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81. 发明专利

CA2325350A1 MICROBIAL STRAINS AND PROCESSES FOR THE MANUFACTURE OF BIOMATERIALS 未知
公开(公告)号：CA2325350A1
公开(公告)日：1999-10-07
申请号：CA2325350
申请日：1999-03-30
申请人： METABOLIX INC
发明人： BOYNTON LAURA , GERNGROSS TILLMAN U , PEOPLES OLIVER P , HOROWITZ DANIEL M , HUISMAN GJALT W
IPC分类号： C12N15/09 , C12N1/08 , C12N1/21 , C12N9/22 , C12P7/62 , C12P19/04 , C12P19/00
摘要： DNA constructs and genetically engineered microbial strains constructed usin g these DNA constructs, which produce a nuclease enzyme with specificity for D NA and/or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and/or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.

82. 发明专利

BR112013027641A2 processo verde para produzir poli-hidroxialcanoatos e produtos químicos com o uso de uma matéria-prima renovável 未知
公开(公告)号：BR112013027641A2
公开(公告)日：2016-11-29
申请号：BR112013027641
申请日：2012-04-26
申请人： METABOLIX INC
发明人： MCCHALICHER CHRISTOPHER W J , WALSEM JOHAN VAN , PEOPLES OLIVER P , ENO RICHARD P , RAMSEIER THOMAS M , ZHANG ZHIGANG
IPC分类号： C12N1/20 , C12N15/52
摘要： resumo patente de invenção: "processo verde para produzir poli-hidroxialcanoatos e produtos químicos com o uso de uma matéria-prima renovável". a presente invenção refere-se a organismos geneticamente modificados para produzir produtos úteis quando cultivados em etanol como uma fonte de carbono. os organismos são geneticamente modificados para produzir vários produtos úteis, como poli-hidroxialcanoatos, dióis, diácidos, álcoois superiores e outros produtos químicos.

83. 发明专利

AU2010221132B2 Stable, fertile, high polyhydroxyalkanoate producing plants and methods of producing them 未知
公开(公告)号：AU2010221132B2
公开(公告)日：2013-11-21
申请号：AU2010221132
申请日：2010-03-05
申请人： METABOLIX INC
发明人： BOHMERT-TATAREV KAREN , MCAVOY SUSAN , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： C12N15/82
摘要： Transgenic plants that produce high levels of polyhydroxybutyrate and methods of producing them are provided. In a preferred embodiment the transgenic plants are produced using plastid transformation technologies and utilize genes which are codon optimized. Stably transformed plants able to produce greater than 10% dwt PHB in tissues are also provided.

84. 发明专利

AT521710T 未知
公开(公告)号：AT521710T
公开(公告)日：2011-09-15
申请号：AT06738572
申请日：2006-03-16
申请人： METABOLIX INC
发明人： KOURTZ LAURALYNN , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： C12N15/82 , A01H5/00 , C12P7/62
摘要： Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent.

85. 发明专利

CA2773707A1 GENERATION OF HIGH POLYHYDROXYBUTRATE PRODUCING OILSEEDS WITH IMPROVED GERMINATION AND SEEDLING ESTABLISHMENT 未知
公开(公告)号：CA2773707A1
公开(公告)日：2011-03-24
申请号：CA2773707
申请日：2010-09-15
申请人： METABOLIX INC
发明人： PATTERSON NII , TANG JIHONG , HAN JIXIANG , TAVVA VENKATA , HERTIG ANDREW , ZHANG ZHIGANG , RAMSEIER THOMAS MARTIN , BOHMERT-TATAREV KAREN , PEOPLES OLIVER P , SNELL KRISTI D
IPC分类号： C12N15/82 , A01H5/00 , C12P7/62
摘要： Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates ("PHA") are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate ("PHB"). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.

86. 发明专利

CA2303070C BIOLOGICAL SYSTEMS FOR MANUFACTURE OF POLYHYDROXYLALKANOATE POLYMERS CONTAINING 4-HYDROXYACIDS 未知
公开(公告)号：CA2303070C
公开(公告)日：2011-03-15
申请号：CA2303070
申请日：1998-09-18
申请人： METABOLIX INC
发明人： HUISMAN GJALT W , SKRALY FRANK A , MARTIN DAVID P , PEOPLES OLIVER P
IPC分类号： C12N15/09 , C12N15/54 , C08G63/06 , C12N1/21 , C12N9/02 , C12N9/10 , C12N15/53 , C12N15/70 , C12N15/82 , C12P7/52 , C12P7/62 , C12R1/19
摘要： The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtained by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, .alpha.-ketoglutarate, or succinate as substrate.

