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1. 发明申请

WO2019210233A1 REAGENTS AND METHODS FOR ELEMENTAL MASS SPECTROMETRY OF BIOLOGICAL SAMPLES 审中-公开
公开(公告)号：WO2019210233A1
公开(公告)日：2019-10-31
申请号：PCT/US2019/029443
申请日：2019-04-26
申请人： FLUIDIGM CANADA INC. , FLUIDIGM CORPORATION , ZABINYAKOV, Nick
发明人： ZABINYAKOV, Nick , LOBODA, Alexander V. , ORNATSKY, Olga , BARANOV, Vladimir
IPC分类号： C12M1/00 , C12M1/34 , G01N35/00 , G01N35/10 , H01J49/00 , H01J49/02
摘要： Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.

2. 发明申请

WO2018013723A1 SINGLE-CELL TRANSCRIPT SEQUENCING 审中-公开
标题翻译：单细胞转录测序
公开(公告)号：WO2018013723A1
公开(公告)日：2018-01-18
申请号：PCT/US2017/041770
申请日：2017-07-12
申请人： FLUIDIGM CORPORATION
发明人： LIVAK, Kenneth J. , FEKETE, Richard A.
IPC分类号： C12Q1/68
摘要： Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.
摘要翻译：本文描述了用于制备用于来自细胞群的RNA的单细胞转录物测序的DNA模板的方法。该方法需要将来自群体的细胞分布到单独的反应体积中，使得多个单独的反应体积各自含有单个分离的细胞，其中细胞在分布之前已经用固定剂处理。然后使分离的细胞透化或破碎，并通过反转录制备cDNA，然后扩增。还提供了用于从单细胞中的T细胞受体或免疫球蛋白有效生成DNA模板的新化学物质。

3. 发明申请

WO2016077770A2 IMAGING MASS CYTOMETRY USING MOLECULAR TAGGING 审中-公开
标题翻译：用分子标记成像质量细胞计数
公开(公告)号：WO2016077770A2
公开(公告)日：2016-05-19
申请号：PCT/US2015/060706
申请日：2015-11-13
申请人： FLUIDIGM CANADA INC. , FLUIDIGM CORPORATION
发明人： LOBODA, Alexander V.
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/58 , G01N2458/15
摘要： Methods of imaging a biological sample by mass cytometry using molecular tagging are disclosed.
摘要翻译：公开了使用分子标签通过质量细胞计量术对生物样品进行成像的方法。

4. 发明申请

WO2016011177A1 REAGENTS AND KIT COMPOSITIONS FOR SINGLE-CELL WHOLE GENOME AMPLIFICATION 审中-公开
标题翻译：用于单细胞全基因扩增的试剂和试剂盒组合物
公开(公告)号：WO2016011177A1
公开(公告)日：2016-01-21
申请号：PCT/US2015/040611
申请日：2015-07-15
申请人： FLUIDIGM CORPORATION
发明人： CHEN, Peilin , WANG, Jing , MAY, Andrew
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12P19/34 , C12Q1/6846 , C12Q1/6848 , C12Q2525/113 , C12Q2525/179 , C12Q2527/101 , C12Q2527/125 , C12Q2531/119
摘要： The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.
摘要翻译：本发明提供使用Phi 29 DNA聚合酶的单细胞全基因组扩增的试剂，试剂盒和方法。

5. 发明申请

WO2012162267A2 NUCLEIC ACID ENCODING REACTIONS 审中-公开
标题翻译：核酸编码反应
公开(公告)号：WO2012162267A2
公开(公告)日：2012-11-29
申请号：PCT/US2012/038894
申请日：2012-05-21
申请人： FLUIDIGM CORPORATION , ANDERSON, Megan , CHEN, Peilin , FOWLER, Brian , JONES, Robert C. , KAPER, Fiona , LEBOFSKY, Ronald , MAY, Andrew
发明人： ANDERSON, Megan , CHEN, Peilin , FOWLER, Brian , JONES, Robert C. , KAPER, Fiona , LEBOFSKY, Ronald , MAY, Andrew
IPC分类号： C40B20/00 , C40B50/06 , C40B40/06 , C40B30/04 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6855 , C12N15/10 , C12N15/1065 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
摘要： Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.
摘要翻译：本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列，一个或多个靶核苷酸序列。在具体实施方案中，产生具有适配器（例如适合用于高通量DNA测序）的核酸片段。在其它实施方案中，关于反应混合物的信息被编码成反应产物。本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。在具体实施方案中，本发明的方法还需要进行双向DNA测序。本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

