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    • 2. 发明申请
    • ASSAY FOR DUROC MUSCLE FIBRE TYPE
    • DUROC MUSCLE纤维类型测定
    • WO1998015837A1
    • 1998-04-16
    • PCT/GB1997002741
    • 1997-10-07
    • MEAT AND LIVESTOCK COMMISSIONMALTIN, Charlotte, AnneSTEVEN, JohnWARKUP, Christopher, Charles
    • MEAT AND LIVESTOCK COMMISSION
    • G01N33/68
    • C12Q1/6876C12Q1/6804C12Q1/6827C12Q2600/124C12Q2600/156G01N33/6887
    • There is provided an assay to determine whether an animal has an allele for or exhibits muscle fibre composition characteristic of a Duroc pig. The assay comprises obtaining a tissue sample from said animal and subjecting said sample to genetic analysis and/or obtaining a muscle sample from said animal and determining by histochemical or immunochemical analysis whether said sample exhibits phenotypic traits indicative of said allele(s) and/or shows a muscle composition characterisitc to the Duroc pig. Suitable physical traits include the following: i. the percentage frequency of SO fibres present in said muscle; and/or ii. the number of SO fibres present per cluster; and/or iii. the number of muscle fibres present per cluster; and/or iv. the level of m calpain present per unit muscle; and/or v. the level of mu calpain present per unit muscle. The assay can be used to select animals having Duroc muscle type for breeding or for meat production. Anti-calpain antibodies are especially useful and may be employed as part of an ELISA style assay.
    • 提供了一种用于确定动物是否具有Duroc猪特征的等位基因或表现出肌肉纤维组成的测定法。 该测定包括从所述动物获得组织样品,并对所述样品进行遗传分析和/或从所述动物获得肌肉样品,并通过组织化学或免疫化学分析来确定所述样品是否表现出表达所述等位基因的表型性状和/或 显示了杜罗克猪的肌肉组成特征。 合适的身体特征包括:i。 所述肌肉中存在的SO纤维的频率百分比; 和/或ii。 每个簇存在的SO纤维的数量; 和/或iii。 每簇存在肌肉纤维数量; 和/或iv。 每单位肌肉中存在的钙蛋白酶水平; 和/或v。每单位肌肉中存在的μ钙蛋白酶的水平。 该测定可用于选择具有Duroc肌肉类型的动物用于育种或用于肉类生产。 抗钙蛋白酶抗体是特别有用的,可用作ELISA样式测定的一部分。