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71. 发明公开

KR1020020032753A 혈중 콜레스테롤의 농도 측정 방법 및 이를 위한 측정용키트 无效
标题翻译：用于测量血液中胆固醇浓度的方法和测量试剂盒
公开(公告)号：KR1020020032753A
公开(公告)日：2002-05-04
申请号：KR1020000063385
申请日：2000-10-27
申请人： 김희태
发明人： 김희태 , 윤용달
IPC分类号： G01N33/538
摘要： PURPOSE: A method for measuring cholesterol concentration and measuring kit for the same is provided to achieve improved accuracy of analysis result and reduce time and costs for analysis. CONSTITUTION: A method comprises a first step of mixing the blood sample with a solution, and removing blood cell by passing the mixture through a filter; a second step of injecting the blood sample into a pipe which is branched off to four capillary pipes; a third step of shifting the blood to the capillary pipes with a non-treated membrane, a membrane which is treated with an antibody with respect to a low concentration lipoprotein, a membrane which is treated with an antibody with respect to a high concentration lipoprotein, and a membrane treated with both antibodies, such that only the target lipoprotein passes through the capillary pipe by the immune reaction with antibody; a fourth step of shifting blood cell samples passed through the capillary pipe, to a membrane treated with a surface active agent, and permitting cholesterol component to be dissolved from the lipoprotein in the sample; a fifth step of shifting the solution obtained in the fourth step to membranes of each capillary pipe treated with cholesterol decomposition enzyme and color developing agent so as to permit the color developing agent to be dissolved and cholesterol to react with the enzyme; a sixth step of shifting the reaction solution to the reaction space where the enzyme for the color developing agent is fixed; and a seventh step of checking cholesterol concentration for each capillary pipe by measuring the degree of color development.
摘要翻译：目的：提供一种测定胆固醇浓度的方法和测量套件，以提高分析结果的准确性，并缩短分析的时间和成本。构成：一种方法包括将血液样品与溶液混合的第一步骤，以及通过使混合物通过过滤器去除血细胞; 将血液样本注入分支到四个毛细管的管中的第二步骤; 用未经处理的膜将血液转移到毛细管的第三步骤，用低浓度脂蛋白用抗体处理的膜，用抗体对高浓度脂蛋白进行处理的膜，和用两种抗体处理的膜，使得只有目标脂蛋白通过与抗体的免疫反应通过毛细管; 将通过毛细管的血细胞样品转移到用表面活性剂处理的膜并允许胆固醇成分从样品中的脂蛋白溶解的第四步骤; 将第四步骤中获得的溶液转移到用胆固醇分解酶和显色剂处理的每个毛细管的膜上，以使得显色剂溶解并使胆固醇与酶反应的第五步骤; 将反应溶液转移到固色用于显色剂的酶的反应空间的第六步骤; 以及通过测量显色程度来检查每个毛细管的胆固醇浓度的第七步骤。

72. 发明公开

KR1020020032751A 지지체로서 니트로셀룰로스 멤브레인을 사용한 마이크로웰 플레이트 단백질 칩 및 이의 제조 방법 无效
标题翻译：使用N-纤维素膜的MICROWELL板蛋白芯片作为支持并生产其方法
公开(公告)号：KR1020020032751A
公开(公告)日：2002-05-04
申请号：KR1020000063383
申请日：2000-10-27
申请人： 김희태
发明人： 김희태
IPC分类号： C07K17/12
CPC分类号： G01N33/548 , G01N33/68
摘要： PURPOSE: A microwell plate protein chip using nitrocellulose membrane as a support and a producing method thereof are provided, therefore the protein can be easily fixed on the surface and a larger number of sample can be simultaneously analyzed. CONSTITUTION: The microwell plate protein chip contains the nitrocellulose membrane support fixing at least one kind of proteins, such as antigens or antibodies, capable of specifically binding with proteins to be analyzed, to its surface and of which rear side is coated with a polystyrene, polypropylene or polyethylene solution. The method for producing the microwell plate protein chip comprises the steps of: coating the rear side of the nitrocellulose membrane with hydrophobic polymer solution; cutting the nitrocellulose membrane to be inserted into each well of the microwell plate; sticking the nitrocellulose on the bottom of each well of the microwell plate; spraying 0.05 to 0.5 microliter of at least one kind of antigens or antibodies capable of specifically binding with a desired protein on the surface of the nitrocellulose membrane; and reacting them at 35 to 40 deg.C for 1 hour.
摘要翻译：目的：提供使用硝酸纤维素膜作为载体的微孔板蛋白芯片及其制备方法，因此蛋白质可以容易地固定在表面上，并且可以同时分析更多的样品。构成：微孔板蛋白质芯片含有硝酸纤维素膜支持物，其将至少一种能够与要分析的蛋白质特异性结合的蛋白质（例如抗原或抗体）固定到其表面，并且其后侧涂覆有聚苯乙烯，聚丙烯或聚乙烯溶液。微孔板蛋白芯片的制造方法包括以下步骤：用疏水聚合物溶液涂覆硝酸纤维素膜的后侧; 切割硝基纤维素膜以插入微孔板的每个孔中; 将硝化纤维素粘附在微孔板的每个孔的底部; 喷洒0.05至0.5微升至少一种能够在硝酸纤维素膜表面上与所需蛋白特异性结合的抗原或抗体; 并在35〜40℃反应1小时。

