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1. 发明申请

WO2003070977A3 METHOD FOR DETECTING SINGLE NUCLEOTIDE POLYMORPHISMS 审中-公开
公开(公告)号：WO2003070977A3
公开(公告)日：2003-08-28
申请号：PCT/EP2003/001420
申请日：2003-02-13
申请人： NANOGEN RECOGNOMICS GMBH , KAPPEL, Andreas
发明人： KAPPEL, Andreas
IPC分类号： C12Q1/68
摘要： The present invention relates to a method which can be used for detecting mutations, in particular single nucleotide polymorphisms in parallel in different nucleotide sequences. The method comprises the procedural steps of hybridizing d(G M eATC) containing single-stranded sample nucleotide sequences with single 10 stranded reference nucleotide sequences, fixing single-stranded reference nucleotide sequences or single-stranded sample nucleotide sequences before or during the hybridization, or heteroduplexes consisting of reference and sample nucleotide sequences after or during the hybridization, on a surface, incubating with mutS, mutL and mutH for the incision of the complementary region of the is d(G Me ATC)-sequence in the reference nucleic acid sequence, if a mismatch is present in the hybridized reference and sample nucleic acid sequences, removing the 3`-ends of the incised reference nucleic acids and incubating with a DNA ploymerase and with labeled esoxynucleotidetriphosphates.

2. 发明申请

WO2003070977A2 METHOD FOR DETECTING SINGLE NUCLEOTIDE POLYMORPHISMS 审中-公开
标题翻译：检测单核苷酸多态性的方法
公开(公告)号：WO2003070977A2
公开(公告)日：2003-08-28
申请号：PCT/EP2003/001420
申请日：2003-02-13
申请人： NANOGEN RECOGNOMICS GMBH , KAPPEL, Andreas
发明人： KAPPEL, Andreas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2525/117 , C12Q2522/101 , C12Q2521/514
摘要： The present invention relates to a method which can be used for detecting mutations, in particular single nucleotide polymorphisms in parallel in different nucleotide sequences. The method comprises the procedural steps of hybridizing d(G M eATC) containing single-stranded sample nucleotide sequences with single 10 stranded reference nucleotide sequences, fixing single-stranded reference nucleotide sequences or single-stranded sample nucleotide sequences before or during the hybridization, or heteroduplexes consisting of reference and sample nucleotide sequences after or during the hybridization, on a surface, incubating with mutS, mutL and mutH for the incision of the complementary region of the is d(G Me ATC)-sequence in the reference nucleic acid sequence, if a mismatch is present in the hybridized reference and sample nucleic acid sequences, removing the 3`-ends of the incised reference nucleic acids and incubating with a DNA ploymerase and with labeled esoxynucleotidetriphosphates.
摘要翻译：本发明涉及一种可用于检测不同核苷酸序列中突变，特别是单核苷酸多态性并行的方法。该方法包括将含有单链样品核苷酸序列的dGMeATC与单个10个参考核苷酸序列杂交的步骤，在杂交之前或杂交期间固定单链参考核苷酸序列或单链样品核苷酸序列，或由参考和如果在杂交参考文献中存在错配，则在表面上或杂交后的样品核苷酸序列在表面上，用mutS mutL和mutH孵育用于切割引物核酸序列中的dGMeATC序列，样品核酸序列，去除切割的参考核酸的3'末端，并用DNA聚合酶和标记的二氧化三铁三磷酸酯孵育。

3. 发明申请

WO03066890A2 METHOD FOR DETERMINING THE METHYLATION PATTERN OF DNA 审中-公开
标题翻译： PROCEDURE用于确定DNA甲基化的图案
公开(公告)号：WO03066890A2
公开(公告)日：2003-08-14
申请号：PCT/EP0301035
申请日：2003-02-03
申请人： NANOGEN RECOGNOMICS GMBH , MUTH JOCHEN , KAPPEL ANDREAS , BEHRENSDORF HEIKE
发明人： MUTH JOCHEN , KAPPEL ANDREAS , BEHRENSDORF HEIKE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2565/513 , C12Q2523/125
摘要： The invention relates to a method for determining the methylation pattern in DNA-sequences whereby non-methylated cytosine in the DNA-sequence is chemically converted to uracil and the thus treated DNA-sequence is hybridised by a detector-DNA-sequence. The hybrids are fixed in a locally dissolved manner on a surface. Subsequently, the detection of guanine/uracil-mispairing occurs by using substances detected by said mispairings.
摘要翻译：其中空间分辨用于在DNA序列的甲基化模式的确定描述的混合被未被甲基化的DNA序列的胞嘧啶的方法被化学转化为尿嘧啶，并且将这样处理过的DNA序列杂交与检测DNA序列，固定到一个表面是。继检测使用这样的错配识别物质发生鸟嘌呤/尿嘧啶不匹配的。

