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    • 5. 发明申请
    • CELL CULTURE APPARATUS, METHODS FOR CELL CULTIVATION BY USING THE SAME, AND CELL CULTURE INCUBATOR COMPRISING THE SAME
    • WO2023036942A1
    • 2023-03-16
    • PCT/EP2022/075131
    • 2022-09-09
    • FINNADVANCE OY
    • SINGH, Prateek
    • C12M1/32C12M1/00C12M1/12
    • The present disclosure relates generally to the field of biotechnology and, in particular, to a cell culture apparatus, methods for cell cultivation by using the cell culture apparatus, and a cell culture incubator comprising the cell culture apparatus. The cell culture apparatus comprises a plurality of cell culture modules arranged adjacent to each other. Each cell culture module comprises two or more culture medium reservoirs connected by two or more flow channels. The flow channels go through a basal chamber arranged under an apical chamber. The apical and basal chambers are separated by a porous membrane. The basal chamber has a bottom part arranged higher than a bottom part of each of the culture medium reservoirs. In this apparatus configuration, flows of culture media may be perfused and regulated between the culture medium reservoirs in each cell culture module by either placing the apparatus on a rocking platform or connecting the apparatus to a pneumatic pump. Moreover, the apparatus thus configured may cultivate cells of the same or different types on the basal surface of the porous membranes in the cell culture modules under unform and controlled in vitro conditions. The deposition of the cells on the basal surface of the porous membranes may be provided without having to invert the apparatus and to use high concentrations of the cells in the culture medium.
    • 7. 发明申请
    • PILE ASSEMBLY
    • WO2023030631A1
    • 2023-03-09
    • PCT/EP2021/074249
    • 2021-09-02
    • R KONSULTOINTI OY
    • RÖNNBLAD, Göran
    • E02D5/52
    • A pile assembly (1) comprising a concrete pile (2), a pile member (3) and a connecting arrangement for connecting an end (5) of the pile (2) and the pile member (3) together. The connecting arrangement comprises a pile joint (6) at the end of the concrete pile. The pile joint (6) comprises at least one locking member (7) placed in the concrete at the end of the pile, the locking member comprising an inlet part (8) comprising an inlet hole (11) having a first end (12) at the end (5) of the pile, a locking chamber (16) extending from a second end (13) of the inlet hole (11) and having an inner diameter (Wlc) larger than that (Wih) of the inlet hole (11). The connecting arrangement comprises at least one connecting member (19) having a first end (20) inserted through the inlet hole (11) into the locking chamber (16) and a second end (21) connected to the pile member (3). A bending member (23) is arranged in the locking chamber (16). The bending member (23) is configured to bend and/or expand the connecting member (19) against a surface of the inlet hole (11) and/or against an inner surface of the locking chamber (16) when the connecting member (19) and the bending member (23) are pressed against each other.