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1. 发明申请

WO1990009436A1 A THERMOSTABLE (1,3-1,4)-'beta'-GLUCANASE 审中-公开
标题翻译：热稳定剂（1,3-1,4）-β-GLUCANASE
公开(公告)号：WO1990009436A1
公开(公告)日：1990-08-23
申请号：PCT/DK1990000044
申请日：1990-02-16
申请人： CARLSBERG A/S , AKADEMIE DER WISSENSCHAFTEN DER DDR , BORRISS, Rainer; , HOFEMEISTER, Jürgen; , THOMSEN, Karl, Kristian , OLSEN, Ole , VON WETTSTEIN, Dietrich
发明人： CARLSBERG A/S , AKADEMIE DER WISSENSCHAFTEN DER DDR
IPC分类号： C12N09/42
CPC分类号： C12Y302/01006 , A23K20/189 , C07K2319/00 , C07K2319/02 , C12N9/244 , C12N15/8242
摘要： Novel hybrid thermostable (1,3-1,4)- beta -glucanases, their use in food manufacturing and feed manufacturing, DNA fragments encoding such glucanases, organisms expressing the DNA fragments and a method for producing the thermostable (1,3-1,4)- beta -glucanases. Hybrid fusion genes encoding Bacillus (1,3-1,4)- beta -glucanases were constructed, the gene products of which are more thermostable than any (1,3-1,4)- beta -glucanase known until now. The hybrid genes were constructed by reciprocal exchanges of the amino-terminal and carboxy-terminal parts of the beta -glucanase encoding genes from Bacillus amyloliquefaciens and Bacillus macerans. The resulting thermostable (1,3-1,4)- beta -glucanases retain a significant enzymatic activity at temperatures exceeding 65 DEG C and at pH values below 5.0.
摘要翻译：新型混合热稳定剂（1,3-1,4）-β-葡聚糖酶，它们在食品制造和饲料制造中的应用，编码这种葡聚糖酶的DNA片段，表达DNA片段的生物体和制备热稳定剂的方法（1,3-1 ，4）β-葡聚糖酶。构建了编码芽孢杆菌（1,3-1,4）-β-葡聚糖酶的杂交融合基因，其基因产物比迄今已知的任何（1,3-1,4）-β-葡聚糖酶更耐热。通过互补交换来自解淀粉芽孢杆菌和芽孢杆菌的β-葡聚糖酶编码基因的氨基末端部分和羧基末端部分构建杂交基因。所得到的热稳定性（1,3-1,4）-β-葡聚糖酶在超过65℃的温度和pH值低于5.0时保持显着的酶活性。

2. 发明授权

US5034276A Sizing for glass fibers 失效
标题翻译：玻璃纤维尺寸
公开(公告)号：US5034276A
公开(公告)日：1991-07-23
申请号：US481806
申请日：1990-02-20
申请人： Manfred Zwiersch , Edith Mader , Karl-Heinz Freitag , Rosemarie Plonka , Marianne Radatz , Heinz Momberg , Manfred Barth
发明人： Manfred Zwiersch , Edith Mader , Karl-Heinz Freitag , Rosemarie Plonka , Marianne Radatz , Heinz Momberg , Manfred Barth
IPC分类号： C03C25/10 , C03C25/40 , C07F7/18
CPC分类号： C07F7/1836 , C03C25/40 , Y10T428/2962
摘要： The invention relates to a sizing for the treatment of glass fibers, which are incorporated into thermoplastic materials as reinforcement. The sizing contains a Schiff's base, preferably in concentrations of 0.05 to 2% by weight, based on the dry matter. Through the use of a sizing with this component, the tensile and impact strength properties of the glass fiber-reinforced thermoplastic materials are improved significantly.Key WordsSizing, glass fibers, roving preparation, glass fiber reinforcement, thermoplastic materials, tensile properties, tenacity properties.
摘要翻译：本发明涉及用于处理玻璃纤维的施胶剂，其被加入热塑性材料中作为增强剂。基于干物质，上浆含有希夫碱，优选浓度为0.05-2％（重量）。通过使用该部件的尺寸，玻璃纤维增强热塑性材料的拉伸和冲击强度性能得到显着改善。关键词尺寸，玻璃纤维，粗纱制备，玻璃纤维增强，热塑性材料，拉伸性能，韧性等。

