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1. 发明专利

JP2002325597A METHOD FOR PRODUCING ANTIBODY 审中-公开
公开(公告)号：JP2002325597A
公开(公告)日：2002-11-12
申请号：JP2001300890
申请日：2001-09-28
申请人： PROTEIN EXPRESS KK
发明人： SEYA TSUKASA , MATSUMOTO MISAKO
IPC分类号： C12N15/09 , A61K39/395 , C12P21/08
摘要： PROBLEM TO BE SOLVED: To provide an entirely new type method for producing an antibody, by which the antibody can efficiently be produced without needing an antigen protein-purifying process which is essential for a conventional method. SOLUTION: This method for producing an antibody against an antigen protein is characterized by culturing an animal cell integrated with a DNA encoding the antigen protein to express the antigen protein and then immunizing the same kind of an animal as that of the animal cell with the animal cell. Thereby, the invention can provide the method for producing the antibody, by which the antibody can be produced in a high antibody yield in an experimental animal consumption-reduced state without needing the purification of the antigen protein. For example, an anti-Cremp antibody originated from avian thymus gland and anti-Rae-1α antibody can be produced.

2. 发明申请

WO2009145606A1 METHOD FOR THE PRODUCTION OF PROTEINS OR PROTEIN FRAGMENTS 审中-公开
标题翻译：生产蛋白质或蛋白质片段的方法
公开(公告)号：WO2009145606A1
公开(公告)日：2009-12-03
申请号：PCT/NL2008/000137
申请日：2008-05-28
申请人： U-PROTEIN EXPRESS B.V. , ROMIJN, Roland, Antonius, Paulus , HEMRIKA, Wieger
发明人： ROMIJN, Roland, Antonius, Paulus , HEMRIKA, Wieger
IPC分类号： C12N5/06 , C12N15/67 , C12N15/63 , C12N15/85
CPC分类号： C12N15/85 , C12N15/1086
摘要： The present invention relates to a method for selecting a suitable expression construct from a plurality of expression constructs for optimizing the production of a protein or a fragment thereof in a host cell, a method for the production of proteins or fragment thereof using the selcted expression vector, to novel human embryonic kidney cells that are deficient in N -acetylglucosaminyltransferase I and stably transfected with EBNA (HEK 293E GnTI'cells) that are well suitable for use in the said method, in particular for the production of proteins or protein fragments that are suitable for X-ray studies. The invention also relates to a method to produce HEK 293E GnTI' cells and a method to confer to HEK293E GnTI' cells, the capacity to grow in suspension and to a method to confer to HEK293E GnTl' cells, the capacity to grow in serum free medium. The invention also relates to a kit comprising different vectors suitable for use of the above method for the production of proteins or protein fragments.
摘要翻译：本发明涉及从多种表达构建体中选择合适的表达构建体的方法，所述表达构建体用于优化宿主细胞中蛋白质或其片段的产生，使用筛选的表达载体产生蛋白质或其片段的方法，对于N-乙酰葡糖胺基转移酶I缺陷并且稳定转染有EBNA（HEK 293E GnTI细胞）的新型人胚胎肾细胞，其非常适合用于所述方法，特别是用于生产蛋白质或蛋白质片段适合X光检查。本发明还涉及产生HEK 293E GnTI细胞的方法和赋予HEK293E GnTI'细胞的方法，悬浮液生长能力和赋予HEK293E GnT1细胞的方法，其中无血清生长的能力中。本发明还涉及包含适用于上述生产蛋白质或蛋白质片段的方法的不同载体的试剂盒。

