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1. 发明申请

WO2007081772A9 TAXUS TRANSFORMATION, TRANSFORMED CELLS, AND RELATED COMPOSITIONS AND METHODS 审中-公开
标题翻译：红豆杉转化，转化细胞以及相关组合物和方法
公开(公告)号：WO2007081772A9
公开(公告)日：2007-08-30
申请号：PCT/US2007000222
申请日：2007-01-03
申请人： EXELIXIS PLANT SCIENCES INC , MATHEWS HELENA V , REDDY VAKA S , LAMMERS ALLAN H
发明人： MATHEWS HELENA V , REDDY VAKA S , LAMMERS ALLAN H
IPC分类号： C12N15/82
CPC分类号： C12N15/8205
摘要： Reported herein are methods for obtaining stably transformed callus in Taxus media 'Hicksii', including particularly methods that involve using needles, stem, or bark peel as explant material for transformation. Also provided are descriptions of several promoter activities in directing reporter gene expression in Taxus media cells, in particular cells in suspension cultures, callus and needles. Transgenic plants (e.g., Taxus plants), plant cells, cell lines, and tissues (including seeds) are also provided, in particular those that express one or more enzymes in a paclitaxel biosynthesis pathway.
摘要翻译：本文报道了用于获得红豆杉培养基“希克西”中稳定转化的愈伤组织的方法，特别包括涉及使用针，茎或树皮作为外植体材料进行转化的方法。还提供了指导报道基因在Taxus培养基细胞中的几种启动子活性的描述，特别是悬浮培养物，愈伤组织和针头中的细胞。还提供了转基因植物（例如，红豆杉属植物），植物细胞，细胞系和组织（包括种子），特别是在紫杉醇生物合成途径中表达一种或多种酶的那些。

2. 发明申请

WO2010080530A2 PLANT CELL CULTURE FOR PRODUCTION OF NATURAL PRODUCTS WITH REDUCED GLUCOSINOLATE CONTAMINATION 审中-公开
标题翻译：植物细胞培养生产天然产物，降低葡萄糖甙的污染
公开(公告)号：WO2010080530A2
公开(公告)日：2010-07-15
申请号：PCT/US2009068559
申请日：2009-12-17
申请人： EXELIXIS PLANT SCIENCES INC , MATHEWS HELENA V , YOON SUNG-YONG , VENKATRAMESH MYLAVARAPU
发明人： MATHEWS HELENA V , YOON SUNG-YONG , VENKATRAMESH MYLAVARAPU
IPC分类号： C12N5/04 , A01H5/00 , C12N5/02
CPC分类号： C12P17/06 , A01H4/00 , C12N5/04
摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.
摘要翻译：公开了用于从十字花科植物获得具有降低的硫代葡萄糖苷酸污染的天然产物制剂的方法。所述方法可以包括从能够生产期望的天然产物的植物中培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织并且在液体培养基中培养所选择的愈伤组织。该方法还可以包括从培养物中回收天然产物。还公开了获得具有降低的硫代葡萄糖苷酸污染的卷心菜花青素的方法。所述方法可包括在液体培养基中培养具有减少的硫代葡萄糖苷产生的红球甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。该方法还可以包括从培养物中回收具有减少的硫代葡萄糖苷酸污染的花青素。最后，描述了几种特定的低芥子油苷细胞系。

3. 发明申请

WO2004096994A2 GENES UPREGULATED IN A TOMATO PLANT HAVING AN INCREASED ANTHOCYANIN CONTENT PHENOTYPE 审中-公开
标题翻译：在具有增加的蒽醌内含物的TOMATO植物中上调的基因
公开(公告)号：WO2004096994A2
公开(公告)日：2004-11-11
申请号：PCT/US2004/012826
申请日：2004-04-26
申请人： EXELIXIS PLANT SCIENCES, INC. , CLENDENNEN, Stephanie, K. , LIGHTNER, Jonathan
发明人： CLENDENNEN, Stephanie, K. , LIGHTNER, Jonathan
IPC分类号： C12N
CPC分类号： C12N15/825 , C07K14/415 , C12N9/90 , C12N15/8243
摘要： Tomato anthocyanin vacuolar transporter ("MTP77") and chalcone isomerase ("MTP96") are up-regulated in tomato plants that overexpress the ANT1 gene. Plant transformation vectors comprising isolated MTP77 or MTP96 polynucleotides can be made to generate transgenic plants having increased anthocyanin content relative to control plants.
摘要翻译：番茄花青素液泡转运蛋白（“MTP77”）和查耳酮异构酶（“MTP96”）在过表达ANT1基因的番茄植物中上调。可以制备包含分离的MTP77或MTP96多核苷酸的植物转化载体以产生相对于对照植物具有增加的花青素含量的转基因植物。