6. 发明申请

WO2011053790A2 ASSAY OF CLOSELY LINKED TARGETS IN FETAL DIAGNOSIS AND COINCIDENCE DETECTION ASSAY FOR GENETIC ANALYSIS 审中-公开
标题翻译：用于遗传分析的肛门诊断和联合检测测定中的关联目标的测定
公开(公告)号：WO2011053790A2
公开(公告)日：2011-05-05
申请号：PCT/US2010/054728
申请日：2010-10-29
申请人： FLUIDIGM CORPORATION , ZIMMERMANN, Bernhard Georg , UNGER, Marc
发明人： ZIMMERMANN, Bernhard Georg , UNGER, Marc
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2545/114
摘要： Methods for detecting chromosomal aneuploidy of a specified chromosome or chromosome region are provided. Also provided are methods for genetic analysis of heterogeneously sized chromosomal DNA fragments. The methods are useful for non-invasive prenatal diagnosis and other genetic analyses.
摘要翻译：提供了检测特定染色体或染色体区域染色体非整倍性的方法。还提供了用于遗传分析不均匀大小的染色体DNA片段的方法。该方法可用于非侵入性产前诊断和其他遗传分析。

7. 发明申请

WO2010115154A1 MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 审中-公开
标题翻译：用于靶向靶核酸的多重PCR扩增方法
公开(公告)号：WO2010115154A1
公开(公告)日：2010-10-07
申请号：PCT/US2010/029854
申请日：2010-04-02
申请人： FLUIDIGM CORPORATION , MAY, Andrew , CHEN, Peilin , WANG, Jun , KAPER, Fiona , ANDERSON, Megan
发明人： MAY, Andrew , CHEN, Peilin , WANG, Jun , KAPER, Fiona , ANDERSON, Megan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
摘要： In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
摘要翻译：在某些实施方案中，本发明提供了其中将核苷酸标签和任选的条形码核苷酸序列加入靶核苷酸序列的扩增方法。在其它实施例中，本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。微流体装置还包括多组第一腔室和多组第二腔室。每组第一腔室与多个第一输入管线中的一个流体连通。每组第二腔室与多个第二输入管线中的一个流体连通。微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。

8. 发明申请

WO2005121864A9 OPTICAL LENS SYSTEM AND METHOD FOR MICROFLUIDIC DEVICES 审中-公开
公开(公告)号：WO2005121864A9
公开(公告)日：2005-12-22
申请号：PCT/US2005/020250
申请日：2005-06-07
申请人： FLUIDIGM CORPORATION , UNGER, Marc, A.
发明人： UNGER, Marc, A.
IPC分类号： H01L27/00 , G01T1/10 , G01N21/00 , G01N21/64 , H05B33/00 , G01J3/30 , C12Q1/00
摘要： An apparatus for imaging one or more selected fluorescence indications from a microfluidic device (205). The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device (205). The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device (205). The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system (210, 212) coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

9. 发明申请

WO2005011947A2 IMAGE PROCESSING METHOD AND SYSTEM FOR MICROFLUIDIC DEVICES 审中-公开
标题翻译：图像处理方法和微流体装置系统
公开(公告)号：WO2005011947A2
公开(公告)日：2005-02-10
申请号：PCT/US2004/024591
申请日：2004-07-28
申请人： FLUIDIGM CORPORATION , TAYLOR, Colin Jon , SUN, Gang , DUBE, Simant
发明人： TAYLOR, Colin Jon , SUN, Gang , DUBE, Simant
IPC分类号： B29C
CPC分类号： G06K9/36 , B01L3/5027 , G06K9/00 , G06K9/00127 , G06K9/00134 , G06K9/6232 , G06T7/0008 , G06T7/0012 , G06T7/254 , G06T7/33 , G06T2207/30004
摘要： A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.
摘要翻译：一种用于处理微流体装置的图像的方法。该方法包括接收微流体装置的第一图像。第一图像对应于第一状态。另外，该方法包括接收微流体装置的第二图像。第二图像对应于第二状态。此外，该方法包括将第一图像和第二图像变换为第三坐标空间。此外，该方法包括至少基于与变换的第一图像和变换的第二图像相关联的信息获得第三图像，并且处理第三图像以获得与第一状态和第二状态相关联的信息。

10. 发明申请

WO2004103563A3 METHOD AND SYSTEM FOR MICROFLUIDIC DEVICE AND IMAGING THEREOF 审中-公开
公开(公告)号：WO2004103563A3
公开(公告)日：2004-12-02
申请号：PCT/US2004/016115
申请日：2004-05-20
申请人： FLUIDIGM CORPORATION , QUAN, Emerson Chueng , TAYLOR, Colin, Jon , LEE, Michael , CEASAR, Christopher, G. , HARRIS, Greg , SUN, Gang
发明人： QUAN, Emerson Chueng , TAYLOR, Colin, Jon , LEE, Michael , CEASAR, Christopher, G. , HARRIS, Greg , SUN, Gang
IPC分类号： B29D9/00
摘要： A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

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