专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2007106425A3 BIOMARKERS OF THE DYSPLASTIC STATE OF CELLS 审中-公开
标题翻译：细胞滋养状态的生物标志物
公开(公告)号：WO2007106425A3
公开(公告)日：2008-10-16
申请号：PCT/US2007006176
申请日：2007-03-12
申请人： UNIV NORTHEASTERN , CYTYC CORP , WU SHIAW-LIN , HANCOCK WILLIAM S , KARGER BARRY L , LINDER JAMES , HANLON DAVID W
发明人： WU SHIAW-LIN , HANCOCK WILLIAM S , KARGER BARRY L , LINDER JAMES , HANLON DAVID W
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： G01N33/57411
摘要： The invention relates to methods for detecting and identifying potential biomarkers of high-grade cervical dysplasia in an individual human subject. The invention also relates to newly discovered biomarkers, as set forth in Tables 1-4 herein, which are associated with the dysplastic state of cervical cells. It has been discovered that a differential level of expression of any of these markers or combination of these markers correlates with a dysplastic condition in a human subject, e.g., a patient.
摘要翻译：本发明涉及用于检测和鉴定个体人类受试者高级宫颈发育异常的潜在生物标志物的方法。本发明还涉及新发现的与本发明的表1-4所示的生物标志物，其与子宫颈细胞的发育不良状态相关。已经发现，这些标记物或这些标记物的组合的表达差异水平与人受试者例如患者中的发育异常状况相关。

2. 发明申请

WO2006044666A3 DETECTION OF DISEASE ASSOCIATED PROTEOLYSIS BY DIRECT MASS SPECTROMETRY ANALYSIS OF LOW MOLECULAR WEIGHT PEPTIDES 审中-公开
公开(公告)号：WO2006044666A3
公开(公告)日：2006-04-27
申请号：PCT/US2005/037024
申请日：2005-10-17
申请人： NORTHEASTERN UNIVERSITY , HANCOCK, William, S. , BAKER, Haven , HINCAPIE, Marina , ZHENG, Xiaoyang
发明人： HANCOCK, William, S. , BAKER, Haven , HINCAPIE, Marina , ZHENG, Xiaoyang
IPC分类号： G01N24/00 , G01N30/02
摘要： A method for detecting disease associated proteolysis based on direct mass spectrometric analysis of low molecular weight peptides from biological sample representing the collective degradome, is disclosed. The molecular weight range of the peptides is from approx. 400 to approx. 12,000 DA. Low molecular weight peptides are isolated from the biological samples by reverse phase chromatography, ultrafiltration or bulk electrophoresis. Preferable embodiments comprise using a hybrid ion-trap mass spectrometer coupled to a quadropule, time-of-flight mass spectrometer. The method comprises identifying specific peptides having elevated levels compared to a reference normal, correlating peptides with specific proteins and identifying protein fragmentation cleavage sites and relating these cleavage sites to the activity of a specific endogenous propeolytic enzyme.

3. 发明申请

WO2006044666A2 DETECTION OF DISEASE ASSOCIATED PROTEOLYSIS 审中-公开
标题翻译：疾病相关蛋白的检测
公开(公告)号：WO2006044666A2
公开(公告)日：2006-04-27
申请号：PCT/US2005037024
申请日：2005-10-17
申请人： UNIV NORTHEASTERN , HANCOCK WILLIAM S , BAKER HAVEN , HINCAPIE MARINA , ZHENG XIAOYANG
发明人： HANCOCK WILLIAM S , BAKER HAVEN , HINCAPIE MARINA , ZHENG XIAOYANG
IPC分类号： G01N33/48
CPC分类号： G01N33/6848
摘要： Described herein are methods and techniques to study the "degradome". The degradome of a specific protease is the complete product of the natural substrate repertoire of that enzyme in a cell, tissue or organism. The complete set of proteases that are expressed at a particular moment or circumstance by a cell, tissue or organism produces the collective degradome. Included in the methods described herein are approaches that allow the direct identification and characterization of degradome peptides from approx. 400 to approx. 12,000 Da. The methods of the invention avoid the inherent problems of studying the peptidome by focusing on specific or unique proteolytic cleavages that occur as a result of endogenous protease activity induced by specific diseases. Once characterized, the presence of, or change in level of, specific peptides of the degradome can be used, e.g., to identify specific peptides having elevated levels compared to a reference normal/or to correlate identified peptides with specific proteins and/or to identify protein fragmentation patterns (e.g., peptide ladders) and the specific protease(s) that brought them about and then correlate this information with the presence or absense of a specific disease or condition. Thus, the methods of the invention can be used, for example, to identify new diagnostic markers and/or therapeutic targets, as specific clinical diagnostic methods for individual patients and as methods of monitoring the progress of a therapeutic regimen for the treatment of a patient.
摘要翻译：本文描述了研究“降解物”的方法和技术。特定蛋白酶的降解物是细胞，组织或生物体中该酶的天然底物的全部产物。在细胞，组织或生物体的特定时刻或环境下表达的完整蛋白酶产生集体降解物。包括在本文所描述的方法中的方法是允许直接鉴定和表征降解物肽的大约。 400到约 12,000 Da。本发明的方法通过集中于由特定疾病诱导的内源性蛋白酶活性而产生的特异性或独特的蛋白水解裂解来避免研究肽酶的固有问题。一旦表征，可以使用降解物的特异性肽的存在或水平的变化，例如，鉴定与参考标准相比具有升高的水平的特异性肽，或将鉴定的肽与特异性蛋白质相关联和/或鉴定蛋白质断裂模式（例如，肽梯）和特异性蛋白酶，使其与特定疾病或病症的存在或缺失相关联。因此，本发明的方法可用于例如鉴定新的诊断标记物和/或治疗靶标，作为个体患者的特定临床诊断方法，以及监测用于治疗患者的治疗方案进展的方法。

