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1. 发明申请

WO1995017519A1 IMPROVED THREE REAGENT GRAM STAINING METHOD AND KIT 审中-公开
标题翻译：改进的三种试剂染色方法和工具包
公开(公告)号：WO1995017519A1
公开(公告)日：1995-06-29
申请号：PCT/US1994011503
申请日：1994-10-11
申请人： DIFCO LABORATORIES , MESZAROS, Amy, Taylor , STRENKOSKI, Leon, F.
发明人： DIFCO LABORATORIES
IPC分类号： C12Q01/04
CPC分类号： C12Q1/04 , Y10S435/81
摘要： A method for Gram staining a specimen containing bacteria includes the steps of staining Gram positive bacteria in the specimen, fixing the stain to the Gram positive bacteria, and simultaneously decolorizing and counter-staining Gram negative bacteria in the specimen.
摘要翻译：对含有细菌的样品进行革兰氏染色的方法包括在样品中染色革兰氏阳性菌，将染色剂固定在革兰氏阳性细菌上，同时对样品中的革兰氏阴性细菌进行脱色和反染色的步骤。

2. 发明申请

WO1995021241A1 MICROBIOLOGICAL CULTURE BOTTLE, AND METHOD OF MAKING AND USING SAME 审中-公开
标题翻译：微生物培养瓶及其制造和使用方法
公开(公告)号：WO1995021241A1
公开(公告)日：1995-08-10
申请号：PCT/US1995001269
申请日：1995-01-30
申请人： DIFCO LABORATORIES
发明人： DIFCO LABORATORIES , EDEN, Ruth , EDEN, Gideon , McMILLIAN, Ray
IPC分类号： C12M01/00
CPC分类号： C12M23/08 , C12Q1/04
摘要： A container (10) adapted for use in the detection of aerobic microorganisms in a sample includes a non-toxic insert (22) disposed within the container (10) for supporting microorganisms adhered thereto and for increasing microbial exposure to oxygenated growth media to enhance microbial metabolism. A method for making the container (10) includes the steps of inserting a non-toxic insert (22) into the container (10) and adding growth media (14). Also, a method of detecting aerobic microbiological growth in a sealed sample container (10) having a headspace (16) and which contains a sample which may contain an unknown microorganism includes the steps of providing a sealed sample container (10) having a headspace (16) and non-toxic insert (22) saturated with microbiological growth media (24), inoculating the insert (22) within the sealed sample container (10), and monitoring metabolism in the container (10) as an indicator of the presence of microorganisms to detect microorganisms in the sample.
摘要翻译：适用于检测样品中需氧微生物的容器（10）包括：设置在容器（10）内的无毒插入物（22），用于支持附着于其上的微生物并增加微生物暴露于含氧生长培养基以增强微生物代谢。制造容器（10）的方法包括将无毒插入物（22）插入容器（10）并添加生长培养基（14）的步骤。此外，在具有顶部空间（16）并且含有可能含有未知微生物的样品的密封样品容器（10）中检测需氧微生物生长的方法包括以下步骤：提供具有顶部空间的密封样品容器（10） 16）和用微生物生长培养基（24）饱和的无毒插入物（22），将插入物（22）接种在密封的样品容器（10）内，并监测容器（10）中的代谢作为微生物检测样品中的微生物。

