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    • 10. 发明公开
    • BI-DIRECTIONAL CONTINUOUS PERFUSION BIOREACTOR FOR TRIDIMENSIONAL CULTURE OP MAMMAL TISSUE SUBSTITUTES
    • BIDIREKTIONALER KONTINUIERLICHER PERFUSIONSBIOREAKTORFÜRDREIDIMENSIONALE KULTUR AUSSÄGGERGEWEBESUBSTITUTEN
    • EP2373780A1
    • 2011-10-12
    • EP09793624.9
    • 2009-11-27
    • Association For The Advancement Of Tissue Engineering And Cell Based Technologies & Therapies (A4TEC)
    • GARDEL, LeandroGOMES,Maria Manuela EstimaREIS, Rui Luís Gonçalves dos
    • C12M3/00
    • C12M25/14C12M27/10C12M29/10C12M35/04
    • The Bio-reactor (BCPB) presents an advanced innovative system of culture for growth, of three-dimensional mammal tissue in hybrid materials (cell-material; scaffolds) in the tissue engineering and regenerative medicine fields. The BCFB induces mechanical cellular stimuli by creating shear forces caused by the flow perfusion using different pressure gradients, controlled by two pumps, a vacuum and a peristaltic one, positioned within a circular flow system designed for the BCFB and also through rotational engines positioned in specific locations. Another innovation is the fact that with this bioreastor we are able to grow hybrid materials (scaffolds with cells) of large dimensions, through the control of the perfusion and flow gradient within the scaffolds, thereby obtaining access of the nutrients to the interior of the material and the removal of the metabolic products of the interior of the material, a technical problem found in the bioreactors commercially available nowadays.
    • 生物反应器(BCPB)为组织工程和再生医学领域的混合材料(细胞材料;支架)中的三维哺乳动物组织的生长提供了先进的创新体系。 BCFB通过产生由流动灌注引起的剪切力,通过使用不同的压力梯度产生剪切力,该压力梯度由两个泵控制,一个真空和一个蠕动的泵,定位在为BCFB设计的圆形流动系统中,以及通过位于特定的 位置。 另一个创新是,通过使用这种生物反应器,我们能够通过控制支架内的灌注和流动梯度来生长大尺寸的杂交材料(具有细胞的支架),从而获得营养物质到材料的内部 以及去除材料内部的代谢产物,这是现今市售的生物反应器中发现的技术问题。