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71. 发明授权

US5869316A Biologically pure culture of aureobacterium barkeri KDO-37-2 失效
标题翻译：生物纯培养的巴氏杆菌KDO-37-2
公开(公告)号：US5869316A
公开(公告)日：1999-02-09
申请号：US767182
申请日：1996-12-16
申请人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
IPC分类号： C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , C12P19/02 , C12N1/20
CPC分类号： C12P19/02 , C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , Y10S435/822
摘要： Aureobacterium barkeri strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.
摘要翻译：公开了分离自巴斯德氏菌杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。另外，保护形式的KDO醛缩酶产物，例如，六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

72. 发明授权

US5795749A Use of 2-deoxyribose-5-phosphate aldolase to prepare 2-deoxyfucose, analogues and derivatives 失效
标题翻译：使用2-脱氧核糖-5-磷酸醛缩酶制备2-脱氧岩藻糖，类似物和衍生物
公开(公告)号：US5795749A
公开(公告)日：1998-08-18
申请号：US416999
申请日：1995-04-05
申请人： Chi-Huey Wong , Harrie J. M. Gijsen
发明人： Chi-Huey Wong , Harrie J. M. Gijsen
IPC分类号： C12P19/02 , C07H1/00 , C12P11/00 , C12P19/00
CPC分类号： C12P19/02
摘要： Processes using 2-deoxyribose-5-phosphate aldolase (DERA) are described for the preparation of 2-deoxyfucose and related compounds. In one embodiment, DERA is used to catalyze the condensation of acetaldehyde as donor and a 2(R)-hydroxy-3-(hydroxy or mercapto)-propionaldehyde derivative to form a 2-deoxysugar whose hydroxyls have the configuration of fucose. In another embodiment, DERA is used to catalyze the condensation of two moles of acetaldehyde as donor and one mole of a 2-substituted acetaldehyde acceptor to form a 2,4,6-trideoxyhexose via a 4-substituted-3-hydroxybutanal intermediate.
摘要翻译：描述了使用2-脱氧核糖-5-磷酸醛缩酶（DERA）的方法用于制备2-脱氧岩藻糖和相关化合物。在一个实施方案中，DERA用于催化作为供体的乙醛和2（R） - 羟基-3-（羟基或巯基） - 丙醛衍生物的缩合形成其羟基具有岩藻糖构型的2-脱氧糖。在另一个实施方案中，DERA用于催化2摩尔乙醛供体和1摩尔2-取代乙醛受体的缩合，以通过4-取代-3-羟基丁醛中间体形成2,4,6-三脱氧己糖。

73. 发明授权

US5599915A Sialyl Lewis X mimetics 失效
标题翻译：唾液酸Lewis X模拟物
公开(公告)号：US5599915A
公开(公告)日：1997-02-04
申请号：US407912
申请日：1995-03-21
申请人： Chi-Huey Wong , Tetsuya Kajimoto
发明人： Chi-Huey Wong , Tetsuya Kajimoto
IPC分类号： A61K38/00 , C07H7/02 , C07H15/04 , C07H15/22 , C07K9/00 , A61K31/70 , C08B37/00
CPC分类号： C07H15/22 , C07H15/04 , C07H7/02 , C07K9/001 , A61K38/00
摘要： Sialyl Lewis.sup.x mimetic compounds are disclosed that are free of sialyl groups and glycosidically-linked fucosyl groups. These compounds exhibit about the same inhibition of selectin-medicated cellular adhesion as does sialyl Lewis.sup.x itself.
摘要翻译：公开了没有唾液酸基团和糖苷键连接的岩藻糖基团的唾液酸Lewisx模拟化合物。这些化合物表现出与唾液酸Lewisx本身相当的选择素药物细胞粘附的抑制。

