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    • 8. 发明申请
    • FED BATCH PROCESS FOR BIOCHEMICAL CONVERSION OF LIGNOCELLULOSIC BIOMASS TO ETHANOL
    • LEDOCELLULOSIC生物质生物化学转化为乙醇的FED批处理
    • WO2010111775A1
    • 2010-10-07
    • PCT/CA2010/000456
    • 2010-03-31
    • GREENFIELD ETHANOL INC.BENSON, Robert, Ashley, CooperBENECH, Régis-Olivier
    • BENSON, Robert, Ashley, CooperBENECH, Régis-Olivier
    • C12P19/14C12P19/00
    • C12P7/10C12P19/02Y02E50/16
    • A method for optimization of a fed-batch lignocellulosic biomass hydrolysis process wherein the hydrolysis time is minimized by controlling the feed addition volume and / or batch addition frequency of prehydrolysed lignocellulosic feed and optionally the enzyme feed. The increase over time in hydrolysate consistency and volume and / or concentration of sugars released in the reactor, so that the enzymatic hydrolysis is controlled, significantly reduces the impact of cellulase feedback inhibition, especially for enzyme contents lower than 0.5%. The overall time to reach conversion of the total prehydrolysate feed is reduced significantly where the batch addition frequency is equal to one batch each time 70% to 90% of a theoretical cellulose to glucose conversion is reached, preferably when 80% conversion is reached. At an enzyme load of 0.3% in the reaction mixture, the optimum frequency each time 80% conversion was reached was found to be one batch every 105 minutes.
    • 用于优化补料分批木质纤维素生物质水解方法的方法,其中通过控制预水解木质纤维素进料和任选的酶进料的进料添加体积和/或批添加频率来最小化水解时间。 在反应器中释放的糖的水解稠度和体积和/或浓度随时间的增加,使得酶水解被控制,显着降低了纤维素酶反馈抑制的影响,特别是对于低于0.5%的酶含量。 每次当达到理论纤维素至葡萄糖转化率的70%至90%时,优选当达到80%转化率时,批量添加频率等于一批时,总的预水解原料进料的总转化时间显着降低。 在反应混合物中的酶负荷为0.3%时,每次达到80%转化率的最佳频率每105分钟发现一次。