专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

71. 发明申请

US20060057139A1 Process for producing humanized chimera antibody 审中-公开
公开(公告)号：US20060057139A1
公开(公告)日：2006-03-16
申请号：US11228293
申请日：2005-10-25
申请人： Kenya Shitara , Nobuo Hanai , Mamoru Hasegawa , Hiromasa Miyaji , Yoshihisa Kuwana
发明人： Kenya Shitara , Nobuo Hanai , Mamoru Hasegawa , Hiromasa Miyaji , Yoshihisa Kuwana
IPC分类号： A61K39/395 , C07H21/04 , C12P21/06 , C12N5/06
CPC分类号： C07K16/3084 , A61K38/00 , A61K2039/505 , C07K16/18 , C07K16/462 , C07K2317/24 , C07K2317/732 , C07K2317/734 , C07K2319/00 , C07K2319/02
摘要： A humanized chimera antibody, a pharmaceutical composition comprising a humanized chimera antibody and a pharmaceutically acceptable carrier, and a method of treating cancer which comprises administering to a patient a pharmaceutically acceptable amount of the humanized chimera antibody, are disclosed.

72. 发明授权

US06759231B2 Phage with nuclear localization signal 失效
标题翻译：噬菌体与核定位信号
公开(公告)号：US06759231B2
公开(公告)日：2004-07-06
申请号：US09844813
申请日：2001-04-27
申请人： Mahito Nakanishi , Emi Nagoshi , Teruo Akuta , Katsuo Takeda , Mamoru Hasegawa
发明人： Mahito Nakanishi , Emi Nagoshi , Teruo Akuta , Katsuo Takeda , Mamoru Hasegawa
IPC分类号： C12N120
CPC分类号： C12N15/1037 , C12N15/85 , C12N15/86 , C12N15/87 , C12N2795/10343
摘要： A &lgr; phage with a nuclear localization signal has been obtained by constructing a vector capable of expressing a fused protein between a gpD protein constituting the head of a &lgr; phage and a nuclear localization signal sequence, transforming Escherichia coli with this vector, and propagating a mutant &lgr; phage which cannot express the gpD protein in E. coli in this transformant. It has been confirmed that the resulting &lgr; phage is capable of packaging &lgr; phage DNAs of 80% and 100% genome sizes. After further confirming that the nuclear localization signal exposed on the outside of the head of this phage, this phage has been microinjected into cells to analyze its nuclear localization activity. Thus, it has been clarified that this phage has a nuclear localization activity.
摘要翻译：已经通过构建能够在构成λ噬菌体的头部的gpD蛋白质和核定位信号序列之间表达融合蛋白质的载体，用该载体转化大肠杆菌并扩增突变体来获得具有核定位信号的λ噬菌体 λ噬菌体在该转化体中不能在大肠杆菌中表达gpD蛋白。已经证实所得的λ噬菌体能够包装80％和100％基因组大小的λ噬菌体DNA。在进一步证实核定位信号暴露在该噬菌体头部的外部时，该噬菌体已被微量注入细胞以分析其核定位活性。因此，已经澄清这种噬菌体具有核定位活动。

73. 发明授权

US06716608B1 Artificial chromosome 失效
标题翻译：人造染色体
公开(公告)号：US06716608B1
公开(公告)日：2004-04-06
申请号：US09254947
申请日：2000-03-13
申请人： Fuyuki Ishikawa , Mamoru Hasegawa
发明人： Fuyuki Ishikawa , Mamoru Hasegawa
IPC分类号： C12P1934
CPC分类号： C12N9/1241 , C12N15/81 , C12N15/85 , C12N2800/206 , C12N2830/002 , C12N2830/702
摘要： An artificial mammalian chromosome, more specifically, a clone containing a mammalian centromere sequence and a DNA replication origin with mammalian telomere sequences added to both ends of the clone, is provided by preparing a CEPH artificial yeast chromosome library containing a human genome, identifying clones having a repetitive human alphoid sequence from this library, and further preparing a yeast strain in which mammalian telomere sequences are added to the ends of its chromosome.
摘要翻译：通过制备含有人类基因组的CEPH人工酵母染色体文库，鉴定了克隆含有人源哺乳动物染色体，更具体地，含有哺乳动物着丝粒序列和DNA复制起点的克隆，其中所述克隆含有哺乳动物端粒序列，来自该文库的重复的人类亚型序列，并进一步制备其中将哺乳动物端粒序列加入其染色体的末端的酵母菌株。

