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1. 发明授权

EP0395292B1 Generation of specific probes for target nucleotide sequences 失效
标题翻译：特异性探针的产生与靶核苷酸序列
公开(公告)号：EP0395292B1
公开(公告)日：1997-03-12
申请号：EP90304164.8
申请日：1990-04-18
申请人： Barry, Thomas Gerard , Gannon, Bernard Francis Xavier , EOLAS (trading as BioResearch Ireland) - The Irish Science and Technology Agency , Powell, Richard , UNIVERSITY COLLEGE GALWAY
发明人： Barry, Thomas Gerard , Gannon, BernardFrancis Xavier , Powell, Richard
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/686

2. 发明公开

EP0395292A2 Generation of specific probes for target nucleotide sequences 失效
标题翻译： Erzeugung von spezifischen Sonden gegen Ziel-Nukleotidsequenzen。
公开(公告)号：EP0395292A2
公开(公告)日：1990-10-31
申请号：EP90304164.8
申请日：1990-04-18
申请人： Barry, Thomas Gerard , Gannon, Bernard Francis Xavier , EOLAS (trading as BioResearch Ireland) - The Irish Science and Technology Agency , Powell, Richard , UNIVERSITY COLLEGE GALWAY
发明人： Barry, Thomas Gerard , Gannon, BernardFrancis Xavier , Powell, Richard
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/686
摘要： A method for generating DNA probes specific for an organism and capable of distinguishing in a non-empirical manner between species. The method comprises amplifying, using a pair of oligonucleotide primers, a variable region of the genome of a number of phylogenetically related organisms to, or of a number of organisms suspected of being present in a given sample containing, said organism to be identified and having said variable region in its genome, at least one of said primers corresponding to a DNA sequence known or suspected of being conserved in said organisms, determining the sequence of the amplified region, selecting said sequence or a portion thereof to generate said probe specific for said organism to be identified by comparison with said other amplified regions and defining the hybridization conditions required to obtain a specific signal based on the precise nucleotide sequence of the selected probe. Specific probes are disclosed for a variety of species including Aeromonas hydrophila, Aeromonas salmonicida, Clostridium difficile, Mycobacterium bovis, Mycobacterium tuberculosis and Salmonella typhimurium.
摘要翻译：一种用于产生对生物体特异的DNA探针并且能够以非经验方式区分物种的方法。所述方法包括使用一对寡核苷酸引物将许多系统发育相关生物的基因组的可变区扩增至怀疑存在于含有待鉴定的所述生物体的给定样品中的多个生物体的可能区域，并具有所述可变区在其基因组中，所述引物中的至少一个对应于已知或怀疑在所述生物体中保守的DNA序列，确定扩增区的序列，选择所述序列或其一部分以产生对于所述通过与所述其他扩增区域进行比较来鉴定生物，并基于所选探针的精确核苷酸序列定义获得特定信号所需的杂交条件。公开了针对各种物种的特异性探针，包括嗜水气单胞菌，鲑气单胞菌，艰难梭菌，牛分枝杆菌，结核分枝杆菌和鼠伤寒沙门氏菌。

3. 发明公开

EP0395292A3 Generation of specific probes for target nucleotide sequences 失效
标题翻译：目标核素序列特异性探针的产生
公开(公告)号：EP0395292A3
公开(公告)日：1992-03-04
申请号：EP90304164.8
申请日：1990-04-18
申请人： Barry, Thomas Gerard , Gannon, Bernard Francis Xavier , EOLAS (trading as BioResearch Ireland) - The Irish Science and Technology Agency , Powell, Richard , UNIVERSITY COLLEGE GALWAY
发明人： Barry, Thomas Gerard , Gannon, BernardFrancis Xavier , Powell, Richard
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/689 , C12Q1/686
摘要： A method for generating DNA probes specific for an organism and capable of distinguishing in a non-empirical manner between species. The method comprises amplifying, using a pair of oligonucleotide primers, a variable region of the genome of a number of phylogenetically related organisms to, or of a number of organisms suspected of being present in a given sample containing, said organism to be identified and having said variable region in its genome, at least one of said primers corresponding to a DNA sequence known or suspected of being conserved in said organisms, determining the sequence of the amplified region, selecting said sequence or a portion thereof to generate said probe specific for said organism to be identified by comparison with said other amplified regions and defining the hybridization conditions required to obtain a specific signal based on the precise nucleotide sequence of the selected probe. Specific probes are disclosed for a variety of species including Aeromonas hydrophila, Aeromonas salmonicida, Clostridium difficile, Mycobacterium bovis, Mycobacterium tuberculosis and Salmonella typhimurium.