73. 发明授权

KR100309740B1 두부제조용응고제및이응고제를이용한두부의제조방법 有权
标题翻译：用于制造豆腐的凝固剂和使用该凝结剂生产豆腐的方法
公开(公告)号：KR100309740B1
公开(公告)日：2001-12-17
申请号：KR1019980039320
申请日：1998-09-22
申请人： 김희태
发明人： 김희태
IPC分类号： A23L1/20
摘要： 본 발명은 종래의 방법과는 달리, 두유를 반드시 냉각을 할 필요는 없이 염화마그네슘을 주제로 한 응고제 및 이 응고제를 사용하여 풍미, 탄력, 수율등이 우수한 두부를 용이하게 제조하는 방법을 제공하는 것으로, 본 발명에 따른 응고제는 속효성 응고제 10-90중량부, 지효성 응고제 0-10중량부, 유지 10-60중량부, 유화제 0-10중량부 및 물 0-30중량부를 상온 내지 섭씨100도의 온도에서 교반, 혼합하여 제조되며, 본 발명에 따른 응고제를 사용함으로써 두부 제조시 응고속도를 자유롭게 조절할 수 있게 된다.

74. 发明公开

KR1020010070898A 생리 혈액을 이용하여 질병을 진단할 수 있게 하여주는면역 자가진단기 无效
标题翻译：使用卡塔米尼亚的自身疾病诊断试剂盒和自我诊断疾病的方法
公开(公告)号：KR1020010070898A
公开(公告)日：2001-07-27
申请号：KR1020010034413
申请日：2001-06-18
申请人： 김희태
发明人： 김희태
IPC分类号： G01N33/558
摘要： PURPOSE: A self disease-diagnosis kit article using katamenia and a method for self diagnosing diseases are provided, thereby a user can easily and rapidly diagnose several diseases himself without additional blood sampling process. CONSTITUTION: The method for self diagnosing diseases comprises the steps of: collecting katamenia using a sanitary napkin; recovering an absorbing stick from the sanitary napkin; inserting the absorbing stick into the self disease-diagnosis kit article; transferring the katamenia into a pad from the stick; moving the katamenia to an analysing membrane via a hemocyte separating-membrane and a conjugate pad; forming bands produced by antibody specific binding between antibody in the membrane and the components of katamenia; and identifying diseases by analysing the bends. The self disease-diagnosing apparatus using katamenia consists of a sanitary napkin and a katamenia absorbing stick.
摘要翻译：目的：提供使用katamenia的自身疾病诊断试剂盒文章和自我诊断疾病的方法，从而用户可以自己轻松快速诊断几种疾病，无需额外的血液采样过程。构成：自我诊断疾病的方法包括以下步骤：使用卫生巾收集消化不良; 从卫生巾中回收吸收棒; 将吸收棒插入自身疾病诊断试剂盒中; 将katamenia从棍子转移到垫中; 通过血细胞分离膜和共轭垫将katamenia移动到分析膜; 通过抗体在膜中的抗体与katamenia的成分之间的抗体特异性结合产生的形成带; 并通过分析弯曲来识别疾病。使用katamenia的自身疾病诊断装置由卫生巾和katamenia吸收棒组成。

