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61. 发明授权

US06261551B1 Recombinant adenovirus and adeno-associated virus, cell lines, and methods of production and use thereof 失效
标题翻译：重组腺病毒和腺相关病毒，细胞系及其生产和使用方法
公开(公告)号：US06261551B1
公开(公告)日：2001-07-17
申请号：US08973334
申请日：1997-12-05
申请人： James M. Wilson , Krishna J. Fisher , Guang-Ping Gao
发明人： James M. Wilson , Krishna J. Fisher , Guang-Ping Gao
IPC分类号： A61K4800
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10343 , C12N2750/14143 , Y10S977/802 , Y10S977/907
摘要： An adenovirus E1/E4 expressing packaging cell line is provided, which permits the generation of recombinant adenoviruses deleted in both gene regions. A method for enhancing the efficiency of transduction of a recombinant AAV into a target cell is provided by infecting a target cell with a recombinant AAV comprising a selected transgene under the control of regulatory sequences. The infected cell is contacted with an agent which facilitates the conversion of single stranded recombinant virus to its double stranded form.
摘要翻译：提供了表达腺病毒E1 / E4的包装细胞系，其允许在两个基因区域中产生缺失的重组腺病毒。通过在包含调节序列控制下的选择的转基因的重组AAV感染靶细胞来提供将重组AAV转导进靶细胞的效率的方法。将感染的细胞与促进单链重组病毒转化为其双链形式的试剂接触。

62. 发明授权

US06258595B1 Compositions and methods for helper-free production of recombinant adeno-associated viruses 有权
标题翻译：无辅助生产重组腺相关病毒的组合物和方法
公开(公告)号：US06258595B1
公开(公告)日：2001-07-10
申请号：US09404555
申请日：1999-09-23
申请人： Guang-Ping Gao , James M. Wilson
发明人： Guang-Ping Gao , James M. Wilson
IPC分类号： C12N1586
CPC分类号： G01R31/275 , C12N15/86 , C12N2750/14143 , C12N2750/14151 , C12N2840/20 , G01R31/013 , G01R31/316
摘要： A method for producing recombinant adeno-associated virus in the absence of contaminating helper virus or wild-type virus involves culturing a mammalian host cell containing an rAd/AAV hybrid virus, an AAV rep sequence and an AAV cap sequence under the control of regulatory sequences directing expression thereof. The rAd/AAV hybrid virus contains a rAAV construct to be packaged into an AAV virion in a backbone containing the adenoviral sequences necessary to express E1a and E1b gene products and to permit replication of the hybrid virus. The method of the invention permits replication of the hybrid virus and production of rAAV virion in this host cell in the absence of a helper virus and obviates a subsequent purification step to purify rAAV from contaminating virus.
摘要翻译：在不存在污染性辅助病毒或野生型病毒的情况下用于产生重组腺相关病毒的方法包括在调节序列的控制下培养含有rAd / AAV杂合病毒，AAV rep序列和AAV帽序列的哺乳动物宿主细胞指导其表达。 rAd / AAV杂合病毒含有一个rAAV构建体，其被包装在含有表达E1a和E1b基因产物所必需的腺病毒序列并且允许杂交病毒复制的骨架中的AAV病毒粒子中。本发明的方法允许在不存在辅助病毒的情况下复制该杂种病毒并在该宿主细胞中产生rAAV病毒粒子，并且消除随后的纯化步骤以从污染病毒纯化rAAV。

