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61. 发明申请

US20110201507A1 SEQUENCING METHODS AND COMPOSITIONS FOR PRENATAL DIAGNOSES 有权
标题翻译：用于初步诊断的顺序方法和组合
公开(公告)号：US20110201507A1
公开(公告)日：2011-08-18
申请号：US12958353
申请日：2010-12-01
申请人： Richard P. Rava , Manjula Chinnappa , David A. Comstock , Gabrielle Heilek , Brian Kent Rhees
发明人： Richard P. Rava , Manjula Chinnappa , David A. Comstock , Gabrielle Heilek , Brian Kent Rhees
IPC分类号： C40B30/00 , C40B60/08 , G06F19/00
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6809 , C12Q1/6883 , C12Q2600/106 , C12Q2537/16 , C12Q2537/165 , C12Q2545/101
摘要： The invention provides methods for determining aneuploidy and/or fetal fraction in maternal samples comprising fetal and maternal cfDNA by massively parallel sequencing. The method comprises a novel protocol for preparing sequencing libraries that unexpectedly improves the quality of library DNA while expediting the process of analysis of samples for prenatal diagnoses.
摘要翻译：本发明提供了通过大规模平行测序来测定母体样品中包括胎儿和母体cfDNA的非整倍体和/或胎儿部分的方法。该方法包括用于制备测序文库的新方案，其意外地提高文库DNA的质量，同时加速用于产前诊断的样品分析过程。

62. 发明申请

US20100280234A1 Photocleavable Protecting Groups and Methods for Their Use 审中-公开
标题翻译：可剥离保护组及其使用方法
公开(公告)号：US20100280234A1
公开(公告)日：2010-11-04
申请号：US12343782
申请日：2008-12-24
申请人： Glenn H. McGall , Ngo Q. Nam , Richard P. Rava
发明人： Glenn H. McGall , Ngo Q. Nam , Richard P. Rava
IPC分类号： C07H19/10
CPC分类号： C07H21/00 , B01J2219/00432 , C07B2200/11 , C07H19/10 , C07H19/20 , C07H19/207 , C07K1/061 , C40B40/04 , C40B50/14 , C40B60/14 , G01N33/54353 , Y02P20/55
摘要： Novel compounds are provided which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Ar—C(R1)(R2)—O—C(O)— wherein: Ar is an optionally substituted fused polycyclic aryl or heteroaromatic group or a vinylogous derivative thereof; R1 and R2 are independently H, optionally substituted alkyl, alkenyl or alkynyl, optionally substituted aryl or optionally substituted heteroaromatic, or a vinylogous derivative of the foregoing; and X is a leaving group, a chemical fragment linked to Ar—C(R1)(R2)—O—C(O)— via a heteroatom, or a solid support; provided that when Ar is 1-pyrenyl and R1 and R2 are H, X is not linked to Ar—C(R1)(R2)—O—C(O)— via a nitrogen atom. Preferred embodiments are those in which Ar is a fused polycyclic aromatic hydrocarbon and in which the substituents on Ar, R1 and R2 are electron donating groups. A particularly preferred protecting group is the “PYMOC” protecting group, pyrenylmethyloxycarbonyl, where Ar is pyrenyl and R1 and R2 are H. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.
摘要翻译：提供了新的化合物，其可用作化学合成中的连接基团，优选在寡核苷酸和多肽的固相合成中。这些化合物通常是光不稳定的并且包括可以通过光解去除以揭示反应性基团的保护基团。保护基具有通式Ar-C（R 1）（R 2）-O-C（O） - ，其中：Ar是任选取代的稠合多环芳基或杂芳族基团或其杂原子衍生物; R 1和R 2独立地为H，任选取代的烷基，烯基或炔基，任选取代的芳基或任选取代的杂芳族化合物，或前述的乙烯衍生物; X是离去基团，经由杂原子连接到Ar-C（R 1）（R 2）-O-C（O） - 或固体载体上的化学片段; 条件是当Ar为1-芘基且R 1和R 2为H时，X不能通过氮原子与Ar-C（R 1）（R 2）-O-C（O） - 连接。优选的实施方案是其中Ar是稠合多环芳烃并且其中Ar，R 1和R 2上的取代基是给电子基团的实施方案。特别优选的保护基是“PYMOC”保护基，芘基甲氧基羰基，其中Ar是芘基，R 1和R 2是H.还提供了一种从组分分子在载体上形成多种化合物的方法，每种化合物占据使用上述保护的化合物分离预定区域的载体。

