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    • 86. 发明授权
    • Modified Cry3A toxins and nucleic acid sequences coding therefor
    • 修饰的Cry3A毒素及其编码的核酸序列
    • US07569363B2
    • 2009-08-04
    • US11834037
    • 2007-08-06
    • Eric ChenCheryl Stacy
    • Eric ChenCheryl Stacy
    • C12P21/06C07H21/04
    • C12N15/8286C07K14/325Y02A40/162
    • Methods for making a modified Cry3A toxin are disclosed. Such methods include the insertion of a protease recognition site that is recognized by a gut protease of a target insect, such as corn rootworm, into at least one position of a Cry3A toxin so that a modified Cry3A toxin is thus designed. The coding sequence of the modified Cry3A toxin may be transformed into a host cell and the host cell grown under conditions that allow the host cell to produce the modified Cry3A toxin. The host cell may be a plant cell and the plant may be comprised in a transgenic plant. Thus, the transgenic plant may be used to produce the modified Cry3A toxin.
    • 公开了制备修饰的Cry3A毒素的方法。 这样的方法包括将目标昆虫(例如玉米根虫)的肠蛋白酶识别的蛋白酶识别位点插入Cry3A毒素的至少一个位置,从而设计出修饰的Cry3A毒素。 修饰的Cry3A毒素的编码序列可以转化到宿主细胞中,宿主细胞在允许宿主细胞产生修饰的Cry3A毒素的条件下生长。 宿主细胞可以是植物细胞,并且植物可以包含在转基因植物中。 因此,转基因植物可用于产生经修饰的Cry3A毒素。
    • 90. 发明授权
    • Modified Cry3A toxins
    • 改良的Cry3A毒素
    • US07276583B2
    • 2007-10-02
    • US11294220
    • 2005-12-05
    • Eric ChenCheryl Stacy
    • Eric ChenCheryl Stacy
    • C07K14/00
    • C12N15/8286C07K14/325Y02A40/162
    • Compositions and methods for controlling plant pests are disclosed. In particular, novel nucleic acid sequences encoding modified Cry3A toxins having increased toxicity to corn rootworm are provided. By inserting a protease recognition site, such as cathepsin G, that is recognized by a gut protease of a target insect in at least one position of a Cry3A toxin a modified Cry3A toxin having significantly greater toxicity, particularly to western and northern corn rootworm is designed. Further, a method of making the modified Cry3A toxins and methods of using the modified cry3A nucleic acid sequences, for example in microorganisms to control insects or in transgenic plants to confer protection from insect damage, and a method of using the modified Cry3A toxins, and compositions and formulations comprising the modified Cry3A toxins, for example applying the modified Cry3A toxins or compositions or formulations to insect-infested areas, or to prophylactically treat insect-susceptible areas or plants to confer protection against the insect pests are disclosed.
    • 公开了用于控制植物害虫的组合物和方法。 特别地,提供了对玉米根虫具有增加的毒性的编码修饰的Cry3A毒素的新型核酸序列。 通过在蛋白酶识别位点(例如组织蛋白酶G)中插入由至少一种Cry3A毒素的至少一种位置的目标昆虫的肠蛋白酶识别出具有显着更大毒性的改良Cry3A毒素,特别是西方和北方玉米根虫的蛋白酶识别位点 。 此外,制备修饰的Cry3A毒素的方法和使用经修饰的cry3A核酸序列的方法,例如在微生物中控制昆虫或在转基因植物中赋予防御昆虫损伤的方法,以及使用经修饰的Cry3A毒素的方法,以及 公开了包含修饰的Cry3A毒素的组合物和制剂,例如将经修饰的Cry3A毒素或组合物或制剂施用于昆虫侵袭区域,或用于预防性治疗昆虫敏感区域或植物以赋予对昆虫害虫的保护。