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    • 5. 发明申请
    • MULTI-CHAMBERED CELL CULTURE DEVICE TO MODEL ORGAN MICROPHYSIOLOGY
    • 多室细胞培养装置模型有机微生物学
    • WO2014085498A1
    • 2014-06-05
    • PCT/US2013/072081
    • 2013-11-26
    • CFD RESEARCH CORPORATION
    • PRABHAKARPANDIAN, BalabhaskarPANT, Kapil
    • C12M3/00
    • C12M21/08C12M23/20C12M23/26C12M23/58C12M25/06C12M29/00C12M29/04C12M41/48
    • A cell culture device can include: a top wall; a bottom wall; one or more perimeter walls coupled with and extending between the top wall and bottom wall; and at least 3 distinct chambers between the top wall, bottom wall. The one or more perimeter walls can include: an internal chamber defined by at least one porous internal wall and having an internal chamber inlet and an internal chamber outlet; one or more boundary layer chambers having at least an inner boundary layer chamber defined by the at least one porous internal wall and at least one porous inner boundary layer wall, the at least one porous internal wall having a plurality of pores fluidically coupling the central internal chamber to the one or more boundary layer chamber; and an outer chamber defined by an outer porous boundary layer wall of the at least one porous boundary layer walls and the one or more perimeter walls and having an outer chamber inlet and an outer chamber outlet, the outer porous boundary layer wall having a plurality of pores that fluidically couple the outer chamber with the one or more boundary layer chambers. In one aspect, wherein the at least three distinct chambers are nonlinear and/or idealized.
    • 细胞培养装置可以包括:顶壁; 底壁 在顶壁和底壁之间耦合并延伸的一个或多个周边壁; 以及在顶壁,底壁之间的至少3个不同的腔室。 一个或多个周边壁可以包括:由至少一个多孔内壁限定并具有内部室入口和内部室出口的内部室; 一个或多个边界层室具有至少一个由所述至少一个多孔内壁和至少一个多孔内边界层壁限定的内边界层室,所述至少一个多孔内壁具有多个孔, 室到一个或多个边界层室; 以及由所述至少一个多孔边界层壁和所述一个或多个周边壁的外部多孔边界层壁限定的外部室,并且具有外部室入口和外部室出口,所述外部多孔边界层壁具有多个 将外室与一个或多个边界层室流体耦合的孔。 在一个方面,其中所述至少三个不同的室是非线性和/或理想化的。