专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

GB9117998D0 ODOUR ADSORBING MEANS 未知
公开(公告)号：GB9117998D0
公开(公告)日：1991-10-09
申请号：GB9117998
申请日：1991-08-21
申请人： WILLIAMS GLANMOR T , DAVIS RONALD W
IPC分类号： A61F5/441 , A61L9/01 , A61L15/18 , A61L28/00

2. 发明申请

WO2007008246A3 CHARGE PERTURBATION DETECTION SYSTEM FOR DNA AND OTHER MOLECULES 审中-公开
标题翻译：用于DNA和其他分子的电荷刺激检测系统
公开(公告)号：WO2007008246A3
公开(公告)日：2008-03-06
申请号：PCT/US2005040558
申请日：2005-11-10
申请人： UNIV LELAND STANFORD JUNIOR , POURMAND NADER , KARHANEK MILOSLAV , DAVIS RONALD W
发明人： POURMAND NADER , KARHANEK MILOSLAV , DAVIS RONALD W
IPC分类号： C12M1/34 , C12N11/16 , G01N15/06
CPC分类号： C12Q1/6825 , B82Y15/00 , B82Y30/00 , B82Y40/00 , G01N27/3275 , G01N27/3276
摘要： Methods and apparatus for direct detection of chemical reactions are provided. In a preferred embodiment, electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The target molecule is preferably DNA. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time.
摘要翻译：提供了用于直接检测化学反应的方法和装置。在优选的实施方案中，酶催化反应期间局部环境的电荷扰动由具有固定化靶分子的电极系统检测。靶分子优选为DNA。由聚合酶反应引起的电荷扰动可以唯一地识别DNA序列。聚合过程在电极表面附近的溶液中产生电荷的局部扰动，并在极化金电极中引起电荷。该事件由电压钳位放大器检测为瞬态电流。可以通过将单个dNTP分配到电极溶液并检测电荷扰动来确定序列中单个核苷酸的检测。或者，可以同时确定多个碱基。

3. 发明申请

WO2006034349A2 DNA FINGERPRINTING USING A BRANCH MIGRATION ASSAY 审中-公开
标题翻译：使用分支迁移测定的DNA指纹印记
公开(公告)号：WO2006034349A2
公开(公告)日：2006-03-30
申请号：PCT/US2005/033838
申请日：2005-09-21
申请人： THE BOARD OF TRUSTEES OF THE LELAND STANDFORD JUNIOR UNIVERSITY , POURMAND, Nader , DAVIS, Ronald, W. , WANG, Shan, X.
发明人： POURMAND, Nader , DAVIS, Ronald, W. , WANG, Shan, X.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/6837 , C12Q2565/519 , C12Q2537/1373 , C12Q2525/204
摘要： A method of determining the length of a polynucleotide target is provided. With this method, a target is first hybridized to an array of first probes having different, determined lengths, resulting in the formation of duplexes between the polynucleotide target and the first probes. These duplexes have a single stranded section of target if the target is longer than the first probe it is in a duplex with. Next, a second probe having a determined length is hybridized to these duplexes. If the length of the target is greater than the length of the first probe it is displaced during this hybridization step by the process of branch migration. In contrast, if the length of the target is less than or equal to the length of the first probe, it is not displaced. Thus, the length of the polynucleotide target can be determined.
摘要翻译：提供了确定多核苷酸靶标长度的方法。利用该方法，首先将靶标与具有不同确定长度的第一探针阵列杂交，导致多核苷酸靶标与第一探针之间形成双链体。如果目标长度大于第一个探针与双工目标的距离，则这些双链具有单链目标部分。接下来，具有确定长度的第二探针与这些双链体杂交。如果目标的长度大于第一个探针的长度，则在该杂交步骤期间通过分支迁移的过程而被移位。相反，如果目标的长度小于或等于第一个探针的长度，则不会发生位移。因此，可以确定多核苷酸靶标的长度。