4. 发明专利

IE81145B1 Generation of specific probes for target nucleotide sequences 未知
公开(公告)号：IE81145B1
公开(公告)日：2000-05-03
申请号：IE128789
申请日：1989-04-20
申请人： BARRY THOMAS GERARD , GANNON BERNARD FRANCIS XAVIER , POWELL RICHARD , UNIV GALWAY COLLEGE , FORFAS
发明人： BARRY THOMAS GERARD , GANNON BERNARD FRANCIS XAVIER , POWELL RICHARD
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/686 , C12Q1/689 , C12R1/01 , C12R1/07 , C12R1/145 , C12R1/185 , C12R1/42 , C12R1/425 , C12R1/44 , C12R1/46 , C12P19/34 , C07H21/04 , C12R1/32 , C12R1/325
摘要： A method for generating DNA probes specific for an organism and capable of distinguishing in a non-empirical manner between species. The method comprises amplifying, using a pair of oligonucleotide primers, a variable region of the genome of a number of phylogenetically related organisms to, or of a number of organisms suspected of being present in a given sample containing, said organism to be identified and having said variable region in its genome, at least one of said primers corresponding to a DNA sequence known or suspected of being conserved in said organisms, determining the sequence of the amplified region, selecting said sequence or a portion thereof to generate said probe specific for said organism to be identified by comparison with said other amplified regions and defining the hybridization conditions required to obtain a specific signal based on the precise nucleotide sequence of the selected probe. Specific probes are disclosed for a variety of species including Aeromonas hydrophila, Aeromonas salmonicida, Clostridium difficile, Mycobacterium bovis, Mycobacterium tuberculosis and Salmonella typhimurium.

5. 发明专利

DK0395292T3 未知
公开(公告)号：DK0395292T3
公开(公告)日：1997-09-15
申请号：DK90304164
申请日：1990-04-18
申请人： BARRY THOMAS GERARD , GANNON BERNARD FRANCIS XAVIER , UNIV GALWAY COLLEGE , POWELL RICHARD , FORFAS TRADING AS BIORESEARCH
发明人： BARRY THOMAS GERARD , GANNON BERNARD FRANCIS XAVIER , POWELL RICHARD
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/686 , C12Q1/689 , C12R1/01 , C12R1/07 , C12R1/145 , C12R1/185 , C12R1/42 , C12R1/425 , C12R1/44 , C12R1/46 , C07H21/04
摘要： A method for generating DNA probes specific for an organism and capable of distinguishing in a non-empirical manner between species. The method comprises amplifying, using a pair of oligonucleotide primers, a variable region of the genome of a number of phylogenetically related organisms to, or of a number of organisms suspected of being present in a given sample containing, said organism to be identified and having said variable region in its genome, at least one of said primers corresponding to a DNA sequence known or suspected of being conserved in said organisms, determining the sequence of the amplified region, selecting said sequence or a portion thereof to generate said probe specific for said organism to be identified by comparison with said other amplified regions and defining the hybridization conditions required to obtain a specific signal based on the precise nucleotide sequence of the selected probe. Specific probes are disclosed for a variety of species including Aeromonas hydrophila, Aeromonas salmonicida, Clostridium difficile, Mycobacterium bovis, Mycobacterium tuberculosis and Salmonella typhimurium.

