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71. 发明授权

US5911998A Method of producing a virus vaccine from an African green monkey kidney cell line 失效
标题翻译：从非洲绿猴肾细胞系生产病毒疫苗的方法
公开(公告)号：US5911998A
公开(公告)日：1999-06-15
申请号：US790598
申请日：1997-01-29
申请人： Louis Potash , Robert M. Chanock , Robert H. Purcell , Albert Z. Kapikian
发明人： Louis Potash , Robert M. Chanock , Robert H. Purcell , Albert Z. Kapikian
IPC分类号： C12N5/00 , A61K39/12 , A61K39/125 , A61K39/13 , A61K39/15 , A61K39/29 , A61P31/12 , C12N7/00 , C12N7/04
CPC分类号： C12N7/00 , A61K39/12 , A61K2039/5252 , A61K2039/5254 , C12N2720/12334 , C12N2720/12351 , C12N2740/10051 , C12N2740/14051 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2760/16251 , C12N2760/18534 , C12N2760/18551 , C12N2760/18634 , C12N2760/18651 , C12N2760/18734 , C12N2760/18751 , C12N2770/12034 , C12N2770/12051 , C12N2770/32334 , C12N2770/32351 , C12N2770/32434 , C12N2770/32451 , C12N2770/32634 , C12N2770/32651
摘要： A method for producing novel African Green Monkey Kidney (AGMK) cell lines is taught. These cell lines which are free of viable adventitious microbial agents are useful as substrates for viruses and for the preparation of viral vaccines.
摘要翻译：教导了一种生产新型非洲绿猴肾（AGMK）细胞系的方法。这些没有活的不定性微生物剂的细胞系可用作病毒的底物和用于制备病毒疫苗。

72. 发明授权

US4072565A Production of viruses in tissue culture without use of serum 失效
标题翻译：在不使用血清的情况下在组织培养物中生产病毒
公开(公告)号：US4072565A
公开(公告)日：1978-02-07
申请号：US520574
申请日：1974-11-04
申请人： Stefan A. Weiss , Harry L. Torney , William J. Caldwell
发明人： Stefan A. Weiss , Harry L. Torney , William J. Caldwell
IPC分类号： A61K39/12 , A61K39/165 , A61K39/20 , A61K39/205 , C12N5/00 , C12B3/00 , C12K9/00
CPC分类号： A61K39/165 , A61K39/12 , A61K39/20 , A61K39/205 , C12N5/0037 , C12N5/005 , C12N7/00 , A61K2039/5254 , C12N2500/34 , C12N2501/33 , C12N2760/18434 , C12N2760/18451 , C12N2760/18734 , C12N2760/18751 , C12N2770/32634 , C12N2770/32651 , C12N2770/36234 , C12N2770/36251
摘要： Growth of cells in tissue culture and virus vaccine production in tissue culture in the absence of serum are disclosed. The cells in tissue culture are grown initially in a chemically defined medium containing protamine zinc insulin, and the protamine zinc insulin medium is removed before virus propagation. Supplementation with serum or serum fractions can be eliminated entirely; and improved virus production in a serum free system can be obtained.
摘要翻译：公开了在不存在血清的组织培养物中细胞的生长和组织培养物中的病毒疫苗生产。组织培养物中的细胞最初在含有鱼精蛋白锌胰岛素的化学学定义的培养基中生长，并且在病毒传播之前除去鱼精蛋白锌胰岛素介质。补充血清或血清成分可以完全消除; 并且可以获得在无血清系统中改善的病毒产生。

73. 发明授权

US4054515A Water purification methods 失效
标题翻译：水净化方法
公开(公告)号：US4054515A
公开(公告)日：1977-10-18
申请号：US636263
申请日：1975-11-28
申请人： Edgar W. Sawyer, Jr.
发明人： Edgar W. Sawyer, Jr.
IPC分类号： C02F1/28 , C12N7/02 , C02B1/14
CPC分类号： C12N7/00 , C02F1/281 , C12N2770/32651
摘要： Treating water with various grades of attapulgite clay and sepiolite using contacting or percolation techniques removes substances not removable by standard water purification methods under many conditions. Substances such as pesticides, toxins, hormones, heavy metal cations and viruses are removed from water by adsorption upon the clay surface. When contacting is employed, the clay containing the adsorbed substances is subsequently removed by sedimentation or filtration. The clays can be regenerated by appropriate chemical or thermal techniques.
摘要翻译：使用接触或渗滤技术用各种等级的绿坡缕石和海泡石处理水，通过标准水净化方法在许多条件下去除不能除去的物质。物质如农药，毒素，激素，重金属阳离子和病毒通过吸附在粘土表面上从水中除去。当使用接触时，随后通过沉降或过滤除去含有吸附物质的粘土。粘土可以通过适当的化学或热技术再生。

74. 发明授权

US3816304A Filtering method 失效
标题翻译：过滤方法
公开(公告)号：US3816304A
公开(公告)日：1974-06-11
申请号：US19289071
申请日：1971-10-27
申请人： CARBORUNDUM CO
发明人： WALLIS C , MELNICK J
IPC分类号： B01D39/14 , B01D29/21 , C12M1/12 , C12M3/08 , C12N7/02 , C12R1/01 , C12R1/645 , C12R1/91 , B01D15/00 , B01D39/06
CPC分类号： C12N7/00 , B01D29/216 , B01D29/58 , C12N2770/32651
摘要： An annular filter element includes a contiguous layer of gel impregnated filter media substantially concentric with a center passage. To remove viruses from a liquid, the liquid is passed through the layer of gel which is formed by the method of contacting the filter media with an aqueous solution containing colloidal particles of a polyvalent metal.