3. 发明授权

US4940325A Device for the investigation of highly resolved partial spectra of an echelle spectrum 失效
标题翻译：用于调查échelle光谱的高度分辨的部分光谱的装置
公开(公告)号：US4940325A
公开(公告)日：1990-07-10
申请号：US320674
申请日：1989-03-08
申请人： Helmut Becker-Ross , Stefan Florek , Reinhard Tischendorf , Klaus-Eberhard Engel , Wolf-Ekkehard Matzke , Susanne Engel
发明人： Helmut Becker-Ross , Stefan Florek , Reinhard Tischendorf , Klaus-Eberhard Engel , Wolf-Ekkehard Matzke , Susanne Engel
IPC分类号： G01J3/18 , G01J3/28
CPC分类号： G01J3/1809 , G01J3/2803
摘要： The invention refers to a device for investigating highly resolved partial spectra of an echelle spectrum, being applicable to the simultaneous determination of the intensities of different spectral elements of a radiation spectrum produced by an echelle spectrometer. The device consists of a position-resolving photoelectric detector including several photosensors arranged on an IC chip, where said photosensors are arranged on the chip surface discretely at the positions of preselected spectral lines, each of the photosensors consisting of a CCD sensor row and a logic circuit which, depending on activation levels, enables supply potentials and clock signals to be connected and output signals to be transferred to a common output signal line; the areas of the individual sensor elements of the CCD sensor rows being matched to the spectral elements of the echelle spectrum and extending successively in the direction of dispersion of the echelle grating; the total number of sensor elements of all CCD sensor rows on the chip being smaller than the number of spectral elements in the echelle spectrum; and a digital logic circuit enabling, by means of the activation levels managed by it, the serial readout of the signals from a selsotable subset of all CCD sensor rows in a selectable order of succession, via the common output signal line, depending on external control signals.
摘要翻译：本发明涉及一种用于研究échelle光谱的高度分辨的部分光谱的装置，其适用于同时确定由échelle光谱仪产生的辐射光谱的不同光谱元素的强度。该装置包括位置分辨光电检测器，其包括布置在IC芯片上的几个光电传感器，其中所述光电传感器分散地布置在芯片表面上的预选光谱线的位置处，每个光电传感器由CCD传感器行和逻辑取决于激活电平使得电源电位和时钟信号被连接并且输出信号被传送到公共输出信号线的电路; CCD传感器行的各个传感器元件的区域与échelle光谱的光谱元件匹配并且沿着échelle光栅的色散方向连续延伸; 芯片上所有CCD传感器行的传感器元件的总数小于échelle光谱中的光谱元素的数量; 以及数字逻辑电路，通过其所管理的激活电平，可以根据外部控制通过公共输出信号线从所有CCD传感器行的可控子集以可选择的顺序读取信号信号。

