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71. 发明授权

US07728118B2 Synthetic nucleic acid molecule compositions and methods of preparation 有权
标题翻译：合成核酸分子组合物和制备方法
公开(公告)号：US07728118B2
公开(公告)日：2010-06-01
申请号：US10943508
申请日：2004-09-17
申请人： Keith V. Wood , Monika G. Wood , Brian Almond , Aileen Paguio , Frank Fan
发明人： Keith V. Wood , Monika G. Wood , Brian Almond , Aileen Paguio , Frank Fan
IPC分类号： C07H21/02 , C07H21/04 , C12N15/00 , C12Q1/68 , C12P19/34
CPC分类号： C07K14/43595 , C07K2319/00 , C12N15/65 , C12N15/70
摘要： A method to prepare synthetic nucleic acid molecules having reduced inappropriate or unintended transcriptional characteristics when expressed in a particular host cell.
摘要翻译：制备在特定宿主细胞中表达时具有降低的不适当或非预期转录特征的合成核酸分子的方法。

72. 发明申请

US20090191622A1 SYNTHETIC NUCLEIC ACIDS FROM AQUATIC SPECIES 审中-公开
标题翻译：来自水生物种的合成核酸
公开(公告)号：US20090191622A1
公开(公告)日：2009-07-30
申请号：US12323270
申请日：2008-11-25
申请人： Brian D. Almond , Monika G. Wood , Keith V. Wood
发明人： Brian D. Almond , Monika G. Wood , Keith V. Wood
IPC分类号： C12N15/00 , C12N15/11 , C07H1/00
CPC分类号： C12N9/0069 , C07K14/43595 , C12N15/67 , C12N15/8212 , C12N15/8216
摘要： A synthetic nucleic acid molecule is provided that includes nucleotides of a coding region for a fluorescent polypeptide having a codon composition differing at more than 25% of the codons from a parent nucleic acid sequence encoding a fluorescent polypeptide. The synthetic nucleic acid molecule has at least 3-fold fewer transcription regulatory sequences relative to the average number of such sequences in the parent nucleic acid sequence. The polypeptide encoded by the synthetic nucleic acid molecule preferably has at least 85% sequence identity to the polypeptide encoded by the parent nucleic acid sequence.
摘要翻译：提供了合成核酸分子，其包括编码区域的核苷酸，所述核苷酸的密码子组成不同于编码荧光多肽的母体核酸序列的密码子的25％以上的荧光多肽。合成的核酸分子相对于母核酸序列中这样的序列的平均数目具有至少3倍的转录调节序列。由合成核酸分子编码的多肽优选与由母体核酸序列编码的多肽具有至少85％的序列同一性。

73. 发明授权

US07425436B2 Covalent tethering of functional groups to proteins and substrates therefor 有权
标题翻译：官能团共价结合到蛋白质和底物上
公开(公告)号：US07425436B2
公开(公告)日：2008-09-16
申请号：US11194110
申请日：2005-07-29
申请人： Aldis Darzins , Lance Encell , Georgyi V. Los , Keith V. Wood , Monika G. Wood , Chad Zimprich
发明人： Aldis Darzins , Lance Encell , Georgyi V. Los , Keith V. Wood , Monika G. Wood , Chad Zimprich
IPC分类号： C12N9/14 , C12N5/00 , C12N1/20 , C07K1/14 , C07K14/195 , C07K14/00 , C07C22/00 , C07H21/04
CPC分类号： G01N33/581 , C07K7/06 , C07K16/00 , C07K2317/622 , C07K2319/43 , C07K2319/61 , C09B1/00 , C09B11/24 , C09B23/04 , C09B23/06 , C09B23/105 , C12N9/14 , C12Q1/34 , C12Y308/01005 , G01N33/573 , G01N2333/914 , Y10S530/825
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译：提供任选融合到感兴趣的蛋白质的突变体水解酶。突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代野生型水解酶。还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

74. 发明授权

US06730479B2 Detection of nucleic acid hybrids 失效
标题翻译：核酸杂交体的检测
公开(公告)号：US06730479B2
公开(公告)日：2004-05-04
申请号：US09790417
申请日：2001-02-22
申请人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
发明人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
IPC分类号： C12Q168
CPC分类号： C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/68 , C12Q1/6827 , Y10T436/24 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
摘要翻译：公开了使用核酸杂交体的解聚定性和定量分析预定核酸的存在的方法。这些过程的应用包括单核苷酸多态性的检测，单碱基变化的鉴定，物种形成，病毒载量的测定，基因分型，医学标记诊断等。

75. 发明授权

US06602677B1 Thermostable luciferases and methods of production 有权
公开(公告)号：US06602677B1
公开(公告)日：2003-08-05
申请号：US09396154
申请日：1999-09-15
申请人： Keith V. Wood , Mary P. Hall
发明人： Keith V. Wood , Mary P. Hall
IPC分类号： C12Q166
CPC分类号： C12N9/0069 , C07K2319/00 , C12N15/10 , Y10S977/898
摘要： Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.

