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1. 发明申请

WO2013013318A1 TWO-STAGE CONTINUOUS PRE-TREATMENT OF LIGNOCELLULOSIC BIOMASS 审中-公开
标题翻译：两阶段连续预处理细菌生物量
公开(公告)号：WO2013013318A1
公开(公告)日：2013-01-31
申请号：PCT/CA2012/050496
申请日：2012-07-20
申请人： GREENFIELD ETHANOL INC. , DOTTORI, Frank, A. , BENSON, Robert, Ashley, Cooper , BENECH, Régis-Olivier
发明人： DOTTORI, Frank, A. , BENSON, Robert, Ashley, Cooper , BENECH, Régis-Olivier
IPC分类号： D21C1/02 , C12P19/00 , C12P7/10 , C13K1/02
CPC分类号： C08H8/00 , C08B37/0057 , Y02E50/16 , Y02E50/343
摘要： Disclosed is a method of pretreating biomass in two pretreatment stages as part of a biofuel production process. The first stage pretreatment is carried out by heating the biomass to a first stage temperature of 140°C to 180°C for a first stage time of 30 minutes to 2 hours at a first stage pressure of 105 to 150 psig; and the second stage is carried out by heating the biomass to a second stage temperature of 190°C to 210°C for a second stage time of 2 to 10 minutes at a second stage pressure of 167 to 262 psig. The biomass may be initially conditioned prior to the first pretreatment stage by atmospheric steam heating and adjusting the moisture content of the biomass. Hemicellulose and inhibitors (inhibitory compounds) to downstream hydrolysis and fermentation are preferably removed between the first and second pretreating stages, more preferably after each pretreatment stage.
摘要翻译：公开了在两个预处理阶段中预处理生物质的方法，作为生物燃料生产过程的一部分。第一阶段预处理通过在105至150psig的第一阶段压力下将生物质加热至140℃至180℃的第一阶段温度进行30分钟至2小时的第一阶段时间; 并且第二阶段通过在167-262psig的第二阶段压力下将生物质加热至190℃至210℃的第二阶段温度进行2至10分钟的第二阶段时间。生物质可以在第一预处理阶段之前通过大气蒸汽加热和调节生物量的水分含量来进行初始调节。优选在第一和第二预处理阶段之间去除下游水解和发酵的半纤维素和抑制剂（抑制化合物），更优选在每个预处理阶段之后。

2. 发明申请

WO2012061939A1 CONTINUOUS PROCESS FOR THE PRODUCTION OF ETHANOL FROM LIGNOCELLULOSIC BIOMASS 审中-公开
标题翻译：从木质纤维素生物质生产乙醇的连续方法
公开(公告)号：WO2012061939A1
公开(公告)日：2012-05-18
申请号：PCT/CA2011/050692
申请日：2011-11-08
申请人： GREENFIELD ETHANOL INC. , DOTTORI, Frank, A. , BENSON, Robert, Ashley, Cooper , BENECH, Régis-olivier
发明人： DOTTORI, Frank, A. , BENSON, Robert, Ashley, Cooper , BENECH, Régis-olivier
IPC分类号： C12P7/10 , C08H8/00 , C12P19/00
CPC分类号： C12P7/10 , Y02E50/16
摘要： A continuous process for the recovery of ethanol from hemicellulose and cellulose from lignocellulosic biomass. Yield of fermentable sugars can be maximized by continuous operation of the pre-treatment system and careful selection of pretreatment conditions including the addition of only small amounts of dilute mineral acid and low pressure. With this approach, the xylose component that is mainly present in its unfermentable oligomeric form in known pre-hydrolysis Kraft processes can be recovered more efficiently and as a monomer that can be fermented by xylose fermenting yeasts and bacteria. Due to the use of only dilute acids, there is a very low loss of glucose and xylose hence very low production of toxic chemicals (e.g. HMF, furfural) in the pretreatment step. The resulting overall fermentation efficiency of both hexose and pentose sugars is 90 % of the theoretical maximum.
摘要翻译：从木质纤维素生物质的半纤维素和纤维素中回收乙醇的连续方法。通过预处理系统的连续操作和仔细选择预处理条件，包括仅添加少量稀无机酸和低压，可以使可发酵糖的产量最大化。通过这种方法，主要以其已知的预水解牛皮纸法以其不可发酵的低聚物形式存在的木糖组分可以更有效地回收，并作为可以通过木糖发酵酵母和细菌发酵的单体。由于仅使用稀酸，因此在预处理步骤中葡萄糖和木糖的损失非常低，因此非常低的有毒化学品（例如HMF，糠醛）的生产。得到的己糖和戊糖的整体发酵效率是理论最大值的90％。