4. 发明申请

WO2003016561A2 VERFAHREN ZUR HERSTELLUNG VON CNA 审中-公开
标题翻译：制备CNA的方法
公开(公告)号：WO2003016561A2
公开(公告)日：2003-02-27
申请号：PCT/EP2002/009044
申请日：2002-08-13
申请人： NANOGEN RECOGNOMICS GMBH , REUSCHLING, Dieter , MÜLLER-IBELER, Jochen , WAGNER, Thomas , KRUMM, Thomas , WERMUTH, Jochen , PIGNOT, Marc
发明人： REUSCHLING, Dieter , MÜLLER-IBELER, Jochen , WAGNER, Thomas , KRUMM, Thomas , WERMUTH, Jochen , PIGNOT, Marc
IPC分类号： C12Q1/68
CPC分类号： C07D239/47 , C07D239/54 , C07D473/00 , C07H21/00 , C07K7/06
摘要： Die Erfindung betrifft ein neues Verfahren zur Herstellung von CNA-Oligomeren sowie künstliche supramolekulare CNA-p-RNA-Paarungssysteme und deren Verwendung, insbesondere in biotechnologischen Assays.
摘要翻译：
本发明涉及一种新方法，CNA低聚物以及k导航使用人工超分子CNA-P-RNA配对的系统和它们的使用的制备中，特别是生物技术的测定。

5. 发明申请

WO02072595A3 METHOD FOR THE PRODUCTION OF PENTOPYRANOSYL NUCLEOSIDES 审中-公开
标题翻译：用于生产pentopyranosylnucleosides
公开(公告)号：WO02072595A3
公开(公告)日：2003-01-23
申请号：PCT/EP0202536
申请日：2002-03-07
申请人： NANOGEN RECOGNOMICS GMBH , KRETZSCHMAR GERHARD
发明人： KRETZSCHMAR GERHARD
IPC分类号： C07H19/06 , C07H19/16
CPC分类号： C07H19/06 , C07H19/16 , Y02P20/55
摘要： The invention relates to an improved method for the production of pentopyranosyl nucleosides, in which a significant improvement and simplification of the method steps described in DE-A-19741715 can be achieved. According to the invention, a migration of the 2' acyl protecting group, from the 2' position to the 3' position of the pentopyranoside can be achieved by the application of a catalyst of formula (IVa) or (IVb), where A = -CH2-, -NR -,R , H, optionally substituted alkyl, cycloalkyl, aryl or aralkyl, D = a group of formula CmH2m-, m = a whole number from 1 to 6, R , R and R independently = H, optionally substituted alkyl, cycloalkyl, aryl or aralkyl, or R and R together represent a -CoH2o- group, where o = a whole number from 2 to 4 and R = a NR R group, where R and R independently = H, optionally substituted alkyl, cycloalkyl, aryl or aralkyl.
摘要翻译：本发明涉及一种用于制备在其中所描述的方法步骤的改进显著和简化DE-A-19741715，可以实现pentopyranosylnucleosides的改进方法。利用本发明的方法中，所述2'-酰基保护基的迁移是由2'-位置到pentopyranoside的3'-位置，实现其中在式（IVa）或（IVb）中使用的催化剂。在该式中A-CH 2 - 或-NR <20> - ，R <20>为氢，烷基，环烷基，芳基或芳烷基，其任选地被取代的，D是下式基团的-CmH2m-和m是从1到的整数 6，R <18>，R <19>和R <24>彼此独立地，相同或不同的，是氢，烷基，环烷基，芳基或芳烷基，其任选被取代，或者R <18>和R <19>一起代表代表一组-CoH2o-，其中o为2至4的整数，并且R <21>表示基团-NR <22> - [R <23>，其中R <22>和R <23>彼此独立地，等于或不同，是氢，烷基，环烷基，芳基或芳烷基，其任选被取代。

