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71. 发明申请

US20050158755A1 Spotting device for manufacturing DNA microarray and spotting method using the same 失效
标题翻译：用于制造DNA微阵列的检测装置及使用其的点样方法
公开(公告)号：US20050158755A1
公开(公告)日：2005-07-21
申请号：US11011017
申请日：2004-12-10
申请人： Jeong-gun Lee , Hun-joo Lee
发明人： Jeong-gun Lee , Hun-joo Lee
IPC分类号： C12Q1/68 , B01J19/00 , B01L3/02 , C40B40/06 , C40B40/10 , C40B60/14 , G01N35/00 , G01N35/10 , B41J2/01 , C12M1/34
CPC分类号： B01L3/0244 , B01J19/0046 , B01J2219/00367 , B01J2219/00369 , B01J2219/00529 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01L3/0248 , B01L3/0268 , B01L2200/0673 , B01L2300/1827 , B01L2300/1894 , B01L2400/02 , B01L2400/0475 , B01L2400/0633 , B82Y30/00 , C40B40/06 , C40B40/10 , C40B60/14 , G01N35/1074 , G01N2035/00158 , G01N2035/00237 , G01N2035/1034 , Y10T436/2575
摘要： A spotting device for manufacturing a DNA microarray and a spotting method using the same are provided. The spotting device for dropping and immobilizing a solution of biomolecules, for example nucleic acids such as probe DNA, mRNA, and peptide nucleic acid (PNA), and proteins on a DNA microarray surface to manufacture a DNA microarray, includes a first microchannel with a tube shape; a supplying unit supplying the solution of biomolecules to the first microchannel; a biomolecule solution droplet forming unit cross-linked to the first microchannel and forming biomolecule solution droplets with a predetermined size by periodically jetting a gas toward the biomolecule solution flowing in the first microchannel; a second microchannel linked to the first microchannel and having a greater diameter than the first microchannel; a cooling unit surrounding at least a part of the second microchannel to freeze the biomolecule solution droplets which pass through the second microchannel; and a spotting unit thawing the frozen biomolecule solution droplets and dropping the thawed biomolecule solution droplets on a surface of the DNA microarray. The spotting device can form spots with uniform shape, minimize an effect of temperature on biomolecules, and easily manipulate biomolecules when manufacturing a DNA microarray.
摘要翻译：提供了用于制造DNA微阵列的点样装置和使用其的点样方法。用于将生物分子溶液（例如探针DNA，mRNA和肽核酸（PNA）等核酸）和蛋白质滴入和固定在DNA微阵列表面上以制备DNA微阵列的点样装置包括具有管形; 供应单元，将生物分子的溶液供应到第一微通道; 生物分子溶液液滴形成单元，其与所述第一微通道交联并通过向在所述第一微通道中流动的生物分子溶液周期性地喷射气体而以预定尺寸形成生物分子溶液液滴; 连接到第一微通道并且具有比第一微通道更大的直径的第二微通道; 围绕第二微通道的至少一部分的冷却单元，以冷冻通过第二微通道的生物分子溶液液滴; 以及将冷冻的生物分子溶液液解冻并将解冻的生物分子溶液滴落在DNA微阵列的表面上的点样单元。点样装置可以形成具有均匀形状的斑点，最小化温度对生物分子的影响，并且在制造DNA微阵列时容易操纵生物分子。