3. 发明申请

WO2010071987A1 USE OF STILLAGE RESIDUE AS A NUTRIENT SOURCE FOR FERMENTATION OF HYDROLYZED LIGNOCELLULOSIC BIOMASS TO ETHANOL 审中-公开
标题翻译：作为用于发酵水解生物质的乙醇的营养源的使用
公开(公告)号：WO2010071987A1
公开(公告)日：2010-07-01
申请号：PCT/CA2009/001844
申请日：2009-12-22
申请人： GREENFIELD ETHANOL INC. , BENSON, Robert, Ashley, Cooper , BENECH , Regis -Olivier
发明人： BENSON, Robert, Ashley, Cooper , BENECH , Regis -Olivier
IPC分类号： C12P7/10 , C08B1/00 , C08H8/00 , C12P7/06 , C12P19/14
CPC分类号： C12P7/10 , Y02E50/16 , Y02E50/17
摘要： A process for the production of ethanol wherein a hydrolyzed lignocellulosic biomass is fermented in the presence of a stillage residue. The fermentation of cellulosic hydrolysates is improved by adding prior to and/or during fermentation a stillage residue side stream from a corn starch-to-ethanol process as a nutrient source for the yeast organisms used in the fermentation. Stillage residues from the grain dry mill ethanol producing process, including the whole stillage, wet cake, thin stillage, and/or syrup are added to assist as a nitrogen and nutrient source for the fermentive processes. The stillage residue is produced by any grain-to- ethanol process.
摘要翻译：一种生产乙醇的方法，其中水解的木质纤维素生物质在釜馏残渣存在下被发酵。纤维素水解产物的发酵通过在发酵前和/或发酵期间添加作为发酵中使用的酵母生物的营养源的玉米淀粉 - 乙醇方法的釜渣残留物流进行改进。添加来自谷物干磨乙醇生产过程的残留物，包括整个釜渣，湿饼，薄釜渣和/或糖浆，以辅助作为发酵过程的氮和营养源。酒糟残留物是通过任何谷物 - 乙醇方法生产的。

4. 发明申请

WO2013010271A1 CONDITIONING OF BIOMASS FOR IMPROVED C5/C6 SUGAR RELEASE PRIOR TO FERMENTATION 审中-公开
标题翻译：生物量调节改善C5 / C6蔗糖发酵前的发酵
公开(公告)号：WO2013010271A1
公开(公告)日：2013-01-24
申请号：PCT/CA2012/050481
申请日：2012-07-12
申请人： GREENFIELD ETHANOL INC. , DOTTORI, Frank, A. , BENSON, Robert, Ashley, Cooper , BENECH, Régis-Olivier
发明人： DOTTORI, Frank, A. , BENSON, Robert, Ashley, Cooper , BENECH, Régis-Olivier
IPC分类号： C13K13/00 , C10L1/182 , C13K1/02 , D21C1/02
CPC分类号： C13K1/02 , C12P2201/00
摘要： Disclosed is an improvement in a conventional process for C5 and C6 sugar recovery from lignocellulosic biomass for fermentation to ethanol, which process including the conventional steps of pretreatment of the biomass with steam at elevated temperature and pressure, collection of C5 sugars from hemicellulose breakdown, cellulose hydrolysis and collection of C6 sugars from cellulose breakdown. The improvement includes conditioning of the biomass prior to the pretreatment step by heating the biomass with steam for a time period between 5 minutes to 60 minutes to achieve a steam treated biomass having a temperature of about 80 to 100oC; and adjusting a moisture content of the steam treated biomass to about 45% to 80%. An increased recovery of C5 and C6 sugars is achieved compared to the conventional process
摘要翻译：公开了用于发酵到乙醇的木质纤维素生物质的C5和C6糖回收的常规方法的改进，该方法包括在高温和高压下用蒸汽预处理生物质的常规步骤，从半纤维素分解中收集C5糖，从纤维素分解中收集C5糖从纤维素分解中水解和收集C6糖。该改进包括在预处理步骤之前通过用蒸汽加热生物质在5分钟至60分钟之间的时间段来调节生物质，以实现温度为约80至100℃的经蒸汽处理的生物质; 并将蒸汽处理的生物质的水分含量调节至约45％至80％。与常规方法相比，实现了C5和C6糖的增加的恢复