63. 发明授权

US06174527B1 Methods and compositions for gene therapy for the treatment of defects in lipoprotein metabolism 失效
标题翻译：用于治疗脂蛋白代谢缺陷的基因治疗方法和组合物
公开(公告)号：US06174527B1
公开(公告)日：2001-01-16
申请号：US08894489
申请日：1997-10-24
申请人： James M. Wilson , Karen Kozarsky , Jerome Strauss, III
发明人： James M. Wilson , Karen Kozarsky , Jerome Strauss, III
IPC分类号： A61K4800
CPC分类号： C12N15/86 , A61K38/00 , A61K48/00 , C07K14/705 , C12N2710/10344 , C12N2799/022
摘要： The invention provides a recombinant viral vector comprising a human VLDL receptor gene operatively linked to regulatory sequences directing its expression. The vector is capable of expressing the normal VLDL receptor gene product in hepatic cells in vivo or in vitro. This viral vector is usefuil in the treatment of metabolic disorders caused by the accumulation of LDL in plasma, such as familial hypercholesterolemia or familial combined hyperlipidemia.
摘要翻译：本发明提供了包含与指导其表达的调节序列有效连接的人VLDL受体基因的重组病毒载体。该载体能够在体内或体外在肝细胞中表达正常的VLDL受体基因产物。该病毒载体是用于治疗血浆中LDL积累引起的代谢紊乱，如家族性高胆固醇血症或家族性高脂血症。

64. 发明授权

US6001557A Adenovirus and methods of use thereof 失效
标题翻译：腺病毒及其使用方法
公开(公告)号：US6001557A
公开(公告)日：1999-12-14
申请号：US836022
申请日：1997-08-25
申请人： James M. Wilson , Krishna J. Fisher , Shu-Jen Chen , Matthew Weitzman
发明人： James M. Wilson , Krishna J. Fisher , Shu-Jen Chen , Matthew Weitzman
IPC分类号： C12N15/09 , A61K35/76 , A61K48/00 , C12N7/00 , C12N15/861 , C12Q1/70 , C12N15/64 , C12N15/86
CPC分类号： C12N7/00 , C12N15/86 , A61K48/00 , C12N2710/10343 , C12N2810/6018 , C12N2840/44
摘要： A recombinant adenovirus and a method for producing the virus are provided which utilize a recombinant shuttle vector comprising adenovirus DNA sequence for the 5' and 3' cis-elements necessary for replication and virion encapsidation in the absence of sequence encoding viral genes and a selected minigene linked thereto, and a helper adenovirus comprising sufficient adenovirus gene sequences necessary for a productive viral infection. Desirably, the helper gene is crippled by modifications to its 5' packaging sequences, which facilitates purification of the viral particle from the helper virus.
摘要翻译： PCT No.PCT / US95 / 14017 Sec。 371日期1997年8月25日第 102（e）日期1997年8月25日PCT提交1995年10月27日PCT公布。公开号WO96 / 13597 日期1996年5月9日提供了一种重组腺病毒及其生产方法，其在不存在编码病毒基因的序列的情况下，利用包含腺病毒DNA序列的重组穿梭载体用于复制所必需的5'和3'顺式元件和病毒粒子壳化和与其连接的选择的小基因，以及包含产生性病毒感染所必需的足够的腺病毒基因序列的辅助性腺病毒。期望的是，辅助基因通过修饰其5'包装序列而被削弱，这有助于从辅助病毒纯化病毒颗粒。

65. 发明授权

US5866552A Method for expressing a gene in the absence of an immune response 失效
标题翻译：在不存在免疫应答的情况下表达基因的方法
公开(公告)号：US5866552A
公开(公告)日：1999-02-02
申请号：US708188
申请日：1996-09-06
申请人： James M. Wilson , Krishna J. Fisher
发明人： James M. Wilson , Krishna J. Fisher
IPC分类号： A61K48/00 , C12N15/864 , C12N15/63 , A01N43/04 , A61K31/70
CPC分类号： C12N15/86 , A61K48/00 , C12N2750/14143
摘要： A method of gene transfer involving administering a recombinant adeno-associated virus (AAV) bearing the desired gene into the muscle of the animal is described.
摘要翻译：描述了涉及将携带所需基因的重组腺相关病毒（AAV）给予动物的肌肉的基因转移方法。

