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    • 7. 发明公开
    • Homogeneous biospecific assay method using lanthanide chelates as labels
    • 同源biospezifische Bestimmungsmethode mit Lanthanidchelaten-Marker。
    • EP0324323A1
    • 1989-07-19
    • EP88850314.1
    • 1988-09-20
    • WALLAC OY
    • Hemmilä, IlkkaLövgren, Timo
    • G01N33/542G01N33/533C12Q1/68
    • G01N33/542G01N33/582G01N2458/40Y10S436/826
    • Method for a homogeneous biospecific affinity assay for determining the content of a substance (analyte) in a biological sample. The assay is carried out in an aqueous reaction medium by means of time-resolved fluorescence spectroscopy and with a biospecific affinity reactant labeled with a lanthanide chelate in which a lanthanide ion exhibiting ionic fluorescence is chelated by a ligand bound covalently to the reactant. The characteristic feature is

      (i) that the lanthanide chelate formed by the lanthanide ion together with the covalently bound ligand forms a fluorescent chelate, and
      (ii) that a modulator is added which stabilizes the lantha­nide chelate so that the lanthanide fluorescence as measured from the medium becomes a practically pure function of the analyte concentration therein.
    • 用于确定生物样品中物质(分析物)含量的均相生物特异性亲和测定法。 通过时间分辨荧光光谱法和用镧系元素螯合物标记的生物特异性亲和反应物在水性反应介质中进行测定,其中显示离子荧光的镧系元素离子被共价结合到反应物上的配体螯合。 其特征是(i)由镧系元素离子与共价结合的配体一起形成的镧系元素螯合物形成荧光螯合物,和(ii)添加调节剂,其使镧系元素螯合物稳定,使得镧系元素荧光从 培养基成为其中分析物浓度的实际纯度函数。 Ø