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81. 发明专利

CO2019014476A2 Detector con ruido de intervalo de fluorescencia reducido 未知
公开(公告)号：CO2019014476A2
公开(公告)日：2020-04-13
申请号：CO2019014476
申请日：2019-12-20
申请人： ILLUMINA INC
发明人： PINTO JOSEPH , FUNG TRACY H , SABOUNCHI POORYA , HIRSCHBEIN BERNARD , KHURANA TARUN , SMITH RANDALL , FENG WENYI
IPC分类号： G01N21/64
摘要： En este documento se expone un dispositivo que comprende una estructura que define una superficie de detector configurada para soportar sustancias biológicas o químicas, y una matriz de sensores que comprende sensores de luz y circuitos para transmitir señales de datos usando los fotones detectados por los sensores de luz.

82. 发明专利

CR20190584A DETECTOR CON RUIDO DE INTERVALO DE FLUORESCENCIA REDUCIDO 未知
公开(公告)号：CR20190584A
公开(公告)日：2020-03-06
申请号：CR20190584
申请日：2018-11-30
申请人： ILLUMINA INC
发明人： FUNG TRACY H , HIRSCHBEIN BERNARD , KHURANA TARUN , SMITH RANDALL , FENG WENYI , SABOUNCHI POORYA , PINTO JOSEPH
IPC分类号： G01N21/64
摘要： En este documento se expone un dispositivo que comprende una estructura que define una superficie de detector configurada para soportar sustancias biológicas o químicas, y una matriz de sensores que comprende sensores de luz y circuitos para transmitir señales de datos usando los fotones detectados por los sensores de luz. El dispositivo puede incluir una o más funciones para reducir el ruido del intervalo de fluorescencia en una banda de detección de la matriz de sensores

83. 发明专利

CA3067426A1 FLOW CELL WITH INTEGRATED MANIFOLD 未知
公开(公告)号：CA3067426A1
公开(公告)日：2019-11-07
申请号：CA3067426
申请日：2019-04-30
申请人： ILLUMINA INC
发明人： LIN SZ-CHIN , TAYLOR JAY , RHEE MINSOUNG , FOLEY JENNIFER , COX-MURANAMI WESLEY , DELATTRE CYRIL , KHURANA TARUN , CRIVELLI PAUL
IPC分类号： G01N35/08 , G01N37/00
摘要： In one example, a flow cell includes a plurality of inlet ports sized to receive a flow of reagent from one of a plurality of reagents into the flow cell. An outlet port of the flow cell is sized to pass each flow of reagent out of the flow cell. A flow channel of the flow cell is positioned between, and in fluid communication with, each inlet port and the outlet port. The flow channel includes a manifold section and a detection section. The manifold section has a plurality of manifold branches in fluid communication with a common line, wherein each branch is connected to one of each inlet port. The detection section is in fluid communication with the common line and the outlet port. The detection section is operable to perform a plurality of different chemical reactions between the plurality of reagents and analytes positioned in the detection section.

84. 发明专利

SG11201908680YA METHODS AND COMPOSITIONS FOR PREPARING NUCLEIC ACID LIBRARIES 未知
公开(公告)号：SG11201908680YA
公开(公告)日：2019-10-30
申请号：SG11201908680Y
申请日：2018-03-16
申请人： ILLUMINA INC
发明人： CHEN XI-JUN , KHURANA TARUN
IPC分类号： C12N15/10
摘要： OHbp • - •1 3' 5' 5 P5 adaptor Phosphodiester linkage (S)— \\'‘` `'-J\"? zz/z//t/A OH 3 P7' 5' IP 3) 5'Phosphorylation PT Phosphodiester 64bp linkage Phosphodiester linkage 60bp 5' 4) CircLigase Ligation 5' OH ssDNA Insert 5' 3' OH: OH OH 3' G 5 P 1) 5'Dephosphonflation dsDNA Insert & Dehybridization 64bp 5' P ffeloW mw 3' 5' PT adaptor 2) CircLigase Ligation T4 Polynucleotide Phosphodiester Kinase linkage PT P5 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 27 September 2018 (27.09.2018) WIP0 I PCT 011101 V III o Ho VII IE (10) International Publication Number WO 2018/175258 Al (51) International Patent Classification: Cl 2N 15/10 (2006.01) (21) International Application Number: PCT/US2018/022978 (22) International Filing Date: 16 March 2018 (16.03.2018) English English (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/473,595 20 March 2017 (20.03.2017) US (84) (71) Applicant: ILLUMINA, INC. [US/US]; 5200 Illumina Way, San Diego, CA 92122 (US). (72) Inventors: CHEN, Xi-Jun; C/o Illumina, Inc., 5200 Il- lumina Way, San Diego, CA 92122 (US). KHURANA, Tarun; C/o Illumina, Inc., 5200 Illumina Way, San Diego, CA 92122 (US). (74) Agent: FULLER, Michael, L.; Knobbe Martens Olson & Bear LLP, 2040 Main Street, Fourteenth Floor, Irvine, CA 92614 (US). (54) Title: METHODS AND COMPOSITIONS FOR PREPARING NUCLELIC ACID LIBRARIES GC 5'-P5-ssDNA insert-P7 3' N FIG. 1 1-1 © (57) : Systems, methods and compositions provided herein relate to the preparation of nucleic acid libraries. Some embodi- O ments include the preparation of nucleic acid libraries by ligation of single-stranded nucleic acids. [Continued on next page] WO 2018/175258 Al MIDEDIM011111 1111E111 IIE3010M11111111101111111111111111111 Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))

85. 发明专利

AU2018239186A1 Methods and compositions for preparing nuclelic acid libraries 未知
公开(公告)号：AU2018239186A1
公开(公告)日：2019-10-17
申请号：AU2018239186
申请日：2018-03-16
申请人： ILLUMINA INC
发明人： CHEN XI-JUN , KHURANA TARUN
IPC分类号： C12N15/10
摘要： Systems, methods and compositions provided herein relate to the preparation of nucleic acid libraries. Some embodiments include the preparation of nucleic acid libraries by ligation of single-stranded nucleic acids.

