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    • 10. 发明公开
    • Kit and method for diagnosing non-pregnancy
    • 用于诊断非妊娠的试剂盒和方法
    • EP1248111A2
    • 2002-10-09
    • EP02290721.6
    • 2002-03-21
    • KOREA GREEN CROSS CORPORATIONRepublic of Korea
    • Seong, Hwan-hooCho, MinWon, Yoo-deokKim, In-wook
    • G01N33/543G01N33/74
    • G01N33/54306G01N33/54386G01N33/743
    • The present invention relates to a kit for diagnosing non-pregnancy and a method for diagnosing non-pregnancy of an animal using the same, and more particularly, to a kit for diagnosing non-pregnancy of animal comprising: an assay strip, in the form of porous membrane paper comprising a plurality of holes for moving a test sample, wherein progesterone-BSA conjugate is immobilized on one end portion as a test line and anti-IgG is immobilized on the other end portion as a control line; and anti-progesterone IgG-gold conjugate for reacting with the test sample such as blood or milk. The inventive kit for diagnosing non-pregnancy can test the non-pregnancy of milch cow without any special device about 20 days after the artificial insemination. Furthermore, more accurate result can be obtained by using the kit since the non-pregnancy diagnosis is performed by comparing color of the test line with that of the control line.
    • 用于诊断未怀孕的试剂盒和使用该试剂盒诊断未怀孕的方法技术领域本发明涉及用于诊断未怀孕的试剂盒和使用该试剂盒诊断动物未怀孕的方法,并且更具体地涉及用于诊断动物未怀孕的试剂盒,其包括: 包括用于移动测试样品的多个孔的多孔膜纸,其中孕酮-BSA缀合物固定在作为测试线的一个末端部分上,抗-IgG固定在作为对照线的另一个末端部分上; 和用于与测试样品如血液或牛奶反应的抗黄体酮IgG-金结合物。 本发明的非妊娠诊断试剂盒可以在人工授精后约20天无需任何特殊装置即可测试奶牛的未怀孕。 此外,通过使用试剂盒可以获得更准确的结果,因为通过比较测试线和对照线的颜色进行非妊娠诊断。