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71. 发明申请

WO2013103370A1 METHODS AND COMPOSITIONS FOR MODULATING EXPRESSION OR ACTIVITY OF A RKD POLYPEPTIDE IN A PLANT 审中-公开
标题翻译：用于调节植物中RKD多肽的表达或活性的方法和组合物
公开(公告)号：WO2013103370A1
公开(公告)日：2013-07-11
申请号：PCT/US2012/033326
申请日：2012-04-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , CHAMBERLIN, Mark A. , LAWIT, Shai J.
发明人： CHAMBERLIN, Mark A. , LAWIT, Shai J.
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8233 , C07K14/415 , C12N15/8261 , C12N15/8287
摘要： Methods and compositions are provided to increase the activity/level of an RKD polypeptide or an active variant or fragment thereof in an unreduced ovule plant cell that is outside of the embryo sac. In specific embodiments, such modulation of activity/level of the RKD polypeptide promotes an egg cell-like state in an unreduced ovule plant cell that is outside of the embryo sac. Such methods and compositions can employ an expression construct comprising a RKD polypeptide or active variant or fragment thereof operably linked to an ovule tissue-preferred promoter, in particular an ovule tissue-preferred promoter that is active in at least one non-gametophyte tissue in a plant ovule and is active in an unreduced cell that is outside of the embryo sac.
摘要翻译：提供方法和组合物以增加RKD多肽或其在胚囊外的未还原胚珠植物细胞中的活性变体或片段的活性/水平。在具体实施方案中，RKD多肽的活性/水平的这种调节促进在胚囊外的未还原胚珠植物细胞中的卵细胞样状态。这样的方法和组合物可以使用包含RKD多肽或其活性变体或其片段的表达构建体，其可操作地连接到胚珠组织优选启动子，特别是在至少一种非配子体组织中有活性的胚珠组织优选启动子植物胚珠，并且在胚胎囊外的未减少的细胞中是活跃的。

72. 发明申请

WO2013101750A1 METHODS OF IMPROVING APHID RESISTANCE IN SOYBEANS 审中-公开
标题翻译：提高大豆抗性的方法
公开(公告)号：WO2013101750A1
公开(公告)日：2013-07-04
申请号：PCT/US2012/071328
申请日：2012-12-21
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ALT, Jessie L. , CHAKY, Julian M. , RYAN-MAHMUTAGIC, Molly , WOODWARD, John B.
IPC分类号： A01H1/00 , A01H1/04 , A01H5/10 , C12Q1/68
CPC分类号： A01H1/04 , A01H1/00 , A01H1/02 , A01H5/10 , C12N15/8286 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： This invention relates to methods of improving resistance to aphids in soybean plants, as well as methods for identifying and/or selecting soybean plants or germplasm that display improved resistance to one or more biotypes of soybean aphid. In certain examples, the method comprises selecting a first and second soybean plant or germplasm, each of which has a different favorable Rag1, Rag2, or Rag3, allele, haplotype, or marker profile, and crossing those first and second soybean plants to produce a progeny plant with improved soybean aphid resistance. This invention further relates to markers, primers, probes, kits, systems, etc., useful for carrying out the methods described herein.
摘要翻译：本发明涉及改善大豆植物中对蚜虫抗性的方法，以及用于鉴定和/或选择显示对一种或多种大豆蚜虫生物型的改良抗性的大豆植物或种质的方法。在某些实例中，该方法包括选择第一种和第二种大豆植物或种质，每种大豆植物或种质均具有不同的有利的Rag1，Rag2或Rag3等位基因，单倍型或标记特征，并且与第一和第二大豆植物杂交以产生具有改良大豆蚜虫抗性的后代植物。本发明还涉及可用于实施本文所述方法的标记，引物，探针，试剂盒，系统等。