86. 发明专利

AU2017345562A1 Methods for chemical ligation of nucleic acids 未知
公开(公告)号：AU2017345562A1
公开(公告)日：2019-04-18
申请号：AU2017345562
申请日：2017-10-19
申请人： ILLUMINA SINGAPORE PTE LTD , ILLUMINA INC
发明人： TEO YIN NAH , CHEN XI-JUN , KHURANA TARUN
IPC分类号： C12Q1/6806
摘要： Provided herein are methods for preparing nucleic acid libraries, capturing DNA obtained from a limited number of cells, and selectively cleaving ssDNA or dsDNA using various chemical ligation and click chemistry reactions described herein.

87. 发明专利

SG11201809376WA SELF ASSEMBLED PATTERNING USING PATTERNED HYDROPHOBIC SURFACES 未知
公开(公告)号：SG11201809376WA
公开(公告)日：2018-12-28
申请号：SG11201809376W
申请日：2017-05-17
申请人： ILLUMINA INC
发明人： WU YIR-SHYUAN , LIN YAN-YOU , BOWEN M , DELATTRE CYRIL , ABEILLE FABIEN , KHURANA TARUN , RIVAL ARNAUD , SABOUNCHI POORYA , YUAN DAJUN , ROGERT BACIGALUPO MARIA
IPC分类号： G03F7/40 , B82Y30/00 , C12Q1/68 , G01N33/52
摘要： Embodiments provided herewith are directed to self-assembled methods of preparing a patterned surface for sequencing applications including, for example, a patterned flow cell or a patterned surface for digital fluidic devices. The methods utilize photolithography to create a patterned surface with a plurality of microscale or nanoscale contours, separated by hydrophobic interstitial regions, without the need of oxygen plasma treatment during the photolithography process. In addition, the methods avoid the use of any chemical or mechanical polishing steps after the deposition of a gel material to the contours.

88. 发明专利

AU2017267653A1 Self assembled patterning using patterned Hydrophobic surfaces 未知
公开(公告)号：AU2017267653A1
公开(公告)日：2018-11-22
申请号：AU2017267653
申请日：2017-05-17
申请人： ILLUMINA INC
发明人： WU YIR-SHYUAN , LIN YAN-YOU , BOWEN M SHANE , DELATTRE CYRIL , ABEILLE FABIEN , KHURANA TARUN , RIVAL ARNAUD , SABOUNCHI POORYA , YUAN DAJUN , ROGERT BACIGALUPO MARIA CANDELARIA
IPC分类号： G03F7/40 , B82Y30/00 , C12Q1/68 , G01N33/52
摘要： Embodiments provided herewith are directed to self-assembled methods of preparing a patterned surface for sequencing applications including, for example, a patterned flow cell or a patterned surface for digital fluidic devices. The methods utilize photolithography to create a patterned surface with a plurality of microscale or nanoscale contours, separated by hydrophobic interstitial regions, without the need of oxygen plasma treatment during the photolithography process. In addition, the methods avoid the use of any chemical or mechanical polishing steps after the deposition of a gel material to the contours.

89. 发明专利

SG11201804563SA DIGITAL MICROFLUIDIC SYSTEM FOR SINGLE-CELL ISOLATION AND CHARACTERIZATION OF ANALYTES 未知
公开(公告)号：SG11201804563SA
公开(公告)日：2018-06-28
申请号：SG11201804563S
申请日：2016-11-30
申请人： ILLUMINA INC
发明人： JAMSHIDI ARASH , LIN YAN-YOU , ABSALAN FARNAZ , STUART SARAH , CANN GORDON , WU YIR-SHYUAN , KHURANA TARUN , FISHER JEFFREY
IPC分类号： C12Q1/68 , B01L3/00 , G01N1/28 , G01N15/10
摘要： In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

90. 发明专利

AU2016201448B2 Integrated Sequencing Apparatuses And Methods Of Use 未知
公开(公告)号：AU2016201448B2
公开(公告)日：2018-03-01
申请号：AU2016201448
申请日：2016-03-04
申请人： ILLUMINA INC
发明人： KAIN ROBERT C , KUHN KENNETH M , TALASAZ AMIRALI HAJHOSSEIN , SAKALDASIS GEORGE , VERMAAS ERIC , BOHM SEBASTIAN , KHURANA TARUN , ELTOUKHY HELMY A , GONG JIAN , SHEN MIN JUI RICHARD , JAMSHIDI ARASH
IPC分类号： B01L3/00
摘要： Provided are methods and apparatuses for performing sequencing using electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same 5 apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing. WO 2013/070627 PCT/US20 12/063741 Electrodes Liquid flow -l D Substrate mole -~A -------- detection pixels Fig. 1 Electrodes Top of flowcell Flowcell Liquid fDNA Substrate molecules ------ CMOS chip ----------. ./... ---- detection pixeVs Fig. 2 Fig. 3

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