75. 发明授权

US3655509A Process for the separation of virus from non-viral proteins 失效
标题翻译：从非病毒蛋白分离病毒的过程
公开(公告)号：US3655509A
公开(公告)日：1972-04-11
申请号：US3655509D
申请日：1969-05-29
申请人： MONSANTO CO
发明人： FIELDS JOSEPH E , JOHNSON JOHN H
IPC分类号： C12N7/02 , C12K7/00
CPC分类号： C12N7/00 , C12N2710/16051 , C12N2770/32651 , Y10S530/826
摘要： Viruses are selectively separated from an aqueous mixture of virus and nonviral protein by contacting the aqueous mixture with a water-insoluble polyelectrolyte polymer containing basic groups to selectively sorb the virus on the polymer and then if desired removing the polymer containing the virus from the aqueous mixture. The polyelectrolyte polymers are polycationic or polyampholytic in nature and contain imide groups selected from the group consisting of diloweralkylaminoloweralkylimide groupings and loweralkyliminodi(loweralkylimide) linkages as exemplified by crosslinked dimethylaminopropylimide derivatives of isobutylene/maleic anhydride copolymers.
摘要翻译：通过使含水混合物与含有碱性基团的水不溶性聚电解质聚合物接触，将病毒和非病毒蛋白质的水性混合物选择性地分离，以选择性地吸附聚合物上的病毒，然后如果需要，从含水混合物中除去含有病毒的聚合物。聚电解质聚合物本质上是阳离子或聚阴离子的，并且含有选自二异丁烯基氨基低级烷基亚胺基和低级烷基亚氨基（低级烷基亚胺）键的酰亚胺基，如异丁烯/马来酸酐共聚物的交联二甲基氨基丙基酰亚胺衍生物所示例。

76. 发明授权

US3197374A Process for the disaggregation and purification of viruses 失效
标题翻译：病毒分解和纯化的过程
公开(公告)号：US3197374A
公开(公告)日：1965-07-27
申请号：US23000762
申请日：1962-10-11
申请人： BEHRINGWERKE AG
发明人： WALTER HENNESSEN , HEINZ RESTLE
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , C12N2770/32651

77. 发明授权

US3147185A Process for purifying viral substances and composition 失效
标题翻译：病毒物质和组成的纯化方法
公开(公告)号：US3147185A
公开(公告)日：1964-09-01
申请号：US83954359
申请日：1959-09-10
申请人： MERCK & CO INC
发明人： JESSE CHARNEY
IPC分类号： A61K39/13 , C12N15/10
CPC分类号： C12N7/00 , A61K39/12 , A61K39/13 , C12N15/1003 , C12N2770/32634 , C12N2770/32651

78. 发明授权

US3117061A Virus suspensions and their preparation 失效
标题翻译：病毒悬浮液及其制备
公开(公告)号：US3117061A
公开(公告)日：1964-01-07
申请号：US7016160
申请日：1960-11-18
申请人： BOEHRINGER & SOEHNE GMBH
发明人： ALFRED GRAFE
IPC分类号： C12N7/02
CPC分类号： C12N7/00 , C12N2770/32651

79. 发明授权

US3097142A Benzyl alcohol stabilized polio vaccines and production thereof 失效
标题翻译：苄醇稳定的脊髓灰质炎疫苗及其生产
公开(公告)号：US3097142A
公开(公告)日：1963-07-09
申请号：US1850060
申请日：1960-03-30
申请人： MERCK & CO INC
发明人： SCHUCHARDT LEE F , MORTON ZULICK SAMUEL
IPC分类号： A61K39/13
CPC分类号： C12N7/00 , A61K39/0016 , A61K39/12 , A61K39/13 , A61K2039/70 , C12N2770/32634 , C12N2770/32651
摘要： Aqueous, killed poliomyelitis vaccines contain, as preservative, from 0.5% to 2.0% (preferably 0.5 to 0.9%) by volume of benzyl alcohol. The vaccines may contain any or all of types I, II and III poliimyelitis virus and additionally may contain one or more of diphtheria, tetanus and pertussis antigens.

80. 发明授权

US3061518A Method for the filtration of virus fluids 失效
标题翻译：病毒液过滤方法
公开(公告)号：US3061518A
公开(公告)日：1962-10-30
申请号：US82626859
申请日：1959-07-10
申请人： WILHELM AUERSWALD , JOHANN EIBL
发明人： WILHELM AUERSWALD , JOHANN EIBL
IPC分类号： B01D61/14 , B01D67/00 , C12N7/02
CPC分类号： B01D67/0088 , B01D61/145 , B01D61/18 , B01D63/00 , C12N7/00 , C12N2770/32651

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