专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

61. 发明申请

US20060211000A1 Methods, compositions, and kits for detection of microRNA 审中-公开
标题翻译：用于检测microRNA的方法，组合物和试剂盒
公开(公告)号：US20060211000A1
公开(公告)日：2006-09-21
申请号：US11084082
申请日：2005-03-21
申请人： Joseph Sorge , Rebecca Mullinax
发明人： Joseph Sorge , Rebecca Mullinax
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6809 , C12Q1/6816 , C12Q2533/107 , C12Q2545/114 , C12Q2525/207
摘要： The present invention provides methods, nucleic acids, compositions, and kits for detecting microRNA (miRNA) in samples. The methods comprise ligating two oligonucleotides together in an miRNA mediated fashion, and detection of the ligation product. The methods can further comprise amplification of the ligation product, such as by PCR. The nucleic acids, compositions, and kits typically comprise some or all of the components necessary to practice the method of the invention.
摘要翻译：本发明提供了用于在样品中检测微小RNA（miRNA）的方法，核酸，组合物和试剂盒。所述方法包括以miRNA介导的方式将两个寡核苷酸结合在一起，并检测连接产物。所述方法可以进一步包括连接产物的扩增，例如通过PCR扩增。核酸，组合物和试剂盒通常包括实施本发明方法所需的一些或全部成分。

62. 发明申请

US20060088822A1 Novel polymerase compositions and uses thereof 失效
标题翻译：新型聚合酶组合物及其用途
公开(公告)号：US20060088822A1
公开(公告)日：2006-04-27
申请号：US10319778
申请日：2002-12-13
申请人： Joseph Sorge , Rebecca Mullinax
发明人： Joseph Sorge , Rebecca Mullinax
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/22
CPC分类号： C12Q1/686 , C12Q2521/319 , C12Q2521/101
摘要： The subject invention provides novel compositions containing a mixture of (a) an enzyme that possesses substantial 3′-5′ exonuclease activity (b) a DNA polymerase with less 3′-5′ exonuclease activity than the enzyme with substantial 3′-5′ exonuclease activity. Preferably, the DNA polymerase for inclusion in the compositions are DNA polymerases that substantially lack 3′-5′ exonuclease activity. A preferred embodiment of the invention is a composition comprising the Taq DNA polymerase (isolated from Thermus aquaticus) and the Pfu DNA polymerase (isolated from Pyrococcus furiosus). Another aspect of the invention is to provide methods for synthesizing polynucleotides, typically DNA, using compositions comprising an enzyme that possesses substantial 3′-5′ exonuclease activity and a DNA polymerase with less 3′-5′ exonuclease activity than the enzymes possessing substantial 3′-5′ exonuclease activity, preferably a DNA polymerase that substantially lacks 3′-5′ exonuclease activity. Another aspect of the invention involves the use the subject method of polynucleotide synthesis to carry out the synthesis step in a polymerase chain reaction experiment. Yet another aspect of the invention is to provide kits for the synthesis of polynucleotides, wherein the kits comprise an enzyme that possesses substantial 3′-5′ exonuclease activity and a DNA polymerase with less 3′-5′ exonuclease activity than the enzyme possessing substantial 3′-5′ exonuclease activity.
摘要翻译：本发明提供了包含（a）具有大量3'-5'核酸外切酶活性的酶的混合物的新型组合物（b）具有比实际3'-5'核酸外切酶活性大的3'-5'核酸外切酶活性的3' 核酸外切酶活性。优选地，包含在组合物中的DNA聚合酶是基本上缺乏3'-5'核酸外切酶活性的DNA聚合酶。本发明的优选实施方案是包含Taq DNA聚合酶（从Thermus aquaticus分离）和Pfu DNA聚合酶（从激烈热球菌分离的）的组合物。本发明的另一方面是提供使用包含具有大量3'-5'核酸外切酶活性的酶的组合物和具有比具有实质3的酶的3'-5'外切核酸酶活性少的DNA聚合酶合成多核苷酸（通常为DNA）的方法 '-5'核酸外切酶活性，优选基本上缺乏3'-5'核酸外切酶活性的DNA聚合酶。本发明的另一方面涉及使用聚合酶链反应实验中的多核苷酸合成的主题方法进行合成步骤。本发明的另一方面是提供用于合成多核苷酸的试剂盒，其中所述试剂盒包含具有显着3'-5'核酸外切酶活性的酶和具有比具有实质性的酶的酶更少3'-5'外切核酸酶活性的DNA聚合酶 3'-5'核酸外切酶活性。

