a polypeptide having lachrymatory factor-producing enzymatic activity by culturing host cells transformed with the recombinant vector containing the DNA; protein or a polypeptide having the lachrymatory factor-producing enzymatic activity; and a nucleic acid molecule having a function of repressing the translation of mRNA on protein or polypeptide having the lachrymatory factor-producing enzymatic activity.
摘要翻译：本发明涉及能够转化1-丙烯基亚磺酸的蛋白质或多肽，其涉及当诸如洋葱等植物破碎成碎片或切割成泪液因子时产生的变应性因子的形成，以及编码的DNA（产生lachrymatory因子的酶基因）蛋白质或多肽; 一种可用于分离该属的产生致命因子的酶的基因的引物和一种用该引物分离该基因的方法; 含有DNA的重组载体; 含有含有该DNA的重组载体的转化体; 通过培养用含有该DNA的重组载体转化的宿主细胞来制备蛋白质或具有产生产生lachrymative因子的酶活性的多肽的方法; 蛋白质或具有产生致命因素的酶活性的多肽; 以及具有抑制具有产生产生产生产生产生泪液因子的酶活性的蛋白质或多肽上的mRNA的翻译的功能的核酸分子。

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