4. 发明申请

WO2006026569A2 COMPREHENSIVE CHARACTERIZATION OF COMPLEX PROTEINS AT TRACE LEVELS 审中-公开
标题翻译：复杂蛋白质的综合表征
公开(公告)号：WO2006026569A2
公开(公告)日：2006-03-09
申请号：PCT/US2005030713
申请日：2005-08-29
申请人： UNIV NORTHEASTERN , WU SHIAW-LIN , HANCOCK WILLIAM S , KARGER BARRY L
发明人： WU SHIAW-LIN , HANCOCK WILLIAM S , KARGER BARRY L
IPC分类号： G01N33/50
CPC分类号： G01N33/6848 , G01N33/6842
摘要： An LC-MS platform, Extended Range Proteomic Analysis, which is able to achieve very high sequence coverage and comprehensive characterization of posttranslational modifications in complex proteins at the trace level (e.g., low pmole to fmole), is described. The platform according to the invention provides advantages of both the top-down and bottom-up proteomic approaches by combining, In a preferred embodiment of the method, (i) digestion of the protein with an enzyme, such as Lys-C, that cuts less frequently than trypsin, or limited digestion with, e.g., trypsin, leading to, on average, a higher molecular weight peptide size with greater than 90% of the protein's peptide backbone sequence contained in fragments that are between 500 and 25,000 Da; (ii) high-performance LC separation of these resulting fragments; (iii) a new data acquisition strategy using on-line coupling of specific separated fragments to analysis in, e.g., the LTQ-FTMS, a hybrid mass spectrometer that couples a linear ion trap with a Fourier transform ion cyclotron resonance (FTICR) cell, for peptide analysis, preferably of the fragments in the range of 3000 to 10,000 Da; and (iv) new data analysis methods for assigning large peptide structures and determining the site of attachment of posttranslational modifications as well as structural features from the accurate precursor mass together with MS
摘要翻译：描述了能够在痕量水平（例如低pmole至fmole）的复杂蛋白质中实现非常高的序列覆盖率和翻译后修饰的全面表征的LC-MS平台，扩展范围蛋白质组学分析。根据本发明的平台通过组合来提供自顶向下和自下而上的蛋白质组学方法的优点。在该方法的优选实施方案中，（i）将蛋白质与诸如Lys-C的酶消化，平均来说，胰蛋白酶的平均消化速度比胰蛋白酶低，或者限制在500到25,000Da之间的片段中含有大于蛋白质肽主链序列的90％的分子量肽; （ii）这些得到的片段的高效液相色谱分离; （iii）使用特定分离片段的在线耦合来分析例如LTQ-FTMS，将线性离子阱与傅立叶变换离子回旋共振（FTICR）单元耦合的混合质谱仪的新数据采集策略，用于肽分析，优选在3000至10,000Da范围内的片段; 和（iv）用于分配大肽结构并确定翻译后修饰的附着位点的新数据分析方法以及来自准确前体质量的结构特征以及MS