74. 发明授权

US5597906A Carbon linked glycosyl compounds 失效
标题翻译：碳链糖基化合物
公开(公告)号：US5597906A
公开(公告)日：1997-01-28
申请号：US33608
申请日：1993-03-18
申请人： Chi-Huey Wong , Hirosato Kondo
发明人： Chi-Huey Wong , Hirosato Kondo
IPC分类号： C07D309/10 , C07H15/00
CPC分类号： C07D309/10
摘要： Carbon linked glycosyl compounds are disclosed and synthesized. The synthesis employs a blocked carbohydrate donor and a blocked glycosyl acceptor. The blocked carbohydrate donor includes an acid labile phosphite leaving group attached to the anomeric carbon. The blocked glycosyl acceptor includes an unprotected carbon susceptible to electrophilic attack. The reaction is initiated by the addition of a Lewis acid so as to activate the acid labile phosphite leaving group on the carbohydrate donor. The substitution reaction may be conducted at -78.degree. C. in a solvent which favors the formation of carbon linked glycosylation products.
摘要翻译：公开并合成了碳链糖基化合物。该合成使用封闭的碳水化合物供体和封闭的糖基受体。封闭的碳水化合物供体包括与异头碳连接的酸不稳定的亚磷酸酯离去基团。封闭的糖基受体包括易受亲电发作的未受保护的碳。通过加入路易斯酸来引发反应，以活化碳水化合物供体上的酸不稳定亚磷酸酯离去基团。取代反应可以在-78℃下在有利于形成碳连接的糖基化产物的溶剂中进行。

75. 发明授权

US5576426A Aldolase catalyzed stereoselective synthesis of arabinohexulose, xyloheptulose, threohexulose and xylohexulose 失效
标题翻译：醛缩酶催化立体选择性合成阿拉伯己离酮糖，木瓜庚酮糖，苏洛木糖和己基己糖
公开(公告)号：US5576426A
公开(公告)日：1996-11-19
申请号：US303289
申请日：1994-09-08
申请人： Chi-Huey Wong , John R. Durrwachter , Richard L. Pederson
发明人： Chi-Huey Wong , John R. Durrwachter , Richard L. Pederson
IPC分类号： C07H5/04 , C07H7/02 , C07H11/00 , C07H13/04 , C12P19/26 , C12P41/00 , C07H5/06
CPC分类号： C07H13/04 , C07H11/00 , C07H5/04 , C07H7/02 , C12P19/26 , C12P41/00
摘要： The present invention relates to aldolase catalyzed stereo-selective synthesis of sugars and compositions of matter comprising arabinohexulose, xyloheptulose, threohexulose, and xylohexulose.
摘要翻译：本发明涉及醛缩酶催化的立体选择性合成糖和包含阿拉伯己离酮糖，木瓜庚酮糖，苏木素和木糖己糖的物质组合物。

76. 发明授权

US5565585A Azido diacetal propane derivatives 失效
标题翻译：叠氮二缩醛丙烷衍生物
公开(公告)号：US5565585A
公开(公告)日：1996-10-15
申请号：US225225
申请日：1994-04-08
申请人： Chi-Huey Wong , Richard L. Pederson , Yi-Fong Wang
发明人： Chi-Huey Wong , Richard L. Pederson , Yi-Fong Wang
IPC分类号： C07C45/89 , C07C247/04 , C07F9/02 , C07C211/00
CPC分类号： C07C247/04 , C07C45/89
摘要： A new and practical method for synthesizing heterocyclic polyhydroxylated alkaloids using enzymatic aldol condensation and catalytic intramolecular reductive amination is disclosed.
摘要翻译：公开了一种使用酶促醛缩醇缩合和催化分子内还原胺化合成杂环多羟基化生物碱的新型实用方法。

77. 发明授权

US5403726A Enzymatic process for producing a galactosyl.beta.1,3glycal disaccaride using .beta.-galactosidase 失效
标题翻译：使用β-半乳糖苷酶制备半乳糖基β1,3-二脱水酶的酶法
公开(公告)号：US5403726A
公开(公告)日：1995-04-04
申请号：US915465
申请日：1992-07-16
申请人： Chi-Huey Wong , Gary C. Look
发明人： Chi-Huey Wong , Gary C. Look
IPC分类号： C07H3/06 , C07H5/02 , C07H11/04 , C07H13/04 , C07H13/08 , C07H15/18 , C07H19/20 , C12N9/12 , C12P19/04 , C12P19/14 , C12P19/18 , C12P19/26 , C12P19/12 , C12N9/38
CPC分类号： C07H3/06 , C07H11/04 , C07H13/04 , C07H13/08 , C07H15/18 , C07H19/20 , C07H5/02 , C12N9/1241 , C12P19/04 , C12P19/14 , C12P19/18 , C12P19/26
摘要： An enzymatic process is disclosed for the preparation of galactosyl.beta.1,3glycal disaccharides such as Gal.beta.1,3Glucal, an intermediate useful in Le.sup.a preparation and an inhibitor of .beta.-galactosidase. The process utilizes .beta.-galactosidase, an enzyme usually used for bond breaking, to form a bond between a galactoside and a glucal such as glycal, a 6-O--C.sub.1 -C.sub.6 acylglucal or 6-O--C.sub.1 -C.sub.6 acetylgalactal.
摘要翻译：公开了用于制备半乳糖基β1,3-二糖二糖的酶促方法，例如Galβ1,3Glucal，可用于Lea制剂的中间体和β-半乳糖苷酶的抑制剂。该方法使用通常用于断裂的酶的β-半乳糖苷酶在半乳糖苷和葡萄糖如甘氨酸，6-O-C1-C6酰基葡萄糖或6-O-C1-C6乙酰半乳糖之间形成键。

