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    • 71. 发明申请
    • Process for producing a decaffeinated coffee plant by genetic recombination
    • 通过遗传重组生产脱咖啡因咖啡植物的方法
    • US20080127373A1
    • 2008-05-29
    • US10521244
    • 2005-01-14
    • Shinjiro OgitaHiroshi SanoNozomu KoizumiAtsuhiko Shinmyo
    • Shinjiro OgitaHiroshi SanoNozomu KoizumiAtsuhiko Shinmyo
    • A01H5/00
    • C12N15/8243A01H5/00C12N9/1007
    • The invention provides a process for producing a decaffeinated coffee plant by genetic recombination comprising a step for preparing an antisense sequence or an RNAi sequence of a gene coding for an enzyme related to the caffeine biosynthetic pathway and constructing an expression vector for transformation, a step for introducing the obtained expression vector into Agrobacterium, a step for infecting a cell division-activated tissue piece of a coffee plant or a callus or an adventitious embryo induced from a tissue piece of a coffee plant with the Agrobacterium to transform the tissue piece, the callus or the adventitious embryo and a step for obtaining an transformed coffee plant from a transformed tissue piece, a transformed callus or a transformed adventitious embryo so that a coffee plant with low caffeine content can be produced by suppressing expression of a gene coding for an enzyme related to the caffeine biosynthetic pathway by the antisense method or the RNAi method.
    • 本发明提供了一种通过遗传重组生产脱咖啡因咖啡植物的方法,包括制备编码与咖啡因生物合成途径相关的酶的基因的反义序列或RNAi序列的步骤,并构建用于转化的表达载体的步骤 将获得的表达载体导入农杆菌中,用于感染咖啡植物或愈伤组织的细胞分裂激活的组织片或由土壤杆菌从咖啡植物的组织块诱导的不定胚以转化组织块,愈伤组织 或不定胚,以及从转化的组织片,转化愈伤组织或转化的不定胚获得转化的咖啡植物的步骤,从而可以通过抑制编码相关酶的基因的表达来产生咖啡因含量低的咖啡植物 通过反义方法或RNAi方法转化为咖啡因生物合成途径。
    • 75. 发明授权
    • Biological fluorescence diagnostic apparatus with distinct pickup cameras
    • 具有不同拾取相机的生物荧光诊断仪
    • US5772580A
    • 1998-06-30
    • US607361
    • 1996-02-27
    • Tetsuya UtsuiHiroshi SanoRensuke Adachi
    • Tetsuya UtsuiHiroshi SanoRensuke Adachi
    • A61B1/00A61B1/04A61B5/00G02B23/24H04N7/18
    • H04N7/183A61B1/00186A61B1/043A61B1/0638A61B1/0646A61B5/0071A61B5/0084G02B23/2469
    • A biological fluorescence diagnostic apparatus having a device for irradiating a biological tissue with light which excites the tissue to generate fluorescent light, and a device for taking a fluorescence image of the biological tissue passing through an ocular optical system of an endoscope. The apparatus further has a television camera unit including a television camera for taking an ordinary endoscopic observation image passing through the ocular optical system, and a television camera with an image intensifier for taking a fluorescence observation image passing through the ocular optical system after amplifying the light intensity of the image. An optical path switching system which includes a reflective surface is selectively inserted and withdrawn from the optical path of light passing through the ocular optical system so as to selectively produce ordinary and fluorescence images in the television camera unit. A filter is selectively inserted into and movable out of an illuminating light path of the endoscope in response to detection of the position of the optical path switching system.
    • 一种生物荧光诊断装置,其具有利用激发组织以产生荧光的光照射生物体组织的装置,以及摄取通过内窥镜的眼睛光学系统的生物体的荧光图像的装置。 该装置还具有电视摄像机单元,其包括用于摄取通过眼睛光学系统的普通内窥镜观察图像的电视摄像机,以及具有图像增强器的电视摄像机,用于在放大光线后拍摄通过眼睛光学系统的荧光观察图像 图像的强度。 包括反射表面的光路切换系统从通过眼睛光学系统的光的光路中选择性地插入和退出,以便在电视摄像机单元中选择性地产生普通和荧光图像。 响应于光路切换系统的位置的检测,滤光器被选择性地插入并移出内窥镜的照明光路。