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    • 66. 发明公开
    • 블록 공중합체의 나노패턴을 이용한 나노-바이오칩의제조방법
    • 使用嵌段共聚物纳米生物制备纳米生物技术的方法
    • KR1020050033312A
    • 2005-04-12
    • KR1020030069302
    • 2003-10-06
    • 한국과학기술원한국생명공학연구원
    • 정희태정봉현권기영정진미
    • G01N33/544G01N33/554G01N33/553
    • A method for fabricating a nano-biochip by using nano-pattern of block copolymers is provided, thereby immobilizing real-life samples on the surface of the nanometer-sized biochip with high density without loss of tertiary structure of a protein in the real-life samples. The method for fabricating a nano-biochip by using nano-pattern of block copolymers comprises the steps of: (a) forming a metal thin layer with affinity to a bio-receptor on a substrate; (b) coating the metal thin layer with the block copolymers; (c) heating the block copolymers to induce self-assembly of the copolymers and form a regular structure; (d) etching the copolymers to form a porous nano-pattern; and (e) attaching the bio-receptor to the metal exposed by the porous nano-pattern, wherein the bio-receptor is an enzyme substrate, a ligand, an amino acid, a peptide, a protein, a nucleic acid, a lipid, a cofactor or a carbohydrate; the metal with affinity to the bio-receptor is Au, Ag, Pt, Nb, Ta, Zr or alloy of Co and Cr; and the block copolymer is polystyrene-polymethylmethacrylate(PS-PMMA), polystyrene-polybutadiene(PS-PB) or polystyrene-polyisoprene(PS-PI).
    • 提供了一种通过使用嵌段共聚物纳米图案制造纳米生物芯片的方法,从而将现实生活中的样品以高密度固定在纳米尺寸的生物芯片的表面上,而不会损失现实生活中蛋白质的三级结构 样本。 通过使用嵌段共聚物的纳米图案制造纳米生物芯片的方法包括以下步骤:(a)形成与基底上的生物受体具有亲和力的金属薄层; (b)用嵌段共聚物涂覆金属薄层; (c)加热嵌段共聚物以引起共聚物的自组装并形成规则结构; (d)蚀刻共聚物以形成多孔纳米图案; 和(e)将生物受体附着到由多孔纳米图案暴露的金属上,其中生物受体是酶底物,配体,氨基酸,肽,蛋白质,核酸,脂质, 辅助因子或碳水化合物; 对生物受体具有亲和力的金属是Au,Ag,Pt,Nb,Ta,Zr或Co和Cr的合金; 嵌段共聚物是聚苯乙烯 - 聚甲基丙烯酸甲酯(PS-PMMA),聚苯乙烯 - 聚丁二烯(PS-PB)或聚苯乙烯 - 聚异戊二烯(PS-PI)。
    • 67. 发明公开
    • 전도성 탄소나노튜브를 이용한 바이오센서 및 그 제조방법
    • 导电碳纳米管(CNT) - 使用导电碳纳米管的碳纳米管(CNT) - 传感器,其中金属或导电碳纳米管与金属和其制备方法相结合
    • KR1020040107225A
    • 2004-12-20
    • KR1020030038232
    • 2003-06-13
    • 한국과학기술원
    • 이상엽정희태김도현이석재김병훈정대환고영관이재신
    • C12Q1/00B82Y5/00B82Y15/00
    • B82Y30/00B82Y40/00C01B32/168C01B32/174G01N33/551
    • PURPOSE: A conductive carbon nanotube(CNT)-biosensor by using a conductive CNT dotted with metals or a conductive CNT dotted with metals where bioreceptor is combined and a preparation method thereof are provided. The biosensor has large surface area, improved conductivity to increase the amount of immobilized biological molecules, and improved accuracy with a small amount of a sample. CONSTITUTION: The conductive-carbon nanotube(CNT) biosensor has a bioreceptor which binds to or reacts with a target bio-material in metals scattering on the conductive CNT or a pattern of the conductive CNT, wherein the bioreceptor is enzyme substrate, ligand, amino acid, peptide, protein, nucleic acid, lipid, cofactor or carbohydrate; and the conductive CNT dotted with metals has a form of CNT-(CONH-R1-S-M)r in which M is metal, r is an integer of 1 or over, and R1 is C1-20 carbohydrate, unsaturated carbohydrate or aromatic organic group. The method for preparing the conductive CNT-biosensor comprises binding a conductive CNT-M-nucleic acid complex to a substrate with amine/lysine group on its surface through UV radiation.