66. 发明授权

US5585362A Adenovirus vectors for gene therapy 失效
标题翻译：用于基因治疗的腺病毒载体
公开(公告)号：US5585362A
公开(公告)日：1996-12-17
申请号：US73354
申请日：1993-06-07
申请人： James M. Wilson , John Engelhardt
发明人： James M. Wilson , John Engelhardt
IPC分类号： C12N15/09 , A01K67/027 , A61K31/70 , A61K35/76 , A61K38/00 , A61K48/00 , A61L27/38 , C07K14/47 , C07K16/18 , C07K16/28 , C12N15/00 , C12N15/85 , C12N15/861 , C12R1/92 , C12N5/16 , C12N15/63
CPC分类号： C12N15/86 , A01K67/027 , A01K67/0271 , A61L27/38 , B82Y5/00 , C07K14/4712 , C07K16/18 , C07K16/28 , C12N15/8509 , A01K2217/05 , A01K2267/03 , A61K38/00 , A61K48/00 , C12N2710/10343
摘要： The present invention comprises an improved adenovirus vector and methods for making and using such vectors. The adenovirus vectors of the present invention retain at least a portion of the adenoviral E3 region, carry a deletion of at least a portion of the adenoviral E1 region. Vectors of the present invention preferably also include an additional deletion to accommodate a transgene and/or other mutations which result in reduced expression or over-expression of adenoviral protein and/or reduced viral replication. The vectors of the present invention further include a transgene operatively-linked thereto. By reducing or eliminating viral replication and viral protein expression, the immune response of the infected host to the virus and viral protein is decreased and persistence of transgene expression can be increased. The adenovirus vectors of the present invention are thus particularly useful in gene transfer and therapy.
摘要翻译：本发明包括改良的腺病毒载体和用于制备和使用这些载体的方法。本发明的腺病毒载体保留腺病毒E3区域的至少一部分，携带至少部分腺病毒E1区域的缺失。本发明的载体还优选还包括另外的缺失以适应导致腺病毒蛋白质的表达或过度表达和/或减少的病毒复制的转基因和/或其它突变。本发明的载体还包括与其可操作地连接的转基因。通过减少或消除病毒复制和病毒蛋白表达，感染的宿主对病毒和病毒蛋白的免疫应答降低，可以增加转基因表达的持续性。因此，本发明的腺病毒载体特别可用于基因转移和治疗。

67. 发明授权

US5455708A Passive scan angle doubling optical system 失效
标题翻译：被动扫描角倍增光学系统
公开(公告)号：US5455708A
公开(公告)日：1995-10-03
申请号：US357881
申请日：1994-12-19
申请人： Ellis D. Harris , James M. Wilson
发明人： Ellis D. Harris , James M. Wilson
IPC分类号： G02B26/10 , G02B26/08 , G02B5/18
CPC分类号： G02B26/106
摘要： A scan angle doubling system using a rotating cylindrical drum with reflective binary diffractive optical element facets, a cylindrical mirror, and a lens to increase the scanning angle available from a rotating mirror polygon of a given size. The properties of the passive components are such that the output light beam from the reflective binary diffractive optical element facets of the rotating cylindrical drum is collimated and normal to the rotating cylindrical drum. The cylindrical drum, cylindrical mirror, and lens are arranged such that the rotating cylindrical drum and the cylindrical mirror are opposed to each other and separated by a distance greater than the sum of the radius of curvature of the cylindrical mirror plus the focal length of the lens, and the lens is interposed between the rotating cylindrical drum and the cylindrical mirror so that the center of the radius of curvature of the cylindrical mirror and the focal point of the lens means are coincident.
摘要翻译：使用具有反射二元衍射光学元件面的旋转圆柱形鼓的扫描角倍增系统，圆柱形镜和透镜，以增加从给定尺寸的旋转镜多边形可获得的扫描角度。无源部件的性质使得来自旋转圆柱形鼓的反射二元衍射光学元件面的输出光束被准直并垂直于旋转的圆柱形鼓。圆柱形鼓，柱面镜和透镜被布置成使得旋转的圆柱形滚筒和圆柱形反射镜彼此相对并分开大于圆柱形反射镜的曲率半径与焦距的总和的距离透镜，并且透镜被插入在旋转圆柱形滚筒和圆柱形反射镜之间，使得圆柱形反射镜的曲率半径的中心和透镜装置的焦点一致。