6. 发明专利

DE69030131D1 未知
公开(公告)号：DE69030131D1
公开(公告)日：1997-04-17
申请号：DE69030131
申请日：1990-04-18
申请人： BARRY THOMAS GERARD , GANNON BERNARD FRANCIS X , EOLAS IRISH SCIENCE & TECH , POWELL RICHARD , UNIV GALWAY COLLEGE
发明人： BARRY THOMAS GERARD , GANNON BERNARDFRANCIS XAVIER , POWELL RICHARD
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/686 , C12Q1/689 , C12R1/01 , C12R1/07 , C12R1/145 , C12R1/185 , C12R1/42 , C12R1/425 , C12R1/44 , C12R1/46 , C07H21/04 , C12P19/34
摘要： A method for generating DNA probes specific for an organism and capable of distinguishing in a non-empirical manner between species. The method comprises amplifying, using a pair of oligonucleotide primers, a variable region of the genome of a number of phylogenetically related organisms to, or of a number of organisms suspected of being present in a given sample containing, said organism to be identified and having said variable region in its genome, at least one of said primers corresponding to a DNA sequence known or suspected of being conserved in said organisms, determining the sequence of the amplified region, selecting said sequence or a portion thereof to generate said probe specific for said organism to be identified by comparison with said other amplified regions and defining the hybridization conditions required to obtain a specific signal based on the precise nucleotide sequence of the selected probe. Specific probes are disclosed for a variety of species including Aeromonas hydrophila, Aeromonas salmonicida, Clostridium difficile, Mycobacterium bovis, Mycobacterium tuberculosis and Salmonella typhimurium.

7. 发明专利

AU5365290A GENERATION OF SPECIFIC DNA PROBES FOR GENERA AND SPECIES DIFFERENTIATION 未知
公开(公告)号：AU5365290A
公开(公告)日：1990-10-25
申请号：AU5365290
申请日：1990-04-18
申请人： BARRY THOMAS GERARD , BIORESEARCH IRELAND A DIVISION OF EOLAS THE IRISH SCIENCE AND TECH AGENCY , GANNON BERNARD FRANCIS XAVIER , POWELL RICHARD , UNIV GALWAY COLLEGE
发明人： NAME NOT GIVEN
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/686 , C12Q1/689 , C12R1/01 , C12R1/07 , C12R1/145 , C12R1/185 , C12R1/42 , C12R1/425 , C12R1/44 , C12R1/46 , C12N15/31 , C12Q1/04 , C12N15/10
摘要： A method for generating DNA probes specific for an organism and capable of distinguishing in a non-empirical manner between species. The method comprises amplifying, using a pair of oligonucleotide primers, a variable region of the genome of a number of phylogenetically related organisms to, or of a number of organisms suspected of being present in a given sample containing, said organism to be identified and having said variable region in its genome, at least one of said primers corresponding to a DNA sequence known or suspected of being conserved in said organisms, determining the sequence of the amplified region, selecting said sequence or a portion thereof to generate said probe specific for said organism to be identified by comparison with said other amplified regions and defining the hybridization conditions required to obtain a specific signal based on the precise nucleotide sequence of the selected probe. Specific probes are disclosed for a variety of species including Aeromonas hydrophila, Aeromonas salmonicida, Clostridium difficile, Mycobacterium bovis, Mycobacterium tuberculosis and Salmonella typhimurium.

8. 发明专利

ES2100161T3 未知
公开(公告)号：ES2100161T3
公开(公告)日：1997-06-16
申请号：ES90304164
申请日：1990-04-18
申请人： BARRY THOMAS GERARD , GANNON BERNARD FRANCIS XAVIER , FORFAS TRADING AS BIORESEARCH , POWELL RICHARD , UNIV GALWAY COLLEGE
发明人： BARRY THOMAS GERARD , GANNON BERNARDFRANCIS XAVIER , POWELL RICHARD
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/686 , C12Q1/689 , C12R1/01 , C12R1/07 , C12R1/145 , C12R1/185 , C12R1/42 , C12R1/425 , C12R1/44 , C12R1/46 , C07H21/04 , C12P19/34
摘要： A method for generating DNA probes specific for an organism and capable of distinguishing in a non-empirical manner between species. The method comprises amplifying, using a pair of oligonucleotide primers, a variable region of the genome of a number of phylogenetically related organisms to, or of a number of organisms suspected of being present in a given sample containing, said organism to be identified and having said variable region in its genome, at least one of said primers corresponding to a DNA sequence known or suspected of being conserved in said organisms, determining the sequence of the amplified region, selecting said sequence or a portion thereof to generate said probe specific for said organism to be identified by comparison with said other amplified regions and defining the hybridization conditions required to obtain a specific signal based on the precise nucleotide sequence of the selected probe. Specific probes are disclosed for a variety of species including Aeromonas hydrophila, Aeromonas salmonicida, Clostridium difficile, Mycobacterium bovis, Mycobacterium tuberculosis and Salmonella typhimurium.