74. 发明授权

US06225459B1 TEC promoter 失效
公开(公告)号：US06225459B1
公开(公告)日：2001-05-01
申请号：US09142529
申请日：1999-08-12
申请人： Hiroyuki Mano , Tsuneaki Sakata , Mamoru Hasegawa
发明人： Hiroyuki Mano , Tsuneaki Sakata , Mamoru Hasegawa
IPC分类号： C07H2104
CPC分类号： C12N9/1205 , C12N15/85 , C12N2830/00 , C12N2830/85
摘要： The present invention provides a DNA having promoter activity of Tec tyrosine kinase and a vector having incorporated within it the promoter to thereby enable a high level expression of an exogenous gene in hematopoietic stem cells and hepatic cells.

75. 发明授权

US5866692A Process for producing humanized chimera antibody 失效
公开(公告)号：US5866692A
公开(公告)日：1999-02-02
申请号：US454680
申请日：1995-05-31
申请人： Kenya Shitara , Nobuo Hanai , Mamoru Hasegawa , Hiromasa Miyaji , Yoshihisa Kuwana
发明人： Kenya Shitara , Nobuo Hanai , Mamoru Hasegawa , Hiromasa Miyaji , Yoshihisa Kuwana
IPC分类号： A61K36/00 , A61K38/00 , A61P35/00 , C07K7/06 , C07K7/08 , C07K14/00 , C07K16/00 , C07K16/18 , C07K16/30 , C07K16/32 , C07K16/46 , C07K19/00 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/12 , C12N15/13 , C12N15/62 , C12P21/08 , C12R1/91 , C12N15/11
CPC分类号： C07K16/3084 , C07K16/18 , C07K16/462 , A61K2039/505 , A61K38/00 , C07K2317/24 , C07K2317/732 , C07K2317/734 , C07K2319/00 , C07K2319/02
摘要： A humanized chimera antibody, a pharmaceutical composition comprising a humanized chimera antibody and a pharmaceutically acceptable carrier, and a method of treating cancer which comprises administering to a patient a pharmaceutically acceptable amount of said humanized chimera antibody, are disclosed.

76. 发明授权

US5830470A Humanized antibodies to ganglioside GM.sub.2 失效
公开(公告)号：US5830470A
公开(公告)日：1998-11-03
申请号：US116778
申请日：1993-09-07
申请人： Kazuyasu Nakamura , Masamichi Koike , Kenya Shitara , Nobuo Hanai , Yoshihisa Kuwana , Mamoru Hasegawa
发明人： Kazuyasu Nakamura , Masamichi Koike , Kenya Shitara , Nobuo Hanai , Yoshihisa Kuwana , Mamoru Hasegawa
IPC分类号： A61K38/00 , C07K16/18 , C07K16/46 , C12N1/21 , C12N15/13 , C12P21/08 , A61K39/395 , C07H21/04 , C12P21/04
CPC分类号： C07K16/3084 , C07K16/18 , C07K16/464 , A61K38/00 , C07K2317/24 , C07K2317/732 , C07K2317/734 , C07K2319/00 , C07K2319/035 , Y10S530/867
摘要： Chimeric human antibody expression vectors are constructed by inserting the antibody heavy chain variable region-encoding cDNA and antibody light chain variable region-encoding cDNA isolated from hybridomas producing a mouse or rat monoclonal antibody reacting with the ganglioside GM.sub.2 respectively into an expression vector for use in animal cells which contains the human antibody heavy chain constant region- or human antibody light chain constant region-encoding cDNA. The expression vectors are introduced into animal cells and the transformant thus obtained is cultured for the production of a chimeric human antibody reacting with the ganglioside GM.sub.2. In contrast to mouse monoclonal antibodies, the chimeric human antibodies of the invention will not cause anti-mouse immunoglobulin antibody production in the patient's body but shows a prolonged blood half-life, with a reduced frequency of adverse effects, so that it can be expected to be superior to mouse monoclonal antibodies in the efficacy in the treatment of human cancer, for instance.

