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1. 发明授权

US07094879B2 Genetically engineered P30 antigen, improved antigen cocktail, and uses thereof 有权
标题翻译：遗传工程化的P30抗原，改良抗原鸡尾酒及其用途
公开(公告)号：US07094879B2
公开(公告)日：2006-08-22
申请号：US10263153
申请日：2002-10-02
申请人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
发明人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
IPC分类号： C07K1/00 , C07K14/00 , C07K17/00 , G01N33/53
CPC分类号： C07K14/45 , A61K39/00 , G01N33/56905 , G01N2333/45 , G01N2469/20 , Y10S435/975
摘要： The present invention relates to a genetically engineered P30 antigen and a combination or mixture of antigens (e.g., the genetically engineered P30 antigen and P35) that may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using this genetically engineered P30 antigen and combination of antigens, antibodies raised against this genetically engineered P30 antigen and combination of antigens, as well as kits and vaccines containing the genetically engineered P30 antigen and antigens present in the combination.
摘要翻译：本发明涉及可用于检测弓形虫的IgM和/或IgG抗体的遗传工程化的P30抗原和抗原（例如，遗传工程化的P30抗原和P35）的组合或混合物。此外，本发明还涉及使用这种遗传工程化的P30抗原和抗原组合，针对该遗传工程化的P30抗原产生的抗体和抗原组合的方法，以及含有遗传工程化的P30抗原的试剂盒和疫苗以及存在于组合。

2. 发明授权

US5786181A Process for producing high purity 6, 12-dideoxyerythromycin A by fermentation 失效
标题翻译：通过发酵生产高纯度6,12-脱红霉素A的方法
公开(公告)号：US5786181A
公开(公告)日：1998-07-28
申请号：US789979
申请日：1997-01-28
申请人： Diane L. Stassi , Gregory T. Maine , David A. Post , Mark T. Satter
发明人： Diane L. Stassi , Gregory T. Maine , David A. Post , Mark T. Satter
IPC分类号： C12N15/09 , C12N5/10 , C12N9/06 , C12N9/10 , C12N15/76 , C12N15/90 , C12P19/62
CPC分类号： C12N9/0012 , C12N15/76 , C12N15/90 , C12N9/1007 , C12P19/62
摘要： A process for producing high purity 6,12-dideoxyerythromycin A using recombinant DNA technology is disclosed. The erythromycin producing strain, Saccharopolyspora erythraea, lacking the erythromycin C-12 and C-6 hydroxylases produces a mixture of 6,12-dideoxyerythromycin A and the precursor molecule, 6-deoxyerythromycin D. To achieve conversion of the precursor to the final product, a second copy of eryG is inserted into a non-essential region of the Sac. erythraea chromosome resulting in high purity 6,12-dideoxyerythromycin A.
摘要翻译：公开了使用重组DNA技术生产高纯度6,12-二脱氧红霉素A的方法。缺乏红霉素C-12和C-6羟化酶的红霉素产生菌，红酵母，产生6,12-脱羟基红霉素A和前体分子6-脱氧红霉素D的混合物。为了使前体转化成最终产物，将eryG的第二个拷贝插入Sac的非必需区域。导致红细胞染色体高纯度6,12-脱氧红霉素A.

3. 发明授权

US07314924B2 Polynucleotide encoding a genetically engineered P30 antigen 有权
标题翻译：编码遗传工程化的P30抗原的多核苷酸
公开(公告)号：US07314924B2
公开(公告)日：2008-01-01
申请号：US11316532
申请日：2005-12-21
申请人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
发明人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
IPC分类号： C12N15/74 , C07H21/04
CPC分类号： C07K14/45 , A61K39/00 , G01N33/56905 , G01N2333/45 , G01N2469/20 , Y10S435/975
摘要： The present invention relates to a genetically engineered P30 antigen and a combination or mixture of antigens (e.g., the genetically engineered P30 antigen and P35) that may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using this genetically engineered P30 antigen and combination of antigens, antibodies raised against this genetically engineered P30 antigen and combination of antigens, as well as kits and vaccines containing the genetically engineered P30 antigen and antigens present in the combination.
摘要翻译：本发明涉及可用于检测弓形虫的IgM和/或IgG抗体的遗传工程化的P30抗原和抗原（例如，遗传工程化的P30抗原和P35）的组合或混合物。此外，本发明还涉及使用这种遗传工程化的P30抗原和抗原组合，针对该遗传工程化的P30抗原产生的抗体和抗原组合的方法，以及含有遗传工程化的P30抗原的试剂盒和疫苗以及存在于组合。

