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    • 5. 发明申请
    • METHOD AND DEVICE FOR PRODUCING AND/OR PURIFYING POLYNUCLEOTIDES AND PRODUCTS OBTAINABLE THEREOF
    • 用于生产和/或纯化多核苷酸的方法和装置及其可获得的产品
    • US20140342449A1
    • 2014-11-20
    • US14451951
    • 2014-08-05
    • EUROGENTEC S.A.
    • Philippe LEDENT
    • C12Q1/68
    • C12Q1/6806C07B63/00C12N15/1003
    • An apparatus and a method for obtaining a (poly)nucleotide sequence of interest include steps of cultivating hosts cells to produce a nucleotide sequence of interest and harvesting these cells, introducing these cells in a passageway and disintegrating them in a continuous process. In the continuous process, performing in the passageway a precipitation of contaminants by a mixing of the disintegrated cells with a solution containing one or more salt(s) and obtaining a mixture and allowing a precipitate to separate from the solution of this mixture, preferably to float and/or to sediment from the solution of this mixture for 1-48 hours and pumping out a soluble material from this solution, while excluding recovering the precipitate.
    • 获得感兴趣的(多)核苷酸序列的装置和方法包括培养宿主细胞以产生感兴趣的核苷酸序列并收获这些细胞的步骤,将这些细胞引入通道并在连续过程中分解它们。 在连续方法中,在通道中通过将崩解的细胞与含有一种或多种盐的溶液混合并获得混合物并使沉淀物与该混合物的溶液分离,在通道中沉淀污染物,优选为 从该混合物的溶液中漂浮和/或沉淀1-48小时,并从该溶液中泵出可溶性物质,同时排除回收沉淀物。
    • 9. 发明授权
    • Method and device for producing and/or purifying polynucleotides and products obtainable thereof
    • 用于生产和/或纯化可获得的多核苷酸和产物的方法和装置
    • US09416400B2
    • 2016-08-16
    • US14451951
    • 2014-08-05
    • EUROGENTEC S.A.
    • Philippe Ledent
    • C12M1/00C12M3/00C12Q1/68C07B63/00C12N15/10
    • C12Q1/6806C07B63/00C12N15/1003
    • An apparatus and a method for obtaining a (poly)nucleotide sequence of interest include steps of cultivating hosts cells to produce a nucleotide sequence of interest and harvesting these cells, introducing these cells in a passageway and disintegrating them in a continuous process. In the continuous process, performing in the passageway a precipitation of contaminants by a mixing of the disintegrated cells with a solution containing one or more salt(s) and obtaining a mixture and allowing a precipitate to separate from the solution of this mixture, preferably to float and/or to sediment from the solution of this mixture for 1-48 hours and pumping out a soluble material from this solution, while excluding recovering the precipitate.
    • 获得感兴趣的(多)核苷酸序列的装置和方法包括培养宿主细胞以产生感兴趣的核苷酸序列并收获这些细胞的步骤,将这些细胞引入通道并在连续过程中分解它们。 在连续方法中,在通道中通过将崩解的细胞与含有一种或多种盐的溶液混合并获得混合物并使沉淀物与该混合物的溶液分离,在通道中沉淀污染物,优选至 从该混合物的溶液中漂浮和/或沉淀1-48小时,并从该溶液中泵出可溶性物质,同时排除回收沉淀物。