73. 发明申请

WO2013096562A1 USE OF THE SOYBEAN SUCROSE SYNTHASE PROMOTER TO INCREASE PLANT SEED LIPID CONTENT 审中-公开
标题翻译：使用大豆蔗糖合成酶促进剂增加植物种子脂肪含量
公开(公告)号：WO2013096562A1
公开(公告)日：2013-06-27
申请号：PCT/US2012/070828
申请日：2012-12-20
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL, INC.
发明人： DAMUDE, Howard, Glenn , DAINES, Bryce , MEYER, Knut , RIPP, Kevin, G. , STECCA, Kevin, L.
IPC分类号： C12N15/82 , C12N9/10
CPC分类号： C12N15/8247 , C07K14/415 , C12N9/1062 , C12N15/8218 , C12N15/8234
摘要： Recombinant DNA constructs comprising the soybean sucrose synthase promoter operably linked to polynucleotides encoding transcription factors such as ODP1, Lec1 and FUSCA3 are disclosed. These constructs are used for increasing oil content while maintaining normal germination in oilseed plants. Methods to increase oil content in the seeds of an oilseed plant using this construct are also disclosed herein.
摘要翻译：公开了包含与编码转录因子如ODP1，Lec1和FUSCA3的多核苷酸可操作地连接的大豆蔗糖合成酶启动子的重组DNA构建体。这些构建体用于增加油含量，同时保持油籽植物的正常发芽。本文还公开了使用该构建体增加油籽植物种子中油含量的方法。

74. 发明申请

WO2013070854A1 METHOD FOR DISPENSING GRAINS OF POLLEN 审中-公开
标题翻译：分配花粉颗粒的方法
公开(公告)号：WO2013070854A1
公开(公告)日：2013-05-16
申请号：PCT/US2012/064068
申请日：2012-11-08
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： COPE, Jason M. , KRONE, Todd L. , MCAVOY, Bradley T.
IPC分类号： A01H1/02
CPC分类号： A01G7/00 , A01H1/02
摘要： Methods for dispensing pollen contained in a solution onto a plant for pollination are provided herein. In one example embodiment, a method for dispensing grains of pollen comprises introducing a plurality of grains of pollen into a solution to create a pollen solution mixture. In some embodiments, the pollen solution mixture is contained in a pollen storage container. The method further comprises connecting the pollen storage container containing the pollen solution mixture to a dispensing device. The method further includes propelling at least a portion of the pollen solution mixture from the pollen storage container using the dispensing device to pollinate a plant.
摘要翻译：本发明提供将溶液中所含的花粉分配到植物上进行授粉的方法。在一个示例性实施例中，一种用于分配花粉颗粒的方法包括将多个花粉颗粒引入溶液中以产生花粉溶液混合物。在一些实施方案中，将花粉溶液混合物包含在花粉储存容器中。该方法还包括将含有花粉溶液混合物的花粉储存容器连接到分配装置。该方法还包括使用分配装置从花粉储存容器推进至少一部分花粉溶液混合物以授粉植物。

75. 发明申请

WO2013063487A1 METHODS AND COMPOSITIONS FOR SILENCING GENES USING ARTIFICIAL MICRORNAS 审中-公开
标题翻译：使用人造微生物沉默基因的方法和组合物
公开(公告)号：WO2013063487A1
公开(公告)日：2013-05-02
申请号：PCT/US2012/062249
申请日：2012-10-26
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL
发明人： KUREK, Itzhak , MCGONIGLE, Brian , ZHU, Genhai
IPC分类号： C12N15/63 , C12N15/82
CPC分类号： C07K14/415 , C12N9/14 , C12N9/88 , C12N15/8218 , C12N15/8269 , C12Y306/01 , C12Y401/01031
摘要： Methods and compositions are provided that employ microRNA (miRNA) that, when expressed in a plant cell, is capable of reducing the level of mRNA of a target sequence (i.e. endogenous sequence) without reducing the level of mRNA of one or more closely related sequences. While miRNAs can be designed with specificity for a particular target sequence, the instant application demonstrates that a miRNA can specifically silence a target sequence without silencing a closely related sequence having high sequence identity to the target sequence. In certain embodiments, an endogenous target sequence can be suppressed with a recombinant miRNA expression construct without silencing a recombinant polynucleotide of interest having a sequence closely related to the target sequence. Such methods and compositions employ recombinant miRNA expression constructs which produce a 21-nt miRNA. Transgenic plant cells, plants and seeds incorporating miRNA expression constructs and recombinant polynucleotide constructs comprising polynucleotides of interest are also provided.
摘要翻译：提供了使用微小RNA（miRNA）的方法和组合物，其在植物细胞中表达时能够降低靶序列（即内源序列）的mRNA水平而不降低一个或多个密切相关序列的mRNA水平。虽然miRNA可以针对特定靶序列被设计为特异性，但是本申请表明miRNA可以特异性沉默靶序列而不沉默与靶序列具有高序列同一性的紧密相关的序列。在某些实施方案中，可以用重组miRNA表达构建体抑制内源靶序列，而不沉默具有与靶序列密切相关的序列的目标重组多核苷酸。这些方法和组合物使用产生21-nt miRNA的重组miRNA表达构建体。还提供了转基因植物细胞，植物和掺入miRNA表达构建体的种子和包含感兴趣的多核苷酸的重组多核苷酸构建体。

