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61. 发明申请

US20180080003A1 INTERCELLULAR SEPARATION METHOD FOR CULTURED CELLS 审中-公开
公开(公告)号：US20180080003A1
公开(公告)日：2018-03-22
申请号：US15559016
申请日：2016-04-05
申请人： MEDIKAN INC.
发明人： HEE YOUNG LEE
IPC分类号： C12N1/02 , C12N5/00 , C12M1/00
CPC分类号： C12N1/02 , C12M1/12 , C12M47/02 , C12M47/04 , C12N5/00 , C12N2509/10 , C12Q1/24
摘要： The present invention relates to a method of separating cultured cells, which is used in a configuration including: a first accommodation space; a cell suspension which is present in a liquid in the first accommodation space in a state in which a plurality of cells is connected to one another; a filter container which is hermetically connected to the first accommodation space and mounted with a cell dividing member having one or more through holes having an average diameter of 5 μm to 300 μm; and a second accommodation space which is hermetically connected to the filter container, in which the cell suspension flows from the first accommodation space to the second accommodation space through the filter, such that the connection between the cells is passively and mechanically separated while the cells collide with a fixed resistance portion of the filter.

62. 发明申请

US20180021737A1 FILTRATION FILTER 审中-公开
公开(公告)号：US20180021737A1
公开(公告)日：2018-01-25
申请号：US15719942
申请日：2017-09-29
申请人： Murata Manufacturing Co., Ltd.
发明人： MASARU BANJU , Takashi Kondo , Junko Watanabe
IPC分类号： B01D71/02 , B01D69/02 , B01D69/10
CPC分类号： B01D71/022 , B01D63/087 , B01D69/02 , B01D69/10 , B01D2313/025 , B01D2313/20 , B01D2315/10 , B01D2325/04 , C12M1/12 , C12M29/18 , C12M33/14 , C12M47/02
摘要： A filtration filter is suitable for performing cross-flow filtration by using a metallic porous membrane. A metallic porous membrane has a membrane portion for filtering filtration objects contained in a fluid and a held portion provided at its outer periphery. A first frame member and a second frame member hold the held portion of the metallic porous membrane there between. The held portion has a bent portion bent to a second principal surface side opposing a first principal surface of the membrane portion. The first frame member is in contact with the held portion at the first principal surface side of the metallic porous membrane. The second frame member is disposed at an inner side portion of the first frame member and is in contact with the held portion at the second principal surface side of the metallic porous membrane.

63. 发明申请

US20170322210A1 In Vitro Oral Biofilm Models of Interdental Spaces and Uses 审中-公开
公开(公告)号：US20170322210A1
公开(公告)日：2017-11-09
申请号：US15523792
申请日：2014-11-11
申请人： Colgate-Palmolive Company
发明人： Hans Stettler , Amy Russo RUSSO
IPC分类号： G01N33/569 , C12M1/12 , C12Q1/18
CPC分类号： G01N33/56955 , C12M1/12 , C12Q1/18
摘要： Disclosed is an in vitro oral biofilm model of interdental spaces including: a support, and a plurality of substratum pairs attached to the support, wherein an oral biofilm is capable of forming on the substratum pairs, wherein the substratum pairs each include a first member and a second member, and wherein the first member and the second member of each substratum pair are arranged to form a space interval between the first member and second member. Methods for assessing the formation of oral biofilms using the in vitro oral biofilm model and methods for testing agents, such as oral compositions, on biofilm reduction using the present model are also provided.