63. 发明授权

US07470783B2 Photocleavable protecting groups and methods for their use 有权
标题翻译：可透光保护组及其使用方法
公开(公告)号：US07470783B2
公开(公告)日：2008-12-30
申请号：US11105571
申请日：2005-04-14
申请人： Glenn H. McGall , Ngo Q. Nam , Richard P. Rava
发明人： Glenn H. McGall , Ngo Q. Nam , Richard P. Rava
IPC分类号： C07H21/00
CPC分类号： C07H21/00 , B01J2219/00432 , C07B2200/11 , C07H19/10 , C07H19/20 , C07H19/207 , C07K1/061 , C40B40/04 , C40B50/14 , C40B60/14 , G01N33/54353 , Y02P20/55
摘要： Novel compounds are provided which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Ar—C(R1)(R2)—O—C(O)-wherein: Ar is an optionally substituted fused polycyclic aryl or heteroaromatic group or a vinylogous derivative thereof; R1 and R2 are independently H, optionally substituted alkyl, alkenyl or alkynyl, optionally substituted aryl or optionally substituted heteroaromatic, or a vinylogous derivative of the foregoing; and X is a leaving group, a chemical fragment linked to Ar—C(R1)(R2)—O—C(O)— via a heteroatom, or a solid support; provided that when Ar is 1-pyrenyl and R1 and R2 are H, X is not linked to Ar—C(R1)(R2)—O—C(O)— via a nitrogen atom. Preferred embodiments are those in which Ar is a fused polycyclic aromatic hydrocarbon and in which the substituents on Ar, R1 and R2 are electron donating groups. A particularly preferred protecting group is the “PYMOC” protecting group, pyrenylmethyloxycarbonyl, where Ar is pyrenyl and R1 and R2 are H. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.
摘要翻译：提供了新的化合物，其可用作化学合成中的连接基团，优选在寡核苷酸和多肽的固相合成中。这些化合物通常是光不稳定的并且包括可以通过光解去除以揭示反应性基团的保护基团。保护基具有通式Ar-C（R 1）（R 2）-O-C（O） - ，其中：Ar是任选取代的稠合多环芳基或杂芳族基团或其杂原子衍生物; R 1和R 2独立地为H，任选取代的烷基，烯基或炔基，任选取代的芳基或任选取代的杂芳族化合物，或前述的乙烯衍生物; X是离去基团，经由杂原子连接到Ar-C（R 1）（R 2）-O-C（O） - 或固体载体上的化学片段; 条件是当Ar为1-芘基且R 1和R 2为H时，X不能通过氮原子与Ar-C（R 1）（R 2）-O-C（O） - 连接。优选的实施方案是其中Ar是稠合多环芳烃并且其中Ar，R 1和R 2上的取代基是给电子基团的实施方案。特别优选的保护基是“PYMOC”保护基，芘基甲氧基羰基，其中Ar是芘基，R 1和R 2是H.还提供了一种从组分分子在载体上形成多种化合物的方法，每种化合物占据使用上述保护的化合物分离预定区域的载体。