4. 发明申请

WO2005123963A2 METHODS AND COMPOSITIONS FOR USE IN ANALYTE DETECTION USING PROXIMITY PROBES 审中-公开
标题翻译：在分析检测中使用临近探针的方法和组合
公开(公告)号：WO2005123963A2
公开(公告)日：2005-12-29
申请号：PCT/US2005021058
申请日：2005-06-14
申请人： UNIV LELAND STANFORD JUNIOR , FREDRIKSSON SIMON , DAVIS RONALD W
发明人： FREDRIKSSON SIMON , DAVIS RONALD W
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6804 , G01N33/542 , C12Q2563/179 , C12Q2522/101 , C12Q2561/125 , C12Q2537/162 , C12Q2537/125
摘要： Methods and compositions for detecting an analyte in a sample are provided. In practicing the subject methods, a sample is combined with at least a pair of proximity probes that each include an analyte binding domain and a nucleic acid domain. The resultant mixture is then contacted with a pair of asymmetric nucleic acid connectors. Proximity dependent connector mediated interaction between the nucleic acid domains of the proximity probes is then detected to determine the presence of the analyte in the sample. Also provided are kits and systems for practicing the subject methods.
摘要翻译：提供了用于检测样品中分析物的方法和组合物。在实施本发明方法时，将样品与至少一对邻近探针组合，每个邻近探针包括分析物结合结构域和核酸结构域。然后将所得混合物与一对不对称核酸连接体接触。然后检测接近探针的核酸结构域之间的邻近依赖连接体介导的相互作用，以确定样品中分析物的存在。还提供了用于实践主题方法的套件和系统。

5. 发明申请

WO2015112323A1 RE-USABLE ANALYTE DETECTOR AND METHODS 审中-公开
标题翻译：可重新分析检测器和方法
公开(公告)号：WO2015112323A1
公开(公告)日：2015-07-30
申请号：PCT/US2015/010161
申请日：2015-01-05
申请人： BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY , HARRIS, James, S., Jr. , DAVIS, Ronald, W. , ESFANDYARPOUR, Rahim
发明人： HARRIS, James, S., Jr. , DAVIS, Ronald, W. , ESFANDYARPOUR, Rahim
IPC分类号： G01N21/75 , G01N33/48 , G01N35/00 , G01N35/08
CPC分类号： G01N33/5438 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2300/0645 , B01L2300/0663 , B01L2300/0887 , B01L2300/12 , B01L2300/16 , B01L2400/043 , C12Q1/6869 , G01N33/54326
摘要： Devices and methods for detecting the presence of one or more analytes, such as biomolecules (Fig. 2). An analyte detection device includes at least two conductive structures disposed between three insulating structures and a gap exists in the structures that defines a channel such that at least four electrodes capable of measuring an electrical property are present in the channel. Methods for improving analyte detector performance through low-salt buffer washes also are disclosed.
摘要翻译：用于检测一种或多种分析物（例如生物分子）的存在的装置和方法（图2）。分析物检测装置包括设置在三个绝缘结构之间的至少两个导电结构，并且在限定通道的结构中存在间隙，使得能够测量电特性的至少四个电极存在于通道中。还公开了通过低盐缓冲液洗涤改善分析物检测器性能的方法。

6. 发明申请

WO2006034349A3 DNA FINGERPRINTING USING A BRANCH MIGRATION ASSAY 审中-公开
标题翻译：使用分支移动测定DNA指纹
公开(公告)号：WO2006034349A3
公开(公告)日：2006-08-24
申请号：PCT/US2005033838
申请日：2005-09-21
申请人： OR UNIVERSITY THE BOARD OF TRU , POURMAND NADER , DAVIS RONALD W , WANG SHAN X
发明人： POURMAND NADER , DAVIS RONALD W , WANG SHAN X
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/68 , C12Q1/6837 , C12Q2565/519 , C12Q2537/1373 , C12Q2525/204
摘要： A method of determining the length of a polynucleotide target is provided. With this method, a target is first hybridized to an array of first probes having different, determined lengths, resulting in the formation of duplexes between the polynucleotide target and the first probes. These duplexes have a single stranded section of target if the target is longer than the first probe it is in a duplex with. Next, a second probe having a determined length is hybridized to these duplexes. If the length of the target is greater than the length of the first probe it is displaced during this hybridization step by the process of branch migration. In contrast, if the length of the target is less than or equal to the length of the first probe, it is not displaced. Thus, the length of the polynucleotide target can be determined.
摘要翻译：提供了确定多核苷酸靶标长度的方法。利用该方法，首先将靶与具有不同的确定长度的第一探针阵列杂交，导致在多核苷酸靶和第一探针之间形成双链体。如果目标比第一个探针更长，那么这些双链体具有目标单链段。接下来，具有确定长度的第二探针与这些双链体杂交。如果靶的长度大于第一探针的长度，则在该杂交步骤期间通过分支迁移的过程被置换。相反，如果目标的长度小于或等于第一探针的长度，则其不被移位。因此，可以确定多核苷酸靶的长度。