72. 发明申请

US20050136459A1 Patch for microarray reaction chamber having adhesive means support and two or more adhesive materials 审中-公开
标题翻译：用于具有粘合剂装置的微阵列反应室的补片和两种或更多种粘合剂材料
公开(公告)号：US20050136459A1
公开(公告)日：2005-06-23
申请号：US10985155
申请日：2004-11-10
申请人： Jeong-gun Lee , Hun-joo Lee
发明人： Jeong-gun Lee , Hun-joo Lee
IPC分类号： G01N33/53 , B01L3/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N1/28 , G01N37/00 , G02B21/34 , C12M1/34
CPC分类号： B29C65/5057 , B01L3/508 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , B01L2300/0887 , B29C65/5021 , B29C65/76 , B29C66/1122 , B29C66/53461 , B29C66/54 , B29C66/71 , B29C2793/0009 , B29L2031/756 , G01N2001/2833 , G02B21/34 , B29K2083/00 , B29K2069/00 , B29K2067/003 , B29K2067/00 , B29K2033/12 , B29K2025/06 , B29K2023/12 , B29K2023/06
摘要： Provided is a patch for a reaction chamber of a microarray, which includes a support means provided with a sample inlet and an adhesive means adhering to the support means and providing an adhesive strength capable of being attached to a microarray substrate, wherein the adhesive means has stacked adhesive means supports having both surfaces to which an adhesive material is deposited and a first adhesive material located between the adhesive means support and the adhesive means support or between the adhesive means support and the support means has 33 oz/in greater adhesive strength (according to ASTM D3330 Method), based on an adhesive strength to steel, than a second adhesive material located between the patch and the microarray substrate.
摘要翻译：提供了一种用于微阵列反应室的贴片，其包括设置有样品入口的支撑装置和粘附到支撑装置上并提供能够附着到微阵列基底的粘合强度的粘合剂，其中粘合剂装置具有堆叠的粘合剂装置支撑体具有沉积粘合剂材料的两个表面，并且位于粘合装置支撑件和粘合装置之间的第一粘合剂材料支撑或粘合装置支撑件和支撑装置之间具有33盎司/英寸以上的粘合强度（根据与ASTM D3330方法相比），基于与钢的粘合强度比位于贴片和微阵列基片之间的第二粘合剂材料。

73. 发明授权

US09238225B2 Fluid controlling apparatus and method of controlling fluid by using the same 有权
标题翻译：流体控制装置及其使用方法
公开(公告)号：US09238225B2
公开(公告)日：2016-01-19
申请号：US13571202
申请日：2012-08-09
申请人： June-young Lee , Sang-hyun Baek , Hyo-young Jeong , Tae-seok Sim , Jeong-gun Lee
发明人： June-young Lee , Sang-hyun Baek , Hyo-young Jeong , Tae-seok Sim , Jeong-gun Lee
IPC分类号： G01N1/20 , B01L3/00 , G01N35/00 , G01N35/04
CPC分类号： B01L3/502753 , B01L3/5027 , B01L2200/0621 , B01L2200/141 , B01L2300/0681 , B01L2300/0861 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , G01N2035/00237 , G01N2035/0437 , Y10T137/4259
摘要： A fluid controlling apparatus including at least one sample chamber for holding a fluid containing target materials; a cleaning chamber for holding a cleaning solution; a first multi-port connected to the at least one sample chamber through a first channel and connected to the cleaning chamber through a second channel; a filter portion, connected to the first multi-port through a third channel, for filtering the target materials; and a first pump, connected to the filter portion, for applying a pressure; and a method of controlling a fluid using the fluid controlling apparatus, which comprises passing the fluid containing the target materials from the at least one sample chamber to the filter portion; and cleaning a path of the fluid by passing the cleaning solution through the path.
摘要翻译：一种流体控制装置，包括用于保持含有目标材料的流体的至少一个样品室; 用于保持清洁溶液的清洁室; 第一多端口，其通过第一通道连接到所述至少一个样品室，并通过第二通道连接到所述清洁室; 过滤器部分，通过第三通道连接到第一多端口，用于过滤目标材料; 以及连接到过滤器部分的用于施加压力的第一泵; 以及使用所述流体控制装置控制流体的方法，所述方法包括将包含所述目标材料的流体从所述至少一个样品室传送到所述过滤器部分; 以及通过使清洁溶液通过路径来清洁流体的路径。