64. 发明授权

US06881836B2 Photocleavable protecting groups and methods for their use 失效
标题翻译：可透光保护组及其使用方法
公开(公告)号：US06881836B2
公开(公告)日：2005-04-19
申请号：US10348917
申请日：2003-01-22
申请人： Glenn H. McGall , Ngo Q. Nam , Richard P. Rava
发明人： Glenn H. McGall , Ngo Q. Nam , Richard P. Rava
IPC分类号： B05D3/00 , C07B61/00 , C07H19/10 , C07H19/20 , C07H19/207 , C07H21/00 , C07K1/06 , C40B60/14 , G01N33/543
CPC分类号： C07H21/00 , B01J2219/00432 , C07B2200/11 , C07H19/10 , C07H19/20 , C07H19/207 , C07K1/061 , C40B40/04 , C40B50/14 , C40B60/14 , G01N33/54353 , Y02P20/55
摘要： Novel compounds are provided which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Ar—C(R1)(R2)—O—C(O)— wherein: Ar is an optionally substituted fused polycyclic aryl or heteroaromatic group or a vinylogous derivative thereof; R1 and R2 are independently H, optionally substituted alkyl, alkenyl or alkynyl, optionally substituted aryl or optionally substituted heteroaromatic, or a vinylogous derivative of the foregoing; and X is a leaving group, a chemical fragment linked to Ar—C(R1)(R2)—O—C(O)— via a heteroatom, or a solid support; provided that when Ar is 1-pyrenyl and R1 and R2 are H, X is not linked to Ar—C(R1)(R2)—O—C(O)— via a nitrogen atom. Preferred embodiments are those in which Ar is a fused polycyclic aromatic hydrocarbon and in which the substituents on Ar, R1 and R2 are electron donating groups. A particularly preferred protecting group is the “PYMOC” protecting group, pyrenylmethyloxycarbonyl, where Ar is pyrenyl and R1 and R2 are H. Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.
摘要翻译：提供了新的化合物，其可用作化学合成中的连接基团，优选在寡核苷酸和多肽的固相合成中。这些化合物通常是光不稳定的并且包括可以通过光解去除以揭示反应性基团的保护基团。保护基具有通式Ar-C（R 1）（R 2） - OC（O） - ，其中：Ar是任选取代的稠合多环芳基或杂芳族组或其衍生物; R 1和R 2独立地为H，任选取代的烷基，烯基或炔基，任选取代的芳基或任选取代的杂芳族，或前述的维果衍生物; X是离去基团，经由杂原子与Ar-C（R 1）（R 2）-O（O） - 连接的化学片段或固体支持; 条件是当Ar为1-芘基且R 1和R 2为H时，X不与Ar-C（R 1）（R 2）-O（O） - 经由氮原子。优选的实施方案是其中Ar是稠合多环芳烃并且其中Ar 1，R 1和R 2上的取代基是给电子基团的实施方案。特别优选的保护基是“PYMOC”保护基，芘基甲氧基羰基，其中Ar是芘基，R 1和R 2都是H.还提供了一种形成，使用上述保护的化合物，从成分分子，载体上的多种化合物，每种化合物占据支持体的单独的预定区域。

65. 发明授权

US6147205A Photocleavable protecting groups and methods for their use 失效
公开(公告)号：US6147205A
公开(公告)日：2000-11-14
申请号：US812005
申请日：1997-03-05
申请人： Glenn H. McGall , Ngo Q. Nam , Richard P. Rava
发明人： Glenn H. McGall , Ngo Q. Nam , Richard P. Rava
IPC分类号： B05D3/00 , C07B61/00 , C07H19/10 , C07H19/20 , C07H19/207 , C07H21/00 , C07K1/06 , C40B60/14 , G01N33/543 , C07H19/00
CPC分类号： C07H21/00 , C07H19/10 , C07H19/20 , C07H19/207 , C07K1/061 , C40B50/14 , G01N33/54353 , B01J2219/00432 , C07B2200/11 , C40B40/04 , C40B60/14 , Y02P20/55
摘要： Novel compounds are provided which are useful as linking groups in chemical synthesis, preferably in the solid phase synthesis of oligonucleotides and polypeptides. These compounds are generally photolabile and comprise protecting groups which can be removed by photolysis to unmask a reactive group. The protecting group has the general formula Ar--C(R.sub.1)(R.sub.2)--O--C(O)-- wherein:Ar is an optionally substituted fused polycyclic aryl or heteroaromatic group or a vinylogous derivative thereof;R.sub.1 and R.sub.2 are independently H, optionally substituted alkyl, alkenyl or alkynyl, optionally substituted aryl or optionally substituted heteroaromatic, or a vinylogous derivative of the foregoing; andX is a leaving group, a chemical fragment linked to Ar--C(R.sub.1)(R.sub.2)--O--C(O)-- via a heteroatom, or a solid support; provided that when Ar is 1-pyrenyl and R.sub.1 and R.sub.2 are H, X is not linked to Ar--C(R.sub.1)(R.sub.2)--O--C(O)-- via a nitrogen atom. Preferred embodiments are those in which Ar is a fused polycyclic aromatic hydrocarbon and in which the substituents on Ar, R.sub.1 and R.sub.2 are electron donating groups. A particularly preferred protecting group is the "PYMOC" protecting group, pyrenylmethyloxycarbonyl, where Ar is pyrenyl and R.sub.1 and R.sub.2 are H.Also provided is a method of forming, from component molecules, a plurality of compounds on a support, each compound occupying a separate predefined region of the support, using the protected compounds described above.