76. 发明授权

US06552179B1 Mutant luciferases 失效
公开(公告)号：US06552179B1
公开(公告)日：2003-04-22
申请号：US08478205
申请日：1995-06-07
申请人： Keith V. Wood , Monika G. Gruber
发明人： Keith V. Wood , Monika G. Gruber
IPC分类号： C12N1553
CPC分类号： C12N9/0069 , C12Y113/12007
摘要： The invention provides active, non-naturally occurring mutants of beetle luciferases and DNAs which encode such mutants. A mutant luciferase of the invention differs from the corresponding wild-type luciferase by producing bioluminescence with a wavelength of peak intensity that differs by at least 1 nm from the wavelength of peak intensity of the bioluminescence produced by the wild-type enzyme. The mutant luciferases and DNAs of the invention are employed in various biosensing applications.

77. 发明授权

US06235480B1 Detection of nucleic acid hybrids 失效
公开(公告)号：US06235480B1
公开(公告)日：2001-05-22
申请号：US09358972
申请日：1999-07-21
申请人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
发明人： John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
IPC分类号： C12Q168
CPC分类号： C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/68 , C12Q1/6827 , Y10T436/24 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

78. 发明授权

US5744320A Quenching reagents and assays for enzyme-mediated luminescence 失效
标题翻译：淬灭试剂和酶介导的发光测定
公开(公告)号：US5744320A
公开(公告)日：1998-04-28
申请号：US472546
申请日：1995-06-07
申请人： Bruce A. Sherf , Keith V. Wood , Elaine T. Schenborn
发明人： Bruce A. Sherf , Keith V. Wood , Elaine T. Schenborn
IPC分类号： C12Q1/66 , G01N21/78 , G01N33/58 , C09K11/06 , C12Q1/00 , C12Q1/37
CPC分类号： G01N33/581 , C12Q1/66 , Y10S435/963 , Y10S435/975
摘要： The present invention relates to single and dual-reporter luminescence assays utilizing general and specific reagents to quench enzyme-mediated reactions. In one embodiment of the invention, a reagent is added to the assay which non-specifically quenches enzyme-mediated luminescent reactions. In another embodiment of the invention, a reagent is added to the assay which simultaneously quenches one enzyme-mediated luminescent reaction while activating another distinct enzyme-mediated luminescent reaction. An assay kit containing specific quench reagents, and the reagents themselves are also disclosed.
摘要翻译：本发明涉及使用一般和特异性试剂来淬灭酶介导的反应的单和双重报道发光测定。在本发明的一个实施方案中，将试剂加入到非特异性淬灭酶介导的发光反应的测定中。在本发明的另一个实施方案中，将试剂加入到测定中，同时淬灭一种酶介导的发光反应，同时激活另一种不同的酶介导的发光反应。还公开了包含特定淬灭试剂的测定试剂盒和试剂本身。

79. 发明授权

US5641641A Kit for luciferase assay 失效
标题翻译：荧光素酶测定试剂盒
公开(公告)号：US5641641A
公开(公告)日：1997-06-24
申请号：US485834
申请日：1995-06-07
申请人： Keith V. Wood
发明人： Keith V. Wood
IPC分类号： G01N21/76 , C12N9/02 , C12Q1/66 , G01N33/53
CPC分类号： C12N9/0069 , C12Q1/66 , C12Y113/12007 , Y10S435/81 , Y10S435/968 , Y10S435/975 , Y10S436/808
摘要： Methods and compositions are provided for improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions. Thus, the invention provides methods, compositions and test kits for assaying samples for the presence of a beetle luciferase or, using a beetle luciferase-luciferin reaction, the presence of ATP. The invention also provides the complex of the coenzyme, Coenzyme A, a beetle luciferase and oxyluciferin in its exited state in the luciferase-luciferin reaction and luciferyl-CoA, the thioester of Coenzyme A and luciferin (D-(-)-2-(6'-hydroxy -2'-benzothiazolyl)-.DELTA..sup.2 -thiazoline-4-carboxylic acid).
摘要翻译：提供了方法和组合物，用于改善甲虫荧光素酶 - 荧光素反应中荧光素酶活性的光产生动力学。因此，本发明提供了用于测定样品中存在甲虫荧光素酶的方法，组合物和测试试剂盒，或者使用甲虫荧光素酶 - 荧光素反应存在ATP。本发明还提供辅酶A，甲壳素萤光素酶和荧光素酶荧光素酶在荧光素酶荧光素反应中的氧化荧光素与荧光素-CoA的络合物，辅酶A的硫酯和荧光素（D - （ - ） - 2- 6'-羟基-2'-苯并噻唑基） - DELTA 2-噻唑啉-4-羧酸）。

80. 发明授权

US09018014B2 Vectors for directional cloning 有权
标题翻译：用于定向克隆的载体
公开(公告)号：US09018014B2
公开(公告)日：2015-04-28
申请号：US13274643
申请日：2011-10-17
申请人： Michael R. Slater , Keith V. Wood , James Robert Hartnett
发明人： Michael R. Slater , Keith V. Wood , James Robert Hartnett
IPC分类号： C12N15/74 , C12N15/10 , C12N15/63 , C12N15/64 , C12N15/65 , C12N15/66 , C12N15/73 , G06F19/28
CPC分类号： C12N15/73 , C12N15/10 , C12N15/63 , C12N15/64 , C12N15/65 , C12N15/66 , G06F19/28
摘要： The invention provides vectors and methods for directional cloning.
摘要翻译：本发明提供用于定向克隆的载体和方法。

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