7. 发明申请

WO0248174A3 DIMERIC FLUORESCENT POLYPEPTIDES 审中-公开
标题翻译：二聚体荧光多肽
公开(公告)号：WO0248174A3
公开(公告)日：2003-08-14
申请号：PCT/US0148690
申请日：2001-12-13
申请人： STRATAGENE INC , DAVIS RONALD W , VAILLANCOURT PETER
发明人： DAVIS RONALD W , VAILLANCOURT PETER
IPC分类号： C07K14/435 , G01N33/52 , C12N15/62
CPC分类号： C07K14/43595 , C07K2319/00
摘要： The invention relates to proteins or polypeptides that comprise intramolecular dimers of fluorescent protein monomers. More specifically, the invention relates t recombinant polypeptides comprising a monomer of a fluorescent polypeptide, a linker peptide, and a second monomer of that fluorescent polypeptide, where the monomers form an intramolecular dimer. The invention also relates to nucleic acids encoding Intramolicular Dimer Fluorescent Proteins (IDFPs) and vectors comprising such a nicleic acids. The invention further relates to methods of making IDFPs and methods of using them. IDFPs are useful in any application suited for fluorescent proteins and are particularly useful in applications in which more than one fluorescent protein sharing complementary dimerization interfaces is present in the same mixture or is expressed in the same cell, because IDFPs do not form heterodimers.
摘要翻译：本发明涉及包含荧光蛋白单体的分子内二聚体的蛋白或多肽。更具体地说，本发明涉及包含荧光多肽单体，接头肽和该荧光多肽的第二单体的重组多肽，其中单体形成分子内二聚体。本发明还涉及编码胞内二聚体荧光蛋白（IDFP）的核酸以及包含这种胞壁酸的载体。本发明还涉及制造IDFP的方法和使用它们的方法。 IDFP可用于任何适用于荧光蛋白的应用，尤其适用于共享互补二聚化界面的多于一种荧光蛋白存在于相同混合物中或在相同细胞中表达的应用，因为IDFP不形成异二聚体。

8. 发明申请

WO2009076560A2 METHOD AND APPARATUS FOR MAGNETIC SEPARATION OF CELLS 审中-公开
标题翻译：用于磁细胞分离的方法和装置
公开(公告)号：WO2009076560A2
公开(公告)日：2009-06-18
申请号：PCT/US2008/086486
申请日：2008-12-11
申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY , TALASAZ, Amirali, Hajhossein , POWELL, Ashley, Ann , PEASE, Fabian, R. , JEFFREY, Stefanie, S. , DAVIS, Ronald, W. , MINDRINOS, Michael
发明人： TALASAZ, Amirali, Hajhossein , POWELL, Ashley, Ann , PEASE, Fabian, R. , JEFFREY, Stefanie, S. , DAVIS, Ronald, W. , MINDRINOS, Michael
IPC分类号： C12M3/00 , G01N33/574
CPC分类号： G01N33/54333 , B03C1/01 , B03C1/286 , B03C2201/18 , B03C2201/26
摘要： Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.
摘要翻译：这里描述了一种自动化的机器人装置，其以非常高的纯度分离循环肿瘤细胞（CTC）或其他生物结构。该设备使用强大的磁棒覆盖在可拆卸的塑料袖子。这些棒穿过血液样品，捕获例如用与磁性响应颗粒例如超顺磁珠连接的抗体标记的癌细胞。在完成捕获方案后，磁棒经历几轮洗涤，从而除去所有污染的血细胞。通过从袖子中取出磁棒将捕获的靶细胞释放到最终的捕获溶液中。此外，由该装置捕获的细胞在捕获后培养时显示出没有降低的活力。以适合于遗传分析的状态捕获细胞。还公开了用于单细胞分析的方法。机器人允许设备以高吞吐量运行。