66. 发明授权

US5874219A Methods for concurrently processing multiple biological chip assays 失效
标题翻译：同时处理多种生物芯片测定方法
公开(公告)号：US5874219A
公开(公告)日：1999-02-23
申请号：US630051
申请日：1996-04-09
申请人： Richard P. Rava , Stephen P.A. Fodor , Mark Trulson
发明人： Richard P. Rava , Stephen P.A. Fodor , Mark Trulson
IPC分类号： B01J19/00 , B01L3/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N21/64 , G01N35/02 , C12P19/34
CPC分类号： G01N35/028 , B01J19/0046 , B01L3/5085 , C12Q1/6837 , B01J2219/00315 , B01J2219/00317 , B01J2219/00432 , B01J2219/00529 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01J2219/00702 , B01J2219/00707 , B01J2219/00722 , B01L2300/0636 , B01L2300/0829 , C40B40/06 , C40B60/14 , G01N21/64
摘要： Methods for concurrently processing multiple biological chip assays by providing a biological chip plate comprising a plurality of test wells, each test well having a biological chip having a molecular probe array; introducing samples into the test wells; subjecting the biological chip plate to manipulation by a fluid handling device that automatically performs steps to carry out reactions between target molecules in the samples and probes; and subjecting the biological chip plate to a biological chip plate reader that interrogates the probe arrays to detect any reactions between target molecules and probes.
摘要翻译：通过提供包括多个测试孔的生物芯片板同时处理多个生物芯片测定的方法，每个测试孔具有具有分子探针阵列的生物芯片; 将样品引入测试井; 使生物芯片板通过流体处理装置进行操作，所述流体处理装置自动执行步骤以进行样品和探针中的靶分子之间的反应; 并将生物芯片板经受询问探针阵列的生物芯片读板器，以检测靶分子和探针之间的任何反应。

67. 发明授权

US5419323A Method for laser induced fluorescence of tissue 失效
标题翻译：激光诱导组织荧光的方法
公开(公告)号：US5419323A
公开(公告)日：1995-05-30
申请号：US720465
申请日：1991-08-29
申请人： Carter Kittrell , Robert M. Cothren , Michael S. Feld , Joseph J. Baraga , Kyungwon An , Rebecca Richards-Kortum , Richard P. Rava , Young D. Park , Anand Mehta , Paola Taroni , Lucene Tong , Ramachandra R. Dasari
发明人： Carter Kittrell , Robert M. Cothren , Michael S. Feld , Joseph J. Baraga , Kyungwon An , Rebecca Richards-Kortum , Richard P. Rava , Young D. Park , Anand Mehta , Paola Taroni , Lucene Tong , Ramachandra R. Dasari
IPC分类号： A61B5/00 , A61B18/24 , A61B6/00
CPC分类号： A61B5/0084 , A61B18/245 , A61B5/0071 , A61B5/0075 , A61B5/1459 , A61B2562/0238
摘要： A method for laser induced fluorescence of tissue in which laser radiation is used to illuminate and induce fluorescence in the tissue under study to determine the chemical composition or pathologic condition of tissue. The laser radiation and the retrieved fluorescing radiation can be conveyed through a catheter using an array of optical fiber. The fluorescence spectrum of the tissue can be displayed and analyzed to obtain information regarding the chemical composition and medical condition of the tissue inside the human body.
摘要翻译： PCT No.PCT / US89 / 05174 Sec。 371日期：1993年5月7日 102（e）日期1993年5月7日PCT提交1989年11月17日PCT公布。出版物WO90 / 06718 日期：1990年6月28日。一种激光诱导荧光组织的方法，其中使用激光辐射来照射和诱导研究组织中的荧光以确定组织的化学组成或病理状况。激光辐射和所检索的荧光辐射可以使用光纤阵列通过导管输送。可以显示和分析组织的荧光光谱，以获得关于人体内组织的化学成分和医疗状况的信息。

68. 发明授权

US5345941A Contour mapping of spectral diagnostics 失效
标题翻译：光谱诊断的轮廓映射
公开(公告)号：US5345941A
公开(公告)日：1994-09-13
申请号：US24674
申请日：1993-03-01
申请人： Richard P. Rava , Rebecca Richards-Kortum , Michael S. Feld , Joseph J. Baraga
发明人： Richard P. Rava , Rebecca Richards-Kortum , Michael S. Feld , Joseph J. Baraga
IPC分类号： A61B5/00 , A61B6/00
CPC分类号： G01J3/4406 , A61B5/0059 , G01N21/6456 , G01N2021/6417 , G01N2021/6419 , G01N2021/6484
摘要： The present invention relates to a method of generating and processing spectral information arising from the laser induced fluorescence of tissue. The intensity of fluorescence is recorded as a function of both excitation and emission wavelengths and contour maps of tissue fluorescence are generated which are useful in the diagnosis of condition of the tissue under examination.
摘要翻译：本发明涉及一种生成和处理由激光诱导的组织荧光产生的光谱信息的方法。记录荧光的强度作为激发和发射波长的函数，并且产生组织荧光的轮廓图，其可用于诊断被检组织的状况。

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