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1. 发明授权

US5770417A Three-dimensional fibrous scaffold containing attached cells for producing vascularized tissue in vivo 失效
标题翻译：三维纤维支架含有用于在体内产生血管化组织的附着细胞
公开(公告)号：US5770417A
公开(公告)日：1998-06-23
申请号：US203705
申请日：1994-02-28
申请人： Joseph P. Vacanti , Robert S. Langer
发明人： Joseph P. Vacanti , Robert S. Langer
IPC分类号： C12N5/06 , A61F2/06 , A61L26/00 , A61L27/00 , A61L27/38 , C12N5/00 , C12N11/08 , A61F2/28 , A61F2/18
CPC分类号： A61L27/3839 , A61F2/062 , A61L27/38 , C12N5/0068 , A61L2430/06 , A61L2430/28 , C12N2501/18 , C12N2533/30 , C12N2533/40
摘要： A cell-scaffold composition is prepared in vitro for implanting to produce functional organ tissue in vivo. The scaffold is three-dimensional and is composed of hollow or solid fibers of a biocompatible, synthetic polymer which is biodegradable or non-biodegradable. The fibers of the scaffold may have a branched configuration extending outwardly from a central stem. Fibers of the scaffold are spaced apart such that the maximum distance over which diffusion of nutrients and gases must occur through a mass of cells attached to the fibers is between 200 and 300 microns. The diffusion provides free exchange of nutrients, gases and waste to and from cells proliferating throughout the scaffold in an amount effective to maintain cell viability throughout the scaffold in the absence of vascularization. Cells derived from vascularized organ tissue are attached in vitro to the surface of the fibers uniformly throughout the scaffold in an amount effective to produce functional vascularized organ tissue in vivo, the cells are grown on the scaffold in a nutrient solution in vitro to form the cell-scaffold composition which is implanted in a host at a location having adequate vascularization to allow growth of blood vessels into the cell-scaffold composition. Growth factors, compounds stimulating angiogenesis and immunomodulators may be provided in the cell-scaffold composition and the fibers may have a coating to enhance cell attachment. Combinations of cell-scaffold compositions containing different cell populations may be implanted.
摘要翻译：体外制备细胞支架组合物用于植入以在体内产生功能性器官组织。支架是三维的，并且由可生物降解或不可生物降解的生物相容的合成聚合物的中空或固体纤维组成。支架的纤维可以具有从中心杆向外延伸的分支构型。支架的纤维间隔开，使得营养物和气体的扩散必须通过连接到纤维上的大量细胞发生的最大距离在200和300微米之间。该扩散提供了在整个支架中增殖的细胞和来自增殖细胞的营养物质，气体和废物的有效量，以在不存在血管形成的情况下维持整个支架的细胞活力。从血管化的器官组织衍生的细胞在体外通过整个支架均匀地连接到纤维表面，其数量有效地在体内产生功能性血管化的器官组织，细胞在体外在营养溶液中在支架上生长以形成细胞脚手架组合物，其植入在具有足够血管形成的位置的宿主中，以允许血管生长进入细胞 - 支架组合物。可以在细胞支架组合物中提供生长因子，刺激血管发生的化合物和免疫调节剂，并且纤维可以具有增强细胞附着的包衣。可以植入含有不同细胞群的细胞支架组合物。

2. 发明授权

US5770193A Preparation of three-dimensional fibrous scaffold for attaching cells to produce vascularized tissue in vivo 失效
标题翻译：制备用于附着细胞以在体内产生血管化组织的三维纤维支架
公开(公告)号：US5770193A
公开(公告)日：1998-06-23
申请号：US203521
申请日：1994-02-28
申请人： Joseph P. Vacanti , Robert S. Langer
发明人： Joseph P. Vacanti , Robert S. Langer
IPC分类号： C12N5/06 , A61F2/06 , A61L26/00 , A61L27/00 , A61L27/38 , C12N5/00 , C12N11/08 , A61F2/28 , A61F2/18
CPC分类号： A61L27/3839 , A61F2/062 , A61L27/38 , C12N5/0068 , A61L2430/06 , A61L2430/28 , C12N2501/18 , C12N2533/30 , C12N2533/40
摘要： Fibers of a biocompatible, biodegradable or non-biodegradable, synthetic polymer are provided, and are formed into a three-dimensional scaffold. The fibers of the scaffold may have a branched configuration extending outwardly from a central stem. The fibers provide sufficient surface area to permit attachment to the scaffold in vitro of an amount of cells effective to produce functional vascularized organ tissue in vivo. Fibers of the scaffold are spaced apart such that the maximum distance over which diffusion of nutrients and gases must occur through a mass of cells attached to the fibers is between 200 and 300 microns. The diffusion provides free exchange of nutrients, gases and waste to and from cells proliferating throughout the scaffold in an amount effective to maintain cell viability throughout the scaffold in the absence of vascularization. Cells derived from vascularized organ tissue are attached in vitro to the surface of the fibers uniformly throughout the scaffold and the cells are grown on the scaffold in a nutrient solution in vitro to form a cell-scaffold composition. Growth factors, compounds stimulating angiogenesis and immunomodulators may be provided in the cell-scaffold composition and the fibers may have a coating to enhance cell attachment. The cell-scaffold composition is implanted in a host at a location having adequate vascularization to allow growth of blood vessels into the cell-scaffold composition. Combinations of cell-scaffold compositions containing different cell populations may be implanted.
摘要翻译：提供生物相容性，可生物降解或不可生物降解的合成聚合物的纤维，并形成三维支架。支架的纤维可以具有从中心杆向外延伸的分支构型。纤维提供足够的表面积，以允许体外附着到支架体内的有效量的能在体内产生功能性血管化器官组织的细胞。支架的纤维间隔开，使得营养物和气体的扩散必须通过连接到纤维上的大量细胞发生的最大距离在200和300微米之间。该扩散提供了在整个支架中增殖的细胞和来自增殖细胞的营养物质，气体和废物的有效量，以在不存在血管形成的情况下维持整个支架的细胞活力。源自血管化器官组织的细胞在整个支架中均匀地附着在纤维表面上，并且细胞在体外在营养溶液中在支架上生长以形成细胞 - 支架组合物。可以在细胞支架组合物中提供生长因子，刺激血管发生的化合物和免疫调节剂，并且纤维可以具有增强细胞附着的包衣。将细胞 - 支架组合物植入到具有足够血管形成的位置的宿主中，以允许血管生长进入细胞 - 支架组合物。可以植入含有不同细胞群的细胞支架组合物。

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