63. 发明申请

US20060035215A9 METHODS FOR DETECTION OF A TARGET NUCLEIC ACID BY CAPTURE 有权
标题翻译：通过捕获来检测目标核酸的方法
公开(公告)号：US20060035215A9
公开(公告)日：2006-02-16
申请号：US09728574
申请日：2000-11-30
申请人： Joseph Sorge , Anne Whalen
发明人： Joseph Sorge , Anne Whalen
IPC分类号： C12Q1/68 , G06F19/00 , G01N33/48 , G01N33/50
CPC分类号： C12Q1/6823 , C12Q1/6816 , C12Q2521/301 , C12Q2531/113 , C12Q2521/307 , C12Q2525/161 , C12Q2525/301 , C12Q2561/109 , C12Q2565/519 , C12Q2561/101 , C12Q2521/107
摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid and further comprising a binding moiety. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample, and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support. The invention also relates to a method of detecting or measuring a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to a target nucleic acid and comprising a binding moiety, and cleaving the cleavage structure with a nuclease to generate a cleaved nucleic acid fragment and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.
摘要翻译：本发明涉及产生指示样品中目标核酸存在的信号的方法，其中该方法包括通过将包含靶核酸的样品与具有二级结构变化的探针一起孵育形成切割结构探针与靶核酸的结合并且还包含结合部分。本发明还包括用核酸酶切割切割结构以释放核酸片段以产生信号的步骤，其中产生信号指示样品中靶核酸的存在，以及检测和/或测量通过结合部分与固体支持物上的捕获元件结合捕获的片段的量。本发明还涉及一种检测或测量样品中的靶核酸的方法，其中该方法包括通过将含有靶核酸的样品与具有在探针结合后变化的二级结构的探针形成切割结构并且包含结合部分，并用核酸酶切割切割结构以产生切割的核酸片段，并通过结合部分与捕获元件的结合捕获的片段的量，并检测和/或测量坚实的支持。

64. 发明申请

US20050118609A1 Compositions and methods for polynucleotide sequence determination 审中-公开
标题翻译：用于多核苷酸序列测定的组合物和方法
公开(公告)号：US20050118609A1
公开(公告)日：2005-06-02
申请号：US10861928
申请日：2004-06-04
申请人： Joseph Sorge , Bahram Arezi , Holly Hogrefe
发明人： Joseph Sorge , Bahram Arezi , Holly Hogrefe
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6818 , C12Q1/6858 , C12Q2535/125 , C12Q2525/161 , C12Q2565/101
摘要： The present invention relates to a method for identifying a nucleotide at a predetermined location on a target polynucleotide. The method involves single nucleotide extension reaction comprising an oligonucleotide primer comprising a first sequence and a second sequence or a tag. The method may further comprises a probe which hybridizes to the second sequence or an anti-tag molecule which interacts with the tag, where the hybridization or interaction causes a detectable signal transfer which is indicative of the identity of the nucleotide base at the predetermined location. The invention further provides compositions and kits for performing the subject method of the invention.
摘要翻译：本发明涉及鉴定靶多核苷酸上预定位置的核苷酸的方法。该方法涉及包含含有第一序列和第二序列或标签的寡核苷酸引物的单核苷酸延伸反应。该方法还可以包括与第二序列杂交的探针或与标签相互作用的抗标签分子，其中杂交或相互作用引起可检测的信号转移，其指示在预定位置处的核苷酸碱基的身份。本发明还提供了用于实施本发明的主题方法的组合物和试剂盒。

