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1. 发明申请

WO2006062398A2 METHODS FOR THE PRODUCTION AND SECRETION OF MODIFIED PEPTIDES 审中-公开
标题翻译：方法的生产和分配修饰多肽
公开(公告)号：WO2006062398A2
公开(公告)日：2006-06-15
申请号：PCT/NL2005/000842
申请日：2005-12-07
申请人： Applied NanoSystems B.V. , MOLL, Gert Nikolaas , KUIPERS, Anneke , RINK, Rick , DRIESSEN, Arnold Jacob Mathieu , KUIPERS, Oscar Paul
发明人： MOLL, Gert Nikolaas , KUIPERS, Anneke , RINK, Rick , DRIESSEN, Arnold Jacob Mathieu , KUIPERS, Oscar Paul
IPC分类号： C12N15/62 , C12P21/02
CPC分类号： C12N15/625
摘要： The invention relates to the production of heterologous polypeptides in a recombinant host cell. More specifically, it relates to the production and secretion of peptides, such as biologically active peptides, that are modified by one or more lantibiotic-synthesizing enzymes. Provided is a nucleic acid construct encoding a polypeptide comprising 1) a non-lantibiotic export signal that is recognized by a non-lantibiotic export system; 2) a lantibiotic leader peptide that is recognized by at least a lantibiotic dehydratase such as LanB and, C-terminally of said export signal and said leader peptide, 3) a peptide of interest containing one or more serine or threonine residue(s) which can be posttranslationally dehydrated by said dehydratase. Also provided is a method for producing a polypeptide in a host cell, comprising providing a host cell with a nucleic acid construct according to the invention and allowing expression and secretion of the encoded polypeptide by said host cell, wherein said host cell comprises at least a dehydratase capable of modifying said encoded polypeptide and a non-lantibiotic export system capable of secreting said modified polypeptide.
摘要翻译：本发明涉及在重组宿主细胞中生产异源多肽。更具体地说，它涉及被一种或多种羊毛硫抗生素合成酶修饰的肽（例如生物活性肽）的产生和分泌。提供编码多肽的核酸构建体，其包含1）由非羊毛硫抗生素输出系统识别的非羊毛硫抗生素输出信号; 2）被至少一种羊毛硫抗生素脱水酶如LanB和所述输出信号和所述前导肽的C端识别的羊毛硫抗生素前导肽，3）一种含有一个或多个丝氨酸或苏氨酸残基的感兴趣的肽，可以通过所述脱水酶进行翻译后脱水。还提供了用于在宿主细胞中产生多肽的方法，包括向宿主细胞提供根据本发明的核酸构建体并且允许所述宿主细胞表达和分泌编码的多肽，其中所述宿主细胞包含至少一个能够修饰所述编码的多肽的脱水酶和能够分泌所述修饰的多肽的非羊毛硫抗生素输出系统。

2. 发明申请

WO2006062398A3 METHODS FOR THE PRODUCTION AND SECRETION OF MODIFIED PEPTIDES 审中-公开
标题翻译：改性肽的生产和分泌方法
公开(公告)号：WO2006062398A3
公开(公告)日：2006-10-19
申请号：PCT/NL2005000842
申请日：2005-12-07
申请人： APPLIED NANOSYSTEMS BV , MOLL GERT NIKOLAAS , KUIPERS ANNEKE , RINK RICK , DRIESSEN ARNOLD JACOB MATHIEU , KUIPERS OSCAR PAUL
发明人： MOLL GERT NIKOLAAS , KUIPERS ANNEKE , RINK RICK , DRIESSEN ARNOLD JACOB MATHIEU , KUIPERS OSCAR PAUL
IPC分类号： C12N15/62 , C12P21/02
CPC分类号： C12N15/625
摘要： The invention relates to the production of heterologous polypeptides in a recombinant host cell. More specifically, it relates to the production and secretion of peptides, such as biologically active peptides, that are modified by one or more lantibiotic-synthesizing enzymes. Provided is a nucleic acid construct encoding a polypeptide comprising 1) a non-lantibiotic export signal that is recognized by a non-lantibiotic export system; 2) a lantibiotic leader peptide that is recognized by at least a lantibiotic dehydratase such as LanB and, C-terminally of said export signal and said leader peptide, 3) a peptide of interest containing one or more serine or threonine residue(s) which can be posttranslationally dehydrated by said dehydratase. Also provided is a method for producing a polypeptide in a host cell, comprising providing a host cell with a nucleic acid construct according to the invention and allowing expression and secretion of the encoded polypeptide by said host cell, wherein said host cell comprises at least a dehydratase capable of modifying said encoded polypeptide and a non-lantibiotic export system capable of secreting said modified polypeptide.
摘要翻译：本发明涉及在重组宿主细胞中产生异源多肽。更具体地，本发明涉及由一种或多种羊毛硫素合成酶修饰的肽（例如生物活性肽）的产生和分泌。本发明提供了编码多肽的核酸构建体，其包括1）非耐药物生物出口信号，其被非拉丁生物出口系统识别; 2）由至少一种羊毛硫抗生素脱水酶（例如LanB）和所述出口信号和所述前导肽的C-末端识别的羊毛硫素前导肽，3）感兴趣的肽含有一个或多个丝氨酸或苏氨酸残基，其中可以通过所述脱水酶进行翻译后脱水。还提供了在宿主细胞中产生多肽的方法，其包括向宿主细胞提供根据本发明的核酸构建体，并允许所述宿主细胞表达和分泌编码的多肽，其中所述宿主细胞至少包含能够修饰所述编码的多肽的脱水酶和能够分泌所述修饰的多肽的非拉链菌素出口系统。