4. 发明授权

US4882127A Device for solid phase sequencing of nucleic acid fragments 失效
标题翻译：核酸片段固相测序装置
公开(公告)号：US4882127A
公开(公告)日：1989-11-21
申请号：US120092
申请日：1987-11-12
申请人： Andre Rosenthal , Hans-Dieter Hunger , Horst Kagelmaker , Monika Gratschus
发明人： Andre Rosenthal , Hans-Dieter Hunger , Horst Kagelmaker , Monika Gratschus
IPC分类号： B01L7/00 , C12Q1/68 , G01N35/10
CPC分类号： G01N35/10 , B01L7/52 , C12Q1/6869 , G01N2001/288 , Y10T436/143333
摘要： A device for solid phase sequencing of nucleic acid segments comprises a plurality of sequencing blocks for storaging and processing n nucleic acid fragments immobilized on carrier segments or in homogenous phase during a sequencing process, each of a sample dosing device for immobilizing n labeled nucleic acid samples from the sequencing blocks onto areas of a carrier matrix in a simultaneous fashion and including a capillary holder and a plurality of capillaries corresponding to the number n of different nucleic acid samples to be sequences and arranged according to the distribution of the reaction vessels with a distance between capillary orifices from the capillary holder greater than a depth of the reaction vessels, a plurality of carrier holders for fixing carrier matrices in a predetermined position during the immobilization of the nucleic acid samples with the sample dosing device and each having a recess corresponding to respective dimensions of the carrier matrix and a plurality of bores with a number and distribution pattern corresponding to those of the reaction vessels and with a diameter which is somewhat larger than a diameter of the capillary, a punch for simultaneous punching of n immobilized and chemically modified nucleic acid samples as individual carrier segments out of the carrier matrix into the sequencing blocks and including a punching plate and a plurality of punching pins with a number and distribution pattern which also corresponds to those of the reaction vessels.
摘要翻译：用于核酸片段的固相测序的装置包含多个测序块，用于在测序过程期间存储和处理固定在载体片段上或在同质相中的n个核酸片段，用于固定n个标记的核酸样品的样品定量装置从测序块以同时的方式存在于载体基质的区域上，并且包括毛细管保持器和对应于作为序列的不同核酸样品的数目n的多个毛细管并且根据具有一定距离的反应容器的分布在来自毛细管支架的毛细孔之间大于反应容器的深度的多个载体保持器，用于在将核酸样品固定到样品计量装置期间将载体基体固定在预定位置，并且每个具有对应于相应载体矩阵的尺寸和复数具有与反应容器的数量和分布模式对应的直径大于毛细管直径的孔的孔，用于同时冲压n个固定化和化学修饰的核酸样品作为各个载体片段的冲头的载体基质转移到测序块中，并且包括冲孔板和多个也与反应容器对应的数量和分布图形的冲压销。

5. 发明授权

US4849077A Process for solid phase-sequencing of nucleic acid fragments 失效
标题翻译：核酸片段的固相序列测定方法
公开(公告)号：US4849077A
公开(公告)日：1989-07-18
申请号：US761107
申请日：1985-07-31
申请人： Andre Rosenthal , Hans-Dieter Hunger , Horst Kagelmaker , Monika Graatschus
发明人： Andre Rosenthal , Hans-Dieter Hunger , Horst Kagelmaker , Monika Graatschus
IPC分类号： B01L7/00 , C12Q1/68 , G01N35/10
CPC分类号： G01N35/10 , B01L7/52 , C12Q1/6869 , G01N2001/288 , Y10T436/143333
摘要： A process for solid phase sequencing of nucleic acid fragments is disclosed. The object of the invention is to provide a sequencing process which enables the simultaneous sequencing of large amounts of long and short nucleic acid fragments and which is optionally automated. Thus, a solid support combining mechanical stability, anion exchange characteristics, and chemical elution of nucleic acids off the support is used. Immobilized nucleic acid fragments are chemically modified and subsequently the nucleic acid backbone is cleaved and simultaneously or subsequently said fragments are eluted by chemical means. The present invention is applied to molecular biology and gene technology.
摘要翻译：公开了一种用于核酸片段的固相测序的方法。本发明的目的是提供一种测序方法，其能够对大量长和短核酸片段进行同时测序，并且其任选地是自动化的。因此，使用结合机械稳定性，阴离子交换特性和离开载体的核酸的化学洗脱的固体支持物。固定的核酸片段被化学修饰，随后核酸骨架被切割并同时或随后所述片段通过化学方法被洗脱。本发明应用于分子生物学和基因技术。

6. 发明授权

US4716103A Chemically active triazine support composition 失效
标题翻译：化学活性三嗪支持组合物
公开(公告)号：US4716103A
公开(公告)日：1987-12-29
申请号：US638882
申请日：1984-08-08
申请人： Hans-Dieter Hunger , Charles Coutelle
发明人： Hans-Dieter Hunger , Charles Coutelle
IPC分类号： C08K5/3492 , C08L101/00 , C12N11/02 , C12N11/12 , C12Q1/70 , G01N33/543 , G01N33/544 , C12Q1/68 , C07D251/00
CPC分类号： G01N33/544 , C08K5/3492 , C08L101/00 , C12N11/02 , C12N11/12 , C12Q1/70 , G01N33/543 , Y10S530/814
摘要： A support composition based on triazine derivatives is prepared having high binding capacity. The support composition contains a polymer compound having no triazine groups, a polymer compound with 4,6-dihalogen-1,3,5-triazine groups, a 2,4,6-trihalogen-1,3,5-triazine filler component and an alkali metal halogenide filler component. The support is useful in molecular biology particularly for the fixing of biomacromolecules and for clinical and analytical detection of immunologically significant factors or organic or inorganic components of liquids.
摘要翻译：制备具有高结合能力的基于三嗪衍生物的载体组合物。载体组合物含有不含三嗪基团的高分子化合物，具有4,6-二卤代-1,3,5-三嗪基团的高分子化合物，2,4,6-三卤素-1,3,5-三嗪填料组分和碱金属卤化物填料组分。该支持物可用于分子生物学，特别是用于固定生物大分子以及临床和分析检测免疫学重要因素或液体的有机或无机成分。