65. 发明授权

US5837288A Methods for storage of sequencing gels and stored sequencing gels used by such methods 失效
标题翻译：用于存储测序凝胶和通过这些方法使用的储存的测序凝胶的方法
公开(公告)号：US5837288A
公开(公告)日：1998-11-17
申请号：US587879
申请日：1996-01-11
申请人： Keith Vincent Sylvester , Joseph Sorge
发明人： Keith Vincent Sylvester , Joseph Sorge
IPC分类号： B01D57/02 , G01N27/447 , C25B1/00 , B01D61/42 , C25B7/00
CPC分类号： B01D57/02 , G01N27/44704
摘要： One aspect of the invention is to provide methods for the storage of electrophoresis gels, particularly, electrophoresis gels adapted for polynucleotide sequencing. The invention provides numerous methods of preparing a packaged electrophoresis gel or gel assembly. The subject methods include the steps of inserting an electrophoresis gel or gel assembly into the interior chamber of a storage container, removing air from the interior chamber, and hermetically sealing the storage container so as to provide a sealed package containing an electrophoresis gel in a reduced atmospheric environment. Preferably, the interior chamber is flushed with an inert gas prior to the sealing process. Another aspect of the invention is to provide packaged electrophoresis gels. The packaged electrophoresis gels include a hermetically sealed storage container having an interior chamber. The interior chamber contains a gel or gel assembly suitable for electrophoresis. The interior chamber of the storage container encloses a reduced atmospheric environment. Additionally, the interior chamber preferably contains a buffer solution. The storage container may comprise two sheets of flexible polymer which are fused to one another at or near the perimeter so as to form an interior chamber.
摘要翻译：本发明的一个方面是提供用于存储电泳凝胶的方法，特别是适用于多核苷酸测序的电泳凝胶。本发明提供了制备包装的电泳凝胶或凝胶组件的许多方法。本发明的方法包括以下步骤：将电泳凝胶或凝胶组合物插入储存容器的内部腔室中，从内部室中除去空气，并密封储存容器，从而提供包含电泳凝胶的密封包装，大气环境。优选地，在密封过程之前，用惰性气体冲洗内部室。本发明的另一方面是提供包装的电泳凝胶。包装的电泳凝胶包括具有内部室的密封储存容器。内室含有适合电泳的凝胶或凝胶组合物。储存容器的内部空间包围了一个减少的大气环境。此外，内部室优选含有缓冲溶液。存储容器可以包括两片柔性聚合物，它们在周边或附近彼此熔合以形成内部室。

66. 发明授权

US5328581A Electrophoresis temperature feedback controller 失效
标题翻译：电泳温度反馈控制器
公开(公告)号：US5328581A
公开(公告)日：1994-07-12
申请号：US643398
申请日：1991-01-18
申请人： Joseph Sorge
发明人： Joseph Sorge
IPC分类号： G01N27/447 , G05D23/20 , G01N27/26 , B01D57/02
CPC分类号： G01N27/44704 , G05D23/1906 , G05D23/20
摘要： A method of (and apparatus for) electrophoresis in which the gel temperature is controlled automatically. A sensor may measure a gel temperature and a processor may control a power supply for the electrophoresis process in response to that measurement. Measurements may be made at predetermined times (e.g., periodically) and the processor may determine control settings for the power supply based on a preferred temperature for the electrophoresis process. The preferred temperature for the electrophoresis process may be a predetermined temperature which is chosen so that the electrophoresis process operates at or near the highest speed which is unlikely to break the glass plates.
摘要翻译：凝胶温度自动控制的（及其电泳装置）方法。传感器可以测量凝胶温度，并且处理器可以响应于该测量来控制电泳过程的电源。可以在预定时间（例如，周期性地）进行测量，并且处理器可以基于电泳过程的优选温度确定供电的控制设置。电泳过程的优选温度可以是预定的温度，其被选择为使得电泳过程以或不接近玻璃板的最高速度运行。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式