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    • 61. 发明申请
    • Plants Having Improved Growth Characteristics and Method for Making the Same
    • 具有改善生长特性的植物和制作相同的方法
    • US20080250534A1
    • 2008-10-09
    • US11660395
    • 2005-08-16
    • Valerie FrankardChristophe ReuzeauAna I. Sanz Molinero
    • Valerie FrankardChristophe ReuzeauAna I. Sanz Molinero
    • C12N15/11A01H5/00
    • C12N15/8262C07K14/415C12N15/8261Y02A40/146
    • The present invention concerns a method for improving the growth characteristics of plants by increasing activity in a plant of an RNA-binding protein or a homologue thereof, wherein said RNA-binding protein or homologue thereof is either: (i) a polypeptide having RNA-binding activity and comprising either 2 or 3 RNA 10 recognition motifs (RRMs) and a motif having at least 75% sequence identity to motif I: PIYEAAVVALPVVVKERLVRILRLGIATRYD and/or a motif having at least 50% sequence identity to motif II: RFDPFTGEPYKFDP; or (ii) an RBP1 polypeptide or homologue thereof having (a) RNA-binding activity; (b) two RRM domains, (c) the following two motifs: (i) KIFVGGL; and (ii) 15 RPRGFGF, allowing for up to three amino acid substitutions and any conservative change in the motifs; and (d) having at least 20% sequence identity to the amino acid represented by SEQ ID NO: 15. The invention also concerns to transgenic plants having introduced therein an RNA-binding protein-encoding nucleic acid or variant thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The present invention also concerns constructs useful in the methods of the invention.
    • 本发明涉及通过增加植物中的RNA结合蛋白或其同源物的活性来改善植物的生长特性的方法,其中所述RNA结合蛋白或其同源物是:(i)具有RNA结合蛋白的多肽, 包含2或3个RNA 10识别基序(RRM)和与基序I具有至少75%序列同一性的基序:PIYEAAVVALPVVKERLVRILRLGIATRYD和/或与基序II具有至少50%序列同一性的基序:RFDPFTGEPYKFDP; 或(ii)具有(a)RNA结合活性的RBP1多肽或其同系物; (b)两个RRM域,(c)以下两个图案:(i)KIFVGGL; 和(ii)15种RPRGFGF,允许多达3个氨基酸取代和图案中任何保守的变化; 和(d)与SEQ ID NO:15所示的氨基酸具有至少20%的序列同一性。本发明还涉及其中引入了编码有RNA结合蛋白的核酸或其变体的转基因植物,其植物已经改进 相对于相应的野生型植物的生长特性。 本发明还涉及可用于本发明方法的构建体。
    • 62. 发明申请
    • PLANTS HAVING ENHANCED YIELD-RELATED TRAITS AND METHOD FOR MAKING THE SAME
    • 具有增强的相关行为的植物及其制造方法
    • US20130036516A1
    • 2013-02-07
    • US13635583
    • 2011-03-17
    • Yves HatzfeldChristophe Reuzeau
    • Yves HatzfeldChristophe Reuzeau
    • A01H5/00C12N5/10A01H5/06A01H5/10C12N15/82A01H5/04
    • C12N15/8261C07K14/415Y02A40/146
    • The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a CLE-type 2 polypeptide or a Bax Inhibitor-1 (BI-1) polypeptide or a SEC22 polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a CLE-type 2 polypeptide or a BI-1 polypeptide or a SEC22 polypeptide, which plants have enhanced yield-related traits compared with control plants. The invention also provides constructs comprising CLE-type 2-encoding nucleic acids, useful in performing the methods of the invention. The invention also provides novel BI-1-encoding nucleic acids and constructs comprising the same, useful in performing the methods of the invention. The invention also provides novel SEC22-encoding nucleic acids and constructs comprising the same, useful in performing the methods of the invention.
    • 本发明一般涉及分子生物学领域,涉及增强植物中各种经济上重要的产量相关性状的方法。 更具体地,本发明涉及通过调节植物中编码CLE-2型多肽或Bax抑制剂-1(BI-1)多肽或SEC22多肽的核酸表达来增强植物中产量相关性状的方法 。 本发明还涉及具有调节表达编码CLE型2多肽或BI-1多肽或SEC22多肽的核酸的植物,与对照植物相比,植物具有增强的产量相关性状。 本发明还提供了包含CLE2型编码核酸的构建体,其可用于实施本发明的方法。 本发明还提供了新颖的编码BI-1的核酸和包含其的构建体,其可用于实施本发明的方法。 本发明还提供了新颖的编码SEC22的核酸和包含该核酸的构建体,其可用于实施本发明的方法。