77. 发明授权

US5268290A Process for producing neuraminidase 失效
标题翻译：生产神经氨酸酶的方法
公开(公告)号：US5268290A
公开(公告)日：1993-12-07
申请号：US384709
申请日：1989-07-25
申请人： Mamoru Hasegawa , Kazuhiro Sakurada
发明人： Mamoru Hasegawa , Kazuhiro Sakurada
IPC分类号： A61K38/00 , C12N1/21 , C12N9/24 , C12N15/09 , C12N15/56 , C12R1/29 , C12R1/465 , C12N9/26 , C12N15/76
CPC分类号： C12Y302/01018 , C12N9/2402 , A61K38/00
摘要： Streptomyces lividans transformed with a recombinant DNA containing a DNA fragment which is derived from Micromonospora viridifaciens and which comprises a DNA sequence encoding a neuraminidase as well as flanking sequence regions is cultured, and neuraminidase can be efficiently recovered from the culture. Neuraminidase is utilized for clinical diagnosis of inflammatory diseases, diffuse collagen diseases, cancer, etc.
摘要翻译：用含有源自小单孢菌的DNA片段的重组DNA转化并含有编码神经氨酸酶以及侧翼序列区的DNA序列的变色青链霉菌进行培养，从培养物中有效地回收神经氨酸酶。神经氨酸酶用于炎性疾病，弥漫性胶原病，癌症等的临床诊断。

78. 发明申请

US20190194607A1 HIGHLY FUNCTIONAL LIVER CELLS DERIVED FROM PLURIPOTENT STEM CELLS, METHOD FOR PRODUCING SAME, AND METHOD FOR TESTING METABOLISM/TOXICITY OF DRUG 审中-公开
公开(公告)号：US20190194607A1
公开(公告)日：2019-06-27
申请号：US13982661
申请日：2012-01-30
申请人： Akira Yuo , Kumiko Saeki , Naoko Nakamura , Satoko Matsuyama , Miwako Nishio , Koichi Saeki , Mamoru Hasegawa
发明人： Akira Yuo , Kumiko Saeki , Naoko Nakamura , Satoko Matsuyama , Miwako Nishio , Koichi Saeki , Mamoru Hasegawa
IPC分类号： C12N5/071 , C12Q1/02
CPC分类号： C12N5/067 , C12N5/0672 , C12N2500/90 , C12N2501/115 , C12N2501/155 , C12N2501/16 , C12N2501/237 , C12N2501/39 , C12N2501/41 , C12N2501/415 , C12N2506/02 , C12N2506/45 , C12Q1/025 , G01N33/5014 , G01N33/5067
摘要： The problem is to produce functional liver cells usable in testing the metabolism and toxicity of a drug, from pluripotent stem cells. The solution includes a method for producing highly functional liver cells using pluripotent stem cells, comprising, from pluripotent stem cells, acquiring primitive endoderm derived from the pluripotent stem cells by a process involving steps (A) and (B), from the primitive endoderm, acquiring liver precursor cells by a process involving step (C), and, from the liver precursor cells, acquiring the highly functional liver cells by a process involving step (D): (A) culturing under serum-free and feeder-free conditions; (B) culturing in the presence of albumin and at least one kind of cytokine; (C) culturing in the presence of SHH or an SHH agonist and at least one kind of cytokine; and (D) culturing and maturing in the presence of at least one kind of cytokine.