3. 发明申请

WO2007096419A1 IMPROVED CEPHALOSPORIN PRODUCTION 审中-公开
标题翻译：改善CEPHALOSPORIN生产
公开(公告)号：WO2007096419A1
公开(公告)日：2007-08-30
申请号：PCT/EP2007/051755
申请日：2007-02-23
申请人： DSM IP ASSETS B.V. , HANS, Marcus , BOVENBERG, Roelof, Ary, Lans , VAN DEN BERG, Marco Alexander , DRIESSEN, Arnold Jacob Mattieu , NIJLAND, Jeroen Gerben
发明人： HANS, Marcus , BOVENBERG, Roelof, Ary, Lans , VAN DEN BERG, Marco Alexander , DRIESSEN, Arnold Jacob Mattieu , NIJLAND, Jeroen Gerben
IPC分类号： C07D501/00 , C12P35/06 , C12N15/81 , C12R1/645
CPC分类号： C07K14/37 , C12P35/06
摘要： The present invention relates to a cephalosporin-producing microorganism characterized in that it has been transformed with a heterologous CefT gene and whereby the ratio of the cephalosporin production capacity of the microorganism transformed with the heterologous CefT gene compared to the cephalosporin production capacity of the microorganism not being transformed with the heterologous CefT gene is at least 1.2. Furthermore, the present invention provides a method for the production of the cephalosporin-producing microorganism as well as a process for the fermentative production of cephalosporins using the cephalosporin-producing microorganism.
摘要翻译：本发明涉及产生头孢菌素的微生物，其特征在于其已经用异源CefT基因转化，由此与异源CefT基因转化的微生物的头孢菌素生产能力与微生物的头孢菌素生产能力的比率不用异源CefT基因转化至少1.2。此外，本发明提供了生产头孢菌素生产微生物的方法以及使用头孢菌素生产微生物发酵生产头孢菌素的方法。

4. 发明申请

WO2003099862A1 EXPORT AND MODIFICATION OF (POLY)PEPTIDES IN THE LANTIBIOTIC WAY 审中-公开
标题翻译：（POLY）PEPTIDES在生态方式的出口和修改
公开(公告)号：WO2003099862A1
公开(公告)日：2003-12-04
申请号：PCT/NL2003/000389
申请日：2003-05-26
申请人： APPLIED NANOSYSTEMS B.V. , MOLL, Gert, Nikolaas , LEENHOUTS, Cornelis, Johannes , KUIPERS, Oscar, Paul , DRIESSEN, Arnold, Jacob, Mathieu
发明人： MOLL, Gert, Nikolaas , LEENHOUTS, Cornelis, Johannes , KUIPERS, Oscar, Paul , DRIESSEN, Arnold, Jacob, Mathieu
IPC分类号： C07K14/315
CPC分类号： C12P21/02 , C07K14/315 , C07K2319/02
摘要： The invention includes a method for harvesting a polypeptide produced by a host cell, wherein the polypeptide has not undergone intra-cellular post-translational modification, such as dehydration of a serine or a threonine, and/or thioether bridge formation. The invention also includes a method for producing thioether containing peptides and dehydroalanine/dehydrobutyrine-containing peptides, wherein extracellularly thioether rings may be formed.
摘要翻译：本发明包括用于收获由宿主细胞产生的多肽的方法，其中所述多肽未经历细胞内翻译后修饰，例如丝氨酸或苏氨酸的脱水和/或硫醚桥接形成。本发明还包括制备含硫醚的肽和含有脱氢丙氨酸/脱氢丁啶的肽的方法，其中可以形成细胞外硫醚环。