5. 发明申请

WO2005107491A3 MULTI-LECTIN AFFINITY CHROMATOGRAPHY AND USES THEREOF 审中-公开
标题翻译：多重色谱亲和色谱及其用途
公开(公告)号：WO2005107491A3
公开(公告)日：2005-12-22
申请号：PCT/US2005015919
申请日：2005-05-05
申请人： UNIV NORTHEASTERN , HANCOCK WILLIAM S , YANG ZIPING , HINCAPIE MARINA
发明人： HANCOCK WILLIAM S , YANG ZIPING , HINCAPIE MARINA
IPC分类号： A23J1/00 , C07K1/22 , C07K17/08 , C07K17/14 , G01N33/53 , G01N33/68
CPC分类号： G01N33/6842 , C07K1/22 , G01N2333/4724 , G01N2400/00
摘要： Methods, compositions, and kits related to the use of multi-ligand affinity chromatography are described. The methods include those related to identification of glycoprotein panels for analyzing and diagnosisng disease.
摘要翻译：描述了与使用多配体亲和层析相关的方法，组合物和试剂盒。这些方法包括与识别用于分析和诊断疾病的糖蛋白板相关的方法。

6. 发明申请

WO2007106425A2 PROTEOMIC METHODS FOR THE IDENTIFICATION AND USE OF PUTATIVE BIOMARKERS ASSOCIATED WITH THE DYSPLASTIC STATE IN CERVICAL CELLS OR OTHER CELL TYPES 审中-公开
标题翻译：用于鉴定和使用与细胞中的浮游状态或其他细胞类型相关的引诱生物标记物的预防方法
公开(公告)号：WO2007106425A2
公开(公告)日：2007-09-20
申请号：PCT/US2007/006176
申请日：2007-03-12
申请人： NORTHEASTERN UNIVERSITY , CYTYC CORPORATION , WU, Shiaw-Lin , HANCOCK, William, S. , KARGER, Barry, L. , LINDER, James , HANLON, David, W.
发明人： WU, Shiaw-Lin , HANCOCK, William, S. , KARGER, Barry, L. , LINDER, James , HANLON, David, W.
IPC分类号： C12Q1/68
CPC分类号： G01N33/57411
摘要： The invention relates to methods for detecting and identifying potential biomarkers of high-grade cervical dysplasia in an individual human subject. The invention also relates to newly discovered biomarkers, as set forth in Tables 1-4 herein, which are associated with the dysplastic state of cervical cells. It has been discovered that a differential level of expression of any of these markers or combination of these markers correlates with a dysplastic condition in a human subject, e.g. , a patient.
摘要翻译：本发明涉及用于检测和鉴定个体人类受试者高级宫颈发育异常的潜在生物标志物的方法。本发明还涉及新发现的与本发明的表1-4所示的生物标志物，其与子宫颈细胞的发育不良状态相关。已经发现，这些标记物或这些标记物的组合的差异水平的表达与人类受试者的发育不良状况相关，例如，，病人。

7. 发明申请

WO2005077106A3 MONOCLONAL ANTIBODY BASED BIOMARKER DISCOVERY AND DEVELOPMENT PLATFORM 审中-公开
标题翻译：基于单克隆抗体的生物识别发现与发展平台
公开(公告)号：WO2005077106A3
公开(公告)日：2006-02-02
申请号：PCT/US2005004484
申请日：2005-02-09
申请人： UNIV NORTHEASTERN , TAKACS LASZLO , GUTTMAN ANDRAS , HANCOCK WILLIAM S , KARGER BARRY L , DUVAL MANUEL , BERNA PATRICK
发明人： TAKACS LASZLO , GUTTMAN ANDRAS , HANCOCK WILLIAM S , KARGER BARRY L , DUVAL MANUEL , BERNA PATRICK
IPC分类号： B01L3/00 , C07K16/18 , G01N33/68 , G01N33/53 , A61K39/395 , C12M1/34 , C12N5/12
CPC分类号： G01N33/6854 , B01L3/5027 , C07K16/18 , G01N33/54386 , G01N33/577 , G01N33/6845 , G01N2500/00
摘要： A method or platform for monoclonal antibody based biomarker discovery is disclosed. The method according to the invention provides for the integration of analyte collection, hybridoma screening and nanovolume integrated mass spectrometry (NVIMS) to achieve a robust screening system that is capable, for example, of cutting 4-6 years off of the classical biomarker discovery and development process. The invention provides a platform for the rapid, high-throughput production, isolation and characterization of, e.g., disease specific biomarkers together with highly specific monoclonal antibodies. The method of the invention has a variety of applications such as, but not limited to, drug testing, biohazard applications, ecological applications, physilogical applications and/or pathology screening applications. The method of the invention is also capable of being performed or used as or with a high-throughput screening process or system of the invention.
摘要翻译：公开了用于基于单克隆抗体的生物标志物发现的方法或平台。根据本发明的方法提供了分析物收集，杂交瘤筛选和纳瓦级综合质谱（NVIMS）的整合，以实现强大的筛选系统，其能够例如从经典生物标志物发现开始4-6年，开发过程。本发明提供了用于快速，高通量生产，分离和表征例如疾病特异性生物标志物以及高度特异性单克隆抗体的平台。本发明的方法具有多种应用，例如但不限于药物测试，生物危害应用，生态应用，物理应用和/或病理学筛选应用。本发明的方法还能够作为或与本发明的高通量筛选方法或系统一起使用或使用。

