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    • 82. 发明申请
    • Speed detection methods and devices
    • 速度检测方法和装置
    • US20060132755A1
    • 2006-06-22
    • US11085426
    • 2005-03-21
    • Dong Huang
    • Dong Huang
    • G01C3/08G01P3/36G03B13/00G03B15/02
    • G01S17/50
    • A speed detection method. A range finder acquires a specific number of distance measurement samples of an object during a period of time. A new unit of measurement larger than respective movement distances of the object in a first period and a second period is acquired. Rough measured distances D1 and D2 respectively corresponding to the locations of the object in the first period and the second period are calculated by comparing portions of the distance measurement samples corresponding to the first period and the second period utilizing the new unit of measurement. A scope is determined based on the D1 and D2 for filtering the distance measurement samples. The speed of the object is calculated based the filtered distance measurement samples.
    • 速度检测方法。 测距仪在一段时间内获取物体的特定数量的距离测量样本。 获取在第一周期和第二周期中大于对象的移动距离的新的测量单位。 通过利用新的测量单位比较与第一周期和第二周期对应的距离测量样本的部分来分别对应于第一周期和第二周期中的对象的位置的粗糙测量距离D 1和D 2。 根据用于过滤距离测量样本的D 1和D 2确定范围。 物体的速度是根据滤波后的距离测量样本计算的。
    • 83. 发明申请
    • Method for constructing and modifying large DNA molecules
    • 构建和修饰大DNA分子的方法
    • US20050084970A1
    • 2005-04-21
    • US10892579
    • 2004-07-16
    • Jian-Dong HuangXin-Mei ZhangJulian Tanner
    • Jian-Dong HuangXin-Mei ZhangJulian Tanner
    • C12N15/10C12N15/867C12P19/34
    • C12N15/10
    • This invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule.
    • 本发明提供了一种组合重叠DNA分子的方法,包括:(a)提供第一和第二DNA片段,第一DNA片段具有与第二DNA片段中的区域同源的区域; (b)用选择标记标记第一个DNA片段; (c)将第一DNA序列克隆到检索载体中以形成DNA载体复合物; (d)使DNA-载体复合物线性化; 和(e)使用同源重组将来自DNA-载体复合物的第一个DNA片段插入第二个DNA片段以形成组合的DNA分子; 和(f)除去选择性标记,从而产生组合的DNA分子。 本发明还提供了用于检索并将选定的DNA分子插入靶DNA分子的载体。