    • 目的:提供一种导电性碳纳米管(CNT) - 双极性传感器,其通过使用点状金属的导电性CNT或带有生物受体结合的金属的导电性CNT及其制备方法。 生物传感器具有较大的表面积,改善的电导率以增加固定的生物分子的数量,并且用少量样品提高了精度。 构成:导电碳纳米管(CNT)生物传感器具有生物受体,其在导电CNT上散射的金属或导电CNT的图案上与靶生物材料结合或与靶生物材料反应,其中生物受体是酶底物,配体,氨基 酸,肽,蛋白质,核酸,脂质,辅因子或碳水化合物; 并且金属点缀的导电CNT具有CNT-(CONH-R1-SM)r的形式,其中M是金属,r是1或更大的整数,R1是C1-20碳水化合物,不饱和碳水化合物或芳族有机基团 。 制备导电CNT-生物传感器的方法包括通过UV辐射将导电CNT-M-核酸复合物与其表面上的胺/赖氨酸基团结合到底物上。
    • 68. 发明公开
    • 10나노 이하 사이즈의 바이오 나노어레이 제조방법
    • 通过在纳米尺寸图上固定生物体或生物受体,生产小于10纳米的生物纳米粒子的制造方法
    • KR1020040105320A
    • 2004-12-16
    • KR1020030036531
    • 2003-06-05
    • 한국과학기술원
    • 정희태권기영이수림
    • B82B3/00B82Y15/00B82Y5/00B82Y40/00
    • B82B3/0095B82Y15/00B82Y40/00
    • PURPOSE: Provided is a method for manufacturing bio nanoarrays by fixing biomaterial or bio-receptor on a nano-sized pattern based on self-assembly of polymeric supramolecules and stanning of metal. CONSTITUTION: The bio nanoarrays are manufactured by the following steps of: (i) forming a thin film of organic molecules inducing self-assembly on a substrate formed by spin-coating, rubbing or solution spreading; (ii) annealing the thin film to form ordered structure by self-assembling organic molecules, wherein the organic molecules are annealed by heating to 240deg.C, higher than phase transition temperature(230deg.C) of the liquid crystal and cooling; (iii) adsorbing metals selectively on the ordered structure formed in self-assembling by using RuO4; (iv) etching the thin film through reactive ion etching and/or ion milling to remove the parts on which the metal is not adsorbed, resulting in formation of a nano-sized pattern of organic molecules; (v) printing the nano-sized pattern having a uneven hole or columnar shape on the substrate; (vi) fixing biomaterial or bio-receptor bonding to biomaterials on the nano-sized pattern due to the amine-aldehyde reaction between amine group on the ends of biomaterial or bio-receptor and aldehyde on the surface of substrate, wherein the biomaterials are selected from the group consisting of protein, peptide, amino acid, ligand, carbohydrate, DNA, oligonucleotide and RNA.
