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61. 发明申请

US20040209264A1 New essential downstream component of the wingless signalling pathway 审中-公开
标题翻译：无翼信号通路的新要素下游成分
公开(公告)号：US20040209264A1
公开(公告)日：2004-10-21
申请号：US10472385
申请日：2004-04-27
发明人： Thomas Kramps , Konrad Basler
IPC分类号： C12Q001/68 , C07H021/04 , C12N005/06 , C07K014/435
CPC分类号： G01N33/57407 , C07K14/43581 , C07K14/4702 , C07K2319/00 , G01N33/5082 , G01N2520/00
摘要： The present invention relates to a new essential downstream component of the wingless signaling pathway. In particular, the invention relates to nucleotide sequences of the Drosophila melanogaster daughter of legless (doll) gene, of its encoded proteins, as well as derivatives, fragments and analogues thereof. The invention includes vertebrate and invertebrate homologues of the Doll protein, comprising proteins that contain a stretch of amino acids with similarity to the Drosophila Doll gene. Methods for producing the Doll protein, derivatives and analogs, e.g. by recombinant means, and antibodies to Doll are provided by the present invention as well. The invention also relates to methods for performing high throughput screening assays for compounds modulating Doll function in the Wnt pathway.
摘要翻译：本发明涉及无翼信令通路的新的基本下游部件。特别地，本发明涉及无基因（洋娃娃）基因，其编码的蛋白质以及其衍生物，片段和类似物的黑腹果蝇的雌性核苷酸序列。本发明包括Doll蛋白的脊椎动物和无脊椎动物同系物，其包含含有与果蝇娃娃基因相似的一段氨基酸的蛋白质。生产Doll蛋白，衍生物和类似物的方法，例如通过重组方法，本发明也提供了对Doll的抗体。本发明还涉及用于在Wnt途径中调节Doll功能的化合物进行高通量筛选测定的方法。

62. 发明申请

US20030211041A1 Water monitoring system using bivalve mollusks 失效
标题翻译：使用双壳软体动物的水监测系统
公开(公告)号：US20030211041A1
公开(公告)日：2003-11-13
申请号：US10343660
申请日：2003-02-03
发明人： Jason Ezratty , Robert Morgan , David Eddy , Donald Morgan
IPC分类号： A61K049/00 , G06F019/00 , G01N033/48 , G01N033/50
CPC分类号： G01N33/186 , G01N2520/00
摘要： A toxicant detection system comprises at least one watertight chamber containing a mollusk. Water to be screened is introduced into the chamber. The mollusk is preferably supported on a mounting structure which facilitates quick mounting of the mollusk in the device, comprising a releasable element affixed to the shell of the mollusk, e.g., a bolt which is screwed into a nut affixed to the mollusk's shell. A sensing apparatus is provided which includes a movable member supported in the chamber so that it engages the shell of the mollusk, and moves when the shell opens and closes. The sensing apparatus detects the position of the mollusk shell based on the position of the movable member. The sensing apparatus preferably includes a Hall effect transducer which co-acts with a magnet carried on the movable member. The system allows for accurate readings of the small movements of the mollusk shell which aid in detecting toxicants, and also provides for installation of mollusks of varying sizes, without the need to move components of the standard configuration of the chamber. Methods and systems for detecting or identifying a toxicant in water being screened are also described.
摘要翻译：毒物检测系统包括至少一个包含软体动物的水密室。要被筛选的水被引入室内。软体动物优选地支撑在便于将软体动物快速安装在装置中的安装结构上，该安装结构包括固定在软体动物的壳体上的可释放元件，例如拧入固定在软体动物壳上的螺母的螺栓。提供了一种感测装置，其包括支撑在室中的可动构件，使得其接合软体动物的壳体，并且当壳体打开和关闭时移动。感测装置基于可动构件的位置来检测软体动物壳的位置。感测装置优选地包括霍尔效应传感器，其与携带在可动构件上的磁体共同作用。该系统允许准确读取软体动物壳的小的运动，其有助于检测毒物，并且还提供安装不同尺寸的软体动物，而不需要移动腔室的标准构造的部件。还描述了用于检测或鉴定被筛选的水中的毒物的方法和系统。

63. 发明申请

US20030118984A1 Tissue-based water quality biosensors for detecting chemical warfare agents 失效
公开(公告)号：US20030118984A1
公开(公告)日：2003-06-26
申请号：US10339197
申请日：2003-01-09
发明人： Elias Greenbaum , Charlene A. Sanders
IPC分类号： C12Q001/00 , G06F019/00 , G01N033/48 , G01N033/50 , C12Q001/04
CPC分类号： G01N33/5097 , C12Q1/02 , G01N2021/635 , G01N2520/00
摘要： A method of testing water to detect the presence of at least one chemical or biological warfare agent includes the steps of: a. exposing water to a measuring means for measuring photosynthetic activity of free-living indigenous photosynthetic organisms; b. measuring photosynthetic activity of indigenous photosynthetic organisms occurring naturally in the water to detect the presence of at least one chemical or biological warfare agent in the water.