3. 发明申请

WO2009145606A8 METHOD FOR THE PRODUCTION OF PROTEINS OR PROTEIN FRAGMENTS 审中-公开
标题翻译：生产蛋白质或蛋白质片段的方法
公开(公告)号：WO2009145606A8
公开(公告)日：2010-02-11
申请号：PCT/NL2008000137
申请日：2008-05-28
申请人： PROTEIN EXPRESS B V U , ROMIJN ROLAND ANTONIUS PAULUS , WIEGER HEMRIKA
发明人： ROMIJN ROLAND ANTONIUS PAULUS , WIEGER HEMRIKA
IPC分类号： C12N15/63 , C12N15/67 , C12N15/85
CPC分类号： C12N15/85 , C12N15/1086
摘要： The present invention relates to a method for selecting a suitable expression construct from a plurality of expression constructs for optimizing the production of a protein or a fragment thereof in a host cell, a method for the production of proteins or fragment thereof using the selcted expression vector, to novel human embryonic kidney cells that are deficient in N-acetylglucosaminyltransferase I and stably transfected with EBNA (HEK 293E GnTI'cells) that are well suitable for use in the said method, in particular for the production of proteins or protein fragments that are suitable for X-ray studies. The invention also relates to a method to produce HEK 293E GnTI' cells and a method to confer to HEK293E GnTI' cells, the capacity to grow in suspension and to a method to confer to HEK293E GnTl' cells, the capacity to grow in serum free medium. The invention also relates to a kit comprising different vectors suitable for use of the above method for the production of proteins or protein fragments.
摘要翻译：本发明涉及从多种表达构建体中选择合适的表达构建体的方法，所述表达构建体用于优化宿主细胞中蛋白质或其片段的产生，使用筛选的表达载体产生蛋白质或其片段的方法，对于N-乙酰葡糖胺基转移酶I缺陷并且稳定转染有EBNA（HEK 293E GnTI细胞）的新型人胚胎肾细胞，其非常适合用于所述方法，特别是用于生产蛋白质或蛋白质片段适合X光检查。本发明还涉及产生HEK 293E GnTI细胞的方法和赋予HEK293E GnTI'细胞的方法，悬浮液生长能力和赋予HEK293E GnT1细胞的方法，其中无血清生长的能力中。本发明还涉及包含适用于上述生产蛋白质或蛋白质片段的方法的不同载体的试剂盒。

4. 发明公开

EP2297301A1 METHOD FOR THE PRODUCTION OF PROTEINS OR PROTEIN FRAGMENTS 审中-公开
标题翻译：用于生产蛋白质或蛋白质片段
公开(公告)号：EP2297301A1
公开(公告)日：2011-03-23
申请号：EP08766710.1
申请日：2008-05-28
申请人： U-Protein Express B.V.
发明人： ROMIJN, Roland, Antonius, Paulus , WIEGER, Hemrika
IPC分类号： C12N5/06 , C12N15/67 , C12N15/63 , C12N15/85
CPC分类号： C12N15/85 , C12N15/1086
摘要： The present invention relates to a method for selecting a suitable expression construct from a plurality of expression constructs for optimizing the production of a protein or a fragment thereof in a host cell, a method for the production of proteins or fragment thereof using the selected expression vector, to novel human embryonic kidney cells that are deficient in N-acetyl-glucosaminyltransferase I and stably transfected with EBNA (HEK 293E GnTI′cells) that are well suitable for use in the said method, in particular for the production of proteins or protein fragments that are suitable for X-ray studies. The invention also relates to a method to produce HEK 293E GnTI′ cells and a method to confer to HEK293E GnTI′ cells, the capacity to grow in suspension and to a method to confer to HEK293E GnTI′ cells, the capacity to grow in serum free medium. The invention also relates to a kit comprising different vectors suitable for use of the above method for the production of proteins or protein fragments.

5. 发明授权

EP1439210B1 aminoacyl-tRNA bound to labelled p-aminophenylalanine or tyrosine 有权转让
标题翻译：氨酰-tRNA geunden到标有对氨基苯丙氨酸或酪氨酸
公开(公告)号：EP1439210B1
公开(公告)日：2007-09-05
申请号：EP03741401.8
申请日：2003-07-15
申请人： Protein Express Co., Ltd. , Hohsaka, Takahiro
发明人： HOHSAKA, Takahiro , SISIDO, M, Dept of Bioscience and Biotechnology,
IPC分类号： C09B57/00 , C07F5/02 , C07K1/13 , C07K16/40 , C12N15/09 , G01N33/58
CPC分类号： C07B59/001 , C07F5/022 , C07F5/027 , C09B57/00 , C09B57/10 , C12P21/02
摘要： A labeled amino acid which can be transferred into a protein with the use of a protein synthesis system and a functional protein having a function originating in a labeling compound. A labeled amino acid wherein an aromatic ring is bonded to a side chain of the amino acid and a labeling compound is further bonded to the amino acid via the aromatic ring; and a functional protein having the labeled amino acid transferred thereinto. Also, a novel method for efficiently obtaining a labeled amino acid/tRNA complex is provided.