79. 发明授权

US08381387B2 Method of fabricating an ultrasonic transducer 有权
标题翻译：制造超声波换能器的方法
公开(公告)号：US08381387B2
公开(公告)日：2013-02-26
申请号：US12914651
申请日：2010-10-28
申请人： Kazuya Matsumoto , Ryo Ohta , Mamoru Hasegawa , Hideo Adachi , Katsuhiro Wakabayashi
发明人： Kazuya Matsumoto , Ryo Ohta , Mamoru Hasegawa , Hideo Adachi , Katsuhiro Wakabayashi
IPC分类号： H04R31/00 , H04R17/00 , H01L41/22
CPC分类号： A61B8/4488 , A61B8/12 , A61B8/445 , B06B1/0292 , Y10T29/42 , Y10T29/49005 , Y10T29/49155
摘要： An ultrasonic transducer fabrication method including: depositing a conductive material on an insulating layer, partially etching the conductive material to form lower electrodes; depositing an insulating material to cover the lower electrodes to form a first insulating layer and depositing a sacrificial material thereon, performing etching, to create cavities and a channel-shaped sacrificial layer to communicate the cavities; depositing an insulating material on the first insulating layer to form a second insulating layer; partially etching the second insulating layer to form holes; etching and removing the sacrificial layer through the holes to form the cavities and channels; depositing a conductive material on the second insulating layer to plug the holes and form a conductive film; partially etching the conductive film to form upper electrodes and sealing portions which plug the holes; and forming a protective film on the second insulating layer to cover the upper electrodes and the sealing portions.
摘要翻译：一种超声换能器制造方法，包括：在绝缘层上沉积导电材料，部分地蚀刻导电材料以形成下电极; 沉积绝缘材料以覆盖下电极以形成第一绝缘层并在其上沉积牺牲材料，执行蚀刻以产生空腔和沟槽状牺牲层以连通空腔; 在所述第一绝缘层上沉积绝缘材料以形成第二绝缘层; 部分蚀刻第二绝缘层以形成孔; 通过孔蚀刻和去除牺牲层以形成空腔和通道; 在第二绝缘层上沉积导电材料以堵塞孔并形成导电膜; 部分地蚀刻导电膜以形成上电极和堵塞孔的密封部分; 以及在所述第二绝缘层上形成保护膜以覆盖所述上电极和所述密封部。

80. 发明授权

US08345512B2 Capacitive micromachined ultrasonic transducer (cMUT) device and method of controlling the same 有权
标题翻译：电容式微加工超声波换能器（cMUT）装置及其控制方法
公开(公告)号：US08345512B2
公开(公告)日：2013-01-01
申请号：US12062240
申请日：2008-04-03
申请人： Hideo Adachi , Katsuhiro Wakabayashi , Kosei Tamiya , Masaaki Amikura , Kazuya Matsumoto , Ryo Ohta , Mamoru Hasegawa , Hiroshi Ito
发明人： Hideo Adachi , Katsuhiro Wakabayashi , Kosei Tamiya , Masaaki Amikura , Kazuya Matsumoto , Ryo Ohta , Mamoru Hasegawa , Hiroshi Ito
IPC分类号： A61B8/00 , B06B1/02 , H01L41/09
CPC分类号： A61B8/00 , A61B8/12 , B06B1/0207 , B06B1/0292 , B06B2201/51 , G01N29/2406
摘要： A capacitive micromachined ultrasonic transducer (cMUT) device, comprising: a cMUT formed on a semiconductor substrate; a DC high-voltage generation unit that is provided on the semiconductor substrate and that is for generating a DC high-voltage signal to be superposed on a driving signal for the cMUT; a driving signal generation unit that is provided on the semiconductor substrate and that is for generating the driving signal; and a superposition unit that is provided on the semiconductor substrate and that is for branching the DC high-voltage signal output from the DC high-voltage generation unit and for superposing one of the branched DC high-voltage signals on the other of the branched DC high-voltage signals via the driving signal generation unit.
摘要翻译：一种电容微加工超声波换能器（cMUT）装置，包括：形成在半导体衬底上的cMUT; DC高电压产生单元，设置在半导体基板上，用于产生叠加在cMUT的驱动信号上的DC高电压信号; 驱动信号生成单元，其设置在所述半导体基板上，用于产生所述驱动信号; 以及叠加单元，其设置在所述半导体基板上，并且用于分支从所述直流高压发生单元输出的所述直流高电压信号，并且用于将所述分支直流高电压信号中的一个叠加在所述分支DC 通过驱动信号发生单元的高电压信号。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式