5. 发明申请

WO0132904A3 METHOD FOR ENHANCING SECRETION OF BETA-LACTAM COMPOUNDS 审中-公开
标题翻译：用于增强BETA-LACTAM化合物分泌的方法
公开(公告)号：WO0132904A3
公开(公告)日：2001-11-08
申请号：PCT/EP0011489
申请日：2000-11-03
申请人： DSM NV , BERG VAN DEN MARCO ALEXANDER , BOVENBERG ROELOF ARY LANS , DRIESSEN ARNOLD JACOB MATTIEU , KONINGS WILHELMUS NICOLAAS , SCHUURS THEO AUKE , NIEBOER MAARTEN , WESTERLAKEN ILJA
发明人： BERG VAN DEN MARCO ALEXANDER , BOVENBERG ROELOF ARY LANS , DRIESSEN ARNOLD JACOB MATTIEU , KONINGS WILHELMUS NICOLAAS , SCHUURS THEO AUKE , NIEBOER MAARTEN , WESTERLAKEN ILJA
IPC分类号： C07K14/385 , C12N9/12 , C12N9/14 , C12N15/31 , C12P17/18 , C12P35/00 , C12P37/00
CPC分类号： C07K14/385 , C12P37/00
摘要： An method for enhancing the secretion of a beta -lactam compound from a micro-organism is described, together with an isolated polynucleotide encoding the ABC transporter protein responsible for said enhanced secretion. Furthermore an isolated poylnucleotide encoding an ABC transporter is claimed. Method for producing beta -lactam compounds in a micro-organism comprising the step of enhancing ABC-transporter activity of said micro-organism and subsequently culturing said micro-organism under conditions allowing the production of said beta -lactam compounds is also disclosed.
摘要翻译：与来自微生物的β-内酰胺化合物分泌增强的方法一起描述了编码负责所述增强分泌的ABC转运蛋白的分离的多核苷酸。此外，要求保护编码ABC转运蛋白的孤立的寡核苷酸。还公开了在微生物中产生β-内酰胺化合物的方法，其包括增强所述微生物的ABC-转运蛋白活性并随后在允许产生所述β-内酰胺化合物的条件下培养所述微生物的步骤。

6. 发明申请

WO03099862A8 EXPORT AND MODIFICATION OF (POLY)PEPTIDES IN THE LANTIBIOTIC WAY 审中-公开
标题翻译：（POLY）PEPTIDES在生态方式的出口和修改
公开(公告)号：WO03099862A8
公开(公告)日：2004-03-11
申请号：PCT/NL0300389
申请日：2003-05-26
申请人： APPLIED NANOSYSTEMS BV , MOLL GERT NIKOLAAS , LEENHOUTS CORNELIS JOHANNES , KUIPERS OSCAR PAUL , DRIESSEN ARNOLD JACOB MATHIEU
发明人： MOLL GERT NIKOLAAS , LEENHOUTS CORNELIS JOHANNES , KUIPERS OSCAR PAUL , DRIESSEN ARNOLD JACOB MATHIEU
IPC分类号： A61K38/14 , C07K9/00 , C07K14/315 , C12N5/06 , C12P21/02
CPC分类号： C12P21/02 , C07K14/315 , C07K2319/02
摘要： The invention includes a method for harvesting a polypeptide produced by a host cell, wherein the polypeptide has not undergone intra-cellular post-translational modification, such as dehydration of a serine or a threonine, and/or thioether bridge formation. The invention also includes a method for producing thioether containing peptides and dehydroalanine/dehydrobutyrine-containing peptides, wherein extracellularly thioether rings may be formed.
摘要翻译：本发明包括用于收获由宿主细胞产生的多肽的方法，其中所述多肽未经历细胞内翻译后修饰，例如丝氨酸或苏氨酸的脱水和/或硫醚桥接形成。本发明还包括一种生产含硫醚的肽和含有脱氢丙氨酸/脱氢丁啶的肽的方法，其中可以形成细胞外硫醚环。