4. 发明申请

US20050277178A1 EMBEDDED EXPRESSION OF HETEROLOGOUS PROTEINS 失效
标题翻译：杂交蛋白的嵌入表达
公开(公告)号：US20050277178A1
公开(公告)日：2005-12-15
申请号：US08742619
申请日：1996-11-01
申请人： GREGORY T. MAINE , LINDA E. CHOVAN
发明人： GREGORY T. MAINE , LINDA E. CHOVAN
IPC分类号： C12N15/09 , C12N15/62 , C12N15/70
CPC分类号： C12N15/70 , C12N15/62
摘要： A method for expressing a heterologous protein in a prokaryotic host cell, comprising the steps of: (a) providing a DNA vector having: (1) a control region operatively linked to a first genetic element encoding a carrier protein capable of expression in the host cell, and (2) a second genetic element encoding the heterologous protein, the second element being embedded within the first element such that the first and second elements are contiguous and in the same reading frame; (b) transforming the host cell with the DNA vector; and (c) expressing a fusion protein of the heterologous protein and the carrier protein, wherein the heterologous protein is joined at its N-terminus to a first domain of the carrier protein and at its C-terminus to a second domain of the carrier protein, and also a method for confirming intact expression of heterologous proteins, as well as fusion proteins, DNA constructs, plasmid vectors and transformed host cells relating to the above methods.
摘要翻译：一种用于在原核宿主细胞中表达异源蛋白质的方法，包括以下步骤：（a）提供DNA载体，所述DNA载体具有：（1）与编码能够在宿主中表达的载体蛋白的第一遗传元件可操作地连接的对照区细胞，和（2）编码异源蛋白质的第二遗传元件，第二元素嵌入在第一元素内，使得第一和第二元素连续并且位于相同的阅读框中; （b）用DNA载体转化宿主细胞; 和（c）表达异源蛋白质和载体蛋白质的融合蛋白，其中所述异源蛋白质在其N末端连接至载体蛋白质的第一结构域，并且其C末端连接至载体蛋白质的第二结构域，以及用于确认异源蛋白质的完整表达以及与上述方法相关的融合蛋白，DNA构建体，质粒载体和转化的宿主细胞的方法。

5. 发明授权

US5024941A Expression and secretion vector for yeast containing a glucoamylase signal sequence 失效
标题翻译：含有葡糖淀粉酶信号序列的酵母的表达和分泌载体
公开(公告)号：US5024941A
公开(公告)日：1991-06-18
申请号：US810423
申请日：1985-12-18
申请人： Gregory T. Maine , Robert S. Daves , Robert R. Yocum
发明人： Gregory T. Maine , Robert S. Daves , Robert R. Yocum
IPC分类号： C12N15/09 , C12N1/16 , C12N9/26 , C12N9/34 , C12N15/00 , C12P21/00 , C12Q1/04 , C12Q1/14 , C12R1/865
CPC分类号： C12N9/2428 , C12Q1/04 , C12Q1/14 , C07K2319/02
摘要： A vector including a DNA sequence encoding a secretory signal sequence substantially identical to the secretory signal-encoding sequence of a glucoamylase gene from Saccharomyces diastaticus or S. cerevisiae; and upstream from the signal-encoding sequence, a DNA sequence capable of promoting transcription in yeast (e.g., a high yield promoter, such as the promoter of the triose phosphate isomerase gene), transcription of the signal-encoding sequence being under the control of the transcription-promoting sequence, a site for the insertion into the vector of a heterologous DNA sequence, in reading frame with the signal-encoding sequence. The vector is useful as an expression vector in yeast.
摘要翻译：一种载体，其包含编码与来自糖酵母或酿酒酵母的葡糖淀粉酶基因的分泌信号编码序列基本相同的分泌信号序列的DNA序列; 和信号编码序列的上游，能够在酵母中促进转录的DNA序列（例如，高产率启动子，例如磷酸丙糖异构酶基因的启动子），信号编码序列的转录在转录促进序列，用于插入异源DNA序列的载体的位点，在具有信号编码序列的阅读框中。该载体可用作酵母中的表达载体。