76. 发明申请

WO2013055916A1 METHODS FOR IMPROVING SEED PRODUCTION IN MAIZE 审中-公开
标题翻译：改善玉米种子生产的方法
公开(公告)号：WO2013055916A1
公开(公告)日：2013-04-18
申请号：PCT/US2012/059747
申请日：2012-10-11
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： MIHURA, Eduardo , NELSON, Scott , SAAB, Imad , SAYERS, Adda , SCHWARTE, Aaron
IPC分类号： A01C1/00 , A01H5/10 , A01G7/00
CPC分类号： A01N59/00 , A01H5/10 , Y02A40/14
摘要： Defoliation of maize plants, based on percent seed moisture, has advantageous outcomes including greater number of seeds per pound; increased volume or proportion of saleable seed per field and per female acre; decreased discard of seed due to commercially undesirable size or shape; lower moisture content of seed at harvest; earlier harvest date; less fuel and time expended in drying seed for storage; improved performance in laboratory tests for germination at cold temperatures; improved seed treatment efficacy; improved emergence under stress in field conditions; improved plantability in mechanical systems; more uniform stand; fewer runt plants and improved grain yield.
摘要翻译：基于种子水分百分比，玉米植物脱叶具有有利的结果，包括每磅更多的种子数; 每场和每英亩的可销售种子的数量或比例增加; 由于商业上不合适的尺寸或形状而减少种子的丢弃; 收获时种子含水量较低; 收获日期早减少燃料和时间消耗在干燥种子储存; 改善在寒冷的温度下发芽实验室测试的性能; 提高种子处理效果; 改善现场条件下的压力出现; 改善机械系统的可植入性; 更统一的立场更少的矮秆植物和粮食产量提高。

77. 发明申请

WO2013055657A1 AUTOMATIC DETECTION OF OBJECT PIXELS FOR HYPERSPECTRAL ANALYSIS 审中-公开
标题翻译：自动检测用于高分辨分析的对象像素
公开(公告)号：WO2013055657A1
公开(公告)日：2013-04-18
申请号：PCT/US2012/059299
申请日：2012-10-09
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： JANNI, James
IPC分类号： G06K9/20
CPC分类号： G06K9/2018 , G06K2209/17
摘要： A method is provided for automatically discerning between object and non-object pixels in a hyperspectral image data cube. In particular embodiments, the object of the method is a plant, plant part, plant trait, plant phenotype, plant pot or a plant medium. The method comprises a first step of providing a partial least squares discriminant analysis (PLSDA) algorithm and a second step of applying the PLSDA algorithm to a hyperspectral image data cube to automatically determine which pixels contain the spectral properties of the object. The PLSDA algorithm of the method can be generated by establishing a training matrix, performing an eigenvector decomposition of the training matrix, experimentally determining a weighted linear combination of object signal-containing eigenvectors, calculating a regression vector using the weighted linear combination of signal-containing eigenvectors, generating a mask matrix and multiplying the mask matrix by the hyperspectral image data cube along two spatial dimensions.
摘要翻译：提供了一种用于在高光谱图像数据立方体中自动辨别对象和非对象像素之间的方法。在具体实施方案中，该方法的目的是植物，植物部分，植物性状，植物表型，植物花盆或植物培养基。该方法包括提供偏最小二乘判别分析（PLSDA）算法的第一步骤和将PLSDA算法应用于高光谱图像数据立方体以自动确定哪些像素包含对象的光谱特性的第二步骤。该方法的PLSDA算法可以通过建立训练矩阵，执行训练矩阵的特征向量分解，实验确定包含目标信号的特征向量的加权线性组合，使用加权的线性组合计算回归矢量生成掩模矩阵，并沿着两个空间维度将掩模矩阵乘以高光谱图像数据立方体。