5. 发明申请

WO2010121367A1 FRACTIONATION OF BIOMASS FOR CELLULOSIC ETHANOL AND CHEMICAL PRODUCTION 审中-公开
标题翻译：生物质的分解用于纤维素乙醇和化学生产
公开(公告)号：WO2010121367A1
公开(公告)日：2010-10-28
申请号：PCT/CA2010/000582
申请日：2010-04-23
申请人： GREENFIELD ETHANOL INC. , DOTTORI, Frank, A. , BENSON , Robert , Ashley , Cooper , BENECH, Regis-Olivier
发明人： DOTTORI, Frank, A. , BENSON , Robert , Ashley , Cooper , BENECH, Regis-Olivier
IPC分类号： C08H8/00 , B01D36/00
CPC分类号： C08H8/00 , C08B37/0057 , Y02E50/16
摘要： A process is defined for the continuous steam pretreatment and fractionation of corn cobs and low lignin lignocellulosic biomass to produce a concentrated cellulose solid stream that is sensitive to enzymatic hydrolysis. Valuable chemicals are recovered by fractionating the liquid and vapor stream composed of hydrolysis and degradation products of the hemicellulose. Cellulosic derived glucose is produced for fermentation to biofuels. A hemicellulose concentrate is recovered that can be converted to value added products including ethanol.
摘要翻译：定义了一种用于玉米芯和低木质素木质纤维素生物质的连续蒸汽预处理和分馏以产生对酶水解敏感的浓缩纤维素固体物流的方法。通过分离由半纤维素的水解和降解产物组成的液体和蒸汽流来回收有价值的化学品。生产用于发酵的生物燃料的纤维素衍生的葡萄糖。回收可以转化成包括乙醇在内的增值产品的半纤维素浓缩物。

6. 发明申请

WO2010121367A8 FRACTIONATION OF BIOMASS FOR CELLULOSIC ETHANOL AND CHEMICAL PRODUCTION 审中-公开
标题翻译：生物质的分解用于纤维素乙醇和化学生产
公开(公告)号：WO2010121367A8
公开(公告)日：2011-01-06
申请号：PCT/CA2010000582
申请日：2010-04-23
申请人： GREENFIELD ETHANOL INC , DOTTORI FRANK A , BENSON ROBERT ASHLEY COOPER , BENECH REGIS-OLIVIER
发明人： DOTTORI FRANK A , BENSON ROBERT ASHLEY COOPER , BENECH REGIS-OLIVIER
IPC分类号： C08H8/00 , B01D36/00
CPC分类号： C08H8/00 , C08B37/0057 , Y02E50/16
摘要： A process is defined for the continuous steam pretreatment and fractionation of corn cobs and low lignin lignocellulosic biomass to produce a concentrated cellulose solid stream that is sensitive to enzymatic hydrolysis. Valuable chemicals are recovered by fractionating the liquid and vapor stream composed of hydrolysis and degradation products of the hemicellulose. Cellulosic derived glucose is produced for fermentation to biofuels. A hemicellulose concentrate is recovered that can be converted to value added products including ethanol.
摘要翻译：定义了一种用于玉米芯和低木质素木质纤维素生物质的连续蒸汽预处理和分馏以产生对酶水解敏感的浓缩纤维素固体物流的方法。通过分离由半纤维素的水解和降解产物组成的液体和蒸汽流来回收有价值的化学品。生产用于发酵的生物燃料的纤维素衍生的葡萄糖。回收可以转化成包括乙醇在内的增值产品的半纤维素浓缩物。