74. 发明授权

US08557568B2 Method and apparatus for disrupting cells and amplifying nucleic acids using gold nanorods 有权
标题翻译：使用金纳米棒破坏细胞和扩增核酸的方法和装置
公开(公告)号：US08557568B2
公开(公告)日：2013-10-15
申请号：US13107677
申请日：2011-05-13
申请人： Kwang Ho Cheong , Dong-kee Yi , Jeong-gun Lee , Jong-myeon Park
发明人： Kwang Ho Cheong , Dong-kee Yi , Jeong-gun Lee , Jong-myeon Park
IPC分类号： C12M1/34 , C12M3/00 , C12M1/00
CPC分类号： C12N1/066 , C12M47/06 , C12N13/00 , C12P19/34
摘要： Provided is a method of disrupting cells comprising adding gold nanorods to a solution containing cells and irradiating the gold nanorods with a laser to disrupt the cells. A method and an apparatus for continuously disrupting cells and amplifying nucleic acids in a single microchamber are also provided, wherein the method comprises introducing a solution containing cells and gold nanorods into a microchamber, irradiating a laser onto the gold nanorods to disrupt the cells, and amplifying a nucleic acid from the disrupted cells in the microchamber. The apparatus comprises a cell disruption chamber comprising a sample inlet, and gold nanorods introduced therein; a laser attached to the cell disruption chamber, wherein the laser is for generating light at a wavelength absorbed by the gold nanorods; and a heater and a cooler for heating and cooling the cell disruption chamber. Also disclosed is a lab-on-a chip comprising the apparatus.
摘要翻译：提供一种破坏细胞的方法，包括向含有细胞的溶液中加入金纳米棒并用激光照射金纳米棒以破坏细胞。还提供了用于在单个微室中连续破坏细胞和扩增核酸的方法和装置，其中所述方法包括将含有细胞和金纳米棒的溶液引入微室中，将激光照射到金纳米棒上以破坏细胞，以及在微室中从破碎的细胞中扩增核酸。该装置包括一个细胞破碎室，包括一个样品入口和引入其中的金纳米棒; 附着到细胞破碎室的激光器，其中所述激光器用于产生由所述金纳米棒吸收的波长的光; 以及用于加热和冷却细胞破碎室的加热器和冷却器。还公开了一种包括该装置的芯片实验室。

75. 发明授权

US08464760B2 Valve unit, reaction apparatus with the same, and method of forming valve in channel 有权
标题翻译：阀门单元，具有相同的反应装置，以及在通道中形成阀的方法
公开(公告)号：US08464760B2
公开(公告)日：2013-06-18
申请号：US11770762
申请日：2007-06-29
申请人： Jong-myeon Park , Jeong-gun Lee
发明人： Jong-myeon Park , Jeong-gun Lee
IPC分类号： F15C1/04
CPC分类号： F16K99/0032 , B01L3/502738 , B01L2300/0806 , B01L2300/087 , B01L2300/0887 , B01L2300/1861 , B01L2400/0677 , F16K99/0001 , F16K99/004 , F16K2099/0084 , Y10T29/49405 , Y10T137/2196
摘要： A valve unit, a reaction apparatus with the same, and a method of forming a valve by injecting a valve material into a channel are provided. The valve unit comprises a fluid channel constituting a flow path of a fluid and having a portion containing a first area of a first dimension (“D1”) and a second area of a second dimension (“D2”), wherein D1
摘要翻译：提供一种阀单元，具有该阀单元的反应装置以及通过将阀材料注入通道而形成阀的方法。阀单元包括构成流体的流路的流体通道，并具有包含第一维度（“D1”）和第二区域（“D2”）的第一区域的部分，其中D1

76. 发明授权

US08367422B2 Biochemical analyzer and method of controlling internal temperature of the biochemical analyzer 有权
标题翻译：生化分析仪和生化分析仪内部温度控制方法
公开(公告)号：US08367422B2
公开(公告)日：2013-02-05
申请号：US13081193
申请日：2011-04-06
申请人： Jeong-gun Lee , Yong-moo Shin , Kui-hyun Kim , Jin-han Chung
发明人： Jeong-gun Lee , Yong-moo Shin , Kui-hyun Kim , Jin-han Chung
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48
CPC分类号： B01L7/52 , B01L3/5027 , B01L9/527 , B01L2300/0681 , B01L2300/0806 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2300/1883 , B01L2400/0409 , G01N35/00069 , G01N2035/00346 , Y10T436/11 , Y10T436/25
摘要： Provided is a biochemical analyzer including: a microfluidic device loading space including a microfluidic device supporting unit detachably supporting a microfluidic device including an electromagnetic radiation application region in which electromagnetic energy is applied; an energy source loading space including an energy source applying the electromagnetic energy to the electromagnetic radiation application region; and an isolation wall isolating the microfluidic device loading space and the energy source loading space to prevent heat transfer between the microfluidic device loading space and the energy source loading space and including a transparent window through which the electromagnetic energy can be transmitted. A method of controlling an internal temperature of the biochemical analyzer is also provided.
摘要翻译：提供了一种生物化学分析仪，其包括：微流体装置装载空间，包括微流体装置支撑单元，其可拆卸地支撑包括施加电磁能的电磁辐射施加区域的微流体装置; 能量源装载空间，其包括将电磁能施加到电磁辐射施加区域的能量源; 以及隔离壁，隔离微流体装置加载空间和能量源装载空间，以防止微流体装置加载空间和能量源装载空间之间的热传递，并且包括可透射电磁能的透明窗口。还提供了一种控制生化分析仪内部温度的方法。