68. 发明授权

US4833846A Ground anchor system for supporting an above ground structure 失效
标题翻译：支撑地面结构的地面锚系统
公开(公告)号：US4833846A
公开(公告)日：1989-05-30
申请号：US153368
申请日：1988-02-08
申请人： James McFeetors , James M. Wilson
发明人： James McFeetors , James M. Wilson
IPC分类号： E04H12/20
CPC分类号： E04H12/20
摘要： A device for supporting a building structure or the like includes an elongate shaft which is of square cross section and has a short length of screw flight at a lower end and the male screw threaded portion at an upper end extending coaxially with the shaft. A stabilizer in the form of a sleeve arranged to closely surround the square shaft together with three wings to be hammered downwardly into the ground surrounding the shaft after the shaft is screwed into the ground. Various heads can be provided including particularly a building support head in the form of a sleeve with a female screw thread at one end for attachment to the threaded portion of the shaft and a plate separate from the sleeve which can swivel relative to the sleeve to allow adjustment of the height.
摘要翻译：用于支撑建筑物结构等的装置包括细长轴，该细长轴具有正方形横截面并且在下端处具有短长度的螺旋螺纹，并且在与轴同轴延伸的上端处的外螺纹部分。套筒形式的稳定器被布置成紧密地围绕方轴，同时三个翼片在轴被拧入地面之后向下被锤击到围绕轴的地面中。可以提供各种头部，特别是具有套筒形式的建筑支撑头，其一端具有内螺纹，用于连接到轴的螺纹部分，以及与套筒分离的板，该板可以相对于套筒旋转以允许调整高度。

69. 发明授权

US08940290B2 Simian adenoviruses SAdV-43, -45, -46, -47, -48, -49, and -50 and uses thereof 有权
标题翻译：猿猴腺病毒SAdV-43，-45，-46，-47，-48，-49和-50及其用途
公开(公告)号：US08940290B2
公开(公告)日：2015-01-27
申请号：US13126557
申请日：2009-10-29
申请人： Soumitra Roy , James M. Wilson , Luc H. Vandenberghe
发明人： Soumitra Roy , James M. Wilson , Luc H. Vandenberghe
IPC分类号： A61K35/76 , C12N15/11 , C12N15/63 , C12N15/33
CPC分类号： C12N15/86 , C12N2710/10321 , C12N2710/10322 , C12N2710/10341 , C12N2710/10342 , C12N2710/10343 , C12N2710/10345 , C12N2710/10352 , C12N2810/6018
摘要： A recombinant vector comprises simian adenovirus 43, 45, 46, 47, 48, 49 or 50 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus 43, 45, 46, 47, 48, 49 or 50 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
摘要翻译：重组载体包含猿猴腺病毒43,45,46,47,48,49或50个序列，以及在调控序列控制下的异源基因。还公开了表达猿腺病毒43,45,46,47,48,49或50个基因的细胞系。提供了使用载体和细胞系的方法。

70. 发明授权

US08846031B2 Simian adenovirus 41 and uses thereof 有权
标题翻译：猿猴腺病毒41及其用途
公开(公告)号：US08846031B2
公开(公告)日：2014-09-30
申请号：US13321985
申请日：2010-05-27
申请人： Soumitra Roy , James M. Wilson , Luc H. Vandenberghe
发明人： Soumitra Roy , James M. Wilson , Luc H. Vandenberghe
IPC分类号： C12N15/861 , C12N7/01 , C07K14/075 , C12N15/33
CPC分类号： C12N15/86 , A61K39/145 , A61K48/00 , A61K2039/5256 , C07K14/005 , C12N7/00 , C12N2710/10043 , C12N2710/10321 , C12N2710/10322 , C12N2710/10343 , C12N2810/6018
摘要： Novel simian adenovirus 41 and two isolates thereof are described. Various uses of these isolates, including construction of a recombinant vector which comprises simian adenovirus 41 sequences and a heterologous gene under the control of regulatory sequences are provided. A cell line which expresses simian adenovirus 41 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
摘要翻译：描述了新型猿猴腺病毒41及其两个分离物。提供这些分离物的各种用途，包括构建包含猿猴腺病毒41序列和在调控序列控制下的异源基因的重组载体。还公开了表达猿腺病毒41基因的细胞系。提供了使用载体和细胞系的方法。

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