64. 发明授权

US09428724B2 Bioreactor with feed and harvest flow through filter assembly 有权
标题翻译：具有进料和收获的生物反应器流过过滤器组件
公开(公告)号：US09428724B2
公开(公告)日：2016-08-30
申请号：US13984937
申请日：2012-02-22
申请人： Patric Fricking
发明人： Patric Fricking
IPC分类号： C12M1/12 , C12M1/00 , C12M3/06 , C12M1/26
CPC分类号： C12M33/14 , C12M1/12 , C12M3/06 , C12M3/067 , C12M21/00 , C12M27/16 , C12M29/04 , C12M29/10
摘要： The present invention relates to a bioreactor and a method for operating a bioreactor which comprises a chamber (100) capable of receiving a liquid media, and a filter assembly (200) comprising a perfusion filter (210; 212; 214). The filter assembly (200) is disposed in the chamber (100) and the filter assembly (200) is free to move within the chamber (100). The filter assembly (200) comprises means for coupling a harvesting flow through said filter (210; 212; 214) and means for coupling a feed flow through said filter in an opposite direction compared to the harvesting flow. Through this arrangement, the clogging prevention of the perfusion filter can be improved.
摘要翻译：本发明涉及一种生物反应器和一种用于操作生物反应器的方法，该生物反应器包括能够接收液体介质的室（100）和包括灌注过滤器（210; 212; 214）的过滤器组件（200）。过滤器组件（200）设置在室（100）中，并且过滤器组件（200）在室（100）内自由移动。过滤器组件（200）包括用于将通过所述过滤器（210; 212; 214）的收集流耦合的装置和用于将进料流与收获流相反的方向与相反方向耦合通过所述过滤器的装置。通过这种布置，可以提高灌注过滤器的堵塞防止。

65. 发明申请

US20120168390A1 Installation For Treating A Biological Liquid 有权
标题翻译：安装用于治疗生物液体
公开(公告)号：US20120168390A1
公开(公告)日：2012-07-05
申请号：US13420906
申请日：2012-03-15
申请人： Jean-Luc Beulay , Virginie Buisson , Sebastien Cirou , Cecile Delbos , Marc Hlavacek , Frans Mels , Rene Reinbigler , Jean-Louis Weissenbach
发明人： Jean-Luc Beulay , Virginie Buisson , Sebastien Cirou , Cecile Delbos , Marc Hlavacek , Frans Mels , Rene Reinbigler , Jean-Louis Weissenbach
IPC分类号： B01D37/00 , B01D37/04
CPC分类号： B01D36/00 , C12M1/12 , C12M47/12 , Y10T436/25375 , Y10T436/2575
摘要： Installation for treating a biological liquid, including a circulation pump (5), a filter element (9), a container for collecting treated liquid, a first circuit section connecting a source of said biological liquid to an inlet orifice of said filter element (9), including a circuit element adapted to cooperate with said circulation pump (5); a second circuit section, connecting an outlet orifice of said filter element (9) to said treated liquid collecting container; characterized in that said treated liquid collecting container, said first circuit section and said second circuit section are disposable and said installation further includes a first cart (1) carrying said pump (5) and a second cart (2) including a housing (14) for said collecting container, separable from the first cart (1) and adapted to be at least partly nested in said first cart (1).
摘要翻译：用于处理包括循环泵（5），过滤元件（9），用于收集处理液体的容器的生物液体的安装，将所述生物液体源连接到所述过滤元件（9）的入口孔的第一回路段），包括适于与所述循环泵（5）配合的电路元件; 第二电路部分，将所述过滤元件（9）的出口孔连接到所述处理过的液体收集容器; 其特征在于，所述处理过的液体收集容器，所述第一回路部分和所述第二回路部分是一次性的，并且所述装置还包括承载所述泵（5）的第一推车（1）和包括壳体（14）的第二推车（2）用于所述收集容器，所述收集容器可与所述第一推车（1）分离并且适于至少部分地嵌套在所述第一推车（1）中。

