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    • 81. 发明公开
    • 폴리뉴클레오티드를 이용한 제품 인증 장치 및 방법
    • 用于使用多核苷酸验证产品的装置和方法
    • KR1020110078185A
    • 2011-07-07
    • KR1020090134927
    • 2009-12-30
    • 삼성전자주식회사
    • 이주원박경희
    • C12Q1/68C12N15/11G06K7/00
    • C12Q1/6816B01L3/502707C12M3/00C12Q1/68C12Q1/6806C12Q1/6813C12Q1/6844C12Q2531/125C12Q2533/107C12Q2563/173
    • PURPOSE: An apparatus and method for product verification using a polynucleotide is provided to easily distinguish genuine articles and counterfeit articles. CONSTITUTION: A method for product verification comprises: a step of hybridizing a probe polynucleotide and a target polynucleotide; a step of linking the ends of a first segment and a second segment on the probe polynucleotide; a step of amplifying the probe polynucleotide; and a step of detecting the amplified product. The amplification is performed by rolling circle amplification or multiple displacement amplification. An apparatus for product verification comprises: a sample injection part(10) containing the prove polynucleotide and ligase; an amplification part(20) containing polynucleotide polymerase, primer, and dNTP; and a detection part for detecting the amplified polynucleotide.
    • 目的:提供一种使用多核苷酸进行产品验证的设备和方法,可轻松区分真品和假冒物品。 构成:产品验证的方法包括:将探针多核苷酸和靶多核苷酸杂交的步骤; 将探针多核苷酸上的第一片段和第二片段的末端连接的步骤; 扩增探针多核苷酸的步骤; 以及检测扩增产物的步骤。 通过滚环放大或多位置扩增进行扩增。 一种用于产品验证的装置,包括:含有证明多核苷酸和连接酶的样品注射部分(10) 包含多聚核苷酸聚合酶,引物和dNTP的扩增部分(20); 和用于检测扩增的多核苷酸的检测部分。
    • 82. 发明公开
    • 비드-기초 서열화를 위한 시약, 방법, 및 라이브러리
    • 用于基于BEAD的测序的试剂,方法和图谱
    • KR1020070112785A
    • 2007-11-27
    • KR1020077019819
    • 2006-02-01
    • 에이젠코트 바이오사이언스 코오포레이션
    • 맥커난,케빈블란차드,알란코틀러,레브코스타,지나
    • C12Q1/68G06F19/28
    • C12Q1/6874B82Y15/00B82Y30/00C12Q1/68C12Q1/6837C12Q1/6844C12Q1/6869C12Q2533/107C12Q2537/165C12Q2565/102C12Q2565/137C12Q2565/513C12Q2565/518C12Q2565/537G06F19/28
    • The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. In certain embodiments the methods make use of extension probes containing an abasic residue or a damaged base and employ agents appropriate to cleave linkages between a nucleoside and an abasic residue and/or agents appropriate to remove a damaged base from a nucleic acid. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to beads, which are immobilized in or on a semi-solid support. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages or trigger residues that are suitable for use in the method. In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides. The invention further provides efficient methods for preparing templates, particularly for performing sequencing multiple different templates in parallel. The invention also provides methods for performing ligation and cleavage. The invention also provides new libraries of nucleic acid fragments containing paired tags, and methods of preparing microparticles having multiple different templates (e.g., containing paired tags) attached thereto and of sequencing the templates individually. The invention also provides automated sequencing systems, flow cells, image processing methods, and computer-readable media that store computer-executable instructions (e.g., to perform the image-processing methods) and/or sequence information. In certain embodiments the sequence information is stored in a database.
    • 本发明提供了通过沿着单链模板进行连续循环的双链延伸来确定核酸序列的方法。 循环包括延伸,连接和优选切割的步骤。 在某些实施方案中,所述方法利用含有硫代磷酸酯键的延伸探针,并使用适合切割这种连接的试剂。 在某些实施方案中,所述方法利用含有无碱基残基或受损碱基的延伸探针,并使用适于切割核苷和脱碱基残基之间的连接的试剂和/或适于从核酸中除去受损碱基的试剂。 本发明提供使用至少两个可区分标记的探针家族确定关于序列的信息的方法。 在某些实施方案中,该方法在每个周期中从模板中的多个核苷酸中的每一个获取少于2位的信息。 在某些实施方案中,测序反应在附着于珠上的模板上进行,所述模板固定在半固体支持物中或半固体支持物上。 本发明进一步提供了包含适合用于该方法的硫代磷酸酯键或触发性残基的标记的延伸探针的集合。 此外,本发明包括通过除去初始化寡核苷酸和延伸的链并使用不同的初始化寡核苷酸进行后续反应,在单个模板上进行多个测序反应。 本发明还提供了用于制备模板的有效方法,特别是用于并行地进行多个不同模板的排序。 本发明还提供了进行连接和切割的方法。 本发明还提供了含有配对标签的核酸片段的新文库,以及制备具有多个不同模板(例如,含有配对标签)的微粒和分别对模板进行测序的方法。 本发明还提供自动排序系统,流动池,图像处理方法和存储计算机可执行指令(例如,执行图像处理方法)和/或序列信息的计算机可读介质。 在某些实施例中,序列信息存储在数据库中。
    • 88. 发明专利
    • 同時偵測多種微小核酸之方法
    • 同时侦测多种微小核酸之方法
    • TW201122473A
    • 2011-07-01
    • TW098145130
    • 2009-12-25
    • 長庚大學
    • 張柏齡張玉生陳淑貞陳華鍵
    • G01N
    • C12Q1/6816C12Q2565/137C12Q2565/102C12Q2537/143C12Q2537/162C12Q2533/107
    • 本發明提供一種同時偵測多種微小核酸之方法,其係先將待測的樣本試劑與螢光探針以及不同長度的橋樑核酸混合而成受測液,過程中先使其探針與待測核酸與橋樑核酸雜交,隨後加入一接合酶,使試劑內的短核酸分子與螢光探針以橋樑核酸為模版進行接合反應。接著再導入受測液於毛細管中,並施加電場使雜交後的產物進行電泳分離,最後以雷射激發這些產物,並分析產物所釋放之螢光訊號,故可同時於一根毛細管內同時偵測出待測的樣本內之多種短核酸分子。
    • 本发明提供一种同时侦测多种微小核酸之方法,其系先将待测的样本试剂与萤光探针以及不同长度的桥梁核酸混合而成受测液,过程中先使其探针与待测核酸与桥梁核酸杂交,随后加入一接合酶,使试剂内的短核酸分子与萤光探针以桥梁核酸为模版进行接合反应。接着再导入受测液于毛细管中,并施加电场使杂交后的产物进行电泳分离,最后以激光激发这些产物,并分析产物所释放之萤光信号,故可同时于一根毛细管内同时侦测出待测的样本内之多种短核酸分子。