4. 发明申请

WO2004013301A2 SEED-ASSOCIATED PROMOTER SEQUENCES 审中-公开
标题翻译： SEED相关推广者序列
公开(公告)号：WO2004013301A2
公开(公告)日：2004-02-12
申请号：PCT/US0324330
申请日：2003-08-01
申请人： EXELIXIS PLANT SCIENCES INC , CLENDENNEN STEPHANIE K , LIGHTNER JONATHAN , SCHUSTER DEBRA K
发明人： CLENDENNEN STEPHANIE K , LIGHTNER JONATHAN , SCHUSTER DEBRA K
IPC分类号： C07K14/415 , C12N15/82 , C12N
CPC分类号： C12N15/8234 , C07K14/415 , C12N15/8247
摘要： Plant promoters that exhibit seed-associated expression are described, including bi-directional promoters capable of directing seed-associated expression in either orientation.
摘要翻译：描述了展示种子相关表达的植物启动子，包括能够以任一取向指导种子相关表达的双向启动子。

5. 发明申请

WO2003084312A2 IDENTIFICATION AND CHARACTERIZATION OF AN ANTHOCYANIN MUTANT (ANT1) IN TOMATO 审中-公开
标题翻译：苯甲酰胺突变体（ANT1）在TOMATO中的鉴定和表征
公开(公告)号：WO2003084312A2
公开(公告)日：2003-10-16
申请号：PCT/US2003/010369
申请日：2003-04-04
申请人： EXELIXIS PLANT SCIENCES, INC. , CONNORS, Karin, A. , MATHEWS, Helena, V. , LIU, Xing, Liang , CALDWELL, Colby, G.
发明人： CONNORS, Karin, A. , MATHEWS, Helena, V. , LIU, Xing, Liang , CALDWELL, Colby, G.
IPC分类号： A01H
CPC分类号： C07K14/415 , C12N15/8212 , C12N15/825
摘要： Flavonoids are obtained from plants that overexpress an ANT1 gene compared to wild-type plants. The plant may be a transgenic plant that contains a transformation vector that causes the overexpression of ANT1 . Alternatively, the plant can be selectively bred to have an allele of or mutation in an endogenous ANT1 gene that causes the overexpression of ANT1 compared to plants lacking the allele or mutation.
摘要翻译：与野生型植物相比，黄酮类化合物从过表达ANT1基因的植物获得。植物可以是含有导致ANT1的过表达的转化载体的转基因植物。或者，与缺乏等位基因或突变的植物相比，植物可以选择性繁殖以具有导致ANT1的过表达的内源性ANT1基因的等位基因或突变。

6. 发明申请

WO0183697A3 SYSTEM FOR FUNCTIONAL GENE DISCOVERY IN PLANTS 审中-公开
标题翻译：植物功能基因发现系统
公开(公告)号：WO0183697A3
公开(公告)日：2002-05-23
申请号：PCT/US0113886
申请日：2001-05-01
申请人： EXELIXIS PLANT SCIENCES INC
发明人： LIU ALEX , WADSWORTH GEORGE , MATHEWS HELENA , WAGNER RY , VAN WINKLE JILL , PETERS SANDRA , CLENDENNEN STEPHANIE
IPC分类号： A01H1/04 , C07K14/415 , C12N15/82 , G06F17/30 , G06F17/60
CPC分类号： C12N15/8216 , A01H1/04 , C07K14/415 , C12N15/8209 , C12N15/8213 , C12N15/8241 , Y10S707/99943
摘要： The invention is directed to a method of multigenerational analysis of plants modified by insertional mutagenesis, and a method for associating plant mutant trait and genotype information. The invention is further directed to a method for managing data pertaining to plant mutant trait and genotype information in a database. The invention is further directed to a system for managing plant mutant trait and genotype information in a database, a system for allowing users to associate plant trait and genotype information, a system for facilitating business transactions with a user regarding plant materials or a gene sequence of interest to a user and a computer-readable medium embodying a program of instructions for execution by a computer for implementing a system for allowing users to associate plant trait and genotype information.
摘要翻译：本发明涉及通过插入诱变修饰的植物的多代分析方法，以及将植物突变特征和基因型信息相关联的方法。本发明还涉及一种用于管理与数据库中植物突变特征和基因型信息有关的数据的方法。本发明还涉及用于管理数据库中的植物突变特征和基因型信息的系统，允许用户关联植物性状和基因型信息的系统，用于促进与用户的关于植物材料或基因序列的基因序列的系统对用户和计算机可读介质感兴趣，该介质包含用于由计算机执行以执行允许用户关联植物特征和基因型信息的系统的指令程序。

7. 发明申请

WO2007081772A3 TAXUS TRANSFORMATION, TRANSFORMED CELLS, AND RELATED COMPOSITIONS AND METHODS 审中-公开
公开(公告)号：WO2007081772A3
公开(公告)日：2007-07-19
申请号：PCT/US2007/000222
申请日：2007-01-03
申请人： EXELIXIS PLANT SCIENCES, INC. , MATHEWS, Helena, V. , REDDY, Vaka, S. , LAMMERS, Allan, H.
发明人： MATHEWS, Helena, V. , REDDY, Vaka, S. , LAMMERS, Allan, H.
IPC分类号： C12N15/82 , A01H5/00 , C12N5/10
摘要： Reported herein are methods for obtaining stably transformed callus in Taxus media 'Hicksii', including particularly methods that involve using needles, stem, or bark peel as explant material for transformation. Also provided are descriptions of several promoter activities in directing reporter gene expression in Taxus media cells, in particular cells in suspension cultures, callus and needles. Transgenic plants (e.g., Taxus plants), plant cells, cell lines, and tissues (including seeds) are also provided, in particular those that express one or more enzymes in a paclitaxel biosynthesis pathway.