66. 发明申请

US20110189725A1 Microbiological Analysis Assembly And Method 有权
标题翻译：微生物分析装配与方法
公开(公告)号：US20110189725A1
公开(公告)日：2011-08-04
申请号：US13088632
申请日：2011-04-18
申请人： Stephane Olivier , Mathieu Arrault
发明人： Stephane Olivier , Mathieu Arrault
IPC分类号： C12Q1/02
CPC分类号： C12M29/04 , C12M1/12 , C12M23/26 , C12M23/32 , C12M23/42 , C12M25/14 , C12M33/14
摘要： The assembly comprises a filtration unit (1) and a gel growth medium cassette (80), the unit (1) and the cassette (80) being adapted to cooperate in order to occupy a nested position in which a membrane (3) covers the said gel growth medium (88), the said unit (1) and the said cassette (80) each being equipped with an intermediate stop (26, 100), it being possible for at least one of the two stops (100) to move clear so as to allow the said unit (1) to move from an intermediate position, in which the said membrane is spaced away from the said gel growth medium, into the said nested position.The method comprises the steps of selecting such an assembly, of engaging the said unit (1) on the said cassette (80) until the intermediate stop (26) of the said unit (1) comes into contact with the intermediate stop (100) of the said cassette (80), and the step of moving clear the said stop (100) able to move clear so as to allow the said unit (1) to move from the said intermediate position into the said nested position.
摘要翻译：组合件包括过滤单元（1）和凝胶生长培养基盒（80），单元（1）和盒（80）适于协作以便占据其中膜（3）覆盖的嵌套位置所述凝胶生长培养基（88），所述单元（1）和所述盒（80）各自配备有中间停止件（26,100），所述两个止挡件（100）中的至少一个能够移动透明以允许所述单元（1）从所述膜与所述凝胶生长介质间隔开的中间位置移动到所述嵌套位置。该方法包括以下步骤：选择这样的组件，使所述单元（1）与所述盒（80）接合，直到所述单元（1）的中间挡块（26）与中间止挡（100）接触，，并且移动所述止动件（100）的移动能够移动清除以允许所述单元（1）从所述中间位置移动到所述嵌套位置的步骤。

67. 发明授权

US07507579B2 Apparatus and methods for simultaneous operation of miniaturized reactors 失效
标题翻译：用于小型化反应堆同时操作的装置和方法
公开(公告)号：US07507579B2
公开(公告)日：2009-03-24
申请号：US10816046
申请日：2004-04-01
申请人： Paolo Boccazzi , Angela Y. Chen , Klavs F. Jensen , Nicolas Szita , Andrea Zanzotto , Zhiyu Zhang
发明人： Paolo Boccazzi , Angela Y. Chen , Klavs F. Jensen , Nicolas Szita , Andrea Zanzotto , Zhiyu Zhang
IPC分类号： C12M1/12 , C12M1/34 , C12M3/00 , C12M1/00 , G06K9/00
CPC分类号： C12M1/12 , B01F13/0059 , B01F13/0827 , B01J2219/00283 , B01J2219/00317 , B01J2219/00479 , B01J2219/00481 , B01J2219/00484 , B01J2219/00495 , B01J2219/00585 , B01J2219/00599 , B01J2219/00691 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/502723 , B01L9/527 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/0877 , B01L2300/10 , B01L2400/0466 , B01L2400/0487 , C12M23/16 , C12M35/08 , C12M41/48 , G01N2035/00326 , Y10S435/808 , Y10S435/809
摘要： The present invention provides a variety of microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. The invention further provides a chamber to contain the microfermentors and microfermentor arrays and to provide environmental control. Certain of the microfermentors include a second chamber that may be used, e.g., to provide oxygen, nutrients, pH control, etc., to the culture vessel and/or to remove metabolites, etc. Various methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided. The invention provides microreactors having a variety of different designs, some of which incorporate active stirring and/or have the capability to operate in batch or fed-batch mode. The invention further provides an apparatus and methods for simultaneous operation of a plurality of microreactors, with monitoring of the individual microreactors during a run. The invention further provides methods of performing gene expression analysis on cells cultured in microreactors.
摘要翻译：本发明提供用于培养细胞的各种微量生物反应器（微发酵器）和微生物生物反应器阵列。微发酵器包括用于培养细胞的容器和用于以足以支持细胞生长（例如细菌细胞生长）的浓度向容器内部提供氧气的装置。根据实施方案，微发酵罐容器可以具有小于约1ml的各种内部容积。微发酵器可以包括曝气膜和任选的各种感测装置。本发明进一步提供了容纳微发射体和微发光体阵列并提供环境控制的腔室。某些微发酵器包括第二室，其可以用于例如向培养容器提供氧气，营养物质，pH控制等，和/或去除代谢物等。使用微发酵器的各种方法，例如，选择最佳细胞株或生物过程参数。本发明提供具有各种不同设计的微反应器，其中一些具有主动搅拌和/或具有以分批或分批模式操作的能力。本发明还提供了一种用于同时操作多个微反应器的装置和方法，其中在运行期间监测各个微反应器。本发明还提供了对在微反应器中培养的细胞进行基因表达分析的方法。