77. 发明授权

US07429470B2 Method for purification of nucleic acids by phase separation using laser and beads 有权
标题翻译：使用激光和珠子通过相分离纯化核酸的方法
公开(公告)号：US07429470B2
公开(公告)日：2008-09-30
申请号：US11265461
申请日：2005-11-02
申请人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim
发明人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim
IPC分类号： C12N13/00 , C12N1/08 , G01N33/00 , C07H21/02
CPC分类号： B01L3/502761 , B01L3/502738 , B01L7/52 , B01L2200/0668 , B01L2300/0838 , B01L2300/087 , B01L2400/0633 , G01N35/0098 , Y10T436/143333
摘要： An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.
摘要翻译：提供了用于纯化细胞或病毒核酸的装置和方法。核酸纯化装置包括：细胞裂解毛细管，其具有样品和磁珠引入的样品入口; 连接到毛细管并将样品和磁珠混合在毛细管中的振动器; 激光发生器，附接到毛细管并向毛细管提供激光; 以及附接到毛细管并将磁珠固定到毛细管壁的磁力发生器。根据该方法和装置，可以增加PCR产量，因为通过毛细管中的相分离可以容易地除去PCR抑制剂。使用电磁铁确保去除PCR抑制剂。此外，由于可以同时进行细胞裂解和DNA纯化过程，因此可以减少LOC步骤。

78. 发明授权

US07416705B2 Spotting device for manufacturing DNA microarray and spotting method using the same 失效
标题翻译：用于制造DNA微阵列的检测装置及使用其的点样方法
公开(公告)号：US07416705B2
公开(公告)日：2008-08-26
申请号：US11011017
申请日：2004-12-10
申请人： Jeong-gun Lee , Hun-joo Lee
发明人： Jeong-gun Lee , Hun-joo Lee
IPC分类号： B01L3/02 , G01N1/10
CPC分类号： B01L3/0244 , B01J19/0046 , B01J2219/00367 , B01J2219/00369 , B01J2219/00529 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01L3/0248 , B01L3/0268 , B01L2200/0673 , B01L2300/1827 , B01L2300/1894 , B01L2400/02 , B01L2400/0475 , B01L2400/0633 , B82Y30/00 , C40B40/06 , C40B40/10 , C40B60/14 , G01N35/1074 , G01N2035/00158 , G01N2035/00237 , G01N2035/1034 , Y10T436/2575
摘要： A spotting device for manufacturing a DNA microarray and a spotting method using the same are provided. The spotting device for dropping and immobilizing a solution of biomolecules, for example nucleic acids such as probe DNA, mRNA, and peptide nucleic acid (PNA), and proteins on a DNA microarray surface to manufacture a DNA microarray, includes a first microchannel with a tube shape; a supplying unit supplying the solution of biomolecules to the first microchannel; a biomolecule solution droplet forming unit cross-linked to the first microchannel and forming biomolecule solution droplets with a predetermined size by periodically jetting a gas toward the biomolecule solution flowing in the first microchannel; a second microchannel linked to the first microchannel and having a greater diameter than the first microchannel; a cooling unit surrounding at least a part of the second microchannel to freeze the biomolecule solution droplets which pass through the second microchannel; and a spotting unit thawing the frozen biomolecule solution droplets and dropping the thawed biomolecule solution droplets on a surface of the DNA microarray. The spotting device can form spots with uniform shape, minimize an effect of temperature on biomolecules, and easily manipulate biomolecules when manufacturing a DNA microarray.
摘要翻译：提供了用于制造DNA微阵列的点样装置和使用其的点样方法。用于将生物分子溶液（例如探针DNA，mRNA和肽核酸（PNA）等核酸）和蛋白质滴入和固定在DNA微阵列表面上以制备DNA微阵列的点样装置包括具有管形; 供应单元，将生物分子的溶液供应到第一微通道; 生物分子溶液液滴形成单元，其与所述第一微通道交联并通过向在所述第一微通道中流动的生物分子溶液周期性地喷射气体而以预定尺寸形成生物分子溶液液滴; 连接到第一微通道并且具有比第一微通道更大的直径的第二微通道; 围绕第二微通道的至少一部分的冷却单元，以冷冻通过第二微通道的生物分子溶液液滴; 以及将冷冻的生物分子溶液液解冻并将解冻的生物分子溶液滴落在DNA微阵列的表面上的点样单元。点样装置可以形成具有均匀形状的斑点，最小化温度对生物分子的影响，并且在制造DNA微阵列时容易操纵生物分子。