78. 发明授权

US5358859A KDO Aldolase and condensation reactions employed therewith 失效
标题翻译： KDO醛缩酶和与其一起使用的缩合反应
公开(公告)号：US5358859A
公开(公告)日：1994-10-25
申请号：US993140
申请日：1992-12-18
申请人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
IPC分类号： C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , C12P19/02
CPC分类号： C12P19/02 , C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , Y10S435/822
摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The DKDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.
摘要翻译：公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）KDO醛缩酶（EC 4.1.2.23）。进一步公开了DKDO醛缩酶对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。另外，保护形式的KDO醛缩酶产物，例如，六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

79. 发明授权

US5352591A Aldolase enzyme process for making 5-azido-5-deoxy-hexulose-1-phosphate 失效
标题翻译：制备5-叠氮基-5-脱氧 - 己糖-1-磷酸的醛缩酶酶法
公开(公告)号：US5352591A
公开(公告)日：1994-10-04
申请号：US93782
申请日：1993-07-19
申请人： Chi-Huey Wong , Kun-Chin Liu
发明人： Chi-Huey Wong , Kun-Chin Liu
IPC分类号： C07D207/12 , C07D207/46 , C07H7/02 , C07H11/00 , C12P17/10 , C07D207/30 , C12P19/02
CPC分类号： C07D207/46 , C07D207/12 , C07H11/00 , C07H7/02
摘要： 2-Methyl-5-hydroxymethyl-and 2,5-dimethyl-3,4-dihydroxypyrrolidines, their processes of preparation and use are disclosed. 5-Azido-5-deoxy-hexulose-1-phosphate compounds and processes of making the same are also disclosed.
摘要翻译：公开了2-甲基-5-羟甲基 - 和2,5-二甲基-3,4-二羟基吡咯烷的制备方法和用途。还公开了5-叠氮基-5-脱氧 - 己糖-1-磷酸酯化合物及其制备方法。

80. 发明授权

US5225339A Lactobacillus kefir alcohol dehydrogenase 失效
标题翻译：乳酸杆菌酒精脱氢酶
公开(公告)号：US5225339A
公开(公告)日：1993-07-06
申请号：US841701
申请日：1992-02-26
申请人： Chi-Huey Wong , Curt W. Bradshaw
发明人： Chi-Huey Wong , Curt W. Bradshaw
IPC分类号： C07F7/08 , C12N9/04 , C12P7/02 , C12P7/22 , C12P7/26 , C12P9/00 , C12P17/00 , C12P41/00
CPC分类号： C12N9/0006 , C07F7/0818 , C07F7/083 , C12P17/00 , C12P41/002 , C12P7/02 , C12P7/22 , C12P7/26 , C12P9/00 , Y10S435/853
摘要： The present invention discloses the preparation of R-configured trialkylsilylethynyl .alpha.-alcohols from similarly substituted .alpha.-ketones, and vice versa, catalyzed by the alcohol dehydrogenase of Lactobacillus kefir, ATCC No. 35411. Also disclosed is the acceptance or transfer of a hydride ion from or to the pro-R face of NADPH or NADP, respectively, as catalyzed by that enzyme.
摘要翻译：本发明公开了由来自类似取代的α-酮的R-配位的三烷基甲硅烷基乙炔基α-醇的制备，反之亦然，由Kactir Lactobacillus kefir的醇脱氢酶，ATCC No.35411催化。还公开了氢化物离子的接受或转移分别从NADPH或NADP的pro-R表面催化，由该酶催化。

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