7. 发明授权

US4714768A N-(chlorocarbonyloxy)-5-norbornene-2,3-dicarboximide, process for its production and its use 失效
标题翻译： N-（氯羰基氧基）-5-降冰片烯-2,3-二甲酰亚胺，其生产及其用途
公开(公告)号：US4714768A
公开(公告)日：1987-12-22
申请号：US644777
申请日：1984-08-27
申请人： Peter Henklein , Manfred Becker , Werner Buttner , Fritz Loth , Horts Dautzenberg , Klaus Forner , Rudolf Dolling , Karl-Heinz Graul , Wolf-Rainer Halatsch , Christian Rupprich
发明人： Peter Henklein , Manfred Becker , Werner Buttner , Fritz Loth , Horts Dautzenberg , Klaus Forner , Rudolf Dolling , Karl-Heinz Graul , Wolf-Rainer Halatsch , Christian Rupprich
IPC分类号： C07D209/76 , C07K1/04 , C07K1/113 , C07K1/22 , C07K14/575 , C07K16/00 , C07K17/06 , C08B3/00 , C08F8/30 , C08F8/34 , C12N11/06 , G01N33/543 , G01N33/544 , C07D209/02
CPC分类号： C07D209/76 , C12N11/06 , G01N33/54353 , G01N33/544
摘要： A new compound is disclosed, N-(chlorocarbonyloxy-5-norbornene-2,3-dicarboximide I, in addition to processes for its production and employment of the compound as starting product for the manufacture of non-symmetrical carbonate, urethane-protected amino acid or peptide, activated carrier and carrier-ligand-complexes. The new compound is reacted with alcohols as well as with hydroxyl-group-containing or amino-group-containing polymers.
摘要翻译：公开了一种新化合物，除了其生产和使用化合物作为制备非对称碳酸酯，氨基甲酸酯保护的氨基的起始产物的方法之外，N-（氯羰氧基-5-降冰片烯-2,3-二甲酰亚胺I）酸或肽，活化的载体和载体 - 配体 - 络合物，新化合物与醇以及含羟基或含氨基的聚合物反应。