79. 发明申请

US20080042096A1 VALVE UNIT, REACTION APPARATUS WITH THE SAME, AND METHOD OF FORMING VALVE IN CHANNEL 有权
标题翻译：阀门单元，反应装置及其在通道中形成阀的方法
公开(公告)号：US20080042096A1
公开(公告)日：2008-02-21
申请号：US11770762
申请日：2007-06-29
申请人： Jong-myeon Park , Jeong-gun Lee
发明人： Jong-myeon Park , Jeong-gun Lee
IPC分类号： F16K51/00
CPC分类号： F16K99/0032 , B01L3/502738 , B01L2300/0806 , B01L2300/087 , B01L2300/0887 , B01L2300/1861 , B01L2400/0677 , F16K99/0001 , F16K99/004 , F16K2099/0084 , Y10T29/49405 , Y10T137/2196
摘要： A valve unit, a reaction apparatus with the same, and a method of forming a valve by injecting a valve material into a channel are provided. The valve unit comprises a fluid channel constituting a flow path of a fluid and having a portion containing a first area of a first dimension (“D1”) and a second area of a second dimension (“D2”), wherein D1
摘要翻译：提供一种阀单元，具有该阀单元的反应装置以及通过将阀材料注入通道而形成阀的方法。阀单元包括构成流体流动通道的流体通道，并具有包含第一维度（“D 1”）和第二区域（“D 2”）的第一区域的部分，其中D 1

80. 发明申请

US20060094051A1 Apparatus and method for purification of nucleic acids by phase separation using laser and beads 有权
标题翻译：使用激光和珠子通过相分离纯化核酸的装置和方法
公开(公告)号：US20060094051A1
公开(公告)日：2006-05-04
申请号：US11265461
申请日：2005-11-02
申请人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim
发明人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim
IPC分类号： C12Q1/68 , C12N1/08 , C12M1/34
CPC分类号： B01L3/502761 , B01L3/502738 , B01L7/52 , B01L2200/0668 , B01L2300/0838 , B01L2300/087 , B01L2400/0633 , G01N35/0098 , Y10T436/143333
摘要： An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.
摘要翻译：提供了用于纯化细胞或病毒核酸的装置和方法。核酸纯化装置包括：细胞裂解毛细管，其具有样品和磁珠引入的样品入口; 连接到毛细管并将样品和磁珠混合在毛细管中的振动器; 激光发生器附接到毛细管并向毛细管提供激光; 以及附接到毛细管并将磁珠固定到毛细管壁的磁力发生器。根据该方法和装置，可以增加PCR产量，因为通过毛细管中的相分离可以容易地除去PCR抑制剂。使用电磁铁确保去除PCR抑制剂。此外，由于可以同时进行细胞裂解和DNA纯化过程，因此可以减少LOC步骤。

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