6. 发明申请

US20040198966A1 Pentopyranosylnucleoside, its preparation and use 审中-公开
标题翻译： PentopyranosylNucleoside，其制备和用途
公开(公告)号：US20040198966A1
公开(公告)日：2004-10-07
申请号：US10644592
申请日：2003-08-19
申请人： Nanogen Recognomics GmbH
发明人： Christian Miculka , Norbert Windhab , Tilmann Brandstetter , Gerhard Burdinski
IPC分类号： C07K009/00 , C07H021/02
CPC分类号： C07H21/00 , A61K47/549 , A61K49/0013 , C07H19/04 , C07H21/02 , C07H21/04 , G01N33/532 , Y02P20/55 , Y10S977/728 , Y10S977/80 , Y10S977/836 , Y10S977/894 , Y10S977/896 , Y10S977/904 , Y10S977/914 , Y10S977/915 , Y10S977/924 , Y10S977/925
摘要： The invention relates to a pentopyranosylnucleoside of the formula (I) or of the formula (II) 1 their preparation and use for the production of a therapeutic, diagnostic and/or electronic component.
摘要翻译：本发明涉及式（I）或式（II）的戊吡喃糖基核苷及其制备和用于制备治疗，诊断和/或电子成分的方法。

7. 发明申请

WO2005059171A1 USE OF A MUTATION IN THE BRAF GENE FOR THE DETERMINATION OF THE MALIGNANCY OF MELANOMA CELLS 审中-公开
标题翻译：在BRAF基因中用于确定MELANOMA细胞恶性的用途
公开(公告)号：WO2005059171A1
公开(公告)日：2005-06-30
申请号：PCT/EP2004/013398
申请日：2004-11-26
申请人： NANOGEN RECOGNOMICS GMBH , KAPPEL, Andreas
发明人： KAPPEL, Andreas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/156
摘要： The invention relates to the use of oligonucleotide probes comprising exon 15 of the BRAF gene or a part thereof comprising codon 599 for the detection of the malignancy of melanoma cells.
摘要翻译：本发明涉及包含BRAF基因的外显子15或包含密码子599的部分的寡核苷酸探针用于检测黑素瘤细胞恶性肿瘤的用途。

8. 发明申请

WO2003066890A3 VERFAHREN ZUR BESTIMMUNG DES METHYLIERUNGSMUSTERS VON DNA 审中-公开
公开(公告)号：WO2003066890A3
公开(公告)日：2003-08-14
申请号：PCT/EP2003/001035
申请日：2003-02-03
申请人： NANOGEN RECOGNOMICS GMBH , MUTH, Jochen , KAPPEL, Andreas , BEHRENSDORF, Heike
发明人： MUTH, Jochen , KAPPEL, Andreas , BEHRENSDORF, Heike
IPC分类号： C12Q1/68
摘要： Beschrieben wird eine Verfahren zur Bestimmung des Methylierungsmusters in DNA-Sequenzen wobei nicht methyliertes Cytosin der DNA-Sequenz chemisch in Uracil umgewandelt wird und die so behandelte DNA-Sequenz mit einer Detektor-DNA-Sequenz hybridisiert wird, wobei die Hybride ortsaufgelöst auf einer Oberfläche fixiert sind. Im Anschluss erfolgt die Detektion von Guanin/Uracil-Fehlpaarungen unter Verwendung von solchen Fehlpaarungen erkennenden Substanzen.