6. 发明授权

US07824908B2 Genetically engineered P30 antigen, improved antigen cocktail, and uses thereof 有权
标题翻译：遗传工程化的P30抗原，改良抗原鸡尾酒及其用途
公开(公告)号：US07824908B2
公开(公告)日：2010-11-02
申请号：US11860383
申请日：2007-09-24
申请人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
发明人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
IPC分类号： C12N15/00 , C07H21/04
CPC分类号： C07K14/45 , A61K39/00 , G01N33/56905 , G01N2333/45 , G01N2469/20 , Y10S435/975
摘要： The present invention relates to a genetically engineered P30 antigen and a combination or mixture of antigens (e.g., the genetically engineered P30 antigen and P35) that may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using this genetically engineered P30 antigen and combination of antigens, antibodies raised against this genetically engineered P30 antigen and combination of antigens, as well as kits and vaccines containing the genetically engineered P30 antigen and antigens present in the combination.
摘要翻译：本发明涉及可用于检测弓形虫的IgM和/或IgG抗体的遗传工程化的P30抗原和抗原（例如，遗传工程化的P30抗原和P35）的组合或混合物。此外，本发明还涉及使用这种遗传工程化的P30抗原和抗原组合，针对该遗传工程化的P30抗原产生的抗体和抗原组合的方法，以及含有遗传工程化的P30抗原的试剂盒和疫苗以及存在于组合。

7. 发明授权

US07432046B2 Methods for the determination of antibody IgG avidity 有权
标题翻译：测定抗体IgG亲合力的方法
公开(公告)号：US07432046B2
公开(公告)日：2008-10-07
申请号：US11265481
申请日：2005-11-02
申请人： Gregory T. Maine , Stephen C. Hsu , Darwin D. Smith , Dominick L. Pucci , Jörg Herzogenrath , Ingo Curdt , Heike Maria Christ
发明人： Gregory T. Maine , Stephen C. Hsu , Darwin D. Smith , Dominick L. Pucci , Jörg Herzogenrath , Ingo Curdt , Heike Maria Christ
IPC分类号： G01N33/543 , G01N33/553 , G01N33/552 , G01N33/544 , G01N33/546 , G01N33/53 , G01N33/00 , C12Q1/70
CPC分类号： G01N33/56905 , G01N33/56994 , G01N33/6854 , G01N2333/45
摘要： The present invention relates to methods of determining anti-infectious agent IgG avidity, for example, human anti-cytomegalovirus and human anti-toxoplasma IgG avidity, using a competitive assay format.
摘要翻译：本发明涉及使用竞争性测定形式测定抗感染剂IgG亲合力的方法，例如人抗巨细胞病毒和人抗弓形体IgG亲合力。

8. 发明授权

US06287760B1 Western blot test for the identification of antibodies specific against the HCMV virus 有权
标题翻译：用于鉴定针对HCMV病毒的抗体的蛋白质印迹测试
公开(公告)号：US06287760B1
公开(公告)日：2001-09-11
申请号：US09242391
申请日：1999-07-08
申请人： Maria P. Landini , Gregory T. Maine , Alessandro Ripalti , Tiziana Lazzarotto
发明人： Maria P. Landini , Gregory T. Maine , Alessandro Ripalti , Tiziana Lazzarotto
IPC分类号： C12Q170
CPC分类号： G01N33/56994 , C07K14/005 , C07K2319/00 , C12N2710/16122 , G01N33/543 , G01N33/54386 , G01N33/559 , G01N2333/045 , G01N2469/20
摘要： Diagnostic tool for the identification of anti-HCMV antibodies in human serum. The tool comprises a means of solid support having two sections, a first section bearing a plurality of HCMV proteins (vp) obtained from purified virions concentrated in separate bands forming a predetermined pattern, one of these bands being pp 150 of HCMV; a second section bearing recombinant fusion proteins as controls, at least one band comprising a recombinant fusion protein carrying at least one epitope of pp 150.
摘要翻译：用于鉴定人血清中抗HCMV抗体的诊断工具。该工具包括具有两个部分的固体支持装置，第一部分带有多个HCMV蛋白（vp），其从浓缩在形成预定图案的分离带中的纯化病毒体获得，这些条带中的一个是HCMV的pp 150; 第二部分，其携带重组融合蛋白作为对照，至少一个带包含携带至少一个pp 150的表位的重组融合蛋白。