64. 发明申请

US20030003491A1 Method for detecting or quantifying environmental hormones 审中-公开
标题翻译：检测或定量环境激素的方法
公开(公告)号：US20030003491A1
公开(公告)日：2003-01-02
申请号：US10169938
申请日：2002-07-11
发明人： Takashi Minegishi , Kaoru Miyamoto
IPC分类号： C12Q001/00 , C12Q001/68 , G01N033/567
CPC分类号： G01N33/5308 , G01N33/5008 , G01N33/5014 , G01N33/5023 , G01N33/5044 , G01N33/76 , G01N2333/59 , G01N2333/726 , G01N2430/30 , G01N2430/40 , G01N2500/00 , G01N2520/00
摘要： The present invention relates to a method for the detection or determination of environmental hormones, in particular, dioxins in a sample using granulosa cells, etc. In the present invention, the change in the amount of an expressed luteinizing hormone receptor induced by follicle-stimulating hormone, etc. in granulosa cells is employed as an indicator for the detection or determination of dioxins. The present invention also relates to a method for contraception comprising inhibiting the expression of luteinizing hormone receptor induced by follicle-stimulating hormone in granulosa cells, and to a contraceptive agent comprising, as an active ingredient, a substance which inhibits the expression of luteinizing hormone receptor.
摘要翻译：本发明涉及一种检测或测定环境激素，特别是使用颗粒细胞等的样品中的二恶英的方法。在本发明中，由促卵泡激素诱导的表达黄体生成素受体的量的变化使用颗粒细胞中的激素等作为二恶英的检测或测定的指标。本发明还涉及一种避孕方法，包括抑制颗粒细胞中由促卵泡激素诱导的促黄体激素受体的表达，以及包含抑制黄体生成激素受体表达的物质作为活性成分的避孕药。

65. 发明授权

US06436698B2 Apparatus for automatic measuring of water toxicity 失效
标题翻译：用于自动测量水毒性的装置
公开(公告)号：US06436698B2
公开(公告)日：2002-08-20
申请号：US09761654
申请日：2001-01-18
申请人： Han Oh Park , Hanee Park , Il Kyu Choi
发明人： Han Oh Park , Hanee Park , Il Kyu Choi
IPC分类号： C12M136
CPC分类号： C12Q1/02 , G01N21/763 , G01N33/1826 , G01N33/1866 , G01N2520/00 , Y02A20/206
摘要： The present invention relates to an apparatus for automatic measuring of water toxicity. More specifically, the present invention provides an apparatus for automatic and continuous measuring of water toxicity and/or contamination using luminescent microorganisms living in freshwater.
摘要翻译：本发明涉及一种用于自动测量水毒性的装置。更具体地说，本发明提供一种用于利用居住在淡水中的发光微生物自动连续地测量水毒性和/或污染的装置。

66. 发明授权

US06238928B1 Analytical process for testing mixtures for toxic constituents 失效
标题翻译：用于测试有毒成分混合物的分析方法
公开(公告)号：US06238928B1
公开(公告)日：2001-05-29
申请号：US08116382
申请日：1993-09-02
申请人： Claus Weisemann , Wolfgang Kreiss , Hans-Georg Rast , Günther Eberz
发明人： Claus Weisemann , Wolfgang Kreiss , Hans-Georg Rast , Günther Eberz
IPC分类号： G01N3090
CPC分类号： C12Q1/025 , G01N2520/00 , Y10T436/255
摘要： The mixture to be tested for toxic components is first separated into fractions by chromatography. The separated fractions are then contacted with luminescent microorganisms in the chromatographic system itself and the toxicity of the fractions is determined by measurement of the bioluminescence. The chromatographic separation may be carried out either by thin layer chromatography or by column liquid chromatography.
摘要翻译：待测试有毒成分的混合物首先通过色谱法分离成级分。然后将分离的级分与色谱系统中的发光微生物本身接触，并通过测量生物发光来测定级分的毒性。色谱分离可以通过薄层色谱法或通过柱色谱法进行。

67. 发明授权

US09921211B2 Methods for detecting and monitoring endocrine disrupting chemicals (EDCs) 有权
公开(公告)号：US09921211B2
公开(公告)日：2018-03-20
申请号：US14693511
申请日：2015-04-22
申请人： THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services
发明人： Gordon L. Hager , Diana A. Stavreva
IPC分类号： G01N33/53 , G01N33/50 , G01N21/64 , C07K14/435 , C07K14/705 , G01N33/68 , G01N33/74
CPC分类号： G01N33/5035 , C07K14/435 , C07K14/705 , C07K2319/60 , G01N21/6486 , G01N33/5014 , G01N33/68 , G01N33/743 , G01N2520/00
摘要： Described herein are compositions, methods, a system, and kits for detection of endocrine disruptor chemicals (EDCs) in samples, such as samples of water including but not limited to waste water treatment plant effluent, using a live-cell fluorescence-based nuclear translocation reporter system. Upon binding of a ligand to a fluorescent-labeled reporter protein, the protein (and therefore the fluorescence) is translocated in a ligand level-dependent manner from the cytoplasm to the nucleus of live mammalian cells; this translocation is detectable as diffuse (cytoplasmic) fluorescence converting to localized, brightly fluorescent nuclei. The described kits can be used to reliably detect very low levels of EDC contamination, including in high throughput analysis systems as described.

