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    • 87. 发明申请
    • Nucleotide sequences coding for the pknD gene
    • 编码pknD基因的核苷酸序列
    • US20050255566A1
    • 2005-11-17
    • US11110837
    • 2005-04-21
    • Brigitte BatheIndra SchroederMike FarwickThomas Hermann
    • Brigitte BatheIndra SchroederMike FarwickThomas Hermann
    • C12N9/12C12Q1/68C07H21/04C12N1/21C12N15/74C12P13/04
    • C12N9/1205C12R1/15
    • An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknD gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
    • 一种分离的多核苷酸,其含有选自以下的多核苷酸序列:(a)与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70%的多核苷酸,(b) 编码含有与SEQ ID No.2的氨基酸序列至少70%相同的氨基酸序列的多肽,(c)与(a)或(b)的多核苷酸互补的多核苷酸和( d)包含(a),(b)或(c)的多核苷酸序列的至少15个连续核苷酸的多核苷酸,以及使用棒状杆菌制备L-氨基酸的发酵方法,其中至少pknD基因是 扩增和使用含有本发明序列的多核苷酸作为杂交探针。