3. 发明申请

WO1993018182A1 DIAGNOSTIC MICROBIOLOGICAL TESTING APPARATUS AND METHOD 审中-公开
标题翻译：诊断微生物检测装置及方法
公开(公告)号：WO1993018182A1
公开(公告)日：1993-09-16
申请号：PCT/US1993002101
申请日：1993-03-08
申请人： DIFCO LABORATORIES
发明人： DIFCO LABORATORIES , EDEN, Gideon
IPC分类号： C12Q01/06
CPC分类号： C12M41/46 , G01N21/253 , G01N21/6428 , G01N2035/00118 , G01N2035/00148 , G01N2201/0415 , Y10S435/808 , Y10S435/809 , Y10S436/807 , Y10T436/111666 , Y10T436/112499
摘要： A diagnostic microbiological testing apparatus and method includes at least one test tray (11) including a plurality of reaction chambers (12), a light source (22) disposed proximate to the test tray (11) for directing light, at an excitation wavelength of a fluorescence emitting agent contained within the reaction chambers (12), at the test tray (11), a filter for passing therethrough only light generated by a fluorescence emitting reaction resulting from the interaction of the fluorescence emitting agent and a sample, and an imaging mechanism (26) for detecting only the light generated by the fluorescence emitting reaction at the emission wavelength simultaneously from the plurality of reaction chambers (12).
摘要翻译：一种诊断微生物测试装置和方法包括至少一个包括多个反应室（12）的测试托盘（11），设置在靠近测试托盘（11）的光源（22），用于引导光，激发波长为包含在反应室（12）内的荧光发射剂，在测试盘（11）处，仅通过由荧光发射剂和样品的相互作用产生的荧光发射反应产生的光的滤光器和成像机构（26），用于仅从多个反应室（12）同时检测由发射波长的荧光发射反应产生的光。

4. 发明公开

EP0743979A1 MICROBIOLOGICAL CULTURE BOTTLE, AND METHOD OF MAKING AND USING SAME 失效
标题翻译：微生物培养瓶，你的Herstelling和使用
公开(公告)号：EP0743979A1
公开(公告)日：1996-11-27
申请号：EP95909409.0
申请日：1995-01-30
申请人： DIFCO LABORATORIES
发明人： EDEN, Ruth , EDEN, Gideon , McMILLIAN, Ray
IPC分类号： C12M1 , C12Q1
CPC分类号： C12M23/08 , C12Q1/04
摘要： A container (10) adapted for use in the detection of aerobic microorganisms in a sample includes a non-toxic insert (22) disposed within the container (10) for supporting microorganisms adhered thereto and for increasing microbial exposure to oxygenated growth media to enhance microbial metabolism. A method for making the container (10) includes the steps of inserting a non-toxic insert (22) into the container (10) and adding growth media (14). Also, a method of detecting aerobic microbiological growth in a sealed sample container (10) having a headspace (16) and which contains a sample which may contain an unknown microorganism includes the steps of providing a sealed sample container (10) having a headspace (16) and non-toxic insert (22) saturated with microbiological growth media (24), inoculating the insert (22) within the sealed sample container (10), and monitoring metabolism in the container (10) as an indicator of the presence of microorganisms to detect microorganisms in the sample.

5. 发明授权

EP0558827B1 Process and kit for detecting microbial metabolism 失效
标题翻译：方法和试剂盒，用于检测微生物的代谢
公开(公告)号：EP0558827B1
公开(公告)日：1996-10-30
申请号：EP92200617.6
申请日：1992-03-03
申请人： DIFCO LABORATORIES
发明人： Thompson, Thomas E. , Eden, Ruth F.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q2304/20 , Y10S436/80

6. 发明公开

EP0558827A1 Process and kit for detecting microbial metabolism 失效
标题翻译： Verfahren und Kit zum Nachweis von mikrobiellen代谢。
公开(公告)号：EP0558827A1
公开(公告)日：1993-09-08
申请号：EP92200617.6
申请日：1992-03-03
申请人： DIFCO LABORATORIES
发明人： Thompson, Thomas E. , Eden, Ruth F.
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q2304/20 , Y10S436/80
摘要： A process and kit for detecting microbial metabolism is disclosed, the process including the steps of combining medium, a metabolic dye having an absorbance spectrum which in the presence of microorganisms changes and responds to metabolism of the microorganism and a second analytical dye having an excitation or emission spectrum which overlaps with one of the changed or unchanged absorbance spectra of the first dye. The medium contains a sample solution to be analyzed for containing metabolizing microorganisms. The user observes either no change in the fluorescence emissions of the second analytical dye indicating the absence of metabolizing microorganisms in the sample or a change indicating the presence of metabolizing microorganisms in the sample.
摘要翻译：公开了一种用于检测微生物代谢的方法和试剂盒，该方法包括将培养基，具有在微生物存在下的吸收光谱变化并对微生物代谢作出反应的代谢染料和具有激发的第二分析染料的步骤，发射光谱与第一染料的改变或改变的吸收光谱之一重叠。培养基含有待分析的含有代谢微生物的样品溶液。用户观察到第二种分析染料的荧光发射没有变化，表明样品中没有代谢微生物，或者表明样品中存在代谢微生物的变化。