68. 发明授权

US11789005B2 Device and method for determining degradation rate of biodegradable polymers in soil 有权
公开(公告)号：US11789005B2
公开(公告)日：2023-10-17
申请号：US17581918
申请日：2022-01-22
申请人： NORTH UNIVERSITY OF CHINA , SHANXI ZHONGBEI NEW MATERIAL TECHNOLOGY CO., LTD.
发明人： Yaqing Liu , Taian Chen , Yang Xiang , Guizhe Zhao
IPC分类号： G01N33/44 , G01N1/24 , G01N1/38 , C12M1/00 , C12M1/02 , C12M1/12 , C12M1/04
CPC分类号： G01N33/442 , C12M1/00 , C12M1/02 , C12M1/04 , C12M1/12 , G01N1/24 , G01N1/38
摘要： A device for determining degradation rate of biodegradable polymers in soil, including a micro compressed air pump, a first CO2 absorption vessel filled with dry soda lime or an aqueous strong alkali solution, a CO2 indicator vessel filled with a barium hydroxide solution, a hollow leaching device, a second CO2 absorption vessel and a third CO2 absorption vessel connected in sequence through connecting pipes. A top of the hollow leaching device is provided with an end cover. An external water distributor filled with a leaching solution and a mechanical stirring device is arranged above the end cover. The external water distributor is in communication with an inner cavity of the hollow leaching device through a pipeline. A stirring shaft of the mechanical stirring device extends into the inner cavity. A stirring shaft is provided with a stirring paddle.

69. 发明申请

US20190249126A1 INVERTED CULTURE PLATE SYSTEM FOR CELLULAR CO-CULTURE 审中-公开
公开(公告)号：US20190249126A1
公开(公告)日：2019-08-15
申请号：US16315753
申请日：2017-07-07
申请人： University of Maryland, Baltimore
发明人： Bryan Mackowiak , Hongbing Wang , Linhao Li
IPC分类号： C12M1/32 , C12M1/00 , C12N5/00
CPC分类号： C12M23/12 , B01L3/50853 , B01L2300/047 , B01L2300/0829 , B01L2300/0858 , B01L2300/163 , C12M1/12 , C12M3/00 , C12M3/06 , C12M23/34 , C12M25/04 , C12N5/0018
摘要： The present invention provides improved devices for co-culture of cells. The devices include inserts having invertible wells that can be lowered into a well of any standard cell culture plate. A first population of cells can be cultured in the invertible wells of the inserts and a second population of cells can be cultured in the wells of a cell culture plate. Once the first population of cells attach to the invertible wells, the inserts are flipped over and placed into the wells of the cell culture plate to co-culture with the second population of cells.

70. 发明申请

US20180346956A1 DEVICE FOR DETERMINING LIVE/DEAD BACTERIAL STATE AND METHOD FOR DETERMINING LIVE/DEAD BACTERIAL STATE USING THE DEVICE 审中-公开
公开(公告)号：US20180346956A1
公开(公告)日：2018-12-06
申请号：US15778877
申请日：2016-12-26
申请人： NIHON RIKAGAKU KAIHATSU LLC.
发明人： Toshikazu MORIWAKI , Hideki MORIWAKI
IPC分类号： C12Q1/06
CPC分类号： C12M1/12 , C12M1/34 , C12Q1/02
摘要： Provided is a device for determining the live/dead bacterial state, with which it is possible to ascertain the accurate state of live bacteria/dead bacteria via a captured image in a relatively easy operation with which ascertaining the state of a bacterial cell is more accurate than conventional methods. This device for determining the live/dead bacterial state comprises: a case body in which a measurement mechanism is housed; an opening/closing lid body that allows a fungus base insertion port formed in the case body to be opened and closed; and the measurement mechanism, which is housed in the case body and is configured to measure the number of bacteria. The measurement mechanism comprises: a fungus base holding mechanism that inserts and fixes a fungus base on which a bacterial cell collected from a specimen is placed; an excitation light irradiating mechanism configured so as to be capable of focusing and irradiating an excitation light toward the bacterial cell on the fungus base; an imaging camera disposed above the bacterial cell on the fungus base; and an XY-axes adjustment mechanism that minutely adjusts and moves, in the XY-axes, an XY stage supporting the fungus base holding mechanism and comprising two separately moving layers.

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