7. 发明申请

WO2010111775A1 FED BATCH PROCESS FOR BIOCHEMICAL CONVERSION OF LIGNOCELLULOSIC BIOMASS TO ETHANOL 审中-公开
标题翻译： LEDOCELLULOSIC生物质生物化学转化为乙醇的FED批处理
公开(公告)号：WO2010111775A1
公开(公告)日：2010-10-07
申请号：PCT/CA2010/000456
申请日：2010-03-31
申请人： GREENFIELD ETHANOL INC. , BENSON, Robert, Ashley, Cooper , BENECH, Régis-Olivier
发明人： BENSON, Robert, Ashley, Cooper , BENECH, Régis-Olivier
IPC分类号： C12P19/14 , C12P19/00
CPC分类号： C12P7/10 , C12P19/02 , Y02E50/16
摘要： A method for optimization of a fed-batch lignocellulosic biomass hydrolysis process wherein the hydrolysis time is minimized by controlling the feed addition volume and / or batch addition frequency of prehydrolysed lignocellulosic feed and optionally the enzyme feed. The increase over time in hydrolysate consistency and volume and / or concentration of sugars released in the reactor, so that the enzymatic hydrolysis is controlled, significantly reduces the impact of cellulase feedback inhibition, especially for enzyme contents lower than 0.5%. The overall time to reach conversion of the total prehydrolysate feed is reduced significantly where the batch addition frequency is equal to one batch each time 70% to 90% of a theoretical cellulose to glucose conversion is reached, preferably when 80% conversion is reached. At an enzyme load of 0.3% in the reaction mixture, the optimum frequency each time 80% conversion was reached was found to be one batch every 105 minutes.
摘要翻译：用于优化补料分批木质纤维素生物质水解方法的方法，其中通过控制预水解木质纤维素进料和任选的酶进料的进料添加体积和/或批添加频率来最小化水解时间。在反应器中释放的糖的水解稠度和体积和/或浓度随时间的增加，使得酶水解被控制，显着降低了纤维素酶反馈抑制的影响，特别是对于低于0.5％的酶含量。每次当达到理论纤维素至葡萄糖转化率的70％至90％时，优选当达到80％转化率时，批量添加频率等于一批时，总的预水解原料进料的总转化时间显着降低。在反应混合物中的酶负荷为0.3％时，每次达到80％转化率的最佳频率每105分钟发现一次。

8. 发明申请

WO2012061939A8 CONTINUOUS PROCESS FOR THE PRODUCTION OF ETHANOL FROM LIGNOCELLULOSIC BIOMASS 审中-公开
标题翻译：连续生产乙醇的连续工艺
公开(公告)号：WO2012061939A8
公开(公告)日：2012-08-02
申请号：PCT/CA2011050692
申请日：2011-11-08
申请人： GREENFIELD ETHANOL INC , DOTTORI FRANK A , BENSON ROBERT ASHLEY COOPER , BENECH REGIS-OLIVIER
发明人： DOTTORI FRANK A , BENSON ROBERT ASHLEY COOPER , BENECH REGIS-OLIVIER
IPC分类号： C12P7/10 , C08H8/00 , C12P19/00
CPC分类号： C12P7/10 , Y02E50/16
摘要： A continuous process for the recovery of ethanol from hemicellulose and cellulose from lignocellulosic biomass. Yield of fermentable sugars can be maximized by continuous operation of the pre-treatment system and careful selection of pretreatment conditions including the addition of only small amounts of dilute mineral acid and low pressure. With this approach, the xylose component that is mainly present in its unfermentable oligomeric form in known pre-hydrolysis Kraft processes can be recovered more efficiently and as a monomer that can be fermented by xylose fermenting yeasts and bacteria. Due to the use of only dilute acids, there is a very low loss of glucose and xylose hence very low production of toxic chemicals (e.g. HMF, furfural) in the pretreatment step. The resulting overall fermentation efficiency of both hexose and pentose sugars is 90 % of the theoretical maximum.
摘要翻译：从木质纤维素生物质从半纤维素和纤维素回收乙醇的连续方法。可以通过预处理系统的连续操作和仔细选择预处理条件（包括仅添加少量稀无机酸和低压）来最大化可发酵糖的产率。通过这种方法，在已知的预水解牛皮纸方法中主要以其不可发酵的低聚形式存在的木糖组分可以更有效地回收，并且可以作为可通过木糖发酵酵母和细菌而发酵的单体。由于仅使用稀酸，葡萄糖和木糖的损失非常低，因此在预处理步骤中有毒化学品（例如HMF，糠醛）的产生非常低。所得到的己糖和戊糖的总发酵效率为理论最大值的90％。