8. 发明申请

WO2004096994A3 GENES UPREGULATED IN A TOMATO PLANT HAVING AN INCREASED ANTHOCYANIN CONTENT PHENOTYPE 审中-公开
标题翻译：在具有增加的蒽醌内含物的TOMATO植物中上调的基因
公开(公告)号：WO2004096994A3
公开(公告)日：2005-12-15
申请号：PCT/US2004012826
申请日：2004-04-26
申请人： EXELIXIS PLANT SCIENCES INC , CLENDENNEN STEPHANIE K , LIGHTNER JONATHAN
发明人： CLENDENNEN STEPHANIE K , LIGHTNER JONATHAN
IPC分类号： A01H5/00 , C07K14/415 , C12N20060101 , C12N9/90 , C12N15/00 , C12N15/05 , C12N15/09 , C12N15/29 , C12N15/52 , C12N15/82
CPC分类号： C12N15/825 , C07K14/415 , C12N9/90 , C12N15/8243
摘要： Tomato anthocyanin vacuolar transporter ("MTP77") and chalcone isomerase ("MTP96") are up-regulated in tomato plants that overexpress the ANT1 gene. Plant transformation vectors comprising isolated MTP77 or MTP96 polynucleotides can be made to generate transgenic plants having increased anthocyanin content relative to control plants.
摘要翻译：番茄花青素液泡转运蛋白（“MTP77”）和查耳酮异构酶（“MTP96”）在过表达ANT1基因的番茄植物中上调。可以制备包含分离的MTP77或MTP96多核苷酸的植物转化载体以产生相对于对照植物具有增加的花青素含量的转基因植物。

9. 发明申请

WO2003084312A3 IDENTIFICATION AND CHARACTERIZATION OF AN ANTHOCYANIN MUTANT (ANT1) IN TOMATO 审中-公开
公开(公告)号：WO2003084312A3
公开(公告)日：2003-10-16
申请号：PCT/US2003/010369
申请日：2003-04-04
申请人： EXELIXIS PLANT SCIENCES, INC. , CONNORS, Karin, A. , MATHEWS, Helena, V. , LIU, Xing, Liang , CALDWELL, Colby, G.
发明人： CONNORS, Karin, A. , MATHEWS, Helena, V. , LIU, Xing, Liang , CALDWELL, Colby, G.
IPC分类号： A01H1/00
摘要： Flavonoids are obtained from plants that overexpress an ANT1 gene compared to wild-type plants. The plant may be a transgenic plant that contains a transformation vector that causes the overexpression of ANT1 . Alternatively, the plant can be selectively bred to have an allele of or mutation in an endogenous ANT1 gene that causes the overexpression of ANT1 compared to plants lacking the allele or mutation.

10. 发明申请

WO0144285A2 DEVELOPMENT OF RESISTANCE TO RASPBERRY BUSHY DWARF VIRUS 审中-公开
标题翻译：发展RASPBERRY BUSHY DWARF病毒的抗性
公开(公告)号：WO0144285A2
公开(公告)日：2001-06-21
申请号：PCT/US0034188
申请日：2000-12-15
申请人： EXELIXIS PLANT SCIENCES INC , U S DEPT OF AGRICULTURE
发明人： MARTIN ROBERT R , MATHEWS HELENA , KELLER KAREN , KELLOGG JILL A , WAGNER RY
IPC分类号： C07K14/08 , C12N15/82 , C07K14/00
CPC分类号： C07K14/005 , C12N15/8283 , C12N2770/00022
摘要： The present invention relates to isolated Raspberry Bushy Dwarf Virus (RBDV) nucleic acid sequences which encode RBDV coat and movement proteins or polypeptides and mutant or modified forms thereof. The invention further relates to heterologous nucleic acid constructs, vectors, transformation methods, plant cells and plants comprising such RBDV-encoding nucleic acids and methods for inducing resistance to RBDV by transforming plants with a nucleic acid construct comprising RBDV protein or polypeptide-encoding nucleic acid sequences.
摘要翻译：本发明涉及编码RBDV外壳和运动蛋白或多肽及其突变体或修饰形式的分离的覆盆子丛生矮病毒（RBDV）核酸序列。本发明进一步涉及异源核酸构建体，载体，转化方法，植物细胞和包含这种编码RBDV的核酸的植物和通过用包含RBDV蛋白或编码多肽的核酸的核酸构建体转化植物来诱导对RBDV的抗性的方法序列。

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