9. 发明申请

WO2003008638A2 SORTING AND IMMOBILIZATION SYSTEM FOR NUCLEIC ACIDS USING SYNTHETIC BIDNING SYSTEMS 审中-公开
标题翻译：用合成排污系统分析核酸的分选和固定系统
公开(公告)号：WO2003008638A2
公开(公告)日：2003-01-30
申请号：PCT/EP2002/001532
申请日：2002-02-14
申请人： NANOGEN RECOGNOMICS GMBH , SCHWEITZER, Markus , ANDERSON, Richard , FIECHTNER, Michael , MÜLLER-IBELER, Jochen , RADDATZ, Stefan , BRÜCHER, Christoph , WINDHAB, Norbert , ORWICK, Jill , SCHNEIDER, Eberhard , PIGNOT, Marc , KIENLE, Stefan
发明人： SCHWEITZER, Markus , ANDERSON, Richard , FIECHTNER, Michael , MÜLLER-IBELER, Jochen , RADDATZ, Stefan , BRÜCHER, Christoph , WINDHAB, Norbert , ORWICK, Jill , SCHNEIDER, Eberhard , PIGNOT, Marc , KIENLE, Stefan
IPC分类号： C12Q1/68
CPC分类号： C07H21/00 , B01J19/0046 , B01J2219/00608 , B01J2219/00617 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00722 , C12Q1/6837 , G01N33/54353 , C12Q2565/518 , C12Q2525/117 , C12Q2525/113
摘要： The present invention relates to conjugates of synthetic binding units and nucleic acids. The present invention also relates to methods for sorting and immobilizing nucleic acids on support materials using such conjugates by specific molecular addressing of the nucleic acids mediated by the snythetic binding systems. Particularly, the present invention also relates to novel methods of utilizing conjugates of synthetic binding units and nucleic acids to in active electronic array systems to produce novel array constructs from the conjugates, and the use of such constructs in various nucleic acid assay formats. In addition, the present invention relates to various novel forms of such conjugates, improved methods of making solid phase synthesized conjugates, and improved methods of conjugating pre-synthesized synthetic binding units and nucleic acids. The present invention also relates to the use of conjugates of synthetic binding units and nucleic acids as substrates for various enzymatic reactions, including nucleic acid amplification reactions.
摘要翻译：本发明涉及合成结合单元和核酸的缀合物。本发明还涉及使用这样的缀合物通过由所述合成结合系统介导的核酸的特定分子寻址来将核酸分选和固定在载体材料上的方法。特别地，本发明还涉及利用合成结合单元和核酸的缀合物在活性电子阵列系统中从缀合物产生新阵列构建体的新方法，以及这些构建体在各种核酸测定形式中的用途。此外，本发明涉及这种缀合物的各种新形式，制备固相合成缀合物的改进方法，以及缀合预合成的合成结合单元和核酸的改进方法。本发明还涉及合成结合单元和核酸的缀合物作为各种酶促反应（包括核酸扩增反应）的底物的用途。

10. 发明申请

WO2002064825A2 METHODS, PROCEDURES, AND FORMATS FOR USING MICROELECTRONIC ARRAAY DEVICES TO PERFORM MULTIPLEX IMMUNOASSAY ANALYSES 审中-公开
标题翻译：使用微电子阵列设备进行多重免疫分析的方法，程序和格式
公开(公告)号：WO2002064825A2
公开(公告)日：2002-08-22
申请号：PCT/EP2002/001521
申请日：2002-02-14
申请人： NANOGEN RECOGNOMICS GMBH , WINDHAB, Norbert , HELLER, Michael , ANDERSON, Richard , FIETCHNER, Michael , HOPPE, Hans-Ulrich , NOVA, Tina , SUNDQUIST, Alfred , ORWICK, Jill , MÜLLER-IBELER, Jochen , ACKLEY, Donald
发明人： WINDHAB, Norbert , HELLER, Michael , ANDERSON, Richard , FIETCHNER, Michael , HOPPE, Hans-Ulrich , NOVA, Tina , SUNDQUIST, Alfred , ORWICK, Jill , MÜLLER-IBELER, Jochen , ACKLEY, Donald
IPC分类号： C12Q1/68
CPC分类号： G01N21/171 , B01J19/0046 , B01J2219/00653 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , C12Q1/6825 , C40B30/04 , C40B40/06 , C40B40/10 , G01N21/59 , G01N33/54366 , G01N33/6845 , G01N2800/52
摘要： This invention relates to devices and methods for carrying out multi-step and multiplex immunoaffinity binding reactions in microscopic formats. In particular, these devices and methods allow the user to rapidly carry out multiple immunoassays in the same sample volume, and to rapidly resolve the results of those immunoassays in an electronically assisted format. The assays may be further multiplexed in that several samples may be analyzed and visualized on the same microelectronic array. In addition, the methods and procedures of the invention allow the use of electronic stringency to further improve the specificity and accuracy of the immunoassays on the microelectronic array devices.
摘要翻译：本发明涉及以微观形式进行多步和多重免疫亲和结合反应的装置和方法。特别地，这些装置和方法允许用户在相同的样本体积中快速进行多次免疫测定，并以电子辅助格式快速地解析那些免疫测定的结果。可以进一步复合测定，因为可以在相同的微电子阵列上分析和可视化几个样品。此外，本发明的方法和程序允许使用电子严格性进一步提高微电子阵列器件上免疫测定的特异性和准确性。

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