9. 发明申请

WO2012058776A1 BAGASSE FRACTIONATION FOR CELLULOSIC ETHANOL AND CHEMICAL PRODUCTION 审中-公开
标题翻译： BAGASSE分解用于纤维素乙醇和化学生产
公开(公告)号：WO2012058776A1
公开(公告)日：2012-05-10
申请号：PCT/CA2011/050689
申请日：2011-11-04
申请人： GREENFIELD ETHANOL INC. , DOTTORI, Frank, A. , BENSON, Robert, Ashley, Cooper , BENECH, Régis-Olivier
发明人： DOTTORI, Frank, A. , BENSON, Robert, Ashley, Cooper , BENECH, Régis-Olivier
IPC分类号： C08H8/00 , C12P19/00 , C12P7/10
CPC分类号： D21C1/02 , C08H8/00 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , C13K1/02 , C13K13/002 , D21C1/06 , D21C11/0007 , Y02E50/16
摘要： A process is defined for the continuous steam pretreatment and fractionation of bagasse to produce a concentrated cellulose solid stream that is sensitive to enzymatic hydrolysis. Valuable chemicals are recovered by fractionating the liquid and vapor stream composed of hydrolysis and degradation products of the hemicellulose. Cellulosic derived glucose is produced for fermentation to biofuels. A hemicellulose concentrate is recovered that can be converted to value added products including ethanol.
摘要翻译：定义了一种用于连续蒸汽预处理和甘蔗渣分级以产生对酶水解敏感的浓缩纤维素固体物流的方法。通过分离由半纤维素的水解和降解产物组成的液体和蒸汽流来回收有价值的化学品。生产用于发酵的生物燃料的纤维素衍生的葡萄糖。回收可以转化成包括乙醇在内的增值产品的半纤维素浓缩物。

10. 发明申请

WO2010121366A1 SEPARATION OF REACTIVE CELLULOSE FROM LIGNOCELLULOSIC BIOMASS WITH HIGH LIGNIN CONTENT 审中-公开
标题翻译：具有高木质素含量的细菌生物量的反应性纤维素的分离
公开(公告)号：WO2010121366A1
公开(公告)日：2010-10-28
申请号：PCT/CA2010/000581
申请日：2010-04-23
申请人： GREENFIELD ETHANOL INC. , DOTTORI, Frank, A. , BENSON , Robert , Ashley , Cooper , BENECH, Régis-Olivier
发明人： DOTTORI, Frank, A. , BENSON , Robert , Ashley , Cooper , BENECH, Régis-Olivier
IPC分类号： C08H8/00 , C13K13/00
CPC分类号： D21C3/20 , C08H8/00 , C12P7/10 , C12P2201/00 , D21B1/36 , D21C3/04 , D21C3/222 , D21C11/0007 , Y02E50/16
摘要： A process for separating the components of lignocellulosic biomass for the purpose of producing a pure reactive cellulose is disclosed. The process has two stages. In the first stage, the lignocellulosic biomass is pretreated with steam, with or without an acid catalyst and then pressed, with or without the presence of an eluent. to remove hemicellulose and other impurities. In the second stage, the pretreated biomass is extracted with a solvent such as ethanol with or without acid catalysts in order to remove lignin and release a purified cellulose stream. The extracted cellulose is then rapidly decompressed to rupture the fibrous structure. The process provides a purified cellulose stream that is relatively easy to hydrolyze with enzymes and ferment to biofuels and other chemicals such as ethanol.
摘要翻译：公开了一种用于分离用于生产纯反应性纤维素的木质纤维素生物质组分的方法。这个过程有两个阶段。在第一阶段中，用或不用酸催化剂的蒸汽预处理木质纤维素生物质，然后在存在或不存在洗脱液的情况下压制。去除半纤维素和其他杂质。在第二阶段中，用或不用酸催化剂的溶剂如乙醇提取预处理的生物质，以除去木质素并释放纯化的纤维素流。然后将提取的纤维素快速减压以破坏纤维结构。该方法提供了一种纯化的纤维素流，其相对容易用酶水解并发酵成生物燃料和其它化学物质如乙醇。

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