9. 发明申请

US20080160606A1 GENETICALLY ENGINEERED P30 ANTIGEN, IMPROVED ANTIGEN COCKTAIL, AND USES THEREOF 有权
标题翻译：遗传工程P30抗原，改良的抗原蛋白及其用途
公开(公告)号：US20080160606A1
公开(公告)日：2008-07-03
申请号：US11860383
申请日：2007-09-24
申请人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
发明人： Gregory T. Maine , Chandu B. Patel , Sanford R. Ginsburg , Timothy R. Bliese
IPC分类号： C12N15/00 , C07H21/04
CPC分类号： C07K14/45 , A61K39/00 , G01N33/56905 , G01N2333/45 , G01N2469/20 , Y10S435/975
摘要： The present invention relates to a genetically engineered P30 antigen and a combination or mixture of antigens (e.g., the genetically engineered P30 antigen and P35) that may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using this genetically engineered P30 antigen and combination of antigens, antibodies raised against this genetically engineered P30 antigen and combination of antigens, as well as kits and vaccines containing the genetically engineered P30 antigen and antigens present in the combination.
摘要翻译：本发明涉及可用于检测弓形虫的IgM和/或IgG抗体的遗传工程化的P30抗原和抗原（例如，遗传工程化的P30抗原和P35）的组合或混合物。此外，本发明还涉及使用这种遗传工程化的P30抗原和抗原组合，针对该遗传工程化的P30抗原产生的抗体和抗原组合的方法，以及含有遗传工程化的P30抗原的试剂盒和疫苗以及存在于组合。

10. 发明授权

US06177241B1 Use of peptides to improve specificity of an immunoassay for the detection of cytomegalovirus specific IgM antibody 失效
标题翻译：使用肽来提高检测巨细胞病毒特异性IgM抗体的免疫测定的特异性
公开(公告)号：US06177241B1
公开(公告)日：2001-01-23
申请号：US08935009
申请日：1997-09-22
申请人： Gregory T. Maine
发明人： Gregory T. Maine
IPC分类号： C12Q170
CPC分类号： G01N33/56994
摘要： This invention provides a method, a reagent, and a kit for detecting herpesvirus-specific IgM antibodies indicative of recent infection while preventing detection of low levels of herpesvirus-specific IgM antibodies present in individuals of low risk. The invention also provides a reagent for use in detecting herpesvirus-specific IgM antibodies indicative of recent infection while preventing detection of low levels of herpesvirus-specific IgM antibodies present in individuals of low risk. The method of this invention comprises the steps of: (a) providing a solid phase containing at least a portion of a herpesvirus antigen; (b) introducing a patient specimen to the solid phase of step (a); (c) introducing at least one peptide capable of specifically binding IgM antibodies to herpesvirus to the solid phase of step (a); d) allowing IgM antibodies to herpesvirus to specifically bind to the portion of antigen of herpesvirus and the peptide capable of specifically binding IgM antibodies to herpesvirus to the solid phase; and (e) determining the level of IgM antibodies to herpesvirus in the patient specimen.
摘要翻译：本发明提供了一种用于检测指示最近感染的疱疹病毒特异性IgM抗体的方法，试剂和试剂盒，同时防止存在于低风险个体中的低水平疱疹病毒特异性IgM抗体。本发明还提供了用于检测指示最近感染的疱疹病毒特异性IgM抗体的试剂，同时防止检测低风险个体中存在的低水平疱疹病毒特异性IgM抗体。本发明的方法包括以下步骤：（a ）提供含有至少一部分疱疹病毒抗原的固相;（b）将患者标本导入步骤（a）的固相;（c）将至少一种能够将IgM抗体特异性结合疱疹病毒的肽引入到步骤（a）的固相; d）允许疱疹病毒的IgM抗体特异性结合疱疹病毒抗原部分和能够将IgM抗体与疱疹病毒特异性结合至固相的肽; 和（e）确定患者标本中疱疹病毒IgM抗体的水平。

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