75. 发明公开

KR1020010070809A 인체의 질환을 진단할 수 있는 간편 진단용 멤브레인 면역크로마토그래프 스트립을 여성용 생리대에 부착하여생리혈액으로 질병을 진단하는 방법 无效
标题翻译：使用卡塔米尼亚自我诊断疾病的方法
公开(公告)号：KR1020010070809A
公开(公告)日：2001-07-27
申请号：KR1020010032197
申请日：2001-06-08
申请人： 김희태
发明人： 김희태
IPC分类号： G01N33/53
摘要： PURPOSE: A method for self diagnosing diseases using katamenia is provided for a user to easily and rapidly diagnose several diseases without additional blood sampling process. CONSTITUTION: The method for self diagnosing diseases comprises the steps of: fixing the second antibody for the desired diseases onto a membrane strip; adhering a gold conjugate treated pad containing the first antigen or antibody; adhering a hemocyte removing pad to the front of the conjugate pad; sealing the membrane and the pad with specific pas or clothe to produce a self disease diagnosing-kit article; collecting katamenia using a sanitary napkin; passing only serum solution through the conjugate pad by removing hemocyte from the katamenia with the hemocyte removing pad; binding the first antigen or antibody with the components of the katamenia by the specific immunological binding reaction; binding the serum components with the second antibody on the membrane; forming bends at the position where the second antibody reacts with the serum components; and determining disease by analysing the bends.
摘要翻译：目的：使用katamenia自诊断疾病的方法可供用户轻松快速诊断多种疾病，无需额外的采血过程。构成：自诊断疾病的方法包括以下步骤：将所需疾病的第二抗体固定在膜条上; 粘附含有第一抗原或抗体的金结合物处理垫; 将血细胞去除垫粘附到缀合垫的前面; 用特定的pas或衣服密封膜和垫以产生自身疾病诊断试剂盒物品; 使用卫生巾收集katamenia; 只有血清溶液通过缀合物垫，通过用血细胞去除垫从洗涤剂中除去血细胞; 通过特异性免疫结合反应将第一抗原或抗体与katamenia的成分结合; 将血清组分与第二抗体结合在膜上; 在第二抗体与血清成分反应的位置处形成弯曲部; 并通过分析弯曲来确定疾病。

76. 发明公开

KR1020010069271A 인터넷을 이용한 도서 보상판매방법 无效
标题翻译：使用互联网的书籍的补偿营销方法
公开(公告)号：KR1020010069271A
公开(公告)日：2001-07-25
申请号：KR1020010009720
申请日：2001-02-26
申请人： 김희태 , 김보영
发明人： 김희태 , 김보영
IPC分类号： G06Q30/06
摘要： PURPOSE: A book compensation marketing method for books using the Internet is provided to make a client read a book promptly and sell the book again by providing all sorts of information with respect to a book to a client and selling the book and deciding a compensation payment at a returning the book in accordance with a returning time and the state of the book. CONSTITUTION: If a client inputs a book searching request(S202), the requested book is searched in a new book database and a used book database(S203). The searching result is transmitted to the client(S204). If the client inserts the book in a shopping bag and requests a purchase of the book, the corresponding client checks whether the state is a log-in state(S206). If the state is not a log-in state, a joining to the members or a log-in process are performed(S207). If the state is a log-in state, an order sheet is prepared(S208), and a paying method is decided(S209). If a purchase history list inquiry request is received from the client(S202), it is checked whether the corresponding client performs a log-in(S210). If the state is not a log-in state, a log-in process are performed(S211). If the state is a log-in state, a purchase history list and a return books list are displayed on a web screen of the client(S212). The client requests a return of the book by clicking a return requesting button(S213). The server system receives a return requesting and moves a compensation payment to a client's account.
摘要翻译：目的：提供使用互联网的图书补偿营销手段，让客户迅速阅读书籍，并向客户提供有关书籍的各种信息，并出售书籍并作出赔偿付款。根据返回的时间和书的状态返回书。构成：如果客户端输入图书搜索请求（S202），则在新图书数据库和旧书数据库中搜索所请求的书籍（S203）。搜索结果被发送到客户端（S204）。如果客户端将书籍插入购物袋并请求购买书籍，相应的客户端将检查该状态是否为登录状态（S206）。如果状态不是登录状态，则执行对成员的加入或登录处理（S207）。如果状态是登录状态，则准备订单（S208），并且确定付款方式（S209）。如果从客户端接收到购买历史列表查询请求（S202），则检查相应的客户端是否执行登录（S210）。如果状态不是登录状态，则执行登录处理（S211）。如果状态是登录状态，则在客户端的网络屏幕上显示购买历史列表和返回图书列表（S212）。客户端通过单击返回请求按钮来请求返回书籍（S213）。服务器系统接收请求并将补偿支付移交给客户的帐户。

