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    • 67. 发明授权
    • Monoclonal antibody against hepatitis E virus or its fragment with binding activity and use thereof
    • 针对戊型肝炎病毒的单克隆抗体或其具有结合活性的片段及其用途
    • US09428554B2
    • 2016-08-30
    • US12433497
    • 2009-04-30
    • Ningshao XiaJun ZhangYing GuShaowei LiShengxiang GeZhiqiang He
    • Ningshao XiaJun ZhangYing GuShaowei LiShengxiang GeZhiqiang He
    • C07K14/005A61K39/29C07K16/10A61K39/00G01N33/576A61K39/12
    • C07K14/005A61K39/00A61K39/12A61K39/29A61K2039/545A61K2039/55505A61K2039/55566A61K2039/57C07K16/10C12N2770/28122C12N2770/28134G01N33/576
    • The present invention relates to monoclonal antibody specifically binding to polypeptide(s) comprising the amino acid sequence as set forth in SEQ ID No. 1 of hepatitis E virus ORF2 or its conserved variants or its active fragments, or other monoclonal antibodies against ORF2 which can cross react with said monoclonal antibody of present invention, and its nucleotide sequence or its degenerate sequence; to the antigenic determinant in hepatitis E virus ORF2; to a method for screening isolated or recombined polypeptide or polypeptide analog, which has the same property of specifically binding said monoclonal antibody 8C11 and/or 8H3 as said antigenic determinant 1) or 3) of hepatitis E virus ORF2; to polypeptide or polypeptide analog screened by the method above and its nucleotide sequence or degenerate sequence; to a use of said polypeptide or polypeptide analog in preparation of a medicament for the diagnosis and/or precaution of hepatitis E virus infection; to a diagnostic kit for hepatitis E virus infection and a vaccine composition for prophylaxis of hepatitis E virus infection; to use of said monoclonal antibodies or their active fragments or conserved variants in preparation of a medicament for diagnosis, prophylaxis and/or treatment of hepatitis E virus infection; to pharmaceutical composition for prophylaxis and/or treatment of hepatitis E virus infection and a method for prophylaxis and/or treatment of hepatitis E virus infection; to a recombinant expression vector comprising said nucleotide molecule in present invention and a host cell transformed with said recombinant expression vector that is able to express monoclonal antibody and its conserved variants or active fragments or polypeptide or polypeptide analogs.
    • 本发明涉及与多肽特异性结合的单克隆抗体,其包含如戊型肝炎病毒ORF2的SEQ ID No.1所示的氨基酸序列或其保守变体或其活性片段或针对ORF2的其它单克隆抗体,其可以 与本发明的单克隆抗体交叉反应,其核苷酸序列或其简并序列; 对乙型肝炎病毒ORF2的抗原决定簇; 涉及用于筛选分离或重组的多肽或多肽类似物的方法,其具有与所述单克隆抗体8C11和/或8H3特异性结合作为所述抗原决定簇1)或3)的戊型肝炎病毒ORF2的相同性质; 通过上述方法筛选的多肽或多肽模拟物及其核苷酸序列或简并序列; 涉及所述多肽或多肽类似物在制备用于诊断和/或预防戊型肝炎病毒感染的药物中的用途; 用于戊型肝炎病毒感染的诊断试剂盒和用于预防戊型肝炎病毒感染的疫苗组合物; 在制备用于诊断,预防和/或治疗戊型肝炎病毒感染的药物中使用所述单克隆抗体或其活性片段或保守变体; 用于预防和/或治疗戊型肝炎病毒感染的药物组合物和预防和/或治疗戊型肝炎病毒感染的方法; 涉及包含本发明中的所述核苷酸分子和能够表达单克隆抗体及其保守变体或活性片段或多肽或多肽类似物的所述重组表达载体转化的宿主细胞的重组表达载体。