78. 发明申请

WO2013019461A1 TERMINATOR SEQUENCE FOR GENE EXPRESSION IN PLANTS 审中-公开
标题翻译：用于植物中基因表达的终止子序列
公开(公告)号：WO2013019461A1
公开(公告)日：2013-02-07
申请号：PCT/US2012/047901
申请日：2012-07-23
申请人： PIONEER HI-BRED INTERNATIONAL , ABBITT, Shane E. , JUNG, Rudolf
发明人： ABBITT, Shane E. , JUNG, Rudolf
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8216
摘要： The present invention discloses polynucleotide sequences that can be used to regulate gene expression in plants. Terminator sequences from Sorghum bicolor that are functional in plants are disclosed.
摘要翻译：本发明公开了可用于调节植物中基因表达的多核苷酸序列。公开了在植物中功能的双粱高粱的终止子序列。

79. 发明申请

WO2012112411A1 ROOT-PREFERRED PROMOTER AND METHODS OF USE 审中-公开
标题翻译：根本优先促销者和使用方法
公开(公告)号：WO2012112411A1
公开(公告)日：2012-08-23
申请号：PCT/US2012/024802
申请日：2012-02-13
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , DIEHN, Scott , PETERSON-BURCH, Brooke
发明人： DIEHN, Scott , PETERSON-BURCH, Brooke
IPC分类号： C12N15/82 , C07K14/415 , A01H5/00
CPC分类号： C12N15/8227 , C07K14/415
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding Sorghum bicolor RCc3. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码高粱双色RCc3的基因的启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括用与本发明的启动子之一可操作地连接的核苷酸序列转化植物或植物细胞。

80. 发明申请

WO2012087903A2 DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING MATE-EFFLUX POLYPEPTIDES 审中-公开
标题翻译：干旱耐受植物及相关构建体和方法涉及基因编码的MATE-EFFLUX多肽
公开(公告)号：WO2012087903A2
公开(公告)日：2012-06-28
申请号：PCT/US2011/065779
申请日：2011-12-19
申请人： E. I. du Pont de Nemours and Company , PIONEER HI-BRED INTERNATIONAL, INC. , ALLEN, Stephen, M. , BRUGIERE, Norbert , KUMRIA, Ratna , LUCK, Stanley , MOHANTY, Amitabh , MULLEN, Jeffrey , RAJA, Rupa , SAKAI, Hajime , TINGEY, Scott, V. , WILLIAMS, Robert, Wayne
发明人： ALLEN, Stephen, M. , BRUGIERE, Norbert , KUMRIA, Ratna , LUCK, Stanley , MOHANTY, Amitabh , MULLEN, Jeffrey , RAJA, Rupa , SAKAI, Hajime , TINGEY, Scott, V. , WILLIAMS, Robert, Wayne
IPC分类号： C07K14/415
CPC分类号： C12N15/8271 , C07K14/415 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a MATE-efflux polypeptide.
摘要翻译：可用于赋予耐旱性的分离的多核苷酸和多肽和重组DNA构建体，包含这些重组DNA构建体的组合物（如植物或种子），以及利用这些重组DNA构建体的方法。重组DNA构建体包含可操作地连接至在植物中有功能的启动子的多核苷酸，其中所述多核苷酸编码MATE-外排多肽。

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