68. 发明申请

US20150219628A1 METHODS FOR DETECTING AND MONITORING ENDOCRINE DISRUPTING CHEMICALS (EDCs) 有权
标题翻译：检测和监测内毒素化学物质（EDCs）的方法
公开(公告)号：US20150219628A1
公开(公告)日：2015-08-06
申请号：US14693511
申请日：2015-04-22
申请人： THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human
发明人： Gordon L. Hager , Diana A. Stavreva
IPC分类号： G01N33/50 , G01N33/74
CPC分类号： G01N33/5035 , C07K14/435 , C07K14/705 , C07K2319/60 , G01N21/6486 , G01N33/5014 , G01N33/68 , G01N33/743 , G01N2520/00
摘要： Described herein are compositions, methods, a system, and kits for detection of endocrine disruptor chemicals (EDCs) in samples, such as samples of water including but not limited to waste water treatment plant effluent, using a live-cell fluorescence-based nuclear translocation reporter system. Upon binding of a ligand to a fluorescent-labeled reporter protein, the protein (and therefore the fluorescence) is translocated in a ligand level-dependent manner from the cytoplasm to the nucleus of live mammalian cells; this translocation is detectable as diffuse (cytoplasmic) fluorescence converting to localized, brightly fluorescent nuclei. The described kits can be used to reliably detect very low levels of EDC contamination, including in high throughput analysis systems as described.
摘要翻译：本文描述的是用于检测样品中的内分泌干扰物质化学品（EDC）的组合物，方法，系统和试剂盒，例如水样品，包括但不限于废水处理厂废水，使用基于活细胞荧光的核易位记者系统。在将配体结合到荧光标记的报道蛋白上时，蛋白质（因此荧光）以配体水平依赖的方式从细胞质转移到活哺乳动物细胞的细胞核; 这种易位可检测为扩散（细胞质）荧光转化为局部的，明亮的荧光核。所描述的套件可用于可靠地检测非常低水平的EDC污染，包括在所述的高通量分析系统中。

69. 发明申请

US20140227737A1 COLIFORM DETECTION PROCESS AND KIT FOR USE THEREIN 审中-公开
标题翻译：检测过程及其使用的工具包
公开(公告)号：US20140227737A1
公开(公告)日：2014-08-14
申请号：US14254236
申请日：2014-04-16
申请人： 3M INNOVATIVE PROPERTIES COMPANY
发明人： Manjiri T. Kshirsagar
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/24 , G01N33/56916 , G01N2520/00
摘要： A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.
摘要翻译：一种方法包括（a）提供（1）至少一种怀疑含有至少一种大肠杆菌菌株的样品，（2）至少一种包含至少一种水合或可水合的培养基的培养装置，和（3）至少一种当培养基水合时，当与培养装置中的培养基接触时基本上是光学透明的颗粒浓缩剂; （b）使颗粒浓缩剂与样品接触，使得至少一部分大肠杆菌菌株与颗粒物浓缩剂结合形成大肠菌群结合的颗粒浓缩剂; （c）使大肠杆菌结合的颗粒浓缩剂与培养基接触; （d）将包含大肠杆菌结合的颗粒浓缩剂的培养装置与培养基接触培养，培养基水合; 和（e）光学检测大肠菌群的存在，而不从大颗粒浓缩剂中分离大肠杆菌菌株。

70. 发明授权

US08741595B2 Coliform detection process and kit for use therein 有权
标题翻译：大肠杆菌检测过程及其中使用的试剂盒
公开(公告)号：US08741595B2
公开(公告)日：2014-06-03
申请号：US13142455
申请日：2009-12-29
申请人： Manjiri T. Kshirsagar
发明人： Manjiri T. Kshirsagar
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/24 , G01N33/56916 , G01N2520/00
摘要： A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.
摘要翻译：一种方法包括（a）提供（1）至少一种怀疑含有至少一种大肠杆菌菌株的样品，（2）至少一种包含至少一种水合或可水合的培养基的培养装置，和（3）至少一种当培养基水合时，当与培养装置中的培养基接触时基本上是光学透明的颗粒浓缩剂; （b）使颗粒浓缩剂与样品接触，使得至少一部分大肠杆菌菌株与颗粒物浓缩剂结合形成大肠菌群结合的颗粒浓缩剂; （c）使大肠杆菌结合的颗粒浓缩剂与培养基接触; （d）将包含大肠杆菌结合的颗粒浓缩剂的培养装置与培养基接触培养，培养基水合; 和（e）光学检测大肠菌群的存在，而不从大颗粒浓缩剂中分离大肠杆菌菌株。

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