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71. 发明授权

US06280937B1 Shuttle vectors 有权
标题翻译：班车向量
公开(公告)号：US06280937B1
公开(公告)日：2001-08-28
申请号：US09133944
申请日：1998-08-14
申请人： Ying Luo , Pei Wen Yu , James Lorens
发明人： Ying Luo , Pei Wen Yu , James Lorens
IPC分类号： C12Q168
CPC分类号： C12N15/1082 , C12N15/70 , C12N15/81
摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.
摘要翻译：本发明提供穿梭载体，以及使用能够在至少哺乳动物细胞中表达的穿梭载体的方法。此外，穿梭载体能够在至少酵母和任选的细菌细胞中复制。还提供了一种方法，其中用本文提供的穿梭载体转化酵母。将与穿梭载体内的同源重组位点相同的5'和3'末端的异源核酸引入转化的酵母中，并与穿梭载体同源重组，使得它们被酵母生物体插入载体中。然后将穿梭载体回收并转移至哺乳动物细胞进行表达。

72. 发明授权

US6114111A Mammalian protein interaction cloning system 失效
标题翻译：哺乳动物蛋白质相互作用克隆系统
公开(公告)号：US6114111A
公开(公告)日：2000-09-05
申请号：US50863
申请日：1998-03-30
申请人： Ying Luo , Betty Huang , Donald Payan
发明人： Ying Luo , Betty Huang , Donald Payan
IPC分类号： C12N15/10 , C12N15/12 , C12Q1/70
CPC分类号： C12N15/1055 , C12Q2565/201
摘要： The present invention is directed to compositions and methods for a genetic system of detecting protein--protein interactions in a mammalian host cell. Two fusion proteins are made in the host cell. The first fusion protein contains a DNA binding domain which is fused to a so-called bait protein. The second fusion protein consists of a transcriptional activation domain fused to a so-called test protein. The transcriptional activation domain is recruited to the promoter through the functional interaction between the bait protein and the test protein. Subsequently the transcriptional activation domain interacts with the basal transcription machinery to activate expression of one or more reporter genes which can be identified and characterized. The individual compositions are useful for analyzing protein--protein interactions between known proteins and to isolate, clone and characterize unknown proteins. The individual compositions can be used to express the fusion proteins either transiently or stably.
摘要翻译：本发明涉及用于在哺乳动物宿主细胞中检测蛋白质 - 蛋白质相互作用的遗传系统的组合物和方法。在宿主细胞中制备两种融合蛋白。第一个融合蛋白含有与所谓诱饵蛋白融合的DNA结合结构域。第二种融合蛋白由与所谓的测试蛋白融合的转录激活结构域组成。通过诱饵蛋白质和测试蛋白质之间的功能相互作用将转录激活结构域引入启动子。随后，转录激活结构域与基础转录机制相互作用以激活一种或多种可鉴定和表征的报告基因的表达。单独的组合物可用于分析已知蛋白质之间的蛋白质 - 蛋白质相互作用并分离，克隆和表征未知蛋白质。单独的组合物可用于瞬时或稳定地表达融合蛋白。

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