9. 发明专利

DE69030131T2 未知
公开(公告)号：DE69030131T2
公开(公告)日：1997-07-17
申请号：DE69030131
申请日：1990-04-18
申请人： BARRY THOMAS GERARD , GANNON BERNARD FRANCIS X , EOLAS IRISH SCIENCE & TECH , POWELL RICHARD , UNIV GALWAY COLLEGE
发明人： BARRY THOMAS GERARD , GANNON BERNARDFRANCIS XAVIER , POWELL RICHARD
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/686 , C12Q1/689 , C12R1/01 , C12R1/07 , C12R1/145 , C12R1/185 , C12R1/42 , C12R1/425 , C12R1/44 , C12R1/46 , C07H21/04 , C12P19/34
摘要： A method for generating DNA probes specific for an organism and capable of distinguishing in a non-empirical manner between species. The method comprises amplifying, using a pair of oligonucleotide primers, a variable region of the genome of a number of phylogenetically related organisms to, or of a number of organisms suspected of being present in a given sample containing, said organism to be identified and having said variable region in its genome, at least one of said primers corresponding to a DNA sequence known or suspected of being conserved in said organisms, determining the sequence of the amplified region, selecting said sequence or a portion thereof to generate said probe specific for said organism to be identified by comparison with said other amplified regions and defining the hybridization conditions required to obtain a specific signal based on the precise nucleotide sequence of the selected probe. Specific probes are disclosed for a variety of species including Aeromonas hydrophila, Aeromonas salmonicida, Clostridium difficile, Mycobacterium bovis, Mycobacterium tuberculosis and Salmonella typhimurium.

10. 发明专利

AT150091T 未知
公开(公告)号：AT150091T
公开(公告)日：1997-03-15
申请号：AT90304164
申请日：1990-04-18
申请人： BARRY THOMAS GERARD , GANNON BERNARD FRANCIS XAVIER , EOLAS TRADING AS BIORESEARCH I , POWELL RICHARD , UNIV GALWAY COLLEGE
发明人： BARRY THOMAS GERARD , GANNON BERNARDFRANCIS XAVIER , POWELL RICHARD
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/686 , C12Q1/689 , C12R1/01 , C12R1/07 , C12R1/145 , C12R1/185 , C12R1/42 , C12R1/425 , C12R1/44 , C12R1/46 , C07H21/04 , C12P19/34
摘要： A method for generating DNA probes specific for an organism and capable of distinguishing in a non-empirical manner between species. The method comprises amplifying, using a pair of oligonucleotide primers, a variable region of the genome of a number of phylogenetically related organisms to, or of a number of organisms suspected of being present in a given sample containing, said organism to be identified and having said variable region in its genome, at least one of said primers corresponding to a DNA sequence known or suspected of being conserved in said organisms, determining the sequence of the amplified region, selecting said sequence or a portion thereof to generate said probe specific for said organism to be identified by comparison with said other amplified regions and defining the hybridization conditions required to obtain a specific signal based on the precise nucleotide sequence of the selected probe. Specific probes are disclosed for a variety of species including Aeromonas hydrophila, Aeromonas salmonicida, Clostridium difficile, Mycobacterium bovis, Mycobacterium tuberculosis and Salmonella typhimurium.

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