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    • 3. 发明公开
    • VERFAHREN ZUR IDENTIFIZIERUNG VON ORGANISMEN DURCH VERGLEICHENDE GENETISCHE ANALYSE SOWIE PRIMER UND HYBRIDISATIONSSONDEN ZUR DURCHFÜHRUNG DES VERFAHRENS
    • 一种用于识别的生物体比较成因分析与引物和杂交探针用于执行该方法
    • EP1169478A2
    • 2002-01-09
    • EP00918802.0
    • 2000-03-16
    • Schackert, Hans KonradHahn, MatthiasGörgens, Heike
    • SCHACKERT, Hans, KonradHAHN, MatthiasKOUFAKI, Olga, NikiGÖRGENS, Heike
    • C12Q1/68
    • C12Q1/6895C12Q1/6876C12Q2600/156
    • The invention comprises methods, primers and hybridisation probes for identifying organisms by means of comparative genetic analysis and is characterised in that coding, non-coding areas and/or functionally significant areas of highly conserved genes, pseudogenes or homologues are amplified using the PCR and then genotyped and analysed. The comparison of coding and non-coding areas of highly conserved genes, pseudogenes or homologues ensures that a single oligonucleotide pair bonds to DNA sequences that are highly conserved between different species, hereby allowing the amplification of a gene segment that is identical for all of the species. The oligonucleotides include one or more gene areas with the greatest possible sequence differences between different species. The determination of the gene sequence of this highly polymorphous area in a subsequent reaction step enables the gene sequence to be allocated to a specific species. In particularly preferred variants of embodiments of the invention, oligonucleotide pairs enabling the amplification of the highly conserved tumour suppresser gene PTEN/MMAC1, its pseudogene and their homologues were found.
    • 用于通过比较基因分析鉴定生物方法包括聚合酶链反应(PCR)扩增和随后的基因分型和编码和/或非编码区的分析,和/或高度保守的基因和/或它们的同源物功能显著区域,和/或 他们的基因拷贝和/或他们的假。 因此独立权利要求中包括了以下内容:(1)寡核苷酸引物(I),用于聚合酶链反应(PCR)和PTEN / MMAC1基因及其同系物的各区域的测序; (2),用于使用至少一对杂交探针的,确定性的采矿不同组合的熔点不同物种的DNA之间进行区分,并收集结果以产生每个种类的面板的方法; (3) '的LightCycler' 杂交探针(II)为外显子5,6,7,8和PTEN / MMAC1基因的9; (4)对特异于猪和人的PTEN假基因杂交探针; (5)的DNA序列(IV)和/或它们的片段,从PTEN / MMAC1基因的同源物和/或它的假没在细胞 - 细胞粘附和细胞周期调控和胚胎发育过程中具有重要的功能涉及编码的蛋白质; (6)MMAC1从PTEN /基因或其假与母(伪)相比其中,基因具有碱基交换,插入和/或缺失,并且是适合于相应种类的识别的同系物的DNA序列; (7)套件的新方法。