7. 发明专利

GB247034A Improvements in resilient insoles, socks, and instep supports for insertion in bootsand shoes 未知
公开(公告)号：GB247034A
公开(公告)日：1926-02-11
申请号：GB970325
申请日：1925-04-14
申请人： ARNOLD JACOB MARKS
IPC分类号： A43B7/22
摘要： 247,034. Marks, A. J. April 14, 1925. Foot-arch supports.-A resilient insole a for boots and shoes having downwardly-directed columns c, Fig. 2, beneath the heel and/or a.rch,. has the rubber columns tapered and almost completely inserted through apertures in a plate b of compressed fibre, leather, or like inextensible material secured to the insole at f, each aperture in the member b being of slightly less area than the greatest sectional area of the rubber column c so that the column is supported near the top. A more or less rigid plate d of zinc or fibre covers the tops of the columns c. In the case of an arch support, Fig. 8, an arched metal instep support g bears on rubber columns c secured in a leather or like band h attached to the insole with or without similarly secured heelsupporting columns c on the insole.

8. 发明专利

DK89799C 未知
公开(公告)号：DK89799C
公开(公告)日：1960-10-03
申请号：DK543354
申请日：1954-06-15
申请人： BUSK ARNOLD JACOB , CHRISTENSEN ADOLF VILHELM , CHRISTENSEN AKSEL HALD , CHRISTENSEN POUL HALD
发明人： BUSK ARNOLD JACOB , CHRISTENSEN ADOLF VILHELM , CHRISTENSEN AKSEL HALD , CHRISTENSEN POUL HALD
IPC分类号： G05D20060101 , G05D

9. 发明专利

GB1037311A Rotary processing equipment 未知
公开(公告)号：GB1037311A
公开(公告)日：1966-07-27
申请号：GB372663
申请日：1963-01-29
申请人： KURT HENRY CONLEY , ARNOLD JACOB TENNER , CURTIS CLAYTON WALLACE
IPC分类号： B02C13/14 , B02C13/24
摘要： 1,037,311. Impact pulverizers. K. H. CONLEY, A. J. TENNER and C. C. WALLACE. Jan.29,1963 [Jan. 29,1962], No. 3726/63. Heading B2A. An impact pulverizer comprises a rotor 60 contained in a housing consisting of a member 33 and a hopper 70 releasably secured thereto, material fed to the rotor by a shoot 44 releasably secured to the member 33 being prevented from impinging on the member 33 by means of a liner 50 and a plate 34. The edges of the plate 34 are supported by the liner 50 which has secured thereto sockets 55 engageable by bolts 54 for detachably securing the liner to the member 33. The plate 34 has a collar portion which passes through an aperture in the member 33, the collar portion having peripheral grooves 34e engageable by bolts 47 the upper edge of the collar portion having two cutouts so that by rotating the plate the latter can be released from the bolts. Air under pressure is supplied through a fitting 41 via recessed region 34d to the interior of the housing. The hub of the rotor has mounted thereon a cutter distributor member 58 which comprises a pair of discs provided with projecting blades to break up agglomerative-particles of the input material and a further disc, above the pair of discs, provided with radial grooves to set up centrifugal movement of air. Rows of impacting elements 51 secured to the liner are interspersed with rows of impacting elements 60alpha secured to the rotor. The hopper 70 is detachably secured to the member 33 and the liner 50 by means of bolts 73 and has secured thereto a wall 75, to reduce noise, and a spiral 80 between and/or through the turns of which a cooling fluid is passed. Rotatable lugs 82 screwed into sockets attached to a flange 33a of member 33 are provided with pins 82alpha and 82b to facilitate assembly of the liner and.hopper respectively.-

10. 发明专利

DK76865C 未知
公开(公告)号：DK76865C
公开(公告)日：1953-12-21
申请号：DK121250
申请日：1950-04-05
申请人： BUSK ARNOLD JACOB , CHRISTENSEN ADOLF VILHELM , CHRISTENSEN AKSEL HALD , CHRISTENSEN POUL HALD
发明人： BUSK ARNOLD JACOB , CHRISTENSEN ADOLF VILHELM , CHRISTENSEN AKSEL HALD , CHRISTENSEN POUL HALD

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