7. 发明公开

EP0329120A3 Luminescence test and exposure apparatus 失效
标题翻译：发光测试和曝光装置
公开(公告)号：EP0329120A3
公开(公告)日：1991-10-02
申请号：EP89102616.3
申请日：1989-02-16
申请人： RMS LABORATORIES, INC. , DIFCO LABORATORIES
发明人： Wannlund, Jon C. , Smith, Jerry W.
IPC分类号： G01N21/76 , G01N33/493 , C12M1/18
CPC分类号： B01L3/5025 , B01L3/50255 , G01N21/03 , G01N21/76 , G01N2021/0325 , G01N2021/0328 , G01N2021/0357 , G01N2021/1768 , G01N2201/0446 , Y10T436/111666 , Y10T436/25375
摘要： Apparatus for performing and measuring chemical reactions includes a reaction test apparatus having reaction wells wherein reactants are controllably mixed, and exposure apparatus which receives and positions the reaction test apparatus adjacent a photographic film. Each of the reaction wells includes at least two reaction chambers, arranged in a side by side fashion. All but the final reaction chamber have upwardly sloping sides, so that liquid placed in one reaction chamber can flow to the next reaction chamber when the apparatus is tilted. In a preferred embodiment, the reaction wells are supported in a plate that is structurally integral with the wells but separates the wells from each other. The test plate is retained in the exposure apparatus, and liquid is controllably flowed from one reaction chamber to the next by tilting the exposure apparatus. The apparatus of the invention is particularly suited for measuring reactions that produce luminescence of short duration, as the reactants can be conveniently mixed in darkness, while the film is being exposed.

8. 发明公开

EP0401660A1 Culture media package 失效
标题翻译： Kulturmedium-VERPACKUNG。
公开(公告)号：EP0401660A1
公开(公告)日：1990-12-12
申请号：EP90110247.5
申请日：1990-05-30
申请人： DIFCO LABORATORIES INCORPORATED
发明人： Smith, Jerry W. , Sullivan, Nadine M.
IPC分类号： C12M1/22
CPC分类号： C12M23/38 , C12M23/10 , C12M23/34 , C12M37/04
摘要： A culture media package comprising a container (30) having a bottom wall (31) and a peripheral side wall (32) which is substantially filled with a culture medium, a removable insert (36) over the top wall and having a portion defining a planar undersurface and a removable protective cover (41) over the container and insert. The container and insert have radially extending flanges (33, 40) which are bonded to one another. The package is made by filling the container with a molten culture medium in a sterile environment, bonding the insert to the container in a sterile environment, inverting the sealed container such that the molten medium contacts the planar surface of the insert permitting any gases and bubbles to rise toward a space adjacent the bottom of the container and maintaining the container with the insert thereon in an inverted position until the medium has solidified. The removable cover may be applied to the sealed package of the container and insert either before the sealed package is inverted or after the medium has solidified. The package is preferably made from continuous molded plastic strips of connected containers and connected inserts which are filled with medium and sealed, severed from one another, thereafter inverted while the culture medium is still molten and permitted to remain in the inverted position until the culture medium has solidified.
摘要翻译：一种培养基包装，包括具有底壁（31）和基本上填充有培养基的周边侧壁（32）的容器（30），位于顶壁上方的可移除插入物（36）平面的下表面和容器上的可移除的保护盖（41）和插入物。容器和插入件具有彼此结合的径向延伸的凸缘（33,40）。包装是通过在无菌环境中用熔融培养基填充容器来制造的，在无菌环境中将插入物粘合到容器上，使密封容器翻转，使得熔融介质接触插入物的平坦表面，允许任何气体和气泡朝向靠近容器底部的空间上升，并将具有插入件的容器保持在倒置位置，直到介质凝固。可移除的盖可以被施加到容器的密封包装，并且在密封包装被倒置之前或在介质固化之后插入。该包装件优选由连续的容器和连接的插入物连续模制的塑料条制成，其中填充有介质并密封，彼此切断，之后倒置，同时培养基仍然熔化并允许保持在倒置位置，直到培养基已经凝固