8. 发明授权

US4410709A Sulfobetaines 失效
标题翻译：磺胺甜菜碱
公开(公告)号：US4410709A
公开(公告)日：1983-10-18
申请号：US281432
申请日：1981-07-08
申请人： Roland Ohme , Detlef Ballschuh , Jochen Rusche , Horst Seibt , Kristina Geneis , Gunter Kretzschmar
发明人： Roland Ohme , Detlef Ballschuh , Jochen Rusche , Horst Seibt , Kristina Geneis , Gunter Kretzschmar
IPC分类号： C09K3/16 , B01F17/18 , B01J23/00 , B01J23/22 , B01J23/34 , B01J23/70 , B01J27/00 , B01J27/02 , C07B61/00 , C07D207/08 , C11D1/92
CPC分类号： C07D207/08 , B01F17/0035 , B01F17/0042 , B01F17/0057 , C11D1/92
摘要： The invention relates to new sulfobetaines and a process for the manufacture of cyclic sulfobetaines of a substituted 4-sulfomethyl-pyrrolidinium betaine type.Diallylammonium combinations are converted with salts of sulfurous acid, under good mixing and mild reaction conditions, in the presence of catalytic acting transition metallic ions of the first, fifth, seventh or eighth secondary groups of the Period Table and initiators at pH 2-9. The process is technologically easily carried out, requires only little energy, while the combinations according to this invention can be selectively manufactured with a nearly quantitative yield. The fact that no carcinogenic alkylates are employed, technical chemicals and tap water may be used, and hardly any by-products are formed, may be regarded as further advantages of this process.The substances can be used for multiple purposes, for instance as conductive coating and antistatic materials. Moreover, long-chained substituted compounds have valuable surface tension qualities and an excellent cleaning power at low temperatures.
摘要翻译：本发明涉及新的磺基甜菜碱和一种制备取代的4-磺基甲基 - 吡咯烷鎓甜菜碱型环状磺基甜菜碱的方法。二烯丙基铵组合在良好的混合和温和的反应条件下，在周期表的第一，第五，第七或第八次级基团的催化作用的过渡金属离子和pH 2-9的引发剂的存在下，用亚硫酸的盐转化。该方法在技术上容易地进行，仅需要很少的能量，而根据本发明的组合可以以几乎定量的产率选择性地制造。可以使用不使用致癌烷基化物的事实，技术化学品和自来水，并且几乎不产生任何副产物，这可被认为是该方法的进一步优点。该物质可用于多种用途，例如导电涂料和抗静电材料。此外，长链取代的化合物在低温下具有有价值的表面张力特性和优异的清洁力。

9. 发明授权

US5091494A Copolyester amides, and method for making 失效
标题翻译：共聚酰胺和制备方法
公开(公告)号：US5091494A
公开(公告)日：1992-02-25
申请号：US559855
申请日：1990-07-30
申请人： Dirk Leistner , Frank Bohme , Doris Pospiech , Manfred Ratzsch , Christian Vieth , Michael Stephan
发明人： Dirk Leistner , Frank Bohme , Doris Pospiech , Manfred Ratzsch , Christian Vieth , Michael Stephan
IPC分类号： C08G69/44
CPC分类号： C08G69/44
摘要： The invention relates to new aromatic/aliphatic and/or alicyclic copolyester amides with high molecular weights, without crosslinking. A subsequent solid phase condensation is avoided or is greatly limited in time. The occurrence of thermal decomposition reactions, dark discolorations of the polymers as well as the formation of block structures are avoided.
摘要翻译：本发明涉及具有高分子量，没有交联的新的芳族/脂族和/或脂环族共聚酯酰胺。随后的固相冷凝被避免或在时间上受到很大的限制。避免了热分解反应的发生，聚合物的暗变色以及嵌段结构的形成。

10. 发明授权

US4728562A Dielectric composition 失效
标题翻译：介电组成
公开(公告)号：US4728562A
公开(公告)日：1988-03-01
申请号：US738022
申请日：1985-05-24
申请人： Hans-Jurgen Gesemann , Mary Krawietz , Winfried Schaffrath
发明人： Hans-Jurgen Gesemann , Mary Krawietz , Winfried Schaffrath
IPC分类号： C01G33/00 , H01B3/12 , H01G4/12 , C04B33/26
CPC分类号： H01B3/12 , Y10S428/901 , Y10T428/24917 , Y10T428/24926
摘要： The invention relates to a dielectric composition which, in particular, is useable in the thick layer technology for making printed condensers by means of the screen printing process. The composition contains as a dielectric active substance Bi.sub.2 [A.sub.2-x.sup.a+ Nb.sub.x ], whereby the condition a (2-x)+5x=8 is applied and A are one of a plurality of metal ions from the group Mg.sup.2+,Ni.sup.2+,Zn.sup.2+,Co.sup.2+,Ca.sup.2+,Sn.sup.2+,Mn.sup.2+,Co.sup.3+ Mn.sup.3+,Al.sup.3+ and Cr.sup.3+.
摘要翻译：本发明涉及一种电介质组合物，其特别可用于通过丝网印刷工艺制造印刷电容器的厚层技术。该组合物含有介电活性物质Bi2 [A2-xa + Nbx]，由此施加条件a（2-x）+ 5x = 8，A是Mg2 +，Ni2 +，Zn2 + ，Co2 +，Ca2 +，Sn2 +，Mn2 +，Co3 + Mn3 +，Al3 +和Cr3 +。

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