87. 发明专利

DE69938105D1 未知
公开(公告)号：DE69938105D1
公开(公告)日：2008-03-20
申请号：DE69938105
申请日：1999-08-04
申请人： METABOLIX INC
发明人： SKRALY FRANK , PEOPLES OLIVER P
IPC分类号： A01H1/00 , C12P7/42 , C08G8/04 , C08G8/26 , C12N1/21 , C12N15/00 , C12N15/09 , C12N15/52 , C12N15/82 , C12P7/62 , C12R1/19
摘要： Organisms arc provided which express enzymes such as glycerol dehydratase, diol dehydratase, acyl-CoA transferase, acyl-CoA synthetase ²-ketothiolase, a;xtoaeetyl-CoA reductase, PHA synthase, glycerol-3-phosphate dehydrogenase and glycerol-3-pbospharase, which are useful for the production of PHAs. In some cases one or more of these genes are native to the host organism and the remainder are provided from transgenes. These organisms produce poJy(3-hydroxyalkanoare) homopolymers or co-polymers incorporating 3-hydroxypropionate or 3-hydroxyvalerate monomers wherein the 3-hydroxynropionate and 3-hydroxyvalerate units are derived from the enzyme catalysed conversion of diols. Suitable diols that can be used include 1,2-propanediol, 1,3-propanediol and glycerol. Biochemical pathways for obtaining the glycerol from normal cellular metabolites are also described. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates including 1,3-propanediol, 3-hydroxypropionaldehyde, -acrylics, malonic acid, esters and amines.

88. 发明专利

CA2234965C METHODS FOR ISOLATING POLYHYDROXYALKANOATES FROM PLANTS 未知
公开(公告)号：CA2234965C
公开(公告)日：2007-08-14
申请号：CA2234965
申请日：1996-10-23
申请人： METABOLIX INC
发明人： WILLIAMS SIMON F , PEOPLES OLIVER P , MARTIN DAVID P
IPC分类号： C08G63/06 , C08G63/89 , C12P7/62
摘要： Methods are provided for separating polyhydroxyalkanoates ("PHAs") from plants, such as transgenic oil crop plants. The methods advantageously permit both the oil and the PHAs to be recovered from the pla nt biomass. To isolate the PHAs, in one embodiment, a biomass derived from an oil crop plant is pre-processed, for example by grinding, crushing or rolling. The oil then is extracted from the biomass with a first solvent in which the oil is soluble and in which the PH As are not highly soluble to remove the oil. The biomass then can be extracted with a second solvent in which the PHA is soluble, to separate the PHA from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent, such as an enzyme, to chemically transform the PHA into a PHA derivative. The PHA, derivative then is separated from the mixture using, for example, a physical separation process such as distillation, extraction or chromatography. Advantageously, using the method, the plant oils, the PHAs a nd PHA derivatives can be recovered and purified on a large scale from oil containing plants such as transgenic oil crop plants.

89. 发明专利

CA2600882A1 CHEMICALLY INDUCIBLE EXPRESSION OF BIOSYNTHETIC PATHWAYS 未知
公开(公告)号：CA2600882A1
公开(公告)日：2006-09-28
申请号：CA2600882
申请日：2006-03-16
申请人： METABOLIX INC
发明人： KOURTZ LAURALYNN , SNELL KRISTI D , PEOPLES OLIVER P
IPC分类号： C12N15/82 , A01H5/00 , C12P7/62
摘要： Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent.

90. 发明专利

AT283056T 未知
公开(公告)号：AT283056T
公开(公告)日：2004-12-15
申请号：AT99935846
申请日：1999-07-22
申请人： METABOLIX INC
发明人： MARTIN DAVID P , PEOPLES OLIVER P , WILLIAMS SIMON F , ZHONG LUHUA
IPC分类号： A23K1/16 , A23K1/18 , A23L1/30 , A61K31/00 , A61K31/19 , A61K31/765 , A61P3/10 , A61P7/00 , A61P21/04 , A61P25/00 , A61P25/08 , A61P25/28 , A61P43/00 , A61P3/00

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