9. 发明申请

WO2007008246A2 CHARGE PERTURBATION DETECTION SYSTEM FOR DNA AND OTHER MOLECULES 审中-公开
标题翻译：用于DNA和其他分子的电荷刺激检测系统
公开(公告)号：WO2007008246A2
公开(公告)日：2007-01-18
申请号：PCT/US2005/040558
申请日：2005-11-10
申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY , POURMAND, Nader , KARHANEK, Miloslav , DAVIS, Ronald, W.
发明人： POURMAND, Nader , KARHANEK, Miloslav , DAVIS, Ronald, W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , B82Y15/00 , B82Y30/00 , B82Y40/00 , G01N27/3275 , G01N27/3276
摘要： Methods and apparatus for direct detection of chemical reactions are provided. In a preferred embodiment, electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The target molecule is preferably DNA. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time.
摘要翻译：提供了用于直接检测化学反应的方法和装置。在优选的实施方案中，酶催化反应期间局部环境的电荷扰动由具有固定化靶分子的电极系统检测。靶分子优选为DNA。由聚合酶反应引起的电荷扰动可以唯一地识别DNA序列。聚合过程在电极表面附近的溶液中产生电荷的局部扰动，并在极化金电极中引起电荷。该事件由电压钳位放大器检测为瞬态电流。可以通过将各个dNTP分配到电极溶液中并检测电荷扰动来确定序列中单个核苷酸的检测。或者，可以同时确定多个碱基。

10. 发明申请

WO0248174A9 DIMERIC FLUORESCENT POLYPEPTIDES 审中-公开
标题翻译：二聚氟聚合物
公开(公告)号：WO0248174A9
公开(公告)日：2003-04-24
申请号：PCT/US0148690
申请日：2001-12-13
申请人： STRATAGENE INC , DAVIS RONALD W , VAILLANCOURT PETER
发明人： DAVIS RONALD W , VAILLANCOURT PETER
IPC分类号： C07K14/435 , C07K
CPC分类号： C07K14/43595 , C07K2319/00
摘要： The invention relates to proteins or polypeptides that comprise intramolecular dimers of fluorescent protein monomers. More specifically, the invention relates t recombinant polypeptides comprising a monomer of a fluorescent polypeptide, a linker peptide, and a second monomer of that fluorescent polypeptide, where the monomers form an intramolecular dimer. The invention also relates to nucleic acids encoding Intramolicular Dimer Fluorescent Proteins (IDFPs) and vectors comprising such a nicleic acids. The invention further relates to methods of making IDFPs and methods of using them. IDFPs are useful in any application suited for fluorescent proteins and are particularly useful in applications in which more than one fluorescent protein sharing complementary dimerization interfaces is present in the same mixture or is expressed in the same cell, because IDFPs do not form heterodimers.
摘要翻译：本发明涉及包含荧光蛋白单体的分子内二聚体的蛋白质或多肽。更具体地，本发明涉及包含荧光多肽的单体，接头肽和该荧光多肽的第二单体的重组多肽，其中单体形成分子内二聚体。本发明还涉及编码胞内二聚体荧光蛋白（IDFP）的核酸和包含这种纳米酸的载体。本发明还涉及制造IDFP的方法和使用它们的方法。 IDFP可用于任何适用于荧光蛋白的应用，并且特别适用于多于一种共同互补二聚化界面的荧光蛋白共存在同一混合物中或由同一细胞表达的应用，因为IDFP不形成异二聚体。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式