77. 发明公开

KR1020000072201A 여러 종류의 디엔에이 프로브들을 사용한 취약-엑스증후군의 진단 방법 无效
标题翻译：使用各种DNA探针的脆性X综合征的诊断方法
公开(公告)号：KR1020000072201A
公开(公告)日：2000-12-05
申请号：KR1020000047468
申请日：2000-08-17
申请人： 김희태
发明人： 김희태
IPC分类号： C12Q1/68
摘要： PURPOSE: Provided is a diagnosis method of Fragile-X-syndrome using various DNA probes, which bind with nucleic acid sequence of FMR1 gene related to fragile-X syndrome. Fragile-X-syndrome is diagnosed accurately in a short time by detecting repeat frequency of CGN(n) and CGG sequences in FMR1 gene of the nucleic acid specimen. CONSTITUTION: A diagnosis method of Fragile-X-syndrome using various DNA probes is characterized by comprising the following steps of: (i)decomposing nucleic acid specimens by treating with DNA restriction enzyme which specifically cleaves the environs of FMR1 gene; (ii)adding DNA probe of sequence CGG(m) and GCC(m) whereto the first label material is adhered(m is 3-10 of integer, the times whose CGG and GCC are repeated) and followed by mixing; (iii)adding 2 kinds of sense and anti-sense DNA probes whereto the second label material capable of binding with internal sequence of FMR1 gene is adhered and followed by mixing; (iv)adding 2 kinds of sense and anti sense DNA probes capable of binding with internal sequence of FMR1 gene, whereto biotin is adhered and followed by mixing; (v)thermal denaturation by heating the nucleic acid associated DNA probes; (vi)reacting so as to bind the denatured nucleic acid with DNA probes specifically; (vii)fixing nucleic acid associated DNA probe on micro well plate by injecting into micro well plate where streptavidin is immobilized on the surface; (viii)removing non binding nucleic acid and DNA probe by washing micro well plate; (ix)measuring the nucleic acid amount which is fixed on micro well plate by reading the second label material; (x)measuring the repeat sequence CGG(n) in nucleic acid specimen by reading the first label material; (xi)enumerating the CGG repeat number n in nucleic acid specimen from the result of (ix) and (x).
摘要翻译：目的：提供使用各种DNA探针的脆性-X综合征的诊断方法，其与与脆性-X综合征相关的FMR1基因的核酸序列结合。通过检测核酸标本的FMR1基因中CGN（n）和CGG序列的重复频率，在短时间内精确诊断易碎X综合征。构成：使用各种DNA探针的脆性-X综合征的诊断方法的特征在于包括以下步骤：（i）通过用特异性切割FMR1基因周围的DNA限制酶处理来分解核酸标本; （ii）添加序列CGG（m）和GCC（m）的DNA探针，粘附第一标签材料（m为整数3-10，重复CGG和GCC的时间），然后混合; （iii）加入2种有义和反义DNA探针，其中能够与FMR1基因的内部序列结合的第二标记物质粘附并随后混合; （iv）加入2种能与FMR1基因的内部序列结合的有义和反义DNA探针，生物素粘附在其上，然后混合; （v）加热核酸相关DNA探针的热变性; （vi）反应以使变性核酸与DNA探针具体结合; （vii）将微孔板上的核酸相关DNA探针通过注射到表面固定有链霉抗生物素蛋白的微孔板中; （viii）通过洗涤微孔板除去非结合核酸和DNA探针; （ix）通过读取第二标签材料测量固定在微孔板上的核酸量; （x）通过读取第一标签材料测量核酸试样中的重复序列CGG（n）; （i）从（ix）和（x）的结果列举核酸标本中的CGG重复数n。

78. 外观设计

KR3020000001357S 无效
公开(公告)号：KR3020000001357S
公开(公告)日：2000-08-16
申请号：KR3020000005744
申请日：2000-03-08
申请人： 김희태
设计人： 김희태

79. 实用新型

KR2019980058110U 투시도 러프 스케치북(PERSPECTIVE LOUGH SKETCH BOOK) 无效
标题翻译：透视粗糙的草图书
公开(公告)号：KR2019980058110U
公开(公告)日：1998-10-26
申请号：KR2019970002473
申请日：1997-02-19
申请人： 김희태
发明人： 김희태
IPC分类号： B42D1/00
摘要： 본 고안은 건축설계, 실내장식설계, 가구디자인, 도시계획 등 입체적인 창작활동의 초기 행위인 러프스케치(아이디어 구상을 입체적인 이미지로 화지에 옮기는 행위로써 거칠고 간략하게 표현하는 기법)를 쉽게 익히고 활용할 수 있게 하는데 목적이 있다.
투시도 작도를 용이케 하는 그리드 안내선을 허선에 가깝게 인쇄하여 형태를 잡는데 드는 시간소모를 극소화하고 투시도 기법 습득의 기간을 단축하여 실제 설계자가 다양한 아이디어 창출함에 있어 효과적인 사무용품이 되도록 하였다.

80. 外观设计

KR3000766150000S 失效
公开(公告)号：KR3000766150000S
公开(公告)日：1987-11-30
申请号：KR3019860013295
申请日：1986-09-15
申请人： 김희태
设计人： 김희태

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