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    • 80. 发明授权
    • Methods for quenching pathogen inactivators in biological materials
    • 在生物材料中淬灭病原体灭活剂的方法
    • US06709810B2
    • 2004-03-23
    • US09912031
    • 2001-07-23
    • David CookAdonis Stassinopoulos
    • David CookAdonis Stassinopoulos
    • A01N102
    • A61L2/0088
    • Methods are provided for quenching undesired side reactions of pathogen inactivating compounds in biological materials. In a particular embodiment, methods are provided for quenching undesired side reactions of a pathogen inactivating compound that includes a functional group which is, or which is capable of forming, an electrophilic group. In this embodiment, the material is treated with the pathogen inactivating compound and a quencher, wherein the quencher comprises a nucleophilic functional group that is capable of covalently reacting with the electrophilic group. The electrophilic group on the pathogen inactivating compound is preferably a non-radical cationic group. In one embodiment, the pathogen inactivating compound includes a nucleic acid binding ligand and a mustard group, wherein the mustard group is capable of reacting in situ to form the electrophilic group. Preferred quenchers are thiols, such as glutathione. Biological materials which may be treated include whole blood, red blood cells, blood plasma, and platelets. The methods permit inhibition of the modification of red blood cells in red blood cell containing materials during pathogen inactivation.
    • 提供了用于猝灭病原体灭活化合物在生物材料中的不希望的副反应的方法。 在一个具体实施方案中,提供了用于猝灭病原体灭活化合物的不希望的副反应的方法,其包括可亲电子基团或能够形成亲电基团的官能团。 在该实施方案中,用病原体灭活化合物和猝灭剂处理材料,其中猝灭剂包含能够与亲电子基团共价反应的亲核官能团。 病原体灭活化合物上的亲电基团优选为非自由基阳离子基团。 在一个实施方案中,病原体灭活化合物包括核酸结合配体和芥子基团,其中芥末基团能够在原位反应形成亲电子基团。 优选的猝灭剂是硫醇,如谷胱甘肽。 可以治疗的生物材料包括全血,红细胞,血浆和血小板。 该方法允许在病原体灭活期间抑制含红细胞的材料中的红细胞的修饰。