9. 发明授权

EP0743979B1 MICROBIOLOGICAL CULTURE BOTTLE, AND METHOD OF MAKING AND USING SAME 失效
标题翻译：微生物培养瓶，你的Herstelling和使用
公开(公告)号：EP0743979B1
公开(公告)日：2003-05-02
申请号：EP95909409.5
申请日：1995-01-30
申请人： DIFCO LABORATORIES
发明人： EDEN, Ruth , EDEN, Gideon , McMILLIAN, Ray
IPC分类号： C12M1/00 , C12Q1/02
CPC分类号： C12M23/08 , C12Q1/04
摘要： A container (10) adapted for use in the detection of aerobic microorganisms in a sample includes a non-toxic insert (22) disposed within the container (10) for supporting microorganisms adhered thereto and for increasing microbial exposure to oxygenated growth media to enhance microbial metabolism. A method for making the container (10) includes the steps of inserting a non-toxic insert (22) into the container (10) and adding growth media (14). Also, a method of detecting aerobic microbiological growth in a sealed sample container (10) having a headspace (16) and which contains a sample which may contain an unknown microorganism includes the steps of providing a sealed sample container (10) having a headspace (16) and non-toxic insert (22) saturated with microbiological growth media (24), inoculating the insert (22) within the sealed sample container (10), and monitoring metabolism in the container (10) as an indicator of the presence of microorganisms to detect microorganisms in the sample.

10. 发明公开

EP0447893A2 Culture dish package system and method of making 失效
标题翻译：系统与Verfahren zum Verpacken von Petrischalen。
公开(公告)号：EP0447893A2
公开(公告)日：1991-09-25
申请号：EP91103558.2
申请日：1991-03-08
申请人： DIFCO LABORATORIES INCORPORATED
发明人： Smith, Jerry W. , Sullivan, Nadine M. , Eden, Ruth F.
IPC分类号： C12M1/22
CPC分类号： C12M37/00 , C12M23/10
摘要： A culture package system comprising a package (10) preferably in the form of a container containing one or more conventional culture media dishes (12) containing solidified culture media. Each dish (12) includes a bottom wall and a peripheral side wall and a removable cover (14) having a bottom wall and a peripheral side wall which is loosely telescoped over the dish (12). A plurality of the dishes (12) are preferably provided in inverted staked relation in the container (10). The interior of the package (10) has a gaseous atmosphere having less than 1% oxygen. An oxygen absorber (18) is placed in the package (10) to facilitate maintaining the atmosphere at less than 1% oxygen. Preferably a moisture absorber (20) is also placed in the package (10). The package (10) is made of plastic material which is impermeable to oxygen and moisture and preferably is in the form of a container which has a base wall and a peripheral wall and a cover (16) sealed thereto.
摘要翻译：一种培养包装系统，其包含优选为容器形式的包装（10），所述容器包含含有固化培养基的一种或多种常规培养基培养皿（12）。每个盘（12）包括底壁和周边侧壁以及可拆卸盖（14），其具有松散地套在盘（12）上的底壁和周边侧壁。多个餐具（12）优选地以倒置的关系提供在容器（10）中。包装（10）的内部具有小于1％的氧的气体气氛。将氧吸收器（18）放置在包装（10）中，以便于将气氛维持在小于1％的氧气。优选地，吸湿剂（20）也被放置在包装（10）中。包装（10）由对氧气和水分不透气的塑料制成，优选为具有底壁和周壁的容器形式，以及密封于其上的盖（16）。

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