6. 发明公开

EP1439210A1 NON-NATURAL LABELED AMINO ACID AND METHOD OF CONSTRUCTING AMINO ACID/tRNA COMPLEX 有权转让
标题翻译：氨基酰基-tRNA大自然对称氨基苯丙氨酸酪氨酸
公开(公告)号：EP1439210A1
公开(公告)日：2004-07-21
申请号：EP03741401.8
申请日：2003-07-15
申请人： Protein Express Co., Ltd. , Hohsaka, Takahiro
发明人： HOHSAKA, Takahiro , SISIDO, M, Dept of Bioscience and Biotechnology,
IPC分类号： C09B57/00 , C07F5/02 , C07K1/13 , C07K16/40 , C12N15/09
CPC分类号： C07B59/001 , C07F5/022 , C07F5/027 , C09B57/00 , C09B57/10 , C12P21/02
摘要： This invention relates to: a labeled amino acid that can be introduced into a protein with the aid of a protein synthesis system; a functional protein having functions derived from a label compound; a labeled amino acid comprising an aromatic ring bound to an amino acid side chain and a label compound bound thereto via the aromatic ring; a functional protein to which the labeled amino acid has been introduced; and a novel method for effectively obtaining a labeled amino acid-tRNA complex.
摘要翻译：本发明涉及：借助于蛋白质合成系统可以引入蛋白质的标记氨基酸; 具有衍生自标记化合物的功能的功能性蛋白质; 包含与氨基酸侧链结合的芳环和经由芳环结合的标记化合物的标记氨基酸; 已经引入了标记的氨基酸的功能性蛋白质; 以及有效获得标记的氨基酸-TRNA复合物的新方法。

7. 发明专利

AU2008356942A1 Method for the production of proteins or protein fragments 未知
公开(公告)号：AU2008356942A1
公开(公告)日：2009-12-03
申请号：AU2008356942
申请日：2008-05-28
申请人： U PROTEIN EXPRESS B V
发明人： HEMRIKA WIEGER , ROMIJN ROLAND ANTONIUS PAULUS
IPC分类号： C12N5/07 , C12N15/63 , C12N15/67 , C12N15/85
摘要： The present invention relates to a method for selecting a suitable expression construct from a plurality of expression constructs for optimizing the production of a protein or a fragment thereof in a host cell, a method for the production of proteins or fragment thereof using the selected expression vector, to novel human embryonic kidney cells that are deficient in N-acetyl-glucosaminyltransferase I and stably transfected with EBNA (HEK 293E GnTI′cells) that are well suitable for use in the said method, in particular for the production of proteins or protein fragments that are suitable for X-ray studies. The invention also relates to a method to produce HEK 293E GnTI′ cells and a method to confer to HEK293E GnTI′ cells, the capacity to grow in suspension and to a method to confer to HEK293E GnTI′ cells, the capacity to grow in serum free medium. The invention also relates to a kit comprising different vectors suitable for use of the above method for the production of proteins or protein fragments.

8. 发明专利

AU2001246864B2 Hypotensors 未知
公开(公告)号：AU2001246864B2
公开(公告)日：2006-07-27
申请号：AU2001246864
申请日：2001-04-09
申请人： PROTEIN EXPRESS CO LTD
发明人： ISHIDA YUICHI , MURAKAMI KAZUO , SUZUKI FUMIAKI
IPC分类号： G01N33/50 , A61K38/00 , A61P9/12 , C07K14/47 , C12N9/64 , C12Q1/37
摘要： There are provided a method for selection of a substance which is capable of controlling activation of prorenin where an adjusting ability of the activation of prorenin by protein-protein interaction in a profragment region of prorenin as indicator is used; a prorenin activation controlling substance having a function of controlling the activation of prorenin based on protein-protein interaction by a profragment region of prorenin; and hypotensor, organ hypertrophy suppressor and arterial thickening suppressor containing the said prorenin activation controlling substance as an effective ingredient.

9. 发明专利

AU2003281613A1 NON-NATURAL LABELED AMINO ACID AND METHOD OF CONSTRUCTING AMINO ACID/tRNA COMPLEX 未知
公开(公告)号：AU2003281613A1
公开(公告)日：2004-02-09
申请号：AU2003281613
申请日：2003-07-15
申请人： HOHSAKA TAKAHIRO , PROTEIN EXPRESS CO LTD
发明人： SISIDO MASAHIKO , HOHSAKA TAKAHIRO
IPC分类号： C07F5/02 , C09B57/00 , C09B57/10 , C07K1/13 , C07K16/40 , C12N15/09
摘要： This invention relates to: a labeled amino acid that can be introduced into a protein with the aid of a protein synthesis system; a functional protein having functions derived from a label compound; a labeled amino acid comprising an aromatic ring bound to an amino acid side chain and a label compound bound thereto via the aromatic ring; a functional protein to which the labeled amino acid has been introduced; and a novel method for effectively obtaining a labeled amino acid-tRNA complex.

10. 发明专利

BR9811442A 未知
公开(公告)号：BR9811442A
公开(公告)日：2002-01-15
申请号：BR9811442
申请日：1998-09-03
申请人： PROTEIN EXPRESS
发明人： HERTELENDY ZSOLT ISTVAN , WEINGER MURRAY
IPC分类号： A61K9/00 , A61K9/02 , A61K39/07 , A61K39/09 , A61K39/108 , A61K39/116 , A61K47/34 , A61P13/00 , A61P13/02 , A61P15/00 , A61P31/04

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