    • 目的:提供一种通过基于聚合物超分子的自组装和金属鞣制将生物材料或生物受体固定在纳米尺寸图案上来制造生物纳米阵列的方法。 构成:通过以下步骤制造生物纳米阵列:(i)在通过旋涂,摩擦或溶液扩散形成的基底上形成诱导自组装的有机分子的薄膜; (ii)通过自组装有机分子对薄膜进行退火以形成有序结构,其中有机分子通过加热退火至240℃,高于液晶的相变温度(230℃)并冷却; (iii)通过使用RuO4在自组装形成的有序结构上选择性地吸附金属; (iv)通过反应离子蚀刻和/或离子研磨来蚀刻薄膜以去除不吸附金属的部分,形成纳米尺度的有机分子图案; (v)在基板上印刷具有不均匀孔或柱状的纳米尺寸图案; (vi)由于生物材料或生物受体的端部上的胺基与底物表面上的醛之间的胺 - 醛反应,将生物材料或生物受体结合固定在纳米尺寸图案上的生物材料上,其中选择生物材料 来自由蛋白质,肽,氨基酸,配体,碳水化合物,DNA,寡核苷酸和RNA组成的组。
    • 69. 发明公开
    • 탄소나노튜브 어레이의 제작방법
    • 碳纳米管在纳米尺寸图上的对比制备碳纳米管阵列
    • KR1020040105319A
    • 2004-12-16
    • KR1020030036530
    • 2003-06-05
    • 한국과학기술원
    • 정희태정대환권기영이수림최도환
    • B82B3/00B82Y15/00B82Y5/00B82Y40/00
    • B82Y30/00B82Y40/00C01B32/162C01B2202/08
    • PURPOSE: Provided is a simple method for manufacturing carbon nanotube(CNT) arrays by aligning carbon nanotubes on a nano-sized pattern based on self-assembly of polymeric supramolecules and stanning of metal. CONSTITUTION: The carbon nanotube arrays are manufactured by the following steps of: (i) forming a thin film of polymeric supramolecules inducing self- assembly on a substrate formed by spin-coating, rubbing or solution spreading, wherein the polymeric supramolecules are disk-shaped dendrimer, fan or corn-shaped supramolecules; (ii) annealing the thin film to form ordered structure by self-assembling polymeric supramolecules, wherein the supramolecules are annealed by heating to 240deg.C, higher than phase transition temperature(230deg.C) of the liquid crystal and cooling; (iii) adsorbing metals selectively on the ordered structure formed in self-assembling by using RuO4; (iv) reactive ion etching the thin film to remove the parts on which the metal is not adsorbed, resulting in formation of a nano-sized pattern of supramolecules; (v) printing the nano-sized pattern on the substrate; (vi) arraying carbon nanotubes on the pattern after depositing metal catalyst(Fe, Co or Ni) on the patterned substrate. Also, bio-nanoarrays are manufactured by attaching biomaterials or bio-receptor, bonding to biomaterials selected from the group consisting of protein, peptide, amino acid, DNA, ligand, carbo hydrate, RNA, etc. to CNT arrays.
    • 目的:提供一种简单的制造碳纳米管(CNT)阵列的方法,其通过基于聚合超分子的自组装和金属扫描将纳米尺寸图案上的碳纳米管取向。 构成:通过以下步骤制造碳纳米管阵列:(i)在通过旋涂,摩擦或溶液扩散形成的基底上形成诱导自组装的聚合物超分子的薄膜,其中聚合物超分子是盘形的 树枝状大分子,扇形或玉米状超分子; (ii)通过自组装聚合物超分子对薄膜进行退火以形成有序结构,其中超分子通过加热退火至240℃,高于液晶的相变温度(230℃)并冷却; (iii)通过使用RuO4在自组装形成的有序结构上选择性地吸附金属; (iv)反应离子蚀刻薄膜以去除不吸附金属的部分,形成超分子的纳米尺寸图案; (v)在衬底上印刷纳米尺寸图案; (vi)在图案化衬底上沉积金属催化剂(Fe,Co或Ni)之后,在图案上排列碳纳米管。 另外,生物纳米阵列是通过将生物材料或生物受体连接到碳纳米管阵列上,与选自蛋白质,肽,氨基酸,DNA,配体,碳水化合物,RNA等的生物材料结合而制造的。