8. 发明申请

WO2005107491A2 MULTI-LECTIN AFFINITY CHROMATOGRAPHY AND USES THEREOF 审中-公开
标题翻译：多重凝胶亲和色谱及其用途
公开(公告)号：WO2005107491A2
公开(公告)日：2005-11-17
申请号：PCT/US2005/015919
申请日：2005-05-05
申请人： NORTHEASTERN UNIVERSITY , HANCOCK, William, S. , YANG, Ziping , HINCAPIE, Marina
发明人： HANCOCK, William, S. , YANG, Ziping , HINCAPIE, Marina
IPC分类号： A23J1/00
CPC分类号： G01N33/6842 , C07K1/22 , G01N2333/4724 , G01N2400/00
摘要： Methods, compositions, and kits related to the use of multi-ligand affinity chromatography are described. The methods include those related to identification of glycoprotein panels for analyzing and diagnosisng disease.
摘要翻译：描述了与使用多配体亲和层析有关的方法，组合物和试剂盒。这些方法包括与识别用于分析和诊断疾病的糖蛋白组相关的方法。

9. 发明申请

WO2006026569A3 COMPREHENSIVE CHARACTERIZATION OF COMPLEX PROTEINS AT TRACE LEVELS 审中-公开
公开(公告)号：WO2006026569A3
公开(公告)日：2006-03-09
申请号：PCT/US2005/030713
申请日：2005-08-29
申请人： NORTHEASTERN UNIVERSITY , WU, Shiaw-Lin , HANCOCK, William, S. , KARGER, Barry, L.
发明人： WU, Shiaw-Lin , HANCOCK, William, S. , KARGER, Barry, L.
IPC分类号： G01N24/00 , G01N24/14
摘要： A combination of “bottom up” anf “top down” MS analysis of posstranslational modifications in complex proteins is described. The method comprises digestion of the protein with an enzyme that forms larger peptide fragments than trypsin (> 3000 D), performing HPLC with the fragments and applying a new data acquisition strategy using on-line coupling with e.g. LTQ-FTMS, a hybrid mass spectrometer that couples a linear ion trap with a Fourier transform ion cyclotron resonance (FTICR) cell. The method is applied to analysis of posstranslational modifications of protein isoforms.

10. 发明申请

WO2009006568A1 BIOMARKERS FOR DIABETES, OBESITY, AND/OR HYPERTENSION 审中-公开
标题翻译：糖尿病，肥胖和/或高血压的生物标志物
公开(公告)号：WO2009006568A1
公开(公告)日：2009-01-08
申请号：PCT/US2008/069145
申请日：2008-07-03
申请人： NORTHEASTERN UNIVERSITY , HANCOCK, William, S. , HINCAPIE, Marina , DAYARATHNA, M.K. Disni, R.
发明人： HANCOCK, William, S. , HINCAPIE, Marina , DAYARATHNA, M.K. Disni, R.
IPC分类号： G01N33/53
CPC分类号： G01N33/74 , G01N33/6848 , G01N33/92 , G01N2800/042 , G01N2800/044 , G01N2800/321 , G01N2800/322 , G01N2800/50
摘要： Methods of identifying subjects having, or at risk of developing, diabetes, obesity, and/or hypertension are disclosed, as well as methods of identifying biomarkers for diabetes, obesity, and/or hypertension, and biomarkers identified by such methods.
摘要翻译：公开了识别具有发生糖尿病，肥胖和/或高血压的风险的受试者的方法，以及鉴定糖尿病，肥胖症和/或高血压